##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936775_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2219010 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.275520615049054 31.0 31.0 33.0 30.0 34.0 2 31.292109093694936 31.0 31.0 34.0 28.0 34.0 3 31.447567158327363 31.0 31.0 34.0 30.0 34.0 4 35.33552169661245 37.0 35.0 37.0 33.0 37.0 5 35.19061878946017 37.0 35.0 37.0 32.0 37.0 6 35.264051536496005 37.0 35.0 37.0 32.0 37.0 7 35.18397573692773 37.0 35.0 37.0 32.0 37.0 8 35.159847409430334 37.0 35.0 37.0 32.0 37.0 9 36.69450070076295 39.0 35.0 39.0 32.0 39.0 10-11 36.54540087696766 38.0 35.0 39.0 32.0 39.0 12-13 36.40816512769208 38.0 35.0 39.0 32.0 39.0 14-15 37.323657847418445 39.0 36.0 40.0 32.0 41.0 16-17 37.16574823907959 39.0 36.0 40.0 32.0 41.0 18-19 37.20221269845561 38.5 36.0 40.0 31.5 41.0 20-21 37.308434617239215 39.0 36.0 40.0 32.0 41.0 22-23 37.194025038192706 39.0 36.0 40.0 31.0 41.0 24-25 37.03973416072934 39.0 36.0 40.0 31.0 41.0 26-27 36.76648843403139 39.0 36.0 40.0 30.5 41.0 28-29 36.5005829176074 38.0 35.0 40.0 30.0 41.0 30-31 36.298070535959724 38.0 35.0 40.0 30.0 41.0 32-33 36.2310712885476 38.0 35.0 40.0 30.0 41.0 34-35 36.061914998129794 38.0 35.0 40.0 30.0 41.0 36-37 35.83152892506118 38.0 34.5 40.0 29.0 41.0 38-39 35.58342481557091 38.0 34.0 40.0 28.5 41.0 40-41 35.306826242333294 38.0 34.0 40.0 27.0 41.0 42-43 34.98374162351679 38.0 33.5 40.0 26.5 41.0 44-45 34.534656671218244 38.0 33.0 40.0 24.5 41.0 46-47 34.194064244865956 38.0 33.0 40.0 24.0 41.0 48-49 33.767481444427915 37.0 32.5 40.0 23.0 41.0 50-51 33.38125943551404 37.0 32.0 40.0 22.5 41.0 52-53 33.007417046340485 37.0 31.0 39.0 20.5 40.0 54-55 32.65090828793021 36.0 31.0 39.0 19.0 40.0 56-57 32.24560254347659 36.0 30.5 39.0 18.0 40.0 58-59 31.745821334739365 35.0 30.0 39.0 14.5 40.0 60-61 31.24412868801853 35.0 29.0 38.0 12.5 40.0 62-63 30.722660555833457 34.5 28.5 38.0 8.0 40.0 64-65 30.185813493404716 34.0 28.0 38.0 8.0 40.0 66-67 29.665409799865706 34.0 27.5 37.0 7.0 39.0 68-69 29.070376429128302 33.5 26.0 36.5 4.0 39.0 70-71 28.44796756211103 33.0 26.0 36.0 2.0 39.0 72-73 27.908748045299482 32.0 26.0 35.5 2.0 38.0 74-75 27.305523409087836 32.0 25.0 35.0 2.0 37.5 76-77 26.346849946597807 30.5 24.0 34.0 2.0 36.0 78-79 27.024397366393124 32.0 25.0 35.0 2.0 36.0 80-81 26.75840487424572 32.0 25.0 35.0 2.0 36.0 82-83 26.252633156227326 31.5 23.5 34.5 2.0 36.0 84-85 25.679208070265567 31.0 21.5 34.0 2.0 35.5 86-87 25.073893087457922 31.0 20.0 34.0 2.0 35.0 88-89 24.430946007453773 31.0 18.0 34.0 2.0 35.0 90-91 23.66261891564256 30.0 10.0 34.0 2.0 35.0 92-93 22.790828342368894 29.5 4.5 34.0 2.0 35.0 94-95 21.86425635756486 29.0 2.0 34.0 2.0 35.0 96-97 20.69805296055448 29.0 2.0 33.5 2.0 35.0 98-99 19.355407591673767 26.5 2.0 33.0 2.0 34.0 100-101 16.312125227015652 19.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2395810987407998 1101 2 0.21874385774090754 1101 3 0.24759515510157826 1101 4 0.291461005491513 1101 5 0.22638698192518802 1101 6 0.33165636452938685 1101 7 0.3614117922433451 1101 8 0.2401728809590864 1101 9 0.5439247365375479 1101 10-11 0.4334162398120611 1101 12-13 0.39616003138542766 1101 14-15 0.4919622451929371 1101 16-17 0.4735066771020797 1101 18-19 0.5029919442556263 1101 20-21 0.5089554151023421 1101 22-23 0.38575353933923395 1101 24-25 0.5189714996950485 1101 26-27 0.5472331179311922 1101 28-29 0.5619610792653091 1101 30-31 0.49020089468906036 1101 32-33 0.7397679374142925 1101 34-35 0.7689533290335788 1101 36-37 0.7743267974693993 1101 38-39 0.815474721243497 1101 40-41 0.6773099412718651 1101 42-43 0.8002119742427425 1101 44-45 1.0103101288506977 1101 46-47 0.7904591768060527 1101 48-49 0.7597544589679046 1101 50-51 0.8582763067373804 1101 52-53 0.678347796935654 1101 54-55 0.7953237550716565 1101 56-57 0.785266934666744 1101 58-59 0.8170807062663563 1101 60-61 0.9540777512254586 1101 62-63 0.9840305079349214 1101 64-65 0.7002616751923938 1101 66-67 0.7430728057548706 1101 68-69 0.46959296575438003 1101 70-71 0.5158611014912253 1101 72-73 0.6984443086250955 1101 74-75 0.705843770425961 1101 76-77 0.49058992431350035 1101 78-79 0.8228254795498771 1101 80-81 1.2402134689680864 1101 82-83 1.3196411029924562 1101 84-85 1.2662481886658377 1101 86-87 1.0782130282029279 1101 88-89 1.4261327707876852 1101 90-91 1.4228380910722933 1101 92-93 1.394202964356598 1101 94-95 1.2472945897426087 1101 96-97 1.1295561486826031 1101 98-99 1.0383482442046024 1101 100-101 0.9006569759878182 1102 1 0.344131439068299 1102 2 0.2655055350861595 1102 3 0.37338279258924345 1102 4 0.41585889836284196 1102 5 0.3516579803010771 1102 6 0.388229198603824 1102 7 0.4216109512696846 1102 8 0.3093381852860304 1102 9 0.47663139765513307 1102 10-11 0.4773741648590146 1102 12-13 0.5610664197350701 1102 14-15 0.7566932097537773 1102 16-17 0.663315666573979 1102 18-19 0.7242281103555825 1102 20-21 0.6209874146197549 1102 22-23 0.6898781753628427 1102 24-25 0.6288915787379139 1102 26-27 0.6009339712370547 1102 28-29 0.6050837832435079 1102 30-31 0.5493693294987594 1102 32-33 0.7041403923768215 1102 34-35 0.5674544675125688 1102 36-37 0.6848162741096004 1102 38-39 0.9559249123926747 1102 40-41 0.8829082271197279 1102 42-43 0.8943077913388748 1102 44-45 1.016180635349194 1102 46-47 1.130343618939186 1102 48-49 1.129804580745386 1102 50-51 1.1749621568337147 1102 52-53 1.2616945894800011 1102 54-55 1.081841371516738 1102 56-57 1.0388895502276512 1102 58-59 0.8991951904773607 1102 60-61 1.0641595840391993 1102 62-63 1.111961322076919 1102 64-65 1.0044719312571253 1102 66-67 1.3326034571214969 1102 68-69 0.9504412584737345 1102 70-71 0.9253326847590309 1102 72-73 1.0491099436351305 1102 74-75 0.806454129429337 1102 76-77 1.0142006236078913 1102 78-79 1.1890030579475521 1102 80-81 1.3453826086836962 1102 82-83 1.4757517955977804 1102 84-85 1.2909333951524324 1102 86-87 1.0914762901270372 1102 88-89 1.5762965191163332 1102 90-91 1.5184993927839372 1102 92-93 1.3609015723255347 1102 94-95 1.443070773117384 1102 96-97 1.5273080023443306 1102 98-99 1.5870644057414047 1102 100-101 1.5652684373492214 1103 1 0.04038364703439257 1103 2 -0.13337470061965462 1103 3 0.027426257952683386 1103 4 0.18368497126105865 1103 5 0.2156167719560429 1103 6 0.2166673854738761 1103 7 0.3208504139650685 1103 8 0.3422233156892247 1103 9 0.18316572802197584 1103 10-11 0.2610142475853223 1103 12-13 0.36872327014354767 1103 14-15 0.37628621892466185 1103 16-17 0.45435701331877 1103 18-19 0.418898947958283 1103 20-21 0.3309347688205051 1103 22-23 0.4915173919271183 1103 24-25 0.5369202835831572 1103 26-27 0.47261350989798956 1103 28-29 0.41140525967745845 1103 30-31 0.4691955728858872 1103 32-33 0.6017593530950407 1103 34-35 0.6570367133463009 1103 36-37 0.5863069375147703 1103 38-39 0.6320572245235709 1103 40-41 0.7143264180413382 1103 42-43 0.7418622920623505 1103 44-45 0.7281378480220937 1103 46-47 0.8541939639710208 1103 48-49 0.6843958764537419 1103 50-51 0.5647644705347759 1103 52-53 0.583214074329149 1103 54-55 0.6197161194883236 1103 56-57 0.507747460704806 1103 58-59 0.610999306069818 1103 60-61 0.6511043958485878 1103 62-63 0.7969454624441603 1103 64-65 0.40840100248205147 1103 66-67 0.5374381037011702 1103 68-69 0.723265583615678 1103 70-71 0.47299860341377453 1103 72-73 0.7857982137287571 1103 74-75 0.6238085775853968 1103 76-77 0.6084648231171492 1103 78-79 0.842139208773407 1103 80-81 0.966587056484574 1103 82-83 1.0342494551720876 1103 84-85 0.9025251222271251 1103 86-87 0.7511889403795742 1103 88-89 1.2225279303750192 1103 90-91 1.6781113051575396 1103 92-93 1.5636444841144232 1103 94-95 1.6843927406867678 1103 96-97 1.8815431085091952 1103 98-99 1.8076374934302706 1103 100-101 1.270244361914738 1104 1 0.19077898285192418 1104 2 0.14060904376313843 1104 3 0.2048184824685606 1104 4 0.1947798632930713 1104 5 0.16977456422538495 1104 6 0.14145713817838157 1104 7 0.2370649639190816 1104 8 0.2750169890963292 1104 9 0.35519392414553863 1104 10-11 0.32696651097278107 1104 12-13 0.38185151203575174 1104 14-15 0.33153568595255933 1104 16-17 0.4058923211160419 1104 18-19 0.379592494021324 1104 20-21 0.46041725539169676 1104 22-23 0.46458098593668495 1104 24-25 0.3553011309967218 1104 26-27 0.4220469165306042 1104 28-29 0.4779947515947569 1104 30-31 0.32445117712974536 1104 32-33 0.3457643858662536 1104 34-35 0.40492343990779034 1104 36-37 0.29251516982122183 1104 38-39 0.25438362835136274 1104 40-41 0.39838775055671505 1104 42-43 0.41724562663667797 1104 44-45 0.5749109430772776 1104 46-47 0.6027940307052972 1104 48-49 0.5287389897731813 1104 50-51 0.5308894527139785 1104 52-53 0.5484794015494856 1104 54-55 0.4416552815080266 1104 56-57 0.3881973529413898 1104 58-59 0.25715684980098175 1104 60-61 0.32040400863219176 1104 62-63 0.4787851990241805 1104 64-65 0.36833419450270455 1104 66-67 0.6442756611248015 1104 68-69 0.6346799539512986 1104 70-71 0.5679430495382896 1104 72-73 0.7520578207588784 1104 74-75 0.7369644814219605 1104 76-77 0.49261413510093277 1104 78-79 0.5919576971173974 1104 80-81 0.8025521711768135 1104 82-83 0.9840254277302236 1104 84-85 1.181769972747162 1104 86-87 0.9189846811262932 1104 88-89 1.15887960072698 1104 90-91 1.218124555444895 1104 92-93 1.440182250610004 1104 94-95 1.3900958915563066 1104 96-97 1.255016904884247 1104 98-99 1.028018056727582 1104 100-101 0.8103728597148674 1105 1 0.2076695640935462 1105 2 0.04522778027824259 1105 3 0.13109884060400745 1105 4 0.26750236349900547 1105 5 0.23815141860998779 1105 6 0.00981578388922344 1105 7 0.20737208223618353 1105 8 0.26744094587792233 1105 9 0.3047385562193199 1105 10-11 0.3493345397997629 1105 12-13 0.3887922223080267 1105 14-15 0.4844499868163439 1105 16-17 0.4681979096943394 1105 18-19 0.4977546345100521 1105 20-21 0.40887902315437685 1105 22-23 0.4427861493437675 1105 24-25 0.4231114933086033 1105 26-27 0.5749267969576906 1105 28-29 0.395602144999458 1105 30-31 0.7144842535929001 1105 32-33 0.6491231898848255 1105 34-35 0.5915654990276522 1105 36-37 0.3528317957986431 1105 38-39 0.468338895183976 1105 40-41 0.18977310350479826 1105 42-43 0.288008053760926 1105 44-45 0.5565265542955586 1105 46-47 0.536930459390554 1105 48-49 0.6985179908632091 1105 50-51 0.6793941934722554 1105 52-53 0.6688108834924904 1105 54-55 0.757838522775458 1105 56-57 0.7932039762479377 1105 58-59 0.7759749098099924 1105 60-61 1.0012358454866792 1105 62-63 0.7294429643732308 1105 64-65 0.7588161160879601 1105 66-67 0.6819843662240324 1105 68-69 0.5203705376102405 1105 70-71 0.34726620712201495 1105 72-73 0.528869140405579 1105 74-75 0.40538564883442163 1105 76-77 0.6946850235961897 1105 78-79 0.8088172211405649 1105 80-81 0.8785905286808706 1105 82-83 1.0894898428448911 1105 84-85 1.0864159697226299 1105 86-87 0.9201348098022315 1105 88-89 1.1045380735342434 1105 90-91 1.3472124913839991 1105 92-93 1.467694229775919 1105 94-95 1.625873321130058 1105 96-97 1.6015883994813542 1105 98-99 1.486728903645055 1105 100-101 1.0909598516537855 1106 1 0.3363779338251298 1106 2 0.2595611073130435 1106 3 0.2138545743272715 1106 4 0.2834445778593704 1106 5 0.26686836403514747 1106 6 0.33774609111707576 1106 7 0.3888395723632314 1106 8 0.40711631953833205 1106 9 0.5324005459837196 1106 10-11 0.5085952714081685 1106 12-13 0.5354431232645851 1106 14-15 0.7395300678057737 1106 16-17 0.5781494933633127 1106 18-19 0.6325153363215605 1106 20-21 0.42740928009087753 1106 22-23 0.5951311654849647 1106 24-25 0.543357906415757 1106 26-27 0.6093773929683906 1106 28-29 0.6370553762190525 1106 30-31 0.7720334802542936 1106 32-33 0.7830345346553216 1106 34-35 0.6647887679366917 1106 36-37 0.7013027060285779 1106 38-39 0.8629053211982693 1106 40-41 0.8713182109262547 1106 42-43 0.7416687121125634 1106 44-45 0.8465127605104712 1106 46-47 0.7644191183991751 1106 48-49 0.8717309398698063 1106 50-51 0.8996007078016603 1106 52-53 0.7303102345014452 1106 54-55 0.852630896512899 1106 56-57 0.813063247662825 1106 58-59 0.7507872256687769 1106 60-61 0.8210595786459827 1106 62-63 0.9643001790751704 1106 64-65 0.7427755719529365 1106 66-67 0.7994383736165709 1106 68-69 1.0255774371190967 1106 70-71 0.9906722981900664 1106 72-73 1.0167106964724475 1106 74-75 1.063696731392188 1106 76-77 0.8391111861477363 1106 78-79 1.1105720406076784 1106 80-81 1.1406657593161533 1106 82-83 1.3589403879828374 1106 84-85 1.0834959460595286 1106 86-87 1.0601557548436666 1106 88-89 1.2944685377408973 1106 90-91 1.5709423802924292 1106 92-93 1.6187021644727402 1106 94-95 1.5787136925884084 1106 96-97 1.7279840209357076 1106 98-99 1.6935078440672413 1106 100-101 1.370363433968091 1107 1 0.14233059377356838 1107 2 0.06747298919727385 1107 3 0.03592003796292431 1107 4 0.11646231084898062 1107 5 0.2015453423962157 1107 6 0.20814371630260808 1107 7 0.20993948643244664 1107 8 0.1184498091094639 1107 9 0.21445663535173765 1107 10-11 0.22258245990907 1107 12-13 0.2762040013209841 1107 14-15 0.1974708536818781 1107 16-17 0.21770030970579057 1107 18-19 0.3113224607649556 1107 20-21 0.0843829540226082 1107 22-23 0.13930974528341977 1107 24-25 0.2413150972602196 1107 26-27 0.19485118407845903 1107 28-29 0.22817169525662706 1107 30-31 0.37606675890440044 1107 32-33 0.42480002172664655 1107 34-35 0.3496946346658589 1107 36-37 0.36213310990327585 1107 38-39 0.2982830260650786 1107 40-41 0.30482028107702774 1107 42-43 0.38424429214537525 1107 44-45 0.4427497732438468 1107 46-47 0.386298659060067 1107 48-49 0.44394795416556576 1107 50-51 0.519785849587457 1107 52-53 0.5625217804744125 1107 54-55 0.37559238389670213 1107 56-57 0.3600705127202488 1107 58-59 0.4318543298012649 1107 60-61 0.3193237767555068 1107 62-63 0.23711870887752795 1107 64-65 0.2910366453065798 1107 66-67 0.1197453808816249 1107 68-69 0.192418308925987 1107 70-71 0.12241825553714847 1107 72-73 0.1530430142601169 1107 74-75 0.3027796155418656 1107 76-77 0.4981089206997247 1107 78-79 0.5615602446542383 1107 80-81 0.8192525884701247 1107 82-83 0.8518925010990017 1107 84-85 0.6926694481296813 1107 86-87 0.8206635276739149 1107 88-89 0.8610563043216324 1107 90-91 1.1019962042124334 1107 92-93 0.9136533401621385 1107 94-95 1.0143101303667024 1107 96-97 1.2632000196858044 1107 98-99 1.051140060300348 1107 100-101 0.9507126254375073 1108 1 0.03397594756726008 1108 2 -0.06697429696356849 1108 3 0.028297607348164888 1108 4 0.07242328461046554 1108 5 0.044957296549853254 1108 6 0.03002303807963358 1108 7 0.08037616351874277 1108 8 0.1646504342870685 1108 9 0.12917672628665855 1108 10-11 0.04719882819551202 1108 12-13 0.04582510580142696 1108 14-15 -0.024152240000034908 1108 16-17 -0.020151826317707844 1108 18-19 0.007053255498313149 1108 20-21 -0.023127702413880513 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-1.8116639477086167 2316 58-59 -1.812513165384022 2316 60-61 -1.0526333884441286 2316 62-63 -0.9656098446401238 2316 64-65 -1.2609452074213259 2316 66-67 -1.4832229389446567 2316 68-69 -1.3097985277097877 2316 70-71 -1.061017317769302 2316 72-73 -0.9790602080439648 2316 74-75 -1.1865788964852264 2316 76-77 -1.5781368946996075 2316 78-79 -1.4885179540609776 2316 80-81 -1.5321611925993217 2316 82-83 -1.2524751233403606 2316 84-85 -1.1130792560982208 2316 86-87 -0.9911421517501289 2316 88-89 -1.3482096617879478 2316 90-91 -1.6968054812607036 2316 92-93 -1.6343980254071617 2316 94-95 -1.5886070048459828 2316 96-97 -1.1560995524832087 2316 98-99 -0.9901592016588872 2316 100-101 -0.9051096243589196 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 24.0 4 50.0 5 120.0 6 282.0 7 725.0 8 1637.0 9 2820.0 10 4610.0 11 6881.0 12 9602.0 13 12799.0 14 16672.0 15 21505.0 16 26629.0 17 32405.0 18 30773.0 19 26042.0 20 26433.0 21 27598.0 22 30414.0 23 33363.0 24 38745.0 25 44947.0 26 53426.0 27 63979.0 28 75840.0 29 88741.0 30 104219.0 31 123034.0 32 144902.0 33 172112.0 34 206688.0 35 245052.0 36 266410.0 37 212612.0 38 65133.0 39 1780.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.1987535200349 22.028838145099357 16.386190646172043 21.3862176886937 2 17.585350333397628 23.131251768837103 36.91294887903047 22.3704490187348 3 21.057164256994447 25.407958813397542 30.417030573589415 23.117846356018593 4 15.905665955237655 19.356937874942318 35.170445532418086 29.56695063740194 5 14.232246870739868 37.74170506652846 33.80484232584865 14.22120573688302 6 31.54558011360948 37.55210105430136 16.600999096441875 14.301319735647283 7 27.63192938829901 33.35944108383517 20.83204709850208 18.176582429363737 8 26.402539871384086 35.45189070801844 19.339299958089416 18.806269462508055 9 26.07590772461593 16.67266033050775 20.066471083951853 37.18496086092446 10-11 25.00878207541792 27.127205686683943 27.051293404238873 20.812718833659265 12-13 26.060821554939512 24.34716636934799 27.690091863472706 21.901920212239794 14-15 23.04200972505757 25.626247741109776 26.03163122293275 25.300111310899904 16-17 22.213532160738346 28.773867625652883 26.654769469267826 22.357830744340944 18-19 22.333180111851682 27.193793628690273 29.193311431674484 21.27971482778356 20-21 23.041392102836717 26.24897813133457 29.41073216720881 21.298897598619902 22-23 22.768351652313417 26.160111941811888 29.355230485667033 21.71630592020766 24-25 22.661535989811355 26.40857201894328 29.327104534799382 21.602787456445995 26-27 22.53184528591074 26.507495255119434 29.26462970102577 21.696029757944054 28-29 22.544712669889215 26.59925027467996 29.269955194943908 21.586081860486917 30-31 22.735754195735332 26.406980355075245 29.14366751234146 21.713597936847968 32-33 22.617260828154002 26.334280533691935 29.217139016280115 21.831319621873952 34-35 22.79097362552975 26.47764902900633 29.070120110105435 21.661257235358484 36-37 22.934727573567272 26.429123655297342 29.005806154609516 21.630342616525873 38-39 22.745562396840864 26.5212458525099 28.988727840150517 21.744463910498713 40-41 22.825101861562015 26.379541971109667 28.93974743830734 21.855608729020982 42-43 22.788344085662917 26.478716651573368 28.752873227814717 21.980066034948994 44-45 22.90907878240539 26.586707153408877 28.581954557868013 21.922259506317715 46-47 23.155775012978772 26.818244909437013 28.139826444970005 21.886153632614214 48-49 23.176683614194822 26.923609039362667 27.866283371924183 22.03342397451832 50-51 23.078245566993353 27.13884473125936 27.67548104276349 22.107428658983803 52-53 23.241767651048452 27.23872122001451 27.40823527821216 22.11127585072488 54-55 23.136984239435122 27.21577174130537 27.38749664256524 22.259747376694268 56-57 23.076370160182407 27.36451507581225 27.41512342375614 22.143991340249205 58-59 23.258530465239108 27.176194733815596 27.473965609548134 22.09130919139716 60-61 23.16026138913168 27.148178417167628 27.689257795380378 22.002302398320317 62-63 22.999783687320022 27.102266325974195 27.78378646333275 22.114163523373033 64-65 22.932312275786483 27.087595281942615 27.695389806203814 22.284702636067085 66-67 23.008333900403287 27.193287634254748 27.49324135233341 22.305137113008556 68-69 22.963889301986022 27.0738302215853 27.59408474950541 22.36819572692327 70-71 23.019114847715734 27.067455367443472 27.483069011017534 22.430360773823256 72-73 23.10972530762036 27.223520223775516 27.263019829342777 22.403734639261348 74-75 23.079238739199543 27.390386583524222 27.007894987073023 22.522479690203212 76-77 23.083651682583003 27.263937478115132 27.185658939008086 22.46675190029378 78-79 23.05974110954084 27.337485939663132 27.12797790724504 22.47479504355099 80-81 23.034480707311815 27.277256469484367 27.13464310492887 22.55361971827495 82-83 23.088180784748033 27.366045626562506 27.053944526626385 22.491829062063076 84-85 23.057610564911258 27.36587458674483 26.995341978793746 22.58117286955017 86-87 23.02497627294629 27.347558749807344 26.98734917660589 22.640115800640473 88-89 23.008328314172648 27.307794311527754 26.88426953354816 22.799607840751438 90-91 23.09040796608214 27.328968620212272 26.906257210429164 22.67436620327642 92-93 22.962570228421786 27.35317882892769 26.893987361024674 22.79026358162585 94-95 22.97337886419942 27.323093770525787 26.81273046621068 22.89079689906411 96-97 23.00506424664402 27.295331767303782 26.766847492381096 22.9327564936711 98-99 22.866122542054292 27.359837438603464 26.57735974730698 23.196680272035266 100-101 23.060429131012402 27.346173674941284 26.40261631837101 23.190780875675305 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 809.0 1 563.5 2 361.5 3 433.5 4 429.0 5 435.5 6 715.5 7 1521.0 8 2552.5 9 3060.0 10 2697.5 11 2226.0 12 2226.5 13 2370.5 14 2618.0 15 2922.5 16 3091.0 17 3244.0 18 3614.0 19 3967.5 20 4337.5 21 4997.5 22 5978.0 23 7355.0 24 8975.5 25 11184.0 26 13938.5 27 17121.5 28 20404.0 29 23467.5 30 26663.0 31 30357.0 32 34275.5 33 37812.0 34 41469.0 35 44703.5 36 48867.5 37 53669.0 38 56364.5 39 57437.0 40 59661.0 41 63395.5 42 66157.0 43 69375.0 44 73500.0 45 76454.0 46 77973.0 47 79732.5 48 81760.5 49 81675.5 50 80733.0 51 80291.0 52 80487.5 53 80498.0 54 78571.0 55 73686.5 56 67460.0 57 61881.0 58 56677.5 59 50503.0 60 42729.0 61 35066.5 62 28112.0 63 22083.0 64 16656.0 65 11929.5 66 8831.5 67 6866.5 68 5115.5 69 3747.0 70 2794.5 71 1958.0 72 1329.5 73 933.5 74 619.0 75 364.5 76 221.0 77 133.5 78 76.0 79 44.5 80 28.5 81 18.0 82 12.0 83 8.5 84 7.5 85 6.0 86 5.5 87 3.0 88 2.0 89 1.0 90 1.0 91 1.5 92 0.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012708369948760933 2 0.001712475383166367 3 0.0016223450998418213 4 9.013028332454563E-5 5 0.0015772799581795485 6 2.2532570831136409E-4 7 4.506514166227282E-5 8 0.0 9 0.0 10-11 6.759771249340923E-5 12-13 4.7318398745386457E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0016223450998418213 22-23 0.0 24-25 0.0031320273455279603 26-27 0.0017800730956597762 28-29 0.0028841690663854603 30-31 0.012122523107151386 32-33 6.534445541029559E-4 34-35 0.0020729965164645496 36-37 0.003222157628852506 38-39 0.002140594228957959 40-41 0.003019364491372279 42-43 0.0015096822456861394 44-45 5.407816999472738E-4 46-47 9.013028332454563E-5 48-49 1.8026056664909127E-4 50-51 1.1266285415568204E-4 52-53 9.013028332454563E-4 54-55 0.002433517649762732 56-57 3.8305370412931893E-4 58-59 9.91433116570002E-4 60-61 8.7877026241432E-4 62-63 0.0 64-65 1.5772799581795487E-4 66-67 4.506514166227282E-5 68-69 0.0 70-71 9.013028332454563E-5 72-73 2.253257083113641E-5 74-75 3.1545599163590975E-4 76-77 4.95716558285001E-4 78-79 9.013028332454563E-5 80-81 0.003312287912177052 82-83 7.886399790897743E-4 84-85 0.0018927359498154583 86-87 0.0010815633998945476 88-89 6.08379412440683E-4 90-91 9.013028332454563E-5 92-93 4.506514166227282E-5 94-95 1.1266285415568204E-4 96-97 0.0010139656874011383 98-99 0.001487149674855003 100-101 6.309119832718195E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2219010.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.638288552762596 #Duplication Level Percentage of deduplicated Percentage of total 1 76.30889918961192 45.509321490137474 2 13.50762260072211 16.111429886473655 3 4.4599809351866595 7.979568898574459 4 2.033485992305682 4.850944975085081 5 1.034295954389625 3.0841820288421715 6 0.6449956132327658 2.3079860698345045 7 0.4221841179281888 1.7624836773176413 8 0.28959865836389803 1.3816934681599253 9 0.21804653799668275 1.1703530115879373 >10 1.0127417526905123 10.570809226748782 >50 0.04935772438249942 1.983108184641736 >100 0.01758019872803552 1.748290924663534 >500 7.567027884462286E-4 0.2898149652780095 >1k 2.2701083653372498E-4 0.21178785924151414 >5k 2.2701083653372498E-4 1.0382253334136533 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9505 0.4283441714999031 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8191 0.3691285753556766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5310 0.23929590222666863 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.506514166227282E-5 2 0.0 0.0 0.0 0.0 4.506514166227282E-5 3 0.0 0.0 0.0 0.0 4.506514166227282E-5 4 0.0 0.0 0.0 4.506514166227282E-5 4.506514166227282E-5 5 0.0 0.0 0.0 4.506514166227282E-5 4.506514166227282E-5 6 0.0 0.0 0.0 4.506514166227282E-5 4.506514166227282E-5 7 0.0 0.0 0.0 4.506514166227282E-5 4.506514166227282E-5 8 0.0 0.0 0.0 4.506514166227282E-5 4.506514166227282E-5 9 0.0 0.0 0.0 4.506514166227282E-5 4.506514166227282E-5 10-11 0.0 0.0 0.0 4.506514166227282E-5 4.506514166227282E-5 12-13 0.0 0.0 0.0 4.506514166227282E-5 9.013028332454563E-5 14-15 0.0 0.0 0.0 4.506514166227282E-5 1.1266285415568204E-4 16-17 0.0 0.0 0.0 4.506514166227282E-5 1.3519542498681845E-4 18-19 0.0 0.0 0.0 4.506514166227282E-5 1.3519542498681845E-4 20-21 0.0 0.0 0.0 6.759771249340923E-5 1.8026056664909127E-4 22-23 0.0 0.0 0.0 2.2532570831136409E-4 1.8026056664909127E-4 24-25 0.0 0.0 0.0 3.154559916359097E-4 1.8026056664909127E-4 26-27 0.0 0.0 0.0 4.281188457915918E-4 2.0279313748022766E-4 28-29 0.0 0.0 0.0 9.013028332454563E-4 2.703908499736369E-4 30-31 0.0 0.0 0.0 0.0022307245122825045 2.703908499736369E-4 32-33 0.0 0.0 0.0 0.0045741118787206905 2.703908499736369E-4 34-35 0.0 0.0 0.0 0.007638541511755243 2.703908499736369E-4 36-37 0.0 0.0 0.0 0.012392913957125024 2.929234208047733E-4 38-39 0.0 0.0 0.0 0.019175217777297082 3.3798856246704614E-4 40-41 0.0 0.0 0.0 0.02721934556401278 3.6052113329818254E-4 42-43 0.0 0.0 0.0 0.03679568816724575 3.6052113329818254E-4 44-45 0.0 0.0 0.0 0.05101374036169283 3.6052113329818254E-4 46-47 0.0 0.0 0.0 0.06928765530574446 3.6052113329818254E-4 48-49 0.0 0.0 0.0 0.08740384225397813 4.055862749604553E-4 50-51 0.0 0.0 0.0 0.10779581885615658 4.055862749604553E-4 52-53 0.0 0.0 0.0 0.1287285771582823 5.182491291161374E-4 54-55 0.0 0.0 0.0 0.1524328416726378 5.858468416095466E-4 56-57 0.0 0.0 0.0 0.18483467852781194 5.858468416095466E-4 58-59 0.0 0.0 0.0 0.21937710961194407 5.858468416095466E-4 60-61 0.0 0.0 0.0 0.25655585148331916 5.858468416095466E-4 62-63 0.0 0.0 0.0 0.2983087052334149 5.858468416095466E-4 64-65 0.0 0.0 0.0 0.34350904232067453 5.858468416095466E-4 66-67 0.0 0.0 0.0 0.3945903803948608 5.858468416095466E-4 68-69 0.0 0.0 0.0 0.4525666851433747 5.858468416095466E-4 70-71 0.0 0.0 0.0 0.5236569461156101 6.985096957652287E-4 72-73 0.0 0.0 0.0 0.5972933875917639 7.210422665963651E-4 74-75 0.0 0.0 0.0 0.6799653899712034 7.210422665963651E-4 76-77 0.0 0.0 0.0 0.7769681073992456 7.210422665963651E-4 78-79 4.506514166227282E-5 0.0 0.0 0.8822402783223149 7.210422665963651E-4 80-81 4.506514166227282E-5 0.0 0.0 0.9980126272526937 7.210422665963651E-4 82-83 4.506514166227282E-5 0.0 0.0 1.121423517694828 8.111725499209107E-4 84-85 4.506514166227282E-5 0.0 0.0 1.250918202261369 8.111725499209107E-4 86-87 4.506514166227282E-5 0.0 0.0 1.3880739609104962 8.33705120752047E-4 88-89 6.759771249340923E-5 0.0 0.0 1.5435486996453374 8.562376915831834E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6175 0.0 46.390457 1 GTATCAA 9685 0.0 35.512993 1 TATCAAC 12365 0.0 27.620045 2 ATCAACG 12420 0.0 27.45887 3 TCAACGC 12525 0.0 27.190752 4 CAACGCA 12740 0.0 26.6946 5 AACGCAG 13140 0.0 25.918127 6 ACCGCGT 100 0.0016544348 23.749245 8 ACGCAGA 15125 0.0 22.35962 7 CGCAGAG 15360 0.0 21.801064 8 GAGTACT 9330 0.0 21.661716 12-13 GTACTTT 9475 0.0 21.080278 14-15 GCAGAGT 16000 0.0 20.246233 9 AGAGTAC 13560 0.0 19.738495 10-11 AGTACTT 9500 0.0 19.399164 12-13 TATTCCG 250 2.1750384E-7 18.999397 5 CAGAGTA 15635 0.0 18.54674 10-11 TTACGCG 80 0.001191908 17.812136 80-81 GTACATG 7610 0.0 17.354353 1 ACTTTTT 11725 0.0 17.298347 16-17 >>END_MODULE