FastQCFastQC Report
Thu 26 May 2016
SRR936774_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936774_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066727
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99130.47964728771627796No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68420.3310548514632073No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44500.21531629479849054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40700.051.960671
GTATCAA78100.042.7164541
TCAACGC95950.034.521244
ATCAACG96000.034.5032583
TATCAAC97300.034.0919382
CAACGCA97450.033.992345
AACGCAG101050.032.9200366
ACGCAGA117050.028.2969047
CGCAGAG116500.028.268028
GCAGAGT127000.025.3696259
GTACATG73450.023.4221021
GAGTACT74450.023.1693712-13
TACATGG75450.022.4233172
GTACTTT77250.022.08244714-15
ACATGGG72750.021.6218873
AGAGTAC110900.021.5104610-11
GTGGTAT21750.020.7574231
CAGAGTA122200.020.74630510-11
AGTACTT76900.020.26841512-13
TGGTATC22000.019.8734972
Produced by FastQC (version 0.11.5)