##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936776_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1477925 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90290779302062 33.0 31.0 34.0 30.0 34.0 2 31.996424040462134 33.0 31.0 34.0 30.0 34.0 3 32.00758157551973 33.0 31.0 34.0 30.0 34.0 4 35.62308303871983 37.0 35.0 37.0 33.0 37.0 5 35.63221814368117 37.0 35.0 37.0 33.0 37.0 6 35.70455469661857 37.0 35.0 37.0 33.0 37.0 7 35.655322834379284 37.0 35.0 37.0 33.0 37.0 8 35.62558113571392 37.0 35.0 37.0 33.0 37.0 9 37.2627575824213 39.0 37.0 39.0 34.0 39.0 10-11 37.15196677774583 39.0 37.0 39.0 33.5 39.0 12-13 37.07140314968622 39.0 37.0 39.0 33.0 39.0 14-15 38.20909992049664 40.0 38.0 41.0 33.0 41.0 16-17 38.15454099497606 40.0 38.0 41.0 33.0 41.0 18-19 38.15393067983829 40.0 38.0 41.0 33.5 41.0 20-21 38.11640847810274 40.0 38.0 41.0 33.5 41.0 22-23 38.01037231253277 40.0 38.0 41.0 33.0 41.0 24-25 37.9667405314884 40.0 38.0 41.0 33.0 41.0 26-27 37.966909349256554 40.0 38.0 41.0 33.0 41.0 28-29 37.881717610839516 40.0 38.0 41.0 33.0 41.0 30-31 37.69079723260653 40.0 37.0 41.0 33.0 41.0 32-33 37.46423431500245 40.0 36.5 41.0 33.0 41.0 34-35 37.28854813336265 40.0 36.0 41.0 32.0 41.0 36-37 37.09848165502309 39.0 36.0 41.0 31.0 41.0 38-39 36.933727354229745 39.0 36.0 41.0 31.0 41.0 40-41 36.73659522641542 39.0 35.0 41.0 31.0 41.0 42-43 36.498161611719134 39.0 35.0 41.0 30.0 41.0 44-45 36.194312972579795 39.0 35.0 41.0 30.0 41.0 46-47 35.788881370840876 39.0 35.0 40.0 28.5 41.0 48-49 35.49245733037874 39.0 35.0 40.0 27.0 41.0 50-51 34.772970888238575 38.0 34.0 40.0 26.0 40.5 52-53 34.94476614171896 38.5 34.5 40.0 27.0 40.5 54-55 35.32660283843903 39.0 35.0 40.5 27.0 41.0 56-57 35.21968333981765 39.0 35.0 41.0 27.0 41.0 58-59 35.00961855303889 38.5 35.0 40.0 26.0 41.0 60-61 34.73675051169714 38.0 34.5 40.0 26.0 41.0 62-63 34.43040208400291 38.0 34.0 40.0 26.0 41.0 64-65 34.09876380736506 37.0 34.0 40.0 25.0 41.0 66-67 33.73971717103371 36.5 34.0 39.5 23.5 41.0 68-69 33.353746638022905 36.0 33.0 39.0 23.0 41.0 70-71 32.952335199688754 35.5 33.0 39.0 22.0 40.5 72-73 32.57758884923119 35.0 33.0 38.0 21.5 40.0 74-75 32.193251010707584 35.0 32.5 37.0 20.5 39.5 76-77 31.748280866755756 35.0 32.0 37.0 19.0 39.0 78-79 31.307304836172335 35.0 31.5 36.0 17.5 39.0 80-81 30.889986974981817 35.0 31.0 36.0 13.5 37.5 82-83 30.509291066867398 35.0 31.0 35.5 8.5 37.0 84-85 30.153908689547848 34.0 31.0 35.0 7.0 37.0 86-87 29.817240387705738 34.0 30.0 35.0 2.0 36.0 88-89 29.464265778033393 34.0 30.0 35.0 2.0 36.0 90-91 29.11647478728623 34.0 29.5 35.0 2.0 35.5 92-93 28.757346956036336 34.0 29.0 35.0 2.0 35.0 94-95 28.40224774599523 34.0 29.0 35.0 2.0 35.0 96-97 27.936065091259707 34.0 29.0 35.0 2.0 35.0 98-99 27.40751695789705 34.0 27.5 35.0 2.0 35.0 100-101 25.30352588933809 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29473992917411707 1101 2 0.3005000976396168 1101 3 0.20779530069538765 1101 4 0.26179716792611885 1101 5 0.27202034836847133 1101 6 0.08955112924743958 1101 7 0.17163278432959572 1101 8 0.11029688616146416 1101 9 0.20449857449941788 1101 10-11 0.3320656108181055 1101 12-13 0.40329056962182364 1101 14-15 0.42378959421672846 1101 16-17 0.47651395568215804 1101 18-19 0.420428328335511 1101 20-21 0.42984734436947036 1101 22-23 0.4280653348484762 1101 24-25 0.40482872928416214 1101 26-27 0.3311312787506395 1101 28-29 0.3420111448398373 1101 30-31 0.47075003291164563 1101 32-33 0.337809047228383 1101 34-35 0.4078645130438474 1101 36-37 0.39174282353864953 1101 38-39 0.3763745688472824 1101 40-41 0.4117690148194768 1101 42-43 0.3213873838800154 1101 44-45 0.44965795916420603 1101 46-47 0.38963859292727676 1101 48-49 0.17284175564640236 1101 50-51 0.33318061327378956 1101 52-53 0.3320344644599018 1101 54-55 0.33327852936658076 1101 56-57 0.32608913385178084 1101 58-59 0.3052575610600954 1101 60-61 0.3874117913022488 1101 62-63 0.44005442699396724 1101 64-65 0.35826267799055955 1101 66-67 0.383940428387767 1101 68-69 0.3483604870792405 1101 70-71 0.34074154004402857 1101 72-73 0.28223248506730414 1101 74-75 0.40767654700852773 1101 76-77 0.550783141566626 1101 78-79 0.41458976509852263 1101 80-81 0.4641332471993742 1101 82-83 0.41057254339788685 1101 84-85 0.3591744992230801 1101 86-87 0.5592770331931582 1101 88-89 0.29754699196615064 1101 90-91 0.4073522073775315 1101 92-93 0.3483597603002444 1101 94-95 0.5330385330599761 1101 96-97 0.37996321774783226 1101 98-99 0.40751790687369294 1101 100-101 0.7131617721829393 1102 1 0.14096347849720203 1102 2 0.08543963603181837 1102 3 0.17715193363535775 1102 4 0.12723417851795915 1102 5 0.14013425392554524 1102 6 0.05040596556334975 1102 7 0.07882717782771209 1102 8 0.007876379457648852 1102 9 0.21385341013452575 1102 10-11 0.06995456733286431 1102 12-13 0.0616886127380738 1102 14-15 0.15596257663990798 1102 16-17 0.20160664623391966 1102 18-19 -0.13988146166575177 1102 20-21 0.04449190690954907 1102 22-23 0.08852210898580637 1102 24-25 -0.06504156521513238 1102 26-27 0.10449624192632001 1102 28-29 -0.004809123312597308 1102 30-31 0.12211748235903741 1102 32-33 -0.011719432564873955 1102 34-35 -0.07546820757191597 1102 36-37 -0.0209721552272768 1102 38-39 0.0781547176151065 1102 40-41 -0.1707031099352676 1102 42-43 -0.026235544283615297 1102 44-45 0.0600754512975854 1102 46-47 0.30053277932685063 1102 48-49 0.2792922419399133 1102 50-51 0.1676832159618442 1102 52-53 0.25596625946366913 1102 54-55 0.006080200860097307 1102 56-57 0.12938500971792166 1102 58-59 -0.1784992962706582 1102 60-61 -0.0039057284715013907 1102 62-63 -0.13765586107859917 1102 64-65 0.021219339796331838 1102 66-67 -0.23408293112903777 1102 68-69 -0.2507782362624411 1102 70-71 -0.1822813224907378 1102 72-73 -0.3257116704921543 1102 74-75 -0.11422912716461298 1102 76-77 -0.23070917972068372 1102 78-79 -0.23188309219411352 1102 80-81 0.010646680010811593 1102 82-83 0.22402693610627722 1102 84-85 0.07150190008877999 1102 86-87 0.02154758748470087 1102 88-89 0.155446895701413 1102 90-91 0.09933261947774952 1102 92-93 0.11401775769415323 1102 94-95 0.1900300489640827 1102 96-97 -0.05430171860442812 1102 98-99 0.09845397592287952 1102 100-101 0.35306391437970674 1103 1 0.19754032014342116 1103 2 0.26827646639887703 1103 3 0.1920645201302449 1103 4 0.1040506552469509 1103 5 -0.14617879261916755 1103 6 0.09398687066898503 1103 7 0.13670623775397672 1103 8 0.22448272459192964 1103 9 0.17080214109420666 1103 10-11 0.19598742495833932 1103 12-13 0.10827976196085132 1103 14-15 0.29556979799548344 1103 16-17 0.3216573755434524 1103 18-19 0.29635291339733527 1103 20-21 0.23288722403840012 1103 22-23 0.2901209773009228 1103 24-25 0.17169061391088292 1103 26-27 0.179107292543911 1103 28-29 0.21225754084160542 1103 30-31 0.2200806379939806 1103 32-33 0.1984545599488854 1103 34-35 0.2353369738435731 1103 36-37 0.13965596488098697 1103 38-39 0.22723784826898452 1103 40-41 0.21673273814868566 1103 42-43 0.30182991792647584 1103 44-45 0.3194396457460371 1103 46-47 0.32614441812985717 1103 48-49 0.33350153634623325 1103 50-51 0.08435638811174329 1103 52-53 0.23198592666108908 1103 54-55 0.42921735573860786 1103 56-57 0.29335178868268486 1103 58-59 0.2866648505307339 1103 60-61 0.19724796289670365 1103 62-63 0.43616073544101397 1103 64-65 0.37196527196253726 1103 66-67 0.26801609668257953 1103 68-69 0.3033193432940138 1103 70-71 0.015954659651036707 1103 72-73 -0.0901464404605079 1103 74-75 -0.07792112797757511 1103 76-77 0.15391596311953748 1103 78-79 0.08916115900009558 1103 80-81 0.08888072396723956 1103 82-83 0.14833575094631613 1103 84-85 0.05885120281314471 1103 86-87 0.2232167823167437 1103 88-89 0.09807997433386362 1103 90-91 0.16824587626173582 1103 92-93 0.33227728071212326 1103 94-95 0.3588655091417756 1103 96-97 0.5121790482709088 1103 98-99 0.13208956746646905 1103 100-101 0.2002649190057788 1104 1 0.14953565313400574 1104 2 0.07050395114185548 1104 3 0.038804406278657666 1104 4 0.09841002029925505 1104 5 -0.05962135137633595 1104 6 -0.05460623377133089 1104 7 -0.03926292588703717 1104 8 0.046254643153680775 1104 9 0.0914649789662434 1104 10-11 0.2199108428501475 1104 12-13 0.07275641492661578 1104 14-15 0.04088014612987223 1104 16-17 0.2363016492667498 1104 18-19 0.03729959441310626 1104 20-21 0.020908566549472596 1104 22-23 -0.05556570174362463 1104 24-25 0.0033712700625656566 1104 26-27 0.013275954854350402 1104 28-29 0.19563464958665122 1104 30-31 0.27920907665023975 1104 32-33 0.15159933047426932 1104 34-35 0.17827965380446642 1104 36-37 0.10991942981581815 1104 38-39 0.044570846849175894 1104 40-41 0.005605836515506724 1104 42-43 0.1406699183526925 1104 44-45 0.037979287355945246 1104 46-47 0.12176696575226487 1104 48-49 0.1457170072693259 1104 50-51 0.03466252741344533 1104 52-53 0.09013860872639867 1104 54-55 0.31048998154408025 1104 56-57 0.33243899143387523 1104 58-59 0.3162269055066105 1104 60-61 0.26114929773599016 1104 62-63 0.18154034284749798 1104 64-65 0.18493726724225468 1104 66-67 0.13269679811918422 1104 68-69 0.033223499116715516 1104 70-71 0.10377641558668671 1104 72-73 0.05881693470364979 1104 74-75 0.06449724597455031 1104 76-77 0.040612455670171244 1104 78-79 0.23773961244054576 1104 80-81 0.0475619075549254 1104 82-83 -0.015054863356787962 1104 84-85 0.05931004549070806 1104 86-87 0.2694616644561094 1104 88-89 0.04438738960446642 1104 90-91 -0.026523849977671432 1104 92-93 -0.12359590556069122 1104 94-95 0.10189529595640678 1104 96-97 0.029604747722959956 1104 98-99 0.14705989253219798 1104 100-101 0.24628437553360172 1105 1 0.09048604366906687 1105 2 0.16478482303028485 1105 3 0.1404041490554988 1105 4 0.0944188378220332 1105 5 0.07039249071752351 1105 6 0.047571852395705605 1105 7 0.051873501844561076 1105 8 0.16219580335548045 1105 9 0.19114815033164234 1105 10-11 0.1307272607178831 1105 12-13 0.06035949833476195 1105 14-15 0.165299248219398 1105 16-17 0.3838522730465428 1105 18-19 0.15802809591107803 1105 20-21 0.14922450416739963 1105 22-23 0.2183819040237367 1105 24-25 0.15222759430544386 1105 26-27 0.2231362668736736 1105 28-29 0.1951257891479372 1105 30-31 0.33132418537744 1105 32-33 0.2611753073568863 1105 34-35 0.18583916050276628 1105 36-37 0.026037328760359912 1105 38-39 0.3344082499306751 1105 40-41 0.29558612243103966 1105 42-43 0.5511528861739095 1105 44-45 0.5660544869831838 1105 46-47 0.6048185752362869 1105 48-49 0.4702575682828467 1105 50-51 0.43029113013854214 1105 52-53 0.6030387658123644 1105 54-55 0.3965379751898013 1105 56-57 0.4931484600527938 1105 58-59 0.6350250752439237 1105 60-61 0.6285610101092658 1105 62-63 0.6167682673644777 1105 64-65 0.6643246728442094 1105 66-67 0.6754073553043298 1105 68-69 0.6207113906906159 1105 70-71 0.41356291900021347 1105 72-73 0.49647199408432385 1105 74-75 0.5951930428009433 1105 76-77 0.4104868958896333 1105 78-79 0.4863465473840698 1105 80-81 0.2878886204799791 1105 82-83 0.33008262433042646 1105 84-85 0.4226977072259004 1105 86-87 0.4938607731669542 1105 88-89 0.42863798872462056 1105 90-91 0.552403333251597 1105 92-93 0.48631624619007496 1105 94-95 0.485189993589767 1105 96-97 0.4703454972941401 1105 98-99 0.5282057277104641 1105 100-101 0.6432956549327216 1106 1 0.286084713078953 1106 2 0.2554087183343192 1106 3 0.24041509432765906 1106 4 0.19159732415005948 1106 5 0.17017491645606952 1106 6 0.17854904333597688 1106 7 0.31844745416755416 1106 8 0.27112270816563466 1106 9 0.3917054717573052 1106 10-11 0.4191392496365367 1106 12-13 0.38681647675926456 1106 14-15 0.2236035297436345 1106 16-17 0.34297685834645364 1106 18-19 0.23745213054547065 1106 20-21 0.13589290383549724 1106 22-23 0.06676418540302365 1106 24-25 0.03332255785324634 1106 26-27 0.03726021616451902 1106 28-29 -0.011165043003359187 1106 30-31 -0.022944692628627195 1106 32-33 0.004189376468879402 1106 34-35 -0.03206752661479584 1106 36-37 5.945629196730806E-4 1106 38-39 -0.14599046858502618 1106 40-41 -0.09005944894914819 1106 42-43 -0.09340291326729044 1106 44-45 0.008650417116847109 1106 46-47 0.17793576656111298 1106 48-49 0.016434599185494392 1106 50-51 0.0883613825158136 1106 52-53 0.21547684737239337 1106 54-55 0.28967625095445015 1106 56-57 0.21119029909516485 1106 58-59 0.2586284437253852 1106 60-61 0.269230498388211 1106 62-63 0.1579038171647369 1106 64-65 0.06384929305066578 1106 66-67 0.08082282452370748 1106 68-69 0.2654531858039988 1106 70-71 0.2805196230533582 1106 72-73 0.1419827315442248 1106 74-75 0.41740872826341047 1106 76-77 0.40959648320849595 1106 78-79 0.4792040177541814 1106 80-81 0.46244703791195363 1106 82-83 0.3412214725032321 1106 84-85 0.46701034413424836 1106 86-87 0.5366029853839507 1106 88-89 0.5220176151358622 1106 90-91 0.5925887520783526 1106 92-93 0.719364201273045 1106 94-95 0.8419132379459242 1106 96-97 0.8105086558038685 1106 98-99 0.927344305209644 1106 100-101 1.038145608563223 1107 1 0.23542481593370113 1107 2 0.32572285823775715 1107 3 0.1997536884712332 1107 4 0.2417373719040512 1107 5 0.2842449600119039 1107 6 0.307675040397271 1107 7 0.2929420359265791 1107 8 0.28023237178864235 1107 9 0.35612776657258394 1107 10-11 0.3115566640991787 1107 12-13 0.39691172235120575 1107 14-15 0.3177594058456492 1107 16-17 0.4372688464341863 1107 18-19 0.5677565622205307 1107 20-21 0.561339613533228 1107 22-23 0.5638279382892506 1107 24-25 0.5555301302713858 1107 26-27 0.623045205144372 1107 28-29 0.4717873407602866 1107 30-31 0.5685004230829591 1107 32-33 0.5623378926842477 1107 34-35 0.6821481403667349 1107 36-37 0.6552899344561922 1107 38-39 0.6857849564779457 1107 40-41 0.871374941793924 1107 42-43 0.9931274118271674 1107 44-45 0.9374605687751156 1107 46-47 0.9592308639057876 1107 48-49 0.8993815712677815 1107 50-51 0.8110463308260591 1107 52-53 0.9314135930168064 1107 54-55 0.8122716605367373 1107 56-57 0.8423095987907985 1107 58-59 0.813892369785421 1107 60-61 0.8043506705124699 1107 62-63 0.8707706635648194 1107 64-65 0.7795580921165737 1107 66-67 0.8542488451996135 1107 68-69 0.7976075925525308 1107 70-71 0.7304500911474037 1107 72-73 0.6501765090945995 1107 74-75 0.8628352015526559 1107 76-77 0.6612560654922888 1107 78-79 0.6676748405921309 1107 80-81 0.6283363903468775 1107 82-83 0.8400604208659495 1107 84-85 0.7510153819176217 1107 86-87 1.0265151193575015 1107 88-89 0.8268994436937938 1107 90-91 0.788677746358367 1107 92-93 0.9493336006748088 1107 94-95 1.2550811706180482 1107 96-97 1.1359360146409792 1107 98-99 1.190886627837692 1107 100-101 1.2925151312857288 1108 1 -0.012148893373272074 1108 2 0.11092786809895117 1108 3 0.1826413176653645 1108 4 0.18420892735142047 1108 5 0.0811172294596787 1108 6 0.17311810760318025 1108 7 0.10151979269210187 1108 8 0.0769439287339182 1108 9 -0.053708228659090196 1108 10-11 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2611.0 3 879.0 4 873.0 5 956.0 6 1093.0 7 1213.0 8 1537.0 9 1923.0 10 2417.0 11 3199.0 12 4627.0 13 5763.0 14 6585.0 15 8655.0 16 11933.0 17 16848.0 18 14399.0 19 7708.0 20 7240.0 21 7609.0 22 7999.0 23 8721.0 24 9993.0 25 11653.0 26 14022.0 27 16454.0 28 19980.0 29 24544.0 30 30512.0 31 38828.0 32 49812.0 33 67168.0 34 93986.0 35 141243.0 36 208610.0 37 300942.0 38 276218.0 39 49164.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.51863132900682 19.564790673370318 15.137633308154033 21.778944689468823 2 14.990880018950609 20.964518366863505 40.63511615708702 23.409485457098864 3 18.093750465287208 26.834720509589317 30.857982839531072 24.213546185592406 4 12.33269520810374 17.590729764344957 38.50034252973781 31.576232497813493 5 12.972302341220473 37.95058047991938 34.20947662334936 14.867640555510784 6 29.32542738646547 37.70321135064216 18.19580223482532 14.77555902806706 7 26.842872394368296 34.34197369641407 21.755598837480882 17.059555071736746 8 26.16639782871027 33.8441254504898 20.471976521033692 19.51750019976624 9 27.017558826775435 16.54922639734962 21.18068603533974 35.25252874053521 10-11 23.948954667560418 27.234773659416796 27.805792111637544 21.010479561385246 12-13 25.687188228383977 25.16516384354841 28.27601616176662 20.87163176630099 14-15 22.915798506793227 25.700725615215745 27.337870614699504 24.04560526329152 16-17 20.91272860693958 29.271774861766254 27.746194171707707 22.069302359586462 18-19 20.952123687526107 27.201527070017338 31.215501967737637 20.63084727471892 20-21 21.811941845473008 26.343150432916186 31.190241098722627 20.654666622888183 22-23 21.692259897636102 26.239581880967112 31.07476002961812 20.99339819177867 24-25 21.297007127851675 26.68026576090248 31.166917998409115 20.85580911283673 26-27 21.194358275680425 26.667530515809286 31.206155567953182 20.93195564055711 28-29 20.874971241423175 26.45609749495879 31.614032832144645 21.054898431473386 30-31 21.00389619528884 26.645834630260673 31.338929743979776 21.01133943047071 32-33 20.936743507806195 26.76517805637648 31.119934092666117 21.178144343151203 34-35 21.310144813777505 26.625780113937402 30.923435661365424 21.14063941091967 36-37 21.60795672345771 26.351148581599755 30.80104165221713 21.2398530427254 38-39 21.136102513996345 26.794252060037106 30.84527494001567 21.224370485950878 40-41 21.042533157714708 26.68532473600535 31.16691539957241 21.105226706707533 42-43 21.127121760668473 26.773556952385206 30.62605959047156 21.473261696474758 44-45 21.152077786310937 26.927048267975735 30.346371020945327 21.574502924767998 46-47 21.550297955955163 27.135948705699903 29.71801938286884 21.595733955476103 48-49 21.50411439865297 27.331393895269667 29.3601918101764 21.80429989590096 50-51 21.444235588972433 27.448548477024094 29.33931074987685 21.767905184126622 52-53 21.579928348396365 27.691730969170997 29.103042232763844 21.625298449668794 54-55 21.402890598989277 27.49892404429419 29.105887575486072 21.99229778123046 56-57 21.4656120047137 27.913016440776428 28.81684806568055 21.804523488829318 58-59 21.510545847989977 27.387165127331375 29.119166847390122 21.983122177288525 60-61 21.59002715352194 27.46156183160757 29.13783338847941 21.810577626391083 62-63 21.661581415114192 27.424426665800517 29.341630785700122 21.57236113338517 64-65 21.59967656646204 27.319781377874236 29.258318856073767 21.822223199589956 66-67 21.477632182694535 27.68892958512846 28.88731427828919 21.946123953887824 68-69 21.3221900238205 27.600709132003743 29.232572417635154 21.8445284265406 70-71 21.507454987096615 27.623902264658813 29.08917415640579 21.77946859183878 72-73 21.59158112689208 27.581823841068566 28.78399317937302 22.042601852666337 74-75 21.40724509496744 27.991421902840663 28.62310577735791 21.978227224833986 76-77 21.498111650983155 27.783178754119398 28.86478771343115 21.853921881466295 78-79 21.43244360853914 27.68225326261376 28.79072641419409 22.094576714653016 80-81 21.28686339784996 28.141973362981933 28.603393225263556 21.967770013904552 82-83 21.447461436707062 28.09151842743861 28.498986659230873 21.962033476623454 84-85 21.39821927935457 27.866772456518174 28.25289413627605 22.482114127851208 86-87 21.468864033765247 27.703028537142753 28.6674984796908 22.1606089494012 88-89 21.670315819543102 27.47546425584527 28.64490803559371 22.209311889017915 90-91 21.377598972747275 27.653648774468408 28.821559852396728 22.14719240038759 92-93 21.425278891433205 27.77424296061845 28.630969826725515 22.169508321222825 94-95 21.30094389130075 27.45848377161329 28.67549012343298 22.56508221365298 96-97 21.55285503291438 27.477477629893805 28.398978816706393 22.57068852048542 98-99 21.311389970079386 27.569573635474796 28.41182488673587 22.70721150770995 100-101 21.336633076768123 27.495349156077076 28.59323609021029 22.57478167694451 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1755.0 1 1206.5 2 822.0 3 973.5 4 808.0 5 836.5 6 1618.5 7 3570.5 8 6146.5 9 6771.5 10 4886.5 11 3298.0 12 3024.0 13 3098.0 14 3091.5 15 2979.5 16 2938.0 17 2942.0 18 3026.5 19 3215.0 20 3469.0 21 3936.0 22 4637.0 23 5332.0 24 5960.0 25 7335.5 26 9597.5 27 11777.0 28 13062.5 29 14655.5 30 17399.0 31 20469.0 32 24418.5 33 29311.5 34 34591.0 35 37771.5 36 41904.5 37 46531.0 38 47431.0 39 46878.5 40 45611.5 41 45743.0 42 46835.0 43 47994.5 44 49942.0 45 51603.5 46 50710.0 47 49603.5 48 55177.0 49 55120.0 50 48272.0 51 44784.5 52 42394.5 53 41995.0 54 41262.5 55 39179.0 56 36691.0 57 33461.5 58 30603.5 59 27995.0 60 24261.0 61 20143.5 62 16599.0 63 13092.5 64 9890.5 65 7475.0 66 5372.5 67 4122.5 68 2985.0 69 1852.5 70 1283.0 71 941.0 72 686.5 73 519.5 74 384.5 75 266.5 76 164.5 77 93.5 78 68.0 79 37.5 80 20.5 81 20.5 82 16.0 83 12.0 84 8.0 85 5.0 86 5.0 87 4.5 88 2.5 89 2.5 90 3.5 91 3.0 92 1.5 93 1.5 94 2.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03525212713770997 2 0.027064972850449107 3 0.02320821421926011 4 0.04607811627788961 5 0.08809648662821186 6 0.10609469357376052 7 0.05331799651538474 8 0.08112725611922121 9 0.06793308185462726 10-11 0.06783158820643809 12-13 0.06647833956391562 14-15 0.06106534499382581 16-17 0.048513963834430025 18-19 0.05737774244295211 20-21 0.05355481502782618 22-23 0.030515756888881373 24-25 0.008829947392459021 26-27 0.0029433157974863406 28-29 0.007104555373242891 30-31 0.004939357545206962 32-33 0.006258774971666357 34-35 0.006495593484107786 36-37 0.005446825786152884 38-39 0.002638834852918788 40-41 0.0068677368608014615 42-43 0.003687602550873691 44-45 0.0020298729637836833 46-47 0.0014885735067747012 48-49 3.721433766936753E-4 50-51 0.0019622105316575602 52-53 0.00537916335402676 54-55 0.011434951029314749 56-57 0.007138386589305953 58-59 0.00463487660063941 60-61 0.0021651978280359287 62-63 0.008254816719386979 64-65 0.00219902904409899 66-67 0.021043016391224183 68-69 0.042356682510952856 70-71 0.05365630867601536 72-73 0.0037214337669367525 74-75 0.014073785882233536 76-77 0.021076847607287244 78-79 0.02381717610839522 80-81 0.07243263359101443 82-83 0.026151530016746454 84-85 0.010318520899233723 86-87 0.029061014598169732 88-89 0.04340545020890776 90-91 0.03968401644197101 92-93 0.03775563712637651 94-95 0.010792157924116582 96-97 0.015393203308692932 98-99 0.03487998376101629 100-101 0.070538085491483 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1477925.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.243392509761804 #Duplication Level Percentage of deduplicated Percentage of total 1 70.64115128447384 31.960453349049146 2 14.920222087141061 13.50082928442681 3 5.800067185432941 7.872441487605959 4 2.7974730884734207 5.062686919091943 5 1.547768998892567 3.5013160165668737 6 0.9148219767015011 2.483378986107731 7 0.6262142095433388 1.9832438694291652 8 0.4373835951201448 1.583097413708116 9 0.3270240611456142 1.3316110162692634 >10 1.7237756882417687 14.41918224970956 >50 0.16692241423629622 5.232590703499109 >100 0.09328842332083723 7.578270318772915 >500 0.0022424926596123357 0.6374266413639492 >1k 0.0011959960851265791 0.8550878183492197 >5k 2.989990212816448E-4 1.0689612309121237 >10k+ 1.494995106408224E-4 0.9294226951381607 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13741 0.929749479845053 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9307 0.6297342557978246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6497 0.4396028215234197 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2013 0.13620447586988513 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1956 0.13234771723869615 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1895 0.12822030887900265 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1874 0.12679939780435406 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1551 0.10494443222761642 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 6.766243212612277E-5 5 0.0 0.0 0.0 0.0 6.766243212612277E-5 6 0.0 6.766243212612277E-5 0.0 0.0 2.029872963783683E-4 7 0.0 6.766243212612277E-5 0.0 0.0 2.029872963783683E-4 8 0.0 6.766243212612277E-5 0.0 0.0 2.029872963783683E-4 9 0.0 6.766243212612277E-5 0.0 0.0 2.029872963783683E-4 10-11 0.0 6.766243212612277E-5 0.0 0.0 2.029872963783683E-4 12-13 0.0 6.766243212612277E-5 0.0 3.3831216063061385E-5 2.029872963783683E-4 14-15 0.0 6.766243212612277E-5 0.0 1.0149364818918416E-4 2.368185124414297E-4 16-17 0.0 6.766243212612277E-5 0.0 1.3532486425224554E-4 2.706497285044911E-4 18-19 0.0 6.766243212612277E-5 0.0 1.3532486425224554E-4 2.706497285044911E-4 20-21 0.0 6.766243212612277E-5 0.0 1.6915608031530693E-4 2.706497285044911E-4 22-23 0.0 6.766243212612277E-5 0.0 4.059745927567366E-4 3.3831216063061385E-4 24-25 0.0 6.766243212612277E-5 0.0 8.457804015765346E-4 3.3831216063061385E-4 26-27 0.0 6.766243212612277E-5 0.0 0.0013870798585855167 3.3831216063061385E-4 28-29 0.0 6.766243212612277E-5 0.0 0.0023343539083512353 3.3831216063061385E-4 30-31 0.0 6.766243212612277E-5 0.0 0.005142344841585331 3.3831216063061385E-4 32-33 0.0 6.766243212612277E-5 0.0 0.008626960096080653 3.3831216063061385E-4 34-35 0.0 6.766243212612277E-5 0.0 0.011942419270260669 4.059745927567366E-4 36-37 0.0 6.766243212612277E-5 0.0 0.016949439247593755 4.059745927567366E-4 38-39 0.0 6.766243212612277E-5 0.0 0.02175347192854847 4.39805808819798E-4 40-41 0.0 6.766243212612277E-5 0.0 0.028722702437539115 4.736370248828594E-4 42-43 0.0 6.766243212612277E-5 0.0 0.04042830319535835 5.074682409459208E-4 44-45 0.0 6.766243212612277E-5 0.0 0.05456975150971802 5.412994570089822E-4 46-47 0.0 6.766243212612277E-5 0.0 0.08474719623796878 6.089618891351049E-4 48-49 0.0 6.766243212612277E-5 0.0 0.1305546627873539 6.766243212612277E-4 50-51 0.0 6.766243212612277E-5 0.0 0.1702386792293249 6.766243212612277E-4 52-53 0.0 6.766243212612277E-5 0.0 0.21097146336925082 6.766243212612277E-4 54-55 0.0 6.766243212612277E-5 0.0 0.26141380651927537 6.766243212612277E-4 56-57 0.0 6.766243212612277E-5 0.0 0.31128101899622784 6.766243212612277E-4 58-59 0.0 6.766243212612277E-5 0.0 0.37356428776832384 6.766243212612277E-4 60-61 0.0 6.766243212612277E-5 0.0 0.45269550213982446 6.766243212612277E-4 62-63 0.0 6.766243212612277E-5 0.0 0.5276993081516315 6.766243212612277E-4 64-65 0.0 6.766243212612277E-5 0.0 0.6092663700796725 7.10455537324289E-4 66-67 0.0 6.766243212612277E-5 0.0 0.7214506825447841 7.442867533873505E-4 68-69 0.0 6.766243212612277E-5 0.0 0.8492650168310301 7.442867533873505E-4 70-71 0.0 6.766243212612277E-5 0.0 0.9846913747314647 7.442867533873505E-4 72-73 0.0 6.766243212612277E-5 0.0 1.1261735203071874 0.0010149364818918417 74-75 0.0 6.766243212612277E-5 0.0 1.2700915134394506 0.0010149364818918417 76-77 0.0 6.766243212612277E-5 0.0 1.4119796336079302 0.001048767697954903 78-79 0.0 6.766243212612277E-5 0.0 1.5715614797773907 0.0010825989140179643 80-81 0.0 6.766243212612277E-5 0.0 1.7699138995551196 0.0011164301300810257 82-83 0.0 6.766243212612277E-5 0.0 1.987888424649424 0.0011502613461440872 84-85 0.0 6.766243212612277E-5 0.0 2.227548759240151 0.0011502613461440872 86-87 0.0 6.766243212612277E-5 0.0 2.5260077473484785 0.0011502613461440872 88-89 0.0 6.766243212612277E-5 0.0 2.8634402963614525 0.0011502613461440872 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6575 0.0 36.645096 1 GTATCAA 10735 0.0 35.59243 1 ATCAACG 13070 0.0 29.054873 3 TCAACGC 13075 0.0 29.043762 4 TATCAAC 13105 0.0 28.941008 2 CAACGCA 13205 0.0 28.683905 5 AACGCAG 13335 0.0 28.367672 6 ACGCAGA 14650 0.0 25.525951 7 CGCAGAG 14680 0.0 25.279575 8 GAGTACT 7690 0.0 25.242085 12-13 GTACATG 8115 0.0 24.830242 1 GCAGAGT 15190 0.0 23.709742 9 ACATGGG 8460 0.0 23.033571 3 TACATGG 8735 0.0 22.852522 2 GTACTTT 8565 0.0 22.746191 14-15 AGTACTT 8190 0.0 22.511652 12-13 CATGGGG 3350 0.0 21.564856 4 CATGGGA 5000 0.0 21.102346 4 ACTTTTT 9560 0.0 20.626251 16-17 CTTACTA 660 0.0 20.157167 9 >>END_MODULE