##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936776_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1477925 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9142716984962 31.0 31.0 34.0 30.0 34.0 2 32.05544530338143 33.0 31.0 34.0 30.0 34.0 3 32.16444068542044 33.0 31.0 34.0 30.0 34.0 4 35.9006221560634 37.0 35.0 37.0 35.0 37.0 5 35.71909129353654 37.0 35.0 37.0 33.0 37.0 6 35.81536749158448 37.0 35.0 37.0 35.0 37.0 7 35.75997428827579 37.0 35.0 37.0 33.0 37.0 8 35.71658372380195 37.0 35.0 37.0 33.0 37.0 9 37.34579494899944 39.0 37.0 39.0 34.0 39.0 10-11 37.252380195206115 39.0 37.0 39.0 34.0 39.0 12-13 37.13702319129861 39.0 37.0 39.0 33.0 39.0 14-15 38.22368624930223 40.0 37.5 41.0 33.0 41.0 16-17 38.21749716663565 40.0 37.5 41.0 33.0 41.0 18-19 38.37253717204865 40.0 38.0 41.0 33.5 41.0 20-21 38.38941861055196 40.0 38.0 41.0 34.0 41.0 22-23 38.28435576906812 40.0 38.0 41.0 34.0 41.0 24-25 38.1700343386843 40.0 37.5 41.0 33.0 41.0 26-27 37.93673900908368 40.0 37.0 41.0 33.0 41.0 28-29 37.75979464451849 40.0 37.0 41.0 33.0 41.0 30-31 37.68826530439637 39.0 37.0 41.0 33.0 41.0 32-33 37.51459478660961 40.0 36.0 41.0 33.0 41.0 34-35 37.37194106602162 39.0 36.0 41.0 32.0 41.0 36-37 37.17682967674273 39.0 36.0 41.0 32.0 41.0 38-39 36.98023242045436 39.0 35.0 41.0 31.0 41.0 40-41 36.62322648307594 39.0 35.0 40.5 30.5 41.0 42-43 36.37466177241741 39.0 35.0 40.0 30.0 41.0 44-45 36.086913409002484 39.0 35.0 40.0 30.0 41.0 46-47 35.64209516721078 38.0 35.0 40.0 28.5 41.0 48-49 35.21384238036436 38.0 35.0 40.0 27.0 41.0 50-51 34.91743221070082 38.0 34.0 40.0 26.0 41.0 52-53 34.62667320736844 38.0 34.0 40.0 25.0 41.0 54-55 34.20448195950404 38.0 33.0 40.0 24.0 41.0 56-57 33.99504710996837 37.5 33.0 40.0 23.5 41.0 58-59 33.652434325151816 37.0 33.0 40.0 23.0 41.0 60-61 33.2641625928244 36.5 32.5 39.0 22.0 40.5 62-63 32.863007595108 36.0 32.0 39.0 21.5 40.0 64-65 32.505541214878974 36.0 31.0 39.0 20.0 40.0 66-67 32.153403927804185 35.0 31.0 38.5 19.5 40.0 68-69 31.679950268112385 35.0 31.0 38.0 18.0 40.0 70-71 31.219593687095085 35.0 30.0 37.0 15.0 39.5 72-73 30.79444626757109 34.0 30.0 37.0 12.5 39.0 74-75 30.287664800311248 34.0 29.0 36.0 8.0 39.0 76-77 28.902506216485953 32.5 28.0 35.0 7.5 37.0 78-79 29.591578395385422 34.0 29.0 35.0 7.0 37.0 80-81 29.451954260195883 34.0 29.0 35.0 4.0 37.0 82-83 29.08908300488861 34.0 29.0 35.0 2.0 36.5 84-85 28.611196779268234 33.5 28.5 35.0 2.0 36.0 86-87 28.18064482297816 33.0 27.5 35.0 2.0 35.5 88-89 27.7465128474043 33.0 27.0 35.0 2.0 35.0 90-91 27.21255341103236 33.0 26.0 34.5 2.0 35.0 92-93 26.64260703350982 32.0 25.0 34.0 2.0 35.0 94-95 25.98742527530152 32.0 24.5 34.0 2.0 35.0 96-97 25.081149923033983 31.5 20.5 34.0 2.0 35.0 98-99 24.060755789366848 31.0 9.5 34.0 2.0 35.0 100-101 20.77471015105638 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.15075901439232453 1101 2 0.3302449809091712 1101 3 0.21798360884828583 1101 4 0.1249308954290953 1101 5 0.11671472090429802 1101 6 0.15992309046247755 1101 7 0.10846342149017829 1101 8 0.17702756935328523 1101 9 0.14043340902025392 1101 10-11 0.22705468281133534 1101 12-13 0.27118863084360356 1101 14-15 0.3185750080934966 1101 16-17 0.2725531410203814 1101 18-19 0.1733610489618016 1101 20-21 0.14271816195145703 1101 22-23 0.26546764470708695 1101 24-25 0.20579870444585424 1101 26-27 0.3242976633101904 1101 28-29 0.28296491296956106 1101 30-31 0.2316425811525491 1101 32-33 0.3351851054188728 1101 34-35 0.27858059844950844 1101 36-37 0.3274922859604672 1101 38-39 0.3135139289238751 1101 40-41 0.6774181797346728 1101 42-43 0.8069715313237182 1101 44-45 0.43825069486303647 1101 46-47 0.4041007258187719 1101 48-49 0.5026009845227577 1101 50-51 0.4656451103822121 1101 52-53 0.6538310469243811 1101 54-55 0.3780113857138545 1101 56-57 0.48925043549837 1101 58-59 0.6415510646298159 1101 60-61 0.6736122134174067 1101 62-63 0.5757911608129191 1101 64-65 0.5689676145736442 1101 66-67 0.6232504343884173 1101 68-69 0.37940370343979524 1101 70-71 0.5048012561993289 1101 72-73 0.7209765314738767 1101 74-75 0.4541580472209077 1101 76-77 0.4171206842257966 1101 78-79 0.6485607545137277 1101 80-81 0.5147397744706232 1101 82-83 0.8529697615146326 1101 84-85 0.5273132204933972 1101 86-87 0.24487989201301374 1101 88-89 0.4954673557018232 1101 90-91 0.5181059204858691 1101 92-93 0.3627398314501704 1101 94-95 0.5644568543348889 1101 96-97 0.45730117297451756 1101 98-99 0.4611916083930865 1101 100-101 0.5919104338995282 1102 1 -0.001361056465061239 1102 2 0.06748037202040535 1102 3 0.12749747873158412 1102 4 0.12078415910325191 1102 5 -0.04449807862949484 1102 6 0.11964984566002101 1102 7 0.14044795922200137 1102 8 0.17582391973514433 1102 9 0.2267557828787261 1102 10-11 0.2581439720998091 1102 12-13 0.17474654558872516 1102 14-15 0.22981980510016342 1102 16-17 0.14207833937066994 1102 18-19 0.23996991593809724 1102 20-21 0.23641765530211245 1102 22-23 0.10206880505800342 1102 24-25 0.16628999500605346 1102 26-27 0.26054065620402866 1102 28-29 0.12561734007999803 1102 30-31 0.1701618198033188 1102 32-33 0.045210730629065665 1102 34-35 0.3132437331397995 1102 36-37 0.2414980563983491 1102 38-39 0.4439577542966404 1102 40-41 0.48910181867791636 1102 42-43 0.48137920363691933 1102 44-45 0.5857141122203657 1102 46-47 0.446310158129144 1102 48-49 0.4007029897431096 1102 50-51 0.09292152633846484 1102 52-53 0.06690140187846794 1102 54-55 0.5021435393616329 1102 56-57 0.42775163302081154 1102 58-59 0.20731778535178336 1102 60-61 0.273272767885949 1102 62-63 0.19587223015484767 1102 64-65 0.3376469903642203 1102 66-67 0.45272126426670667 1102 68-69 0.38844843590688427 1102 70-71 -0.05330581415290325 1102 72-73 -0.10029920096189215 1102 74-75 0.13327513482454378 1102 76-77 0.2232483364418627 1102 78-79 -0.1312142555961131 1102 80-81 -0.024187193680315744 1102 82-83 0.15070828905901124 1102 84-85 0.2540827147835536 1102 86-87 0.13358485216594929 1102 88-89 0.19940120671341788 1102 90-91 0.23341010752539404 1102 92-93 0.19329210783208595 1102 94-95 -0.060001060818706264 1102 96-97 0.3942409619001239 1102 98-99 0.47125413281668926 1102 100-101 0.7455051116985949 1103 1 0.10161782673639053 1103 2 0.20960521538407306 1103 3 0.12553910395383383 1103 4 0.11752154710238472 1103 5 0.013836589449532255 1103 6 -0.0651729517067352 1103 7 0.009176953801812715 1103 8 0.15152055604139747 1103 9 0.10077642724459679 1103 10-11 0.11379526294470566 1103 12-13 0.1518073144291563 1103 14-15 0.1549099509082339 1103 16-17 0.11597306898708837 1103 18-19 0.07797160646642709 1103 20-21 0.1439729440580777 1103 22-23 0.0853287385943915 1103 24-25 0.1748011860074925 1103 26-27 0.10348068148118728 1103 28-29 0.057315486555665984 1103 30-31 0.17472967849883503 1103 32-33 0.09982078523474769 1103 34-35 0.17981817897357644 1103 36-37 0.07426765596117946 1103 38-39 0.12347577874738391 1103 40-41 0.2656556309135141 1103 42-43 0.05550806335133274 1103 44-45 0.15641043222553463 1103 46-47 -0.02816770954394343 1103 48-49 0.00643133691517761 1103 50-51 -0.18097771225080805 1103 52-53 -0.1906709805954847 1103 54-55 -0.0050751915845879125 1103 56-57 -0.1894113347178248 1103 58-59 -0.2531805840151975 1103 60-61 -0.42973229427730786 1103 62-63 -0.2418453950835584 1103 64-65 -0.31533707479110973 1103 66-67 -0.15227858094765523 1103 68-69 -0.1558505399505954 1103 70-71 -0.0738219771381381 1103 72-73 -0.04344912902022102 1103 74-75 -0.35337191220202513 1103 76-77 -0.23540068107314127 1103 78-79 -0.21847701296088218 1103 80-81 -0.16471673552829813 1103 82-83 0.07344771215275969 1103 84-85 0.03271738434314031 1103 86-87 -0.20606059451174374 1103 88-89 0.06578082454095835 1103 90-91 -0.14859108230505313 1103 92-93 -0.09171064466029577 1103 94-95 -0.0056312604383386144 1103 96-97 0.31707493882764126 1103 98-99 0.2818957798435271 1103 100-101 0.9968813578912048 1104 1 -0.02445003136734414 1104 2 -7.964617558400278E-4 1104 3 0.007018508643753307 1104 4 0.041409602808151647 1104 5 -0.1620679766011648 1104 6 0.041644824484585286 1104 7 0.13938238745809173 1104 8 0.03448503935391045 1104 9 0.02679753533966789 1104 10-11 0.10647475767167691 1104 12-13 0.08717109875578899 1104 14-15 0.16195641588835485 1104 16-17 0.11366320566759924 1104 18-19 0.2763095171272951 1104 20-21 0.18362221244041876 1104 22-23 0.0746148619664666 1104 24-25 0.15521879277998352 1104 26-27 0.1714749729188796 1104 28-29 0.13301820178551793 1104 30-31 0.10068885282176154 1104 32-33 0.062214998356182605 1104 34-35 0.2628007762495628 1104 36-37 0.0311397740429058 1104 38-39 0.028418426742469194 1104 40-41 0.18784217277141835 1104 42-43 0.27376101153208054 1104 44-45 0.37737681217657126 1104 46-47 0.2895408873963916 1104 48-49 0.40786040876101737 1104 50-51 0.40697823352451223 1104 52-53 0.2953842643292717 1104 54-55 0.3965533336457696 1104 56-57 0.24022324270737982 1104 58-59 -2.483861787467845E-4 1104 60-61 0.14562345863757997 1104 62-63 0.2801227802809194 1104 64-65 0.29544912449978256 1104 66-67 0.08296786218915031 1104 68-69 0.07604448696728738 1104 70-71 0.32510510350362765 1104 72-73 0.14710011032650527 1104 74-75 0.012846145288502697 1104 76-77 0.18018958107320415 1104 78-79 0.1753368687282979 1104 80-81 0.15002902966678633 1104 82-83 0.2733626031626386 1104 84-85 0.16095092660861 1104 86-87 -0.10065597385277059 1104 88-89 0.18670784657872375 1104 90-91 0.22559512124794523 1104 92-93 0.036251854107689496 1104 94-95 0.21500247522033078 1104 96-97 0.17109037831768248 1104 98-99 0.22201914126238265 1104 100-101 0.9239846421126074 1105 1 0.07945763532702443 1105 2 0.16277389926940344 1105 3 0.08333088491982465 1105 4 0.07907753697100617 1105 5 0.0969866056632398 1105 6 0.1312451340243257 1105 7 0.10735313104829203 1105 8 0.07577187723928347 1105 9 0.09265204621375034 1105 10-11 0.1336066581121429 1105 12-13 0.10506363996530865 1105 14-15 0.23025986019912636 1105 16-17 0.06750285400212164 1105 18-19 0.10533990297566476 1105 20-21 0.13615530880510107 1105 22-23 0.20221859933180752 1105 24-25 0.10430005104811357 1105 26-27 0.09935933285146348 1105 28-29 0.08101378893689315 1105 30-31 0.17865383341421648 1105 32-33 0.18175258250208515 1105 34-35 0.17314426726377974 1105 36-37 0.01725238002143925 1105 38-39 -0.03481599783651035 1105 40-41 0.15805578516282281 1105 42-43 0.2505545572470922 1105 44-45 0.14699552788639636 1105 46-47 -0.030126500439727977 1105 48-49 0.07139790162538873 1105 50-51 0.17944200361496598 1105 52-53 0.236268748241244 1105 54-55 0.4204776081279462 1105 56-57 0.3030010893625956 1105 58-59 -0.003257270459663175 1105 60-61 0.3348863330791616 1105 62-63 0.16736880987520664 1105 64-65 0.1649369806509995 1105 66-67 0.21088870067126209 1105 68-69 0.23020654982074973 1105 70-71 0.2163770570508703 1105 72-73 0.2325431935711677 1105 74-75 0.039628587355384326 1105 76-77 0.16548099393408933 1105 78-79 0.34510262262513436 1105 80-81 0.3736549702411516 1105 82-83 0.43814653161972217 1105 84-85 0.18910514606301732 1105 86-87 0.3313076143558362 1105 88-89 0.507424631343703 1105 90-91 0.4087353214950866 1105 92-93 0.49264006216961675 1105 94-95 0.6123804134295519 1105 96-97 0.6312943584646327 1105 98-99 0.816104507199114 1105 100-101 1.280864407995935 1106 1 0.019805902054432778 1106 2 0.1035037714315834 1106 3 0.06191106125703527 1106 4 0.04456406171397731 1106 5 0.04028639814679735 1106 6 0.09574547495476082 1106 7 0.05910075082136501 1106 8 0.15738182323767802 1106 9 0.20961004447138265 1106 10-11 0.17569825524922322 1106 12-13 0.28755959834960976 1106 14-15 0.24565331315146466 1106 16-17 0.16963037825551197 1106 18-19 0.2307466529545863 1106 20-21 0.14350842399225883 1106 22-23 0.22108680642227085 1106 24-25 0.1799072917893767 1106 26-27 0.10656675759162937 1106 28-29 0.14476888995140058 1106 30-31 0.21161944011509348 1106 32-33 0.09162788035399672 1106 34-35 6.811668823161199E-4 1106 36-37 0.06936001050313934 1106 38-39 0.16541019212903763 1106 40-41 0.2888447617946639 1106 42-43 0.29820624170968557 1106 44-45 0.286437370774415 1106 46-47 0.16548474124223134 1106 48-49 0.04403324213429016 1106 50-51 0.2968753446888712 1106 52-53 0.292666666568806 1106 54-55 0.4663342309790153 1106 56-57 0.4691830456377133 1106 58-59 0.49890053817852476 1106 60-61 0.41380433376249925 1106 62-63 0.38219122283441465 1106 64-65 0.17291833966923775 1106 66-67 0.28380418098382165 1106 68-69 0.16870872630290634 1106 70-71 0.2723347609694251 1106 72-73 0.3033554067199873 1106 74-75 0.2854270449594658 1106 76-77 0.4425853334386822 1106 78-79 0.4443067238481966 1106 80-81 0.45145810620953597 1106 82-83 0.6979090857729382 1106 84-85 0.6622476298410902 1106 86-87 0.4641222058827168 1106 88-89 0.6045311968799965 1106 90-91 0.5290236776483255 1106 92-93 0.7358338408177545 1106 94-95 0.731822359853922 1106 96-97 0.9073241384794493 1106 98-99 0.648744085303246 1106 100-101 1.4947897523652571 1107 1 0.3223844862273957 1107 2 0.35077661200673305 1107 3 0.24106723254166695 1107 4 0.12306623950575357 1107 5 0.1074617628613197 1107 6 0.10509948419069559 1107 7 0.12454437750062652 1107 8 0.20705941822088647 1107 9 0.30740404636055274 1107 10-11 0.2982269207613868 1107 12-13 0.33785461697890895 1107 14-15 0.39440321475726137 1107 16-17 0.42140803358716994 1107 18-19 0.36513592011453255 1107 20-21 0.29654093343810217 1107 22-23 0.19437933895795823 1107 24-25 0.3275945359644368 1107 26-27 0.3968026085047285 1107 28-29 0.49146497648582255 1107 30-31 0.4709480308476657 1107 32-33 0.38846844238170775 1107 34-35 0.47617854959642614 1107 36-37 0.43608013802202805 1107 38-39 0.43293584021888165 1107 40-41 0.6251397607451281 1107 42-43 0.7218211792621574 1107 44-45 0.42166954830380377 1107 46-47 0.14473094322585922 1107 48-49 0.24182363623587833 1107 50-51 0.12396392571321968 1107 52-53 0.2521315332774492 1107 54-55 0.4838918571859878 1107 56-57 0.3431097288346123 1107 58-59 0.4084713426495554 1107 60-61 0.4379305932083426 1107 62-63 0.5703242255632119 1107 64-65 0.6391281292368234 1107 66-67 0.7567695527827283 1107 68-69 0.5195012916366615 1107 70-71 0.5142697158392835 1107 72-73 0.8601395232712079 1107 74-75 0.5927777481500627 1107 76-77 0.4816634125213888 1107 78-79 0.7298967283087343 1107 80-81 0.7333616077313678 1107 82-83 0.8194898870953544 1107 84-85 0.8372737043058258 1107 86-87 1.1263619532063949 1107 88-89 0.9613069499236602 1107 90-91 0.8360365607297702 1107 92-93 1.105884612228337 1107 94-95 1.3422690367535886 1107 96-97 1.0284269079483828 1107 98-99 1.0761004625597508 1107 100-101 1.512078434407659 1108 1 0.09839260610956302 1108 2 0.1492739501083875 1108 3 0.09075267292512734 1108 4 0.04868989763436815 1108 5 -0.12596573551594759 1108 6 0.0692465532939508 1108 7 -0.040765984867057625 1108 8 -0.011589551346723681 1108 9 0.04820364567152069 1108 10-11 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-1.6287818264095506 2316 80-81 -1.5455912771681604 2316 82-83 -1.3954692958242667 2316 84-85 -1.491129104254064 2316 86-87 -1.62949511313521 2316 88-89 -2.2013287991898167 2316 90-91 -2.117140768860459 2316 92-93 -1.7913308379292658 2316 94-95 -1.9416166815777771 2316 96-97 -1.8424952317777787 2316 98-99 -2.4590211575643615 2316 100-101 -3.062407350413064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 14.0 5 52.0 6 124.0 7 286.0 8 571.0 9 1118.0 10 1950.0 11 3066.0 12 4562.0 13 6270.0 14 8031.0 15 10709.0 16 14163.0 17 18725.0 18 16242.0 19 10437.0 20 9344.0 21 9711.0 22 10750.0 23 11863.0 24 14078.0 25 16468.0 26 20267.0 27 24635.0 28 30362.0 29 37312.0 30 46106.0 31 56409.0 32 70241.0 33 89897.0 34 118760.0 35 163072.0 36 226875.0 37 282762.0 38 138079.0 39 4613.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.09860233945041 20.684861790510634 15.730516536140746 21.48601933389821 2 15.484925663587473 22.136701590661193 39.23888066387167 23.13949208187967 3 18.559369168332292 26.992493514542716 30.95684345993902 23.49129385718597 4 13.00133633303449 18.44592925892721 37.64791853443172 30.904815873606577 5 13.076827826717704 38.52515310689774 33.96513540597434 14.43288366041022 6 29.7294517651437 38.103286702640524 17.534583960620466 14.632677571595313 7 26.91309407816391 34.64098192053697 21.426667207967956 17.01925679333117 8 26.63233925943468 34.30377048903023 20.004465720520322 19.05942453101477 9 27.04137695849042 16.688747188624042 20.92638051753676 35.34349533534878 10-11 24.207588341762946 27.61023055973747 27.462320483109764 20.71986061538982 12-13 25.813080747796104 25.401769885156405 28.092968367261317 20.692180999786174 14-15 22.964373641847228 26.081303596603377 27.134264238549733 23.82005852299966 16-17 21.03858450191992 29.56848283911565 27.637803000828864 21.755129658135562 18-19 21.034501540848467 27.49158529946996 31.04096671029782 20.432946449383746 20-21 21.86192097039443 26.646162548645407 31.102205168160946 20.389711312799218 22-23 21.734385181205585 26.564256072946403 31.00513090471863 20.69622784112938 24-25 21.467554945686963 26.98702360218524 31.083100911149092 20.462320540978705 26-27 21.36943673936674 27.01334272490115 31.091265672770845 20.52595486296126 28-29 20.96088128994411 26.870790760962414 31.457154949390176 20.7111729997033 30-31 21.112911683610466 26.938951570614204 31.299592333846437 20.64854441192889 32-33 21.092730567895522 27.1112166647552 31.00837899712535 20.78767377022393 34-35 21.48968993690478 26.941658067899247 30.823113486814286 20.745538508381685 36-37 21.73120351308696 26.6747637276478 30.68825582201803 20.905776937247214 38-39 21.360912390681207 27.10588392922395 30.767911229486234 20.765292450608612 40-41 21.307137755821827 26.97102019509327 30.935643174120724 20.78619887496418 42-43 21.351489419287176 27.138521914170205 30.442647630969095 21.067341035573524 44-45 21.358373240544488 27.281308200953035 30.123671474703258 21.236647083799223 46-47 21.741001455086018 27.50711388543151 29.466895862731363 21.284988796751108 48-49 21.738792122049702 27.675436296558175 29.12947059131991 21.456300990072215 50-51 21.754254674187226 27.845414857798612 28.92599925299614 21.474331215018026 52-53 21.85312278087842 28.01068933634161 28.847269628325993 21.288918254453975 54-55 21.70018776324915 27.892552057783714 28.736031936667967 21.671228242299172 56-57 21.778539508721884 28.310149294227937 28.490436393567855 21.420874803482327 58-59 21.761086931148284 27.773107182548472 28.748124478786945 21.71768140751629 60-61 21.840557008447824 27.8567827890344 28.828874852408326 21.473785350109445 62-63 21.93420570536587 27.75872515815253 29.05686318888414 21.250205947597458 64-65 21.832982672660208 27.70205785207566 28.922925359177686 21.542034116086445 66-67 21.70634504457263 28.013228005480656 28.638327384677844 21.642099565268875 68-69 21.630585074261287 27.96365982407073 28.80626094652396 21.599494155144026 70-71 21.746411515628022 28.011424148229747 28.706386399282234 21.535777936859997 72-73 21.876639758417806 27.95641993648521 28.406409398050712 21.76053090704627 74-75 21.762176031526177 28.417134509294712 28.06998253582997 21.750706923349142 76-77 21.819205981358998 28.229815450716377 28.367609993741226 21.5833685741834 78-79 21.69666037629887 28.119838611130092 28.277830817750072 21.905670194820967 80-81 21.52817201128897 28.641663221469884 28.133781445737643 21.69638332150351 82-83 21.73245832157697 28.500571410012565 28.004875761457125 21.76209450695334 84-85 21.695935832929607 28.250575820154655 27.846622080639182 22.206866266276563 86-87 21.644511686002375 28.17459243451278 28.19001950035252 21.990876379132324 88-89 21.757867450485254 27.91379348843252 28.21337539321182 22.11496366787041 90-91 21.558622579559277 28.109607671657393 28.298827903640788 22.032941845142542 92-93 21.507417494121825 28.27937818224876 28.073007764264084 22.14019655936533 94-95 21.349256559027012 27.92418424480268 28.07185750291794 22.654701693252363 96-97 21.708133644310294 27.901825737876734 27.807470348590556 22.582570269222416 98-99 21.353105736209404 28.02796635944404 27.822068672934265 22.796859231412288 100-101 21.302973075931543 28.18041446731403 27.79185558939493 22.724756867359496 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2740.0 1 1894.0 2 1046.0 3 1062.0 4 931.0 5 914.0 6 1648.0 7 3300.0 8 5104.5 9 5646.0 10 4593.5 11 3545.5 12 3288.0 13 3221.5 14 3153.0 15 3020.0 16 3012.0 17 3129.5 18 3213.5 19 3399.5 20 3705.5 21 4078.0 22 4684.0 23 5444.0 24 6187.0 25 7488.0 26 9694.0 27 11987.5 28 13378.0 29 15007.5 30 17596.5 31 20484.0 32 24344.0 33 29523.0 34 34778.0 35 37756.5 36 41989.5 37 46268.5 38 47282.5 39 46975.0 40 45741.5 41 45932.5 42 46649.5 43 47642.0 44 49792.5 45 51633.0 46 50867.5 47 49759.0 48 54839.5 49 54717.0 50 48418.0 51 45122.5 52 42641.0 53 41860.5 54 40864.0 55 38774.0 56 36285.5 57 33223.0 58 30607.5 59 27946.5 60 24079.0 61 20073.0 62 16556.0 63 13006.0 64 9793.5 65 7409.0 66 5358.5 67 4165.5 68 2977.0 69 1715.0 70 1181.5 71 909.5 72 710.0 73 537.5 74 354.0 75 262.5 76 171.0 77 85.5 78 51.0 79 30.0 80 17.5 81 8.5 82 4.0 83 4.5 84 2.5 85 1.0 86 1.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021178341255476428 2 0.003315459174180016 3 2.029872963783683E-4 4 0.0 5 0.0024358475565404198 6 0.0 7 3.3831216063061385E-4 8 0.0 9 6.766243212612277E-5 10-11 0.0 12-13 0.0060219564592249265 14-15 0.0030109782296124632 16-17 0.0 18-19 3.721433766936753E-4 20-21 7.104555373242891E-4 22-23 0.0012179237782702099 24-25 7.442867533873506E-4 26-27 2.3681851244142973E-4 28-29 3.3831216063061385E-5 30-31 0.0 32-33 2.3681851244142973E-4 34-35 0.0 36-37 0.0015224047228377623 38-39 0.00926975320127882 40-41 0.0011164301300810259 42-43 0.0 44-45 0.0011840925622071485 46-47 3.721433766936753E-4 48-49 1.6915608031530693E-4 50-51 0.0014209110746485783 52-53 2.3681851244142973E-4 54-55 0.0 56-57 3.0448094456755247E-4 58-59 1.6915608031530693E-4 60-61 0.0020298729637836833 62-63 3.3831216063061385E-5 64-65 3.3831216063061385E-5 66-67 0.0 68-69 1.0149364818918416E-4 70-71 2.029872963783683E-4 72-73 1.0149364818918416E-4 74-75 0.0028418221492971566 76-77 0.0 78-79 2.706497285044911E-4 80-81 0.0014885735067747012 82-83 1.3532486425224554E-4 84-85 0.0022328602601620518 86-87 2.029872963783683E-4 88-89 0.0021651978280359287 90-91 0.0011840925622071485 92-93 0.0 94-95 0.0 96-97 1.3532486425224554E-4 98-99 4.39805808819798E-4 100-101 0.0018945480995314378 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1477925.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.58739963833359 #Duplication Level Percentage of deduplicated Percentage of total 1 69.82720590208031 31.134135351842616 2 15.364174987101759 13.7009722052639 3 5.8369690793299664 7.807658190500439 4 2.913968402473351 5.197050947782234 5 1.6040226897801306 3.5759600349090737 6 0.9836913556134338 2.631614375610618 7 0.6463746039061657 2.0174113946303662 8 0.4461931924044032 1.59156753485112 9 0.3332702399061104 1.337368803683135 >10 1.7831461705472278 14.853674040743247 >50 0.1623884848505552 5.013497404619006 >100 0.09372604328487673 7.457730007047743 >500 0.003347333496219983 0.9117750756926714 >1k 0.0010650606578881767 0.916523904211904 >5k 3.043030451109076E-4 1.0402648073416236 >10k+ 1.521515225554538E-4 0.8127959212703791 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11981 0.810663599303077 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8766 0.5931288800175922 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6568 0.44440685420437437 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2920 0.1975743018082785 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2486 0.16820880626554122 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2029 0.13728707478390312 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1955 0.13228005480657004 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1675 0.11333457381125565 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.3532486425224554E-4 7 0.0 0.0 0.0 0.0 1.3532486425224554E-4 8 0.0 0.0 0.0 0.0 1.3532486425224554E-4 9 0.0 0.0 0.0 0.0 1.3532486425224554E-4 10-11 0.0 0.0 0.0 0.0 1.3532486425224554E-4 12-13 0.0 0.0 0.0 3.3831216063061385E-5 1.3532486425224554E-4 14-15 0.0 0.0 0.0 1.0149364818918416E-4 2.029872963783683E-4 16-17 0.0 0.0 0.0 1.3532486425224554E-4 2.706497285044911E-4 18-19 0.0 0.0 0.0 1.3532486425224554E-4 2.706497285044911E-4 20-21 0.0 0.0 0.0 1.6915608031530693E-4 2.706497285044911E-4 22-23 0.0 0.0 0.0 4.059745927567366E-4 3.0448094456755247E-4 24-25 0.0 0.0 0.0 8.79611617639596E-4 3.3831216063061385E-4 26-27 0.0 0.0 0.0 0.0013870798585855167 3.3831216063061385E-4 28-29 0.0 0.0 0.0 0.0024696787726034815 3.3831216063061385E-4 30-31 0.0 0.0 0.0 0.005243838489774515 4.059745927567366E-4 32-33 0.0 0.0 0.0 0.00852546644789147 4.736370248828594E-4 34-35 0.0 0.0 0.0 0.011739431973882301 4.736370248828594E-4 36-37 0.0 0.0 0.0 0.01661112708696314 4.736370248828594E-4 38-39 0.0 0.0 0.0 0.02148282220004398 4.736370248828594E-4 40-41 0.0 0.0 0.0 0.028316727844782383 4.736370248828594E-4 42-43 0.0 0.0 0.0 0.03988700373834937 4.736370248828594E-4 44-45 0.0 0.0 0.0 0.054265270565150465 4.736370248828594E-4 46-47 0.0 0.0 0.0 0.0843750528612751 5.412994570089822E-4 48-49 0.0 0.0 0.0 0.12943823265727286 6.089618891351049E-4 50-51 0.0 0.0 0.0 0.16844562477798264 6.089618891351049E-4 52-53 0.0 0.0 0.0 0.2084341221645212 7.10455537324289E-4 54-55 0.0 0.0 0.0 0.2587411404502935 8.119491855134733E-4 56-57 0.0 0.0 0.0 0.3083038719826784 8.457804015765346E-4 58-59 0.0 0.0 0.0 0.37055330953871135 8.79611617639596E-4 60-61 0.0 0.0 0.0 0.449143224453203 8.79611617639596E-4 62-63 0.0 0.0 0.0 0.5234704061437488 8.79611617639596E-4 64-65 0.0 0.0 0.0 0.6046653246950962 8.79611617639596E-4 66-67 0.0 0.0 0.0 0.7166128186477663 8.79611617639596E-4 68-69 0.0 0.0 0.0 0.84429182806976 8.79611617639596E-4 70-71 0.0 0.0 0.0 0.9794475362416902 9.811052658287803E-4 72-73 0.0 0.0 0.0 1.1210650066816652 0.0012855862103963328 74-75 0.0 0.0 0.0 1.2650506622460544 0.0012855862103963328 76-77 0.0 0.0 0.0 1.4062621580932726 0.0012855862103963328 78-79 0.0 0.0 0.0 1.5653027048057242 0.0012855862103963328 80-81 0.0 0.0 0.0 1.7619297325642371 0.0013532486425224554 82-83 0.0 0.0 0.0 1.9782465280714514 0.0014209110746485783 84-85 0.0 0.0 0.0 2.216655107667845 0.0014209110746485783 86-87 0.0 0.0 0.0 2.5136931847015243 0.0014547422907116396 88-89 0.0 0.0 0.0 2.8469983253548046 0.0015562359389008238 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7675 0.0 34.604187 1 GTATCAA 11665 0.0 31.158169 1 ATCAACG 14465 0.0 24.792532 3 TATCAAC 14515 0.0 24.773417 2 TCAACGC 14500 0.0 24.69993 4 CAACGCA 14670 0.0 24.4137 5 GTACATG 8620 0.0 24.14138 1 AACGCAG 14935 0.0 23.981327 6 GAGTACT 8350 0.0 22.897417 12-13 TACATGG 9150 0.0 22.271181 2 ACATGGG 8945 0.0 22.037388 3 CATGGGG 3535 0.0 21.767971 4 ACGCAGA 16500 0.0 21.50521 7 GTATGGT 290 1.05501385E-10 21.298088 1 GTACTTT 8985 0.0 21.225595 14-15 AGTACTT 8745 0.0 21.15703 12-13 CGCAGAG 16700 0.0 21.135319 8 GCAGAGT 17345 0.0 19.883772 9 GTATAGG 725 0.0 19.659773 1 TATAGGG 370 7.2759576E-12 19.257355 2 >>END_MODULE