FastQCFastQC Report
Thu 26 May 2016
SRR936776_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936776_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477925
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137410.929749479845053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93070.6297342557978246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64970.4396028215234197No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20130.13620447586988513No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19560.13234771723869615No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18950.12822030887900265No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.12679939780435406No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA15510.10494443222761642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65750.036.6450961
GTATCAA107350.035.592431
ATCAACG130700.029.0548733
TCAACGC130750.029.0437624
TATCAAC131050.028.9410082
CAACGCA132050.028.6839055
AACGCAG133350.028.3676726
ACGCAGA146500.025.5259517
CGCAGAG146800.025.2795758
GAGTACT76900.025.24208512-13
GTACATG81150.024.8302421
GCAGAGT151900.023.7097429
ACATGGG84600.023.0335713
TACATGG87350.022.8525222
GTACTTT85650.022.74619114-15
AGTACTT81900.022.51165212-13
CATGGGG33500.021.5648564
CATGGGA50000.021.1023464
ACTTTTT95600.020.62625116-17
CTTACTA6600.020.1571679
Produced by FastQC (version 0.11.5)