##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936775_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2219010 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.263390881519236 33.0 31.0 34.0 31.0 34.0 2 32.40459574314672 34.0 31.0 34.0 31.0 34.0 3 32.42870108742187 34.0 31.0 34.0 31.0 34.0 4 35.9702461908689 37.0 35.0 37.0 35.0 37.0 5 35.987785544003856 37.0 35.0 37.0 35.0 37.0 6 36.04930892605261 37.0 37.0 37.0 35.0 37.0 7 36.0084231256281 37.0 37.0 37.0 35.0 37.0 8 35.989018976931156 37.0 37.0 37.0 35.0 37.0 9 37.70588595815251 39.0 38.0 39.0 35.0 39.0 10-11 37.60732218421728 39.0 38.0 39.0 35.0 39.0 12-13 37.53019612349651 39.0 37.0 39.0 35.0 39.0 14-15 38.824263748248086 40.0 38.0 41.0 36.0 41.0 16-17 38.75123478488155 40.0 38.0 41.0 36.0 41.0 18-19 38.69726229264401 40.0 38.0 41.0 35.0 41.0 20-21 38.638676707180224 40.0 38.0 41.0 35.0 41.0 22-23 38.51064799167196 40.0 38.0 41.0 34.5 41.0 24-25 38.36195217687167 40.0 38.0 41.0 34.0 41.0 26-27 38.32726531200851 40.0 38.0 41.0 34.0 41.0 28-29 38.409138760077695 40.0 38.0 41.0 34.0 41.0 30-31 38.28710708829613 40.0 38.0 41.0 34.0 41.0 32-33 38.11393098724206 40.0 38.0 41.0 34.0 41.0 34-35 37.94398470489092 40.0 38.0 41.0 34.0 41.0 36-37 37.81073203816116 40.0 38.0 41.0 33.0 41.0 38-39 37.656668063686055 40.0 37.0 41.0 33.0 41.0 40-41 37.46722299584049 40.0 37.0 41.0 33.0 41.0 42-43 37.28064136709614 40.0 37.0 41.0 32.5 41.0 44-45 37.045196731875926 40.0 37.0 41.0 31.5 41.0 46-47 36.73828644305344 39.0 36.0 41.0 31.0 41.0 48-49 36.42342305803038 39.0 36.0 41.0 30.5 41.0 50-51 35.78797301499317 38.5 35.5 39.5 30.0 40.5 52-53 36.108064857751884 39.0 36.0 40.0 30.5 41.0 54-55 36.41594607505149 39.0 36.0 41.0 31.0 41.0 56-57 36.295438506360945 39.0 35.5 41.0 30.5 41.0 58-59 36.05523702011257 39.0 35.0 41.0 30.0 41.0 60-61 35.76543345906508 39.0 35.0 40.5 29.5 41.0 62-63 35.4586347064682 38.0 35.0 40.0 29.0 41.0 64-65 35.13513120715995 37.5 35.0 40.0 28.5 41.0 66-67 34.75506396996859 37.0 34.0 40.0 28.0 41.0 68-69 34.31972929369404 36.5 34.0 39.0 27.5 41.0 70-71 33.96315158561701 36.0 34.0 39.0 26.5 41.0 72-73 33.58074907278471 35.5 34.0 38.5 26.0 40.0 74-75 33.158376933857895 35.0 33.5 37.5 26.0 39.5 76-77 32.73815643011974 35.0 33.0 37.0 25.5 39.0 78-79 32.33538943042167 35.0 33.0 36.5 25.0 39.0 80-81 31.93689393017607 35.0 32.0 36.0 24.0 38.0 82-83 31.555433278804514 35.0 32.0 35.5 23.0 37.0 84-85 31.22950797878333 35.0 32.0 35.0 21.5 37.0 86-87 30.924703809356423 34.5 31.5 35.0 20.0 36.0 88-89 30.588310102252805 34.0 31.0 35.0 18.5 36.0 90-91 30.29130918743043 34.0 31.0 35.0 17.5 36.0 92-93 29.975869869896936 34.0 31.0 35.0 10.0 35.0 94-95 29.6307839982695 34.0 30.5 35.0 4.5 35.0 96-97 29.243475468790137 34.0 30.0 35.0 2.0 35.0 98-99 28.710439114740357 34.0 30.0 35.0 2.0 35.0 100-101 26.54163230449615 32.0 24.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2652869963270206 1101 2 0.22267788379473785 1101 3 0.29067957877178685 1101 4 0.18358556265227577 1101 5 0.15111027949044598 1101 6 0.1462254796498499 1101 7 0.06495890629599188 1101 8 0.13242633330516185 1101 9 0.17359605044382675 1101 10-11 0.18300491680356146 1101 12-13 0.26686758715006675 1101 14-15 0.3603881681904042 1101 16-17 0.4743131262569804 1101 18-19 0.4218452132879591 1101 20-21 0.4551943754720895 1101 22-23 0.48998531775301757 1101 24-25 0.44760269645800577 1101 26-27 0.4712056648624454 1101 28-29 0.4220011231481635 1101 30-31 0.36758044911129417 1101 32-33 0.4354736312440153 1101 34-35 0.4676064931000852 1101 36-37 0.5374289932312664 1101 38-39 0.4942735861681413 1101 40-41 0.5175949784697806 1101 42-43 0.5673745549374232 1101 44-45 0.5454000075708834 1101 46-47 0.498565532728783 1101 48-49 0.524514598332992 1101 50-51 0.4110156973126564 1101 52-53 0.603826748772839 1101 54-55 0.5520015794570341 1101 56-57 0.5899035107299255 1101 58-59 0.5696932846712244 1101 60-61 0.6138275997219864 1101 62-63 0.5726729565416377 1101 64-65 0.5195473748703137 1101 66-67 0.5621549120172133 1101 68-69 0.5112031141268645 1101 70-71 0.42528384179948375 1101 72-73 0.45672866730244266 1101 74-75 0.4431124558353119 1101 76-77 0.5498920471166144 1101 78-79 0.5914139067999287 1101 80-81 0.6290144659572832 1101 82-83 0.39214406607307595 1101 84-85 0.6132575623918299 1101 86-87 0.7431455252292132 1101 88-89 0.6734519417185005 1101 90-91 0.4727407481131145 1101 92-93 0.3830941793396043 1101 94-95 0.53821629973746 1101 96-97 0.4720396265256248 1101 98-99 0.5117502577274777 1101 100-101 0.6528977226834698 1102 1 0.3234429498265854 1102 2 0.3747191312495488 1102 3 0.3328245845815445 1102 4 0.22164233311783477 1102 5 0.2629069455141462 1102 6 0.2220004322365341 1102 7 0.2654907721537114 1102 8 0.22803071714856316 1102 9 0.29653961880984525 1102 10-11 0.4074059231309306 1102 12-13 0.3307833127057336 1102 14-15 0.3987821891872585 1102 16-17 0.3111036322592611 1102 18-19 0.4197721502042313 1102 20-21 0.418508498619488 1102 22-23 0.4169136598595671 1102 24-25 0.4533821708531178 1102 26-27 0.37552684996057195 1102 28-29 0.5440329110478643 1102 30-31 0.438090073217424 1102 32-33 0.4016279316930067 1102 34-35 0.32781608706893195 1102 36-37 0.3418913054539914 1102 38-39 0.2009336091736671 1102 40-41 0.40307257897786286 1102 42-43 0.3563826321666923 1102 44-45 0.36087294762445055 1102 46-47 0.11877345564739983 1102 48-49 0.1312409525465128 1102 50-51 0.05613972110432286 1102 52-53 0.2602803472104682 1102 54-55 0.26607545885001826 1102 56-57 0.32612208945183596 1102 58-59 0.2663101619867021 1102 60-61 0.33983179267161034 1102 62-63 0.3369048559906247 1102 64-65 0.44712498201880635 1102 66-67 0.11058143700865486 1102 68-69 0.13181152965259457 1102 70-71 0.24084258432323935 1102 72-73 0.2780189053772375 1102 74-75 0.379723889467499 1102 76-77 0.35119742437140644 1102 78-79 0.22701257939739605 1102 80-81 0.25350855975550246 1102 82-83 0.35421882696330087 1102 84-85 0.4565821302112951 1102 86-87 0.49452043228160036 1102 88-89 0.49079180001247025 1102 90-91 0.3891288861374882 1102 92-93 0.4644073742879229 1102 94-95 0.49541102233217416 1102 96-97 0.4317162965481778 1102 98-99 0.46406555012356776 1102 100-101 0.7791595541204757 1103 1 0.25433603001001615 1103 2 0.2829562930795362 1103 3 0.1639199243799041 1103 4 0.19434340898613556 1103 5 0.1688090706730918 1103 6 0.09558180406965988 1103 7 0.145741910154932 1103 8 0.1570907966071715 1103 9 0.1717924433285063 1103 10-11 0.21129909615243037 1103 12-13 0.03365678568482622 1103 14-15 0.18590290341821003 1103 16-17 0.30458353379702174 1103 18-19 0.3936028375709313 1103 20-21 0.28719260104314515 1103 22-23 0.25741767259528814 1103 24-25 0.461213698906505 1103 26-27 0.38931661019093866 1103 28-29 0.41794362532045426 1103 30-31 0.5038259790389574 1103 32-33 0.4757019331877217 1103 34-35 0.4346433105223326 1103 36-37 0.47627190038679856 1103 38-39 0.4515091317184883 1103 40-41 0.5402991873974159 1103 42-43 0.5251015549903357 1103 44-45 0.42338230185607273 1103 46-47 0.4520616967873039 1103 48-49 0.46405728724563033 1103 50-51 0.45226029007675805 1103 52-53 0.47455838914535065 1103 54-55 0.5081891832806633 1103 56-57 0.5431966638970707 1103 58-59 0.5332238987630902 1103 60-61 0.5145717174752207 1103 62-63 0.4946711524399703 1103 64-65 0.5258854688626258 1103 66-67 0.49547992874521185 1103 68-69 0.5466463183063652 1103 70-71 0.5360617666770437 1103 72-73 0.5087393900360553 1103 74-75 0.5453361050957923 1103 76-77 0.4151496879668528 1103 78-79 0.5431285946502769 1103 80-81 0.41981075330597406 1103 82-83 0.41361290056307 1103 84-85 0.49388876553674166 1103 86-87 0.48743154754606977 1103 88-89 0.44378542358030515 1103 90-91 0.5470986179873414 1103 92-93 0.5706282720639031 1103 94-95 0.6284883936773511 1103 96-97 0.45587911884508614 1103 98-99 0.5674507837754312 1103 100-101 0.724708189143648 1104 1 0.40909870018434447 1104 2 0.3950011448193891 1104 3 0.3716979752052012 1104 4 0.33143496328753486 1104 5 0.351077777047081 1104 6 0.30912858375936736 1104 7 0.32445306430985 1104 8 0.3140293194923771 1104 9 0.30612072567804915 1104 10-11 0.4325421733601331 1104 12-13 0.4289346617964185 1104 14-15 0.5001644464032395 1104 16-17 0.44979832237818584 1104 18-19 0.5589086856642851 1104 20-21 0.5140346489266463 1104 22-23 0.557964316834223 1104 24-25 0.46778032937672265 1104 26-27 0.4477871244896434 1104 28-29 0.44277139572707824 1104 30-31 0.46126476761606483 1104 32-33 0.35410055946786656 1104 34-35 0.43508651154169087 1104 36-37 0.4017775980865963 1104 38-39 0.39015990735839523 1104 40-41 0.362769034353299 1104 42-43 0.5086289751309252 1104 44-45 0.463702857666739 1104 46-47 0.44498299633198 1104 48-49 0.3129306316268057 1104 50-51 0.38603032719738195 1104 52-53 0.5525631027305096 1104 54-55 0.5100830798384592 1104 56-57 0.44155502851580053 1104 58-59 0.47459468088836587 1104 60-61 0.5127847457046357 1104 62-63 0.4865566856115606 1104 64-65 0.5589979725443186 1104 66-67 0.4479456664347552 1104 68-69 0.4859572576206048 1104 70-71 0.3612711880532089 1104 72-73 0.4059936275740128 1104 74-75 0.43793673193044214 1104 76-77 0.4840978784284289 1104 78-79 0.5366745433380729 1104 80-81 0.6408524967937268 1104 82-83 0.5308258472170664 1104 84-85 0.6697588207259422 1104 86-87 0.5057689897221813 1104 88-89 0.4726260818863004 1104 90-91 0.3878330356214512 1104 92-93 0.26873182150940167 1104 94-95 0.28322882756199874 1104 96-97 0.24415857386030737 1104 98-99 0.2709392637255412 1104 100-101 0.6203146540843889 1105 1 0.25810057977665934 1105 2 0.3101765767203304 1105 3 0.2949706177764213 1105 4 0.21789994108438293 1105 5 0.23764614752974467 1105 6 0.19753463770985036 1105 7 0.20808387385881133 1105 8 0.1907006320421658 1105 9 0.22701467959696942 1105 10-11 0.2642888384205122 1105 12-13 0.22571666626661369 1105 14-15 0.2371391685337585 1105 16-17 0.34906606043130495 1105 18-19 0.27892483962191505 1105 20-21 0.34957189742262784 1105 22-23 0.3131533618864992 1105 24-25 0.3872837370656441 1105 26-27 0.26027325614098373 1105 28-29 0.35417220634150226 1105 30-31 0.19975011337788828 1105 32-33 0.18740343553182726 1105 34-35 0.13646465590441892 1105 36-37 0.24462400004083662 1105 38-39 0.25513687653909756 1105 40-41 0.4006548339131015 1105 42-43 0.40868082912468395 1105 44-45 0.3475854487689034 1105 46-47 0.29873916589966143 1105 48-49 0.4940974108605545 1105 50-51 0.34856131708578886 1105 52-53 0.31955717518972904 1105 54-55 0.3973487269157374 1105 56-57 0.47989814693116983 1105 58-59 0.4941637501584495 1105 60-61 0.39094539902029624 1105 62-63 0.2657967480570633 1105 64-65 0.32567148826480263 1105 66-67 0.48693364253541205 1105 68-69 0.5648678823291959 1105 70-71 0.5765925652332768 1105 72-73 0.47749696116703433 1105 74-75 0.44814357379363656 1105 76-77 0.2492871113479751 1105 78-79 0.37097456106064897 1105 80-81 0.4307234162148248 1105 82-83 0.4875968660501684 1105 84-85 0.5381316550137463 1105 86-87 0.550827837502009 1105 88-89 0.5593081054979301 1105 90-91 0.5191565263739832 1105 92-93 0.4110272862752957 1105 94-95 0.5154128686447592 1105 96-97 0.4715786031649749 1105 98-99 0.6478711600012623 1105 100-101 0.7810758948859551 1106 1 0.2949273696619912 1106 2 0.33640657100804816 1106 3 0.31686523558391855 1106 4 0.22852144630756044 1106 5 0.2910003338490412 1106 6 0.31107972768922565 1106 7 0.3072502873729448 1106 8 0.24185435352979567 1106 9 0.4208006448203463 1106 10-11 0.40798265127562416 1106 12-13 0.4347505053720653 1106 14-15 0.5122410230638366 1106 16-17 0.5290800431723639 1106 18-19 0.48962650577743005 1106 20-21 0.6371414295822007 1106 22-23 0.6107568915838328 1106 24-25 0.5447204622553627 1106 26-27 0.5587492536467593 1106 28-29 0.5567095215098661 1106 30-31 0.5189593762897573 1106 32-33 0.6122148792861779 1106 34-35 0.49506316954776963 1106 36-37 0.42221375048815446 1106 38-39 0.540351363356514 1106 40-41 0.44473128258757555 1106 42-43 0.46642279086547234 1106 44-45 0.5783367958068695 1106 46-47 0.3881590856369854 1106 48-49 0.4238853005277221 1106 50-51 0.3220353886942817 1106 52-53 0.37683059882463255 1106 54-55 0.3290428188442647 1106 56-57 0.28356251172663605 1106 58-59 0.3604132359802108 1106 60-61 0.38027854436667496 1106 62-63 0.41148477304576403 1106 64-65 0.349327902374867 1106 66-67 0.3949602010223714 1106 68-69 0.382626181492995 1106 70-71 0.4561395360512037 1106 72-73 0.4795383396153028 1106 74-75 0.4286388855055563 1106 76-77 0.4414899163265247 1106 78-79 0.5289605666915449 1106 80-81 0.5561486761382852 1106 82-83 0.5947313343789702 1106 84-85 0.6261228496660003 1106 86-87 0.7932544099426373 1106 88-89 0.8013609085142477 1106 90-91 0.757146921597176 1106 92-93 0.8621028817409275 1106 94-95 0.8265410665426991 1106 96-97 0.7807086644593859 1106 98-99 0.8708781470112683 1106 100-101 0.8528526332444493 1107 1 0.15017617834881491 1107 2 0.2731880724532445 1107 3 0.282130449239105 1107 4 0.21606148224225308 1107 5 0.18162294145782454 1107 6 0.2124343093625356 1107 7 0.2799680437982275 1107 8 0.18962874615537118 1107 9 0.1346573747633215 1107 10-11 0.2615628403233501 1107 12-13 0.22499501751956075 1107 14-15 0.20142077464574015 1107 16-17 0.3092120641082303 1107 18-19 0.34526044062626937 1107 20-21 0.3851634591111477 1107 22-23 0.39305593072139544 1107 24-25 0.3676491397024364 1107 26-27 0.3225317706376174 1107 28-29 0.2461642516090805 1107 30-31 0.419431995616371 1107 32-33 0.3784565791533794 1107 34-35 0.49437181730966273 1107 36-37 0.4503498336066585 1107 38-39 0.4397810954086978 1107 40-41 0.538741906757572 1107 42-43 0.5258900199881893 1107 44-45 0.5249167504979084 1107 46-47 0.47521790916639617 1107 48-49 0.4880802852945365 1107 50-51 0.4182255300894866 1107 52-53 0.32380036681150415 1107 54-55 0.47110047059022264 1107 56-57 0.45767547165164757 1107 58-59 0.40711115020565813 1107 60-61 0.48201356506817916 1107 62-63 0.49895997029597794 1107 64-65 0.5059492600812945 1107 66-67 0.48715690440491954 1107 68-69 0.6288030426757132 1107 70-71 0.6155028670409664 1107 72-73 0.474554353989177 1107 74-75 0.4916488066054754 1107 76-77 0.40621767197061587 1107 78-79 0.5207955795811614 1107 80-81 0.5262197643345203 1107 82-83 0.5840298266842581 1107 84-85 0.6547768610713582 1107 86-87 0.627522045283623 1107 88-89 0.5335701109203121 1107 90-91 0.4727011751711139 1107 92-93 0.5950034363851024 1107 94-95 0.6084252943374011 1107 96-97 0.5400227805440636 1107 98-99 0.6100641481049323 1107 100-101 0.5895114880214223 1108 1 -0.08320262244686205 1108 2 0.045075919379456764 1108 3 -0.048443577473030075 1108 4 0.0499394778849549 1108 5 -0.07999683688829862 1108 6 -0.013878089993340836 1108 7 -0.01138082425141107 1108 8 -0.10705307633207184 1108 9 -0.21833684708000334 1108 10-11 -0.11794696359822154 1108 12-13 -0.17597021881652353 1108 14-15 0.057615242771149155 1108 16-17 -0.023071255016525072 1108 18-19 0.0838945222782499 1108 20-21 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-1.630713590995441 2316 80-81 -1.5323402160188593 2316 82-83 -1.5526760039670542 2316 84-85 -1.3982386364922021 2316 86-87 -1.2297481086579793 2316 88-89 -1.491882859984596 2316 90-91 -1.3623191823456544 2316 92-93 -1.6583509653624162 2316 94-95 -2.0529109342872047 2316 96-97 -2.0000467581481445 2316 98-99 -1.7419404533401277 2316 100-101 -1.6350382554092384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5167.0 3 1355.0 4 1275.0 5 1267.0 6 1264.0 7 1466.0 8 1807.0 9 2083.0 10 2609.0 11 3345.0 12 4511.0 13 5911.0 14 6563.0 15 8256.0 16 11156.0 17 16454.0 18 14553.0 19 9053.0 20 8218.0 21 8324.0 22 8731.0 23 9424.0 24 10698.0 25 12388.0 26 14476.0 27 17238.0 28 21087.0 29 26283.0 30 33959.0 31 44822.0 32 60920.0 33 86358.0 34 131481.0 35 210022.0 36 345434.0 37 528177.0 38 459900.0 39 82933.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.763656235120976 18.678851231441804 14.817565179125367 23.73992735431185 2 15.182166422130322 19.736045078279442 40.510463795164995 24.571324704425244 3 18.67199428578128 24.17382371959925 29.940531000267857 27.213650994351617 4 12.985295630376758 16.761952714298864 37.131014706174284 33.12173694915009 5 14.22996146018274 36.41579699476032 34.19393863941454 15.160302905642403 6 31.691221144560572 35.728580816880864 17.765104240197417 14.81509379836115 7 27.989464695950744 32.4505949000576 21.175482999665753 18.3844574043259 8 26.135987436100283 34.149668086810706 20.090197037091826 19.624147439997188 9 25.93608253468455 16.62720225193018 20.518458203733818 36.91825700965145 10-11 24.49489407779785 26.234202788460305 27.662354387561113 21.608548746180738 12-13 25.57147460710451 23.666841134039426 28.06792907078196 22.6937551880741 14-15 22.82653945327528 24.84686539173639 26.368040837699642 25.958554317288684 16-17 22.082756800828175 27.969624489860358 26.87783825965558 23.069780449655887 18-19 22.09778171842296 26.670295628063627 29.226481015375583 22.005441638137828 20-21 22.822467842723118 25.80590469199087 29.312249570950726 22.059377894335285 22-23 22.493395350263455 25.688677205396093 29.272380332224536 22.545547112115916 24-25 22.329128112799918 25.864417088981007 29.26213969141576 22.544315106803314 26-27 22.128282722626395 26.027490829476964 29.215960858007477 22.62826558988916 28-29 22.144617162609816 26.173569543905757 29.21465628629165 22.467157007192778 30-31 22.307188232687196 25.921761490192065 29.102445294380065 22.668604982740675 32-33 22.109855419226086 25.928801345876877 29.20729105308824 22.754052181808795 34-35 22.38786658545745 26.043904372088495 28.98492835660885 22.583300685845206 36-37 22.40254909740131 26.006564354114442 28.963431678418782 22.627454870065463 38-39 22.2158952707691 26.10467107103641 29.034440331926 22.64499332626849 40-41 22.462285217654003 25.975455378590695 28.947728546001155 22.614530857754144 42-43 22.271365914857594 26.095593863037664 28.80898248208587 22.82405774001887 44-45 22.341207779801028 26.20180397393629 28.702612096913445 22.75437614934924 46-47 22.511494011934207 26.357519556386812 28.38505920447328 22.745927227205705 48-49 22.62035981377719 26.42093622630571 28.10506973437508 22.853634225542027 50-51 22.452225459366034 26.616624284798938 28.08508106419619 22.846069191638836 52-53 22.569349508630385 26.68552096629826 27.82561478398288 22.91951474108848 54-55 22.472468331960176 26.60802861873359 27.876756408276805 23.042746641029428 56-57 22.445901391007258 26.81939357980697 27.859662142087156 22.875042887098616 58-59 22.593182806495413 26.642975285233245 27.863079926175587 22.900761982095755 60-61 22.48861759422256 26.559525411670343 28.091396485988657 22.86046050811844 62-63 22.373479803073828 26.638688084649942 28.15691241258008 22.830919699696153 64-65 22.388740285392345 26.58881832007247 28.01388665676845 23.008554737766733 66-67 22.321976684914947 26.729316366933602 27.818779985612093 23.12992696253936 68-69 22.27335939435514 26.67284190100133 28.056250688965438 22.997548015678092 70-71 22.37511882873742 26.539882958482853 27.9152086810675 23.16978953171223 72-73 22.401343707871092 26.676198044114475 27.78406291790861 23.138395330105826 74-75 22.366073813198078 26.955874401581465 27.567742634896458 23.110309150324 76-77 22.505134895832395 26.75172530696669 27.553494884940466 23.189644912260455 78-79 22.42400850741299 26.794518336725915 27.612863111044778 23.168610044816322 80-81 22.46787130715853 26.78248736885644 27.57156333153385 23.17807799245118 82-83 22.50292461999103 26.860580225668617 27.53187322896759 23.10462192537276 84-85 22.50759949344977 26.81079645486984 27.46393692876981 23.217667122910576 86-87 22.356484774563437 26.8783148705728 27.57088538960051 23.194314965263256 88-89 22.455238422108575 26.74058771677223 27.481587200147207 23.32258666097199 90-91 22.460578804290236 26.833715171800254 27.489303487858802 23.21640253605071 92-93 22.436554197808224 26.852033900292305 27.487867306528518 23.223544595370953 94-95 22.408914067083693 26.78102982765253 27.493811554680875 23.3162445505829 96-97 22.44501387662064 26.831388065722887 27.388099098036573 23.3354989596199 98-99 22.390411717508115 26.8260728631331 27.258360343781785 23.525155075576997 100-101 22.537249905094704 26.663414059787964 27.271326736541322 23.528009298576002 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 805.0 1 588.5 2 438.5 3 519.5 4 438.0 5 437.5 6 859.5 7 1850.0 8 3267.5 9 3906.5 10 3125.0 11 2324.5 12 2275.0 13 2403.0 14 2490.0 15 2598.0 16 2713.5 17 2860.5 18 3120.0 19 3363.5 20 3672.5 21 4273.5 22 5228.0 23 6436.0 24 7937.5 25 10018.5 26 12879.5 27 16165.5 28 19172.5 29 22320.0 30 25501.5 31 28833.0 32 32835.5 33 36426.5 34 39886.5 35 43136.0 36 47795.0 37 52527.0 38 54773.5 39 56222.5 40 58828.0 41 62478.0 42 65373.5 43 68763.0 44 72881.0 45 76289.0 46 78431.0 47 80435.0 48 83136.5 49 83515.0 50 81945.0 51 81755.0 52 82744.5 53 82786.5 54 80777.5 55 76030.5 56 69708.5 57 63674.5 58 58570.5 59 51952.0 60 43619.5 61 35798.0 62 28561.0 63 22817.5 64 17146.5 65 11937.0 66 8752.0 67 6853.5 68 5150.5 69 3738.5 70 2736.5 71 1900.5 72 1263.5 73 941.5 74 635.0 75 344.5 76 221.0 77 119.0 78 76.5 79 56.5 80 43.5 81 33.0 82 29.0 83 25.5 84 19.0 85 16.0 86 14.0 87 10.0 88 8.5 89 8.0 90 8.0 91 6.0 92 4.5 93 5.0 94 4.5 95 4.5 96 3.5 97 1.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0505630889450701 2 0.08512805260003335 3 0.062370156060585574 4 0.11383454783890114 5 0.093825624940852 6 0.08864313364969063 7 0.09387069008251428 8 0.08400142405847652 9 0.10117124303180246 10-11 0.09569582831983632 12-13 0.08046381043798811 14-15 0.0830550560835688 16-17 0.09429880892830586 18-19 0.0749433305843597 20-21 0.059305726427551025 22-23 0.0650515319894908 24-25 0.05279381345735261 26-27 0.04659735647879009 28-29 0.058562151590123526 30-31 0.042068309741731674 32-33 0.044006110833209405 34-35 0.03925173838783962 36-37 0.04371318741240463 38-39 0.04441169710816986 40-41 0.05464148426550579 42-43 0.05126159864083532 44-45 0.04087408348768144 46-47 0.03068936147200779 48-49 0.036367569321454164 50-51 0.022104451985344817 52-53 0.034767756792443476 54-55 0.03976998751695576 56-57 0.04402864340404054 58-59 0.047070540466243954 60-61 0.04522286965809077 62-63 0.050608154086732374 64-65 0.03449736594246984 66-67 0.0529740740240017 68-69 0.04553832564972668 70-71 0.021676333139553226 72-73 0.01680929784002776 74-75 0.02516888161837937 76-77 0.03848563097958098 78-79 0.05315433459065078 80-81 0.03526347335072848 82-83 0.05410070256555851 84-85 0.0569398064902817 86-87 0.056669415640308064 88-89 0.0781654882132122 90-91 0.05547518938625784 92-93 0.06525432512697103 94-95 0.0707973375514306 96-97 0.08073420128796174 98-99 0.1094857616684918 100-101 0.1048440520772777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2219010.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.81811465470478 #Duplication Level Percentage of deduplicated Percentage of total 1 74.7005697226669 43.19046104996923 2 14.322376990602626 16.561856699411365 3 4.800867223877262 8.327312747764493 4 2.1623384198455384 5.0008932272361 5 1.1460073628554102 3.312999255035499 6 0.6944823116630477 2.409219475283911 7 0.4579780319392182 1.8535598451998436 8 0.31831997127604844 1.472372847689672 9 0.2230823512523095 1.1608380865932437 >10 1.097219102403795 11.087775176112736 >50 0.05541648045396298 2.183567257136888 >100 0.02032701633111456 2.0181697927553857 >500 6.246245125015439E-4 0.2244523115908875 >1k 2.3423419218794188E-4 0.36728560078491057 >5k 7.807806406264728E-5 0.34290823790095626 >10k+ 7.807806406264728E-5 0.4863283895348871 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10773 0.48548677112766503 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7596 0.3423148160666243 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4944 0.2228020603782768 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.506514166227282E-5 0.0 10-11 0.0 0.0 0.0 9.013028332454563E-5 0.0 12-13 0.0 0.0 0.0 9.013028332454563E-5 4.506514166227282E-5 14-15 0.0 0.0 0.0 9.013028332454563E-5 4.506514166227282E-5 16-17 0.0 0.0 0.0 9.013028332454563E-5 4.506514166227282E-5 18-19 0.0 0.0 0.0 9.013028332454563E-5 4.506514166227282E-5 20-21 0.0 0.0 0.0 9.013028332454563E-5 9.013028332454563E-5 22-23 0.0 0.0 0.0 1.5772799581795487E-4 1.3519542498681845E-4 24-25 0.0 0.0 0.0 2.2532570831136409E-4 1.3519542498681845E-4 26-27 0.0 0.0 0.0 2.929234208047733E-4 1.3519542498681845E-4 28-29 0.0 0.0 0.0 7.886399790897743E-4 1.3519542498681845E-4 30-31 0.0 0.0 0.0 0.002118061658126822 1.3519542498681845E-4 32-33 0.0 0.0 0.0 0.004506514166227282 1.8026056664909127E-4 34-35 0.0 0.0 0.0 0.00752587865759956 1.8026056664909127E-4 36-37 0.0 4.506514166227282E-5 0.0 0.012212653390475933 1.8026056664909127E-4 38-39 0.0 9.013028332454563E-5 0.0 0.019040022352310264 2.2532570831136409E-4 40-41 0.0 9.013028332454563E-5 0.0 0.02739960613066187 2.2532570831136409E-4 42-43 0.0 9.013028332454563E-5 0.0 0.03720127444220621 2.2532570831136409E-4 44-45 0.0 9.013028332454563E-5 0.0 0.05186997805327601 2.2532570831136409E-4 46-47 0.0 9.013028332454563E-5 0.0 0.07068467469727491 2.2532570831136409E-4 48-49 0.0 9.013028332454563E-5 0.0 0.089364175916287 2.929234208047733E-4 50-51 0.0 9.013028332454563E-5 0.0 0.11056732506838635 3.6052113329818254E-4 52-53 0.0 9.013028332454563E-5 0.0 0.13222112563710842 4.5065141662272817E-4 54-55 0.0 9.013028332454563E-5 0.0 0.15660136727639803 6.534445541029558E-4 56-57 0.0 9.013028332454563E-5 0.0 0.19067061437307628 6.759771249340923E-4 58-59 0.0 9.013028332454563E-5 0.0 0.22688045569871249 6.759771249340923E-4 60-61 0.0 9.013028332454563E-5 0.0 0.26653778036151254 7.210422665963651E-4 62-63 0.0 9.013028332454563E-5 0.0 0.31070161919053996 7.661074082586379E-4 64-65 0.0 9.013028332454563E-5 0.0 0.3591466464774832 7.661074082586379E-4 66-67 0.0 9.013028332454563E-5 0.0 0.4139233261679758 7.661074082586379E-4 68-69 0.0 9.013028332454563E-5 0.0 0.4758428308119387 7.661074082586379E-4 70-71 0.0 9.013028332454563E-5 0.0 0.5516649316587127 8.562376915831834E-4 72-73 0.0 9.013028332454563E-5 0.0 0.6301458758635607 8.7877026241432E-4 74-75 0.0 9.013028332454563E-5 0.0 0.7181806301008107 9.013028332454563E-4 76-77 0.0 9.013028332454563E-5 0.0 0.8207714250949747 9.013028332454563E-4 78-79 0.0 9.013028332454563E-5 0.0 0.9329385626923719 9.013028332454563E-4 80-81 0.0 9.013028332454563E-5 0.0 1.0582647216551524 9.463679749077292E-4 82-83 0.0 9.013028332454563E-5 0.0 1.1919279318254536 9.91433116570002E-4 84-85 0.0 9.013028332454563E-5 0.0 1.332057989824291 9.91433116570002E-4 86-87 0.0 9.013028332454563E-5 0.0 1.4816742601430368 0.001059030829063411 88-89 0.0 9.013028332454563E-5 0.0 1.6534625801596206 0.001216758824881366 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4345 0.0 51.952637 1 GTATCAA 8405 0.0 43.19758 1 TATCAAC 10395 0.0 34.97363 2 TCAACGC 10415 0.0 34.726307 4 ATCAACG 10495 0.0 34.506878 3 CAACGCA 10575 0.0 34.15518 5 AACGCAG 10785 0.0 33.534195 6 ACGCAGA 12435 0.0 28.855228 7 CGCAGAG 12440 0.0 28.38455 8 GCAGAGT 13485 0.0 25.515905 9 GAGTACT 7640 0.0 22.945093 12-13 GTACATG 7945 0.0 21.772648 1 TACATGG 7795 0.0 21.76486 2 AGAGTAC 11860 0.0 21.53421 10-11 GTACTTT 8135 0.0 21.169577 14-15 GTGGTAT 2145 0.0 21.04748 1 CAGAGTA 13035 0.0 20.941807 10-11 ACATGGG 7800 0.0 20.229105 3 AGTACTT 8265 0.0 19.97417 12-13 TGGTATC 2320 0.0 19.255005 2 >>END_MODULE