FastQCFastQC Report
Thu 26 May 2016
SRR936777_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936777_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1461813
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117880.8063958933187761No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88890.6080805137182389No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65410.4474580538003151No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27990.19147455933146032No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24630.1684894032273622No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20010.13688481358422724No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19120.13079648354474888No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16480.11273671803438608No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA112900.033.748911
GGTATCA72200.031.9799941
TCAACGC140150.026.9404454
ATCAACG140550.026.8975663
TATCAAC141950.026.7019392
CAACGCA142850.026.3665545
AACGCAG146700.025.7393446
ACGCAGA160700.023.2014057
CGCAGAG162500.022.8567168
GAGTACT80700.022.71666312-13
GTACATG86100.022.4027961
GCAGAGT165950.021.8663629
AGTACTT85350.021.7850712-13
TACTCCC5200.021.008015
TACATGG91050.020.9711112
CCCTATA4100.020.852782
GTACTTT87700.020.8500314-15
ACATGGG88250.020.7731973
AGAGTAC150850.019.42675810-11
CATGGGG33950.019.0252294
Produced by FastQC (version 0.11.5)