FastQCFastQC Report
Thu 26 May 2016
SRR936775_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936775_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2219010
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95050.4283441714999031No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81910.3691285753556766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53100.23929590222666863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61750.046.3904571
GTATCAA96850.035.5129931
TATCAAC123650.027.6200452
ATCAACG124200.027.458873
TCAACGC125250.027.1907524
CAACGCA127400.026.69465
AACGCAG131400.025.9181276
ACCGCGT1000.001654434823.7492458
ACGCAGA151250.022.359627
CGCAGAG153600.021.8010648
GAGTACT93300.021.66171612-13
GTACTTT94750.021.08027814-15
GCAGAGT160000.020.2462339
AGAGTAC135600.019.73849510-11
AGTACTT95000.019.39916412-13
TATTCCG2502.1750384E-718.9993975
CAGAGTA156350.018.5467410-11
TTACGCG800.00119190817.81213680-81
GTACATG76100.017.3543531
ACTTTTT117250.017.29834716-17
Produced by FastQC (version 0.11.5)