FastQCFastQC Report
Thu 26 May 2016
SRR936776_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936776_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477925
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119810.810663599303077No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87660.5931288800175922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65680.44440685420437437No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29200.1975743018082785No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24860.16820880626554122No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20290.13728707478390312No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.13228005480657004No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA16750.11333457381125565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76750.034.6041871
GTATCAA116650.031.1581691
ATCAACG144650.024.7925323
TATCAAC145150.024.7734172
TCAACGC145000.024.699934
CAACGCA146700.024.41375
GTACATG86200.024.141381
AACGCAG149350.023.9813276
GAGTACT83500.022.89741712-13
TACATGG91500.022.2711812
ACATGGG89450.022.0373883
CATGGGG35350.021.7679714
ACGCAGA165000.021.505217
GTATGGT2901.05501385E-1021.2980881
GTACTTT89850.021.22559514-15
AGTACTT87450.021.1570312-13
CGCAGAG167000.021.1353198
GCAGAGT173450.019.8837729
GTATAGG7250.019.6597731
TATAGGG3707.2759576E-1219.2573552
Produced by FastQC (version 0.11.5)