FastQCFastQC Report
Thu 26 May 2016
SRR936775_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936775_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219010
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107730.48548677112766503No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75960.3423148160666243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49440.2228020603782768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43450.051.9526371
GTATCAA84050.043.197581
TATCAAC103950.034.973632
TCAACGC104150.034.7263074
ATCAACG104950.034.5068783
CAACGCA105750.034.155185
AACGCAG107850.033.5341956
ACGCAGA124350.028.8552287
CGCAGAG124400.028.384558
GCAGAGT134850.025.5159059
GAGTACT76400.022.94509312-13
GTACATG79450.021.7726481
TACATGG77950.021.764862
AGAGTAC118600.021.5342110-11
GTACTTT81350.021.16957714-15
GTGGTAT21450.021.047481
CAGAGTA130350.020.94180710-11
ACATGGG78000.020.2291053
AGTACTT82650.019.9741712-13
TGGTATC23200.019.2550052
Produced by FastQC (version 0.11.5)