FastQCFastQC Report
Thu 26 May 2016
SRR936774_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936774_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2066727
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88310.42729397738549885No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73920.3576669777866162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48080.23263836975081859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53950.047.8274651
GTATCAA88200.035.612451
ATCAACG112250.027.6329753
TATCAAC113700.027.450342
TCAACGC112650.027.448534
CAACGCA114300.027.0100825
AACGCAG118800.025.9850866
ACGCAGA133900.022.7709777
CGCAGAG135100.022.4281048
GAGTACT87000.021.6181712-13
GTACTTT88950.020.85234514-15
GCAGAGT144700.020.3493429
AGAGTAC122250.020.27910810-11
AGTACTT88950.020.02296312-13
GTGGTAT28500.019.1744081
CAGAGTA139850.018.89861510-11
GTACATG66850.018.6238231
ACTTTTT104000.017.81279416-17
TACTTTT100000.017.71693614-15
GGCGTAA1350.00938102917.5996971
Produced by FastQC (version 0.11.5)