FastQCFastQC Report
Thu 26 May 2016
SRR936777_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936777_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461813
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135000.9235107363253713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89780.6141688437577173No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64890.4439008272603951No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.138731835056878No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18920.12942831949093353No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18310.12525541912679666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.1240924796810536No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15800.10808496025141383No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA110650.035.2260971
GGTATCA67750.035.0100981
TATCAAC133050.029.0464212
TCAACGC132700.028.9797444
ATCAACG133650.028.8448893
CAACGCA134450.028.708615
AACGCAG137200.028.3035096
ACGCAGA149950.025.7384227
CGCAGAG151150.025.2816498
GTACATG78800.025.154281
GAGTACT78350.024.6528712-13
GCAGAGT156900.023.8066129
TACATGG83400.023.3687112
GTATAGG5300.023.3184431
ACATGGG82500.023.1050763
AGTACTT82500.023.0959812-13
GTACTTT85150.022.59692814-15
CATGGGG33000.021.8950844
CATATAG4300.021.0040043
ACTTTTT97950.019.98214316-17
Produced by FastQC (version 0.11.5)