##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936774_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2066727 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.389970712145338 31.0 31.0 34.0 30.0 34.0 2 31.503775293011607 31.0 31.0 34.0 30.0 34.0 3 31.614146425725313 31.0 31.0 34.0 30.0 34.0 4 35.45091296528279 37.0 35.0 37.0 33.0 37.0 5 35.289934277725116 37.0 35.0 37.0 33.0 37.0 6 35.34926431986421 37.0 35.0 37.0 33.0 37.0 7 35.294730750602284 37.0 35.0 37.0 32.0 37.0 8 35.25905501790996 37.0 35.0 37.0 32.0 37.0 9 36.80947991679598 39.0 37.0 39.0 33.0 39.0 10-11 36.643941604285416 38.0 35.0 39.0 32.0 39.0 12-13 36.52852457049238 38.0 35.0 39.0 32.0 39.0 14-15 37.48605403616443 39.0 36.0 40.0 32.0 41.0 16-17 37.329105634174226 39.0 36.0 40.0 32.0 41.0 18-19 37.33466926207477 39.0 36.0 40.0 32.0 41.0 20-21 37.450138794335196 39.0 36.0 40.0 32.0 41.0 22-23 37.34493936547982 39.0 36.0 40.0 32.0 41.0 24-25 37.204959581018684 39.0 36.0 40.0 31.0 41.0 26-27 36.91821222638501 39.0 36.0 40.0 31.0 41.0 28-29 36.68002909915049 38.5 35.5 40.0 30.0 41.0 30-31 36.60040368176348 38.0 35.0 40.0 30.0 41.0 32-33 36.45943561970207 38.0 35.0 40.0 30.0 41.0 34-35 36.28071172438353 38.0 35.0 40.0 30.0 41.0 36-37 36.038718950301615 38.0 35.0 40.0 30.0 41.0 38-39 35.81309964983281 38.0 35.0 40.0 29.0 41.0 40-41 35.502834191453445 38.0 34.0 40.0 27.5 41.0 42-43 35.17472360887529 38.0 34.0 40.0 27.0 41.0 44-45 34.839425332905606 38.0 33.0 40.0 26.0 41.0 46-47 34.42596724192407 38.0 33.0 40.0 24.5 41.0 48-49 33.96095976875514 37.5 33.0 40.0 23.5 41.0 50-51 33.624459592389314 37.0 32.5 40.0 23.0 41.0 52-53 33.285057726540565 37.0 32.0 39.5 22.0 40.0 54-55 32.89538579599531 36.5 31.0 39.0 20.0 40.0 56-57 32.51234415575932 36.0 31.0 39.0 19.0 40.0 58-59 32.00180720530578 35.5 30.5 39.0 17.5 40.0 60-61 31.512753256719442 35.0 29.5 38.0 13.5 40.0 62-63 31.046578720847023 35.0 29.0 38.0 12.0 40.0 64-65 30.491261303500657 34.0 28.0 38.0 8.0 40.0 66-67 29.894728234546704 34.0 28.0 37.0 7.5 39.0 68-69 29.3125635364516 33.5 26.5 36.5 6.5 39.0 70-71 28.768024272194637 33.0 26.0 36.0 2.0 39.0 72-73 28.196047421841396 32.5 26.0 35.5 2.0 38.5 74-75 27.556195617515037 32.0 25.5 35.0 2.0 37.5 76-77 26.494700316006902 30.5 24.0 34.0 2.0 36.0 78-79 27.162243731271715 32.0 25.5 35.0 2.0 36.0 80-81 26.800372763311266 32.0 25.0 35.0 2.0 36.0 82-83 26.401820608140312 32.0 24.0 34.5 2.0 36.0 84-85 25.842714833647598 31.0 22.5 34.0 2.0 35.5 86-87 25.218388059961477 31.0 20.0 34.0 2.0 35.0 88-89 24.557635575477555 31.0 18.5 34.0 2.0 35.0 90-91 23.76007498813341 30.0 13.0 34.0 2.0 35.0 92-93 22.942830378661526 29.5 4.5 34.0 2.0 35.0 94-95 21.973804232489343 29.0 2.0 34.0 2.0 35.0 96-97 20.792526298829017 29.0 2.0 33.5 2.0 34.5 98-99 19.401512633260225 26.5 2.0 33.0 2.0 34.0 100-101 16.22074637821057 13.0 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3878325167154415 1101 2 0.3838526051470055 1101 3 0.3715228968038815 1101 4 0.3398991856861997 1101 5 0.3759000298505981 1101 6 0.33148696545186596 1101 7 0.22077836962986197 1101 8 0.3328158399869565 1101 9 0.4099132575036464 1101 10-11 0.37339699831854745 1101 12-13 0.3360840912736762 1101 14-15 0.48371589140975146 1101 16-17 0.4632160024965586 1101 18-19 0.4707257544115109 1101 20-21 0.5032510694054722 1101 22-23 0.5651081180330877 1101 24-25 0.49160689632746823 1101 26-27 0.5654370605683496 1101 28-29 0.568594200382087 1101 30-31 0.4573954545335752 1101 32-33 0.6330962316092297 1101 34-35 0.5627175294592135 1101 36-37 0.7177123839823523 1101 38-39 0.5158043553317242 1101 40-41 0.7535224608264954 1101 42-43 0.8855246096840048 1101 44-45 0.7436999512029843 1101 46-47 0.9852169745555273 1101 48-49 0.9125836915627445 1101 50-51 0.7780993062433339 1101 52-53 0.9141693664571946 1101 54-55 0.8495479438814115 1101 56-57 0.8385240531649387 1101 58-59 0.7462378656859414 1101 60-61 0.77882792973843 1101 62-63 0.7771571353950932 1101 64-65 0.8936943598223266 1101 66-67 0.8224425878422359 1101 68-69 0.7809058062084979 1101 70-71 0.7787577772529275 1101 72-73 0.8444447362112371 1101 74-75 0.6991437465684776 1101 76-77 0.6461440114903247 1101 78-79 1.1280512471173196 1101 80-81 1.6355478219589195 1101 82-83 1.3907986885274397 1101 84-85 0.9853939008021584 1101 86-87 0.8775986606703263 1101 88-89 0.7873879969018311 1101 90-91 0.5871853298968439 1101 92-93 1.0356566780845462 1101 94-95 1.174426433051515 1101 96-97 1.0695652239594295 1101 98-99 1.3551115008105619 1101 100-101 1.292083452319444 1102 1 0.32221321902968825 1102 2 0.3147755051181491 1102 3 0.3093256627929435 1102 4 0.29218530555736777 1102 5 0.3176569175021271 1102 6 0.3217642090329562 1102 7 0.2708382462636578 1102 8 0.17025867966432173 1102 9 0.35811344782641896 1102 10-11 0.37980979096911227 1102 12-13 0.2969662801901123 1102 14-15 0.3395978154463606 1102 16-17 0.5050723608595575 1102 18-19 0.442395217925835 1102 20-21 0.35886638987157937 1102 22-23 0.3493840003271558 1102 24-25 0.3574718025525385 1102 26-27 0.3993327512319027 1102 28-29 0.2955981772569771 1102 30-31 0.5713223998691532 1102 32-33 0.46612494354970124 1102 34-35 0.4100934206132436 1102 36-37 0.4422342471414069 1102 38-39 0.49290328099978353 1102 40-41 0.6244857006758266 1102 42-43 0.43635671734956105 1102 44-45 0.5073358332396367 1102 46-47 0.5365899886893075 1102 48-49 0.4584622014648332 1102 50-51 0.5445972695414412 1102 52-53 0.4172704141517656 1102 54-55 0.47115468086170154 1102 56-57 0.5631073960266733 1102 58-59 0.5247797601961892 1102 60-61 0.5021453078440068 1102 62-63 0.5252455475743218 1102 64-65 0.5040534139266484 1102 66-67 0.6316791719656045 1102 68-69 0.49774529212786334 1102 70-71 0.516250995811891 1102 72-73 0.5374970356727786 1102 74-75 0.6754636612001761 1102 76-77 0.7003919199450905 1102 78-79 0.8358114450199103 1102 80-81 0.7864798599972715 1102 82-83 0.8192224098559215 1102 84-85 0.754534112331406 1102 86-87 0.8489896268772874 1102 88-89 0.7203965526930531 1102 90-91 0.7783802528455368 1102 92-93 0.8356877396988125 1102 94-95 1.0796399578046803 1102 96-97 1.2456494127703763 1102 98-99 1.4115706100857324 1102 100-101 1.3468391141691267 1103 1 0.2553735173463423 1103 2 0.1449417646036295 1103 3 0.24140462438351662 1103 4 0.1886575067775098 1103 5 0.2000385582437474 1103 6 0.2905965012720202 1103 7 0.2736952719851615 1103 8 0.12335960638846899 1103 9 0.24939459854627444 1103 10-11 0.2597069507274412 1103 12-13 0.2856056759099772 1103 14-15 0.2811385300164986 1103 16-17 0.3061370381142936 1103 18-19 0.3819342477488945 1103 20-21 0.39007911557853703 1103 22-23 0.36162639008585273 1103 24-25 0.23852527232063636 1103 26-27 0.31798605891275145 1103 28-29 0.3604005762208544 1103 30-31 0.28714381260138566 1103 32-33 0.217189971887791 1103 34-35 0.3649736037926843 1103 36-37 0.34503438603451286 1103 38-39 0.47719654794589417 1103 40-41 0.5430634688095068 1103 42-43 0.3741241919005489 1103 44-45 0.43240785062800313 1103 46-47 0.6554411035261865 1103 48-49 0.5101980708128977 1103 50-51 0.49059781859050133 1103 52-53 0.47631821720805334 1103 54-55 0.5314971320939605 1103 56-57 0.48238818026081276 1103 58-59 0.6362074440472156 1103 60-61 0.5437566694449103 1103 62-63 0.5330217040768623 1103 64-65 0.5685541643390053 1103 66-67 0.607178706950041 1103 68-69 0.46018939626054234 1103 70-71 0.4492576102222543 1103 72-73 0.48616836638741034 1103 74-75 0.3327502489553851 1103 76-77 0.3186575749782925 1103 78-79 0.5362458936808743 1103 80-81 0.7434318879679722 1103 82-83 0.6765443437770493 1103 84-85 0.5296359491542049 1103 86-87 0.45233276713893744 1103 88-89 0.4140074132640379 1103 90-91 0.25807574353095575 1103 92-93 0.28031209863193496 1103 94-95 0.6454756177258645 1103 96-97 0.9738487221101444 1103 98-99 1.1837527924470344 1103 100-101 1.0378470880648347 1104 1 0.19252126952036264 1104 2 0.1495438572533061 1104 3 0.1895805096279588 1104 4 0.1648165342216501 1104 5 0.026719149826867294 1104 6 0.1688269952169179 1104 7 0.16390380222104284 1104 8 0.15168614027224692 1104 9 0.12906807706706047 1104 10-11 0.19205586673567154 1104 12-13 0.2670327063102178 1104 14-15 0.22798826019340623 1104 16-17 0.3061595213836412 1104 18-19 0.16217561667823333 1104 20-21 0.32425698889264964 1104 22-23 0.2525540271169078 1104 24-25 0.13409823905780627 1104 26-27 0.4349071701897813 1104 28-29 0.29775717828678694 1104 30-31 0.45037741722885727 1104 32-33 0.3024278031313514 1104 34-35 0.2888294498959638 1104 36-37 0.325501911605798 1104 38-39 0.5051548213834849 1104 40-41 0.36731837207062057 1104 42-43 0.4385757337160925 1104 44-45 0.5732325536460792 1104 46-47 0.42038049054042403 1104 48-49 0.5692914976829897 1104 50-51 0.4924722195336244 1104 52-53 0.49170834308095834 1104 54-55 0.4282036849948838 1104 56-57 0.36889484318066224 1104 58-59 0.5171720108918265 1104 60-61 0.6023420459925575 1104 62-63 0.6246391779068148 1104 64-65 0.5818859081389895 1104 66-67 0.4983247954814054 1104 68-69 0.6558163228441423 1104 70-71 0.6329299379813271 1104 72-73 0.6005316926803559 1104 74-75 0.7270904526669248 1104 76-77 0.7901370408950861 1104 78-79 1.0627957721877301 1104 80-81 1.1453192540642974 1104 82-83 1.0648380986046924 1104 84-85 0.8178454006673945 1104 86-87 1.053926908464117 1104 88-89 0.7112786361582764 1104 90-91 0.7646440785953637 1104 92-93 0.9085203478567685 1104 94-95 0.8421223424681479 1104 96-97 1.0573289222816165 1104 98-99 1.279058417009594 1104 100-101 0.9947105205122124 1105 1 0.16299190915829342 1105 2 0.1139568354978664 1105 3 0.09589195184290489 1105 4 0.18532895971812735 1105 5 0.18430889925598137 1105 6 0.19642395283544545 1105 7 0.17834310916315843 1105 8 0.15576232243591903 1105 9 -0.05081824066621721 1105 10-11 0.13511336016122755 1105 12-13 0.18841024334557233 1105 14-15 0.2995462853453006 1105 16-17 0.3121710689133792 1105 18-19 0.29763167763714904 1105 20-21 0.28297091187889833 1105 22-23 -0.06637898566925315 1105 24-25 0.21811505799468023 1105 26-27 0.2879442833485726 1105 28-29 0.25787731649441525 1105 30-31 0.2512819702902007 1105 32-33 0.1650010829554276 1105 34-35 0.16774056246119073 1105 36-37 0.12929064209886576 1105 38-39 0.2912353774084764 1105 40-41 0.15941491955913278 1105 42-43 0.22420532706910024 1105 44-45 0.433108430067108 1105 46-47 0.39200880713192987 1105 48-49 0.6440647411312099 1105 50-51 0.6521607294014871 1105 52-53 0.6942233648652518 1105 54-55 0.4295592780140254 1105 56-57 0.3774440701165176 1105 58-59 0.5922893049818327 1105 60-61 0.699636695032563 1105 62-63 0.6241730800980214 1105 64-65 0.4688156161841306 1105 66-67 0.35590470385135475 1105 68-69 0.39652103027099983 1105 70-71 0.2443944885283038 1105 72-73 0.3978488962944482 1105 74-75 0.283818748751937 1105 76-77 0.47225588791453177 1105 78-79 0.7684536212310356 1105 80-81 0.8606250121659116 1105 82-83 0.7518486862447347 1105 84-85 0.20076146364605307 1105 86-87 0.1445045193847072 1105 88-89 0.32518812314540924 1105 90-91 0.5138654885151475 1105 92-93 0.679704000569398 1105 94-95 0.6127706621717017 1105 96-97 0.7012714798646904 1105 98-99 0.6404322855454083 1105 100-101 0.4827482880241458 1106 1 0.13412077582793813 1106 2 0.11822691093419024 1106 3 0.1754467268730373 1106 4 0.1664924757650894 1106 5 0.16280299044912994 1106 6 0.06408146522218061 1106 7 0.011054966950062806 1106 8 0.0507042665951829 1106 9 0.22726728359392467 1106 10-11 0.1936695524122669 1106 12-13 0.016551703194707557 1106 14-15 0.2857416330665856 1106 16-17 0.13090544600573395 1106 18-19 0.14394655455095773 1106 20-21 0.16147490687956179 1106 22-23 0.11073373360941474 1106 24-25 0.12727070958152353 1106 26-27 0.19627219981065025 1106 28-29 0.1908230994252733 1106 30-31 0.0253825371785652 1106 32-33 0.07956574784405746 1106 34-35 0.1568001331660085 1106 36-37 0.2794101852404083 1106 38-39 0.21018938189448022 1106 40-41 0.24010779551324646 1106 42-43 0.2795636356661646 1106 44-45 0.3163811083914396 1106 46-47 0.5995313069087445 1106 48-49 0.6694812590082861 1106 50-51 0.16552270938183966 1106 52-53 0.22730270970745892 1106 54-55 0.406424541594788 1106 56-57 0.22183614372273297 1106 58-59 0.34006345911296876 1106 60-61 0.4599795481102795 1106 62-63 0.32139534106753587 1106 64-65 0.4223565761002135 1106 66-67 0.3740706581878257 1106 68-69 0.45398361898679696 1106 70-71 0.5090299447172093 1106 72-73 0.2911871210857697 1106 74-75 0.5496285733289987 1106 76-77 0.45117779569474337 1106 78-79 0.5212347080274462 1106 80-81 0.9901334764340888 1106 82-83 0.8454610791162231 1106 84-85 0.5932776026676123 1106 86-87 0.43873722538390325 1106 88-89 0.1639279124593429 1106 90-91 0.27157434726174756 1106 92-93 0.5320272106305488 1106 94-95 0.7977318001889273 1106 96-97 0.9280850623090053 1106 98-99 0.6446941310571717 1106 100-101 0.5231133479491543 1107 1 0.14821981613503965 1107 2 0.08049869596298365 1107 3 0.04319950998243982 1107 4 0.029967331811157294 1107 5 0.11309320158156311 1107 6 -0.011463256466882399 1107 7 0.02791869055850782 1107 8 0.08341290383126676 1107 9 -0.047906612759241796 1107 10-11 -0.09243140728063537 1107 12-13 0.11705035962464194 1107 14-15 0.10860151790343764 1107 16-17 0.13089316193663336 1107 18-19 0.20799185205576265 1107 20-21 0.23828719094866102 1107 22-23 0.0733663631679562 1107 24-25 0.18018395334351567 1107 26-27 0.1929631786974042 1107 28-29 0.26352789788977304 1107 30-31 0.08396104005764471 1107 32-33 0.25398916435077723 1107 34-35 0.17639782990305264 1107 36-37 0.19084703744769627 1107 38-39 0.171492644850332 1107 40-41 0.5896125939777406 1107 42-43 0.3890910828830556 1107 44-45 0.3375715114624569 1107 46-47 0.4587032838761189 1107 48-49 0.6222659039219067 1107 50-51 0.42562904335497365 1107 52-53 0.28294109742338946 1107 54-55 0.2005197431303003 1107 56-57 0.25739813988396065 1107 58-59 0.3826561654469458 1107 60-61 0.2503119857302387 1107 62-63 0.3941826731212785 1107 64-65 0.1570836394399464 1107 66-67 0.2698971457118198 1107 68-69 0.31282277445704665 1107 70-71 0.1647090234120263 1107 72-73 0.25209191955026 1107 74-75 0.08020246968216682 1107 76-77 0.1445186786122079 1107 78-79 0.30279286541708217 1107 80-81 0.40710698891009756 1107 82-83 0.4418100234540354 1107 84-85 0.3793931496925609 1107 86-87 0.4452024845009852 1107 88-89 0.04467416965703919 1107 90-91 0.09197856991049846 1107 92-93 0.24688248894149112 1107 94-95 0.2144212435670525 1107 96-97 0.36339677056236397 1107 98-99 0.3462165878709911 1107 100-101 0.406337532210193 1108 1 0.027759302017887677 1108 2 -0.00337269046769606 1108 3 -0.01892000118485626 1108 4 0.127584188111193 1108 5 -0.1952235282962249 1108 6 -0.01662785823678803 1108 7 0.07923853885181131 1108 8 0.039478811964436034 1108 9 -0.004622668385032114 1108 10-11 -0.07025618604169637 1108 12-13 -0.05587732684817581 1108 14-15 0.012212980187463529 1108 16-17 0.050793372640391965 1108 18-19 0.04548076903848397 1108 20-21 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Count 2 1.0 3 12.0 4 29.0 5 85.0 6 220.0 7 578.0 8 1334.0 9 2437.0 10 3862.0 11 5644.0 12 8138.0 13 11211.0 14 14303.0 15 18532.0 16 23331.0 17 28745.0 18 27236.0 19 23391.0 20 23452.0 21 25486.0 22 27183.0 23 30479.0 24 34779.0 25 40983.0 26 47751.0 27 57139.0 28 68699.0 29 80884.0 30 96098.0 31 113290.0 32 135198.0 33 162314.0 34 196366.0 35 234516.0 36 257388.0 37 203818.0 38 60407.0 39 1408.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.361399718523074 21.731557357214346 16.308625699578556 21.598417224684017 2 17.433758627213873 22.75760184382729 37.0445051246727 22.764134404286136 3 20.817135120435346 25.332553302886986 30.479883247055774 23.370428329621898 4 15.64973569294415 19.173329785046388 35.3366316273331 29.840302894676363 5 14.245870472907484 37.71961361455618 33.77364525333621 14.260870659200123 6 31.50595119723118 37.432665272191244 16.730076105842716 14.33130742473486 7 27.64351556833583 33.314801616275396 20.834536927228413 18.207145888160362 8 26.37610095576242 35.42354650614232 19.369031323440396 18.83132121465486 9 26.1070891835686 16.701343090472566 20.083455668530807 37.10811205742803 10-11 25.00182655957947 27.107353801445473 27.099563706285352 20.791255932689705 12-13 26.01716434592684 24.328454763718074 27.733768288587846 21.92061260176724 14-15 23.03952094301763 25.63059852607529 25.99300730091589 25.33687322999119 16-17 22.228340324663183 28.736361584370258 26.65289771046145 22.382400380505114 18-19 22.2901019332774 27.228441639255102 29.17175945953008 21.309696967937423 20-21 22.993257613641834 26.297290634375596 29.402020562717933 21.307431189264634 22-23 22.76277902209629 26.21350086392639 29.313668423550858 21.710051690426457 24-25 22.610408371678687 26.42738713594494 29.291197838805072 21.6710066535713 26-27 22.556863037326096 26.518882005099652 29.195220870115584 21.72903408745867 28-29 22.455303298827644 26.64916582690863 29.24138698590779 21.654143888355932 30-31 22.68293509246577 26.43531267998277 29.126306160674098 21.75544606687736 32-33 22.59092874221379 26.376478221834702 29.17795370381347 21.85463933213804 34-35 22.691262426248166 26.52728929783971 29.026728606070666 21.75471966984146 36-37 22.885016109784626 26.447122257247468 28.948203824343828 21.71965780862408 38-39 22.626718410521683 26.583526485659952 28.985430104665173 21.804324999153195 40-41 22.766255095444006 26.43727329387308 28.88044917330897 21.916022437373943 42-43 22.74432838590565 26.5562840363984 28.660555750317123 22.03883182737883 44-45 22.787389024694725 26.623089444112185 28.54000820641455 22.049513324778534 46-47 23.06726998112203 26.87066230640281 28.125274441868402 21.936793270606756 48-49 23.119089778393683 26.980815602416502 27.84741222201228 22.052682397177538 50-51 22.998338627290497 27.11013326062358 27.69619839117573 22.19532972091019 52-53 23.171383157084897 27.228145556773285 27.44985326743069 22.150618018711125 54-55 23.071241402652586 27.197097206002713 27.450157824181904 22.281503567162797 56-57 23.049398913396644 27.386401272937665 27.396513937210997 22.167685876454694 58-59 23.15926304232857 27.169616681174407 27.47996456208482 22.191155714412208 60-61 23.070203048153687 27.13823043348987 27.698077128250887 22.09348939010556 62-63 22.910264130256227 27.1592298255236 27.764655845850918 22.16585019836926 64-65 22.876015606136193 27.128097383993115 27.64101144717735 22.35487556269334 66-67 22.91218039022712 27.21905415805096 27.480628116200577 22.388137335521343 68-69 22.865331148867234 27.126564198045788 27.622351230020215 22.385753423066763 70-71 23.001576935229526 27.063600473612826 27.464318150916426 22.470504440241218 72-73 23.020307510059876 27.231385992219217 27.252339279695658 22.49596721802525 74-75 22.995262562339004 27.39427379975114 27.041185779606202 22.569277858303654 76-77 23.041713804052346 27.267457445706928 27.14610186320497 22.54472688703576 78-79 22.94328858004902 27.40839045446077 27.041347389203562 22.606973576286645 80-81 23.138345099948083 27.25553932481518 27.017553876354018 22.588561698882724 82-83 23.046315989646 27.372394956936148 27.060028098742862 22.52126095467499 84-85 22.97277419829333 27.33191207693752 26.994274975371557 22.701038749397597 86-87 22.965127953522646 27.327678014561187 26.98878468225363 22.718409349662533 88-89 22.913374396988452 27.324612548446563 26.84588065088958 22.91613240367541 90-91 22.948350401482546 27.34864265271215 26.91967687852462 22.783330067280687 92-93 22.854145704541512 27.34013524305501 26.894381372254202 22.91133768014928 94-95 22.851877765261925 27.34316634344795 26.796234792269075 23.008721099021052 96-97 22.949966178324903 27.301470638044208 26.741187252083247 23.00737593154764 98-99 22.78517730726372 27.335109736524927 26.587815590187947 23.29189736602341 100-101 22.942257328970527 27.358643255895537 26.337673962875822 23.361425452258118 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 690.0 1 498.5 2 340.5 3 389.5 4 362.0 5 362.0 6 605.5 7 1289.5 8 2242.0 9 2733.5 10 2479.0 11 2064.0 12 2019.5 13 2236.5 14 2402.0 15 2539.5 16 2776.0 17 3055.5 18 3351.5 19 3665.5 20 4049.5 21 4647.5 22 5514.0 23 6763.0 24 8234.5 25 10213.5 26 13012.0 27 16117.5 28 18919.0 29 21711.5 30 24942.0 31 28248.5 32 31955.0 33 35317.0 34 38398.0 35 41790.0 36 46014.0 37 49921.0 38 52055.5 39 53680.0 40 55739.5 41 58679.0 42 61562.5 43 64732.5 44 68120.0 45 71246.0 46 73441.0 47 75121.5 48 77112.0 49 76556.5 50 75058.5 51 74799.0 52 75550.5 53 75438.0 54 73196.0 55 68709.0 56 62985.0 57 57569.5 58 52616.0 59 46702.5 60 39186.5 61 32194.0 62 25962.5 63 20411.5 64 15361.0 65 11189.0 66 8201.0 67 6187.5 68 4584.0 69 3375.5 70 2501.5 71 1771.0 72 1213.5 73 882.0 74 624.0 75 361.0 76 218.5 77 118.0 78 66.0 79 49.0 80 27.5 81 15.5 82 10.5 83 6.0 84 4.5 85 3.0 86 3.0 87 3.5 88 2.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023370285480375494 2 0.007548166739003265 3 0.006822381475637566 4 9.677136844875981E-5 5 0.004161168843296671 6 0.0 7 0.0 8 0.0 9 4.8385684224379905E-5 10-11 0.0 12-13 4.8385684224379905E-5 14-15 0.0 16-17 2.4192842112189952E-5 18-19 0.003919240422174772 20-21 0.0012338349477216874 22-23 0.0 24-25 0.00447567579075514 26-27 0.005636932212140258 28-29 0.007717516633788595 30-31 0.02695082611297961 32-33 6.048210528047487E-4 34-35 0.004040204632735722 36-37 0.013596377267050753 38-39 0.006628838738740046 40-41 0.008467494739266484 42-43 0.0047176042118770405 44-45 0.002467669895443375 46-47 0.001572534737292347 48-49 0.0010402922108241678 50-51 0.003024105264023744 52-53 0.002032198737423956 54-55 0.0013789920003948273 56-57 5.564353685803689E-4 58-59 6.773995791413186E-4 60-61 2.4192842112189952E-5 62-63 2.4192842112189952E-5 64-65 0.00503211115933551 66-67 3.628926316828492E-4 68-69 6.290138949169387E-4 70-71 0.002903141053462794 72-73 0.010910971792597667 74-75 0.00500791831722332 76-77 0.0035321549483797324 78-79 0.01025776505556854 80-81 1.9354273689751962E-4 82-83 0.004185361685408862 84-85 5.322425264681789E-4 86-87 0.0 88-89 8.225566318144584E-4 90-91 5.806282106925588E-4 92-93 1.6934989478532966E-4 94-95 7.257852633656984E-4 96-97 2.419284211218995E-4 98-99 1.9354273689751962E-4 100-101 7.015924212535085E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2066727.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.34300298088149 #Duplication Level Percentage of deduplicated Percentage of total 1 76.64632092958841 46.25069172327752 2 13.352008274789446 16.114005502527476 3 4.43795479263831 8.033985578435725 4 2.0059959272456562 4.841912728696831 5 1.027821015822596 3.101090331079777 6 0.6147387407432963 2.2257108999081634 7 0.3987530617916595 1.684337003743081 8 0.2803274110628928 1.3532638241112742 9 0.20303678761566646 1.1026664532288686 >10 0.9683056481184409 10.221422234771678 >50 0.04753380370623733 1.9523868357224634 >100 0.016239689534960123 1.6977819949441344 >500 4.8195867122795944E-4 0.1914135160466971 >1k 3.2130578081863374E-4 0.44298002612072385 >5k 1.6065289040931687E-4 0.7863513473855459 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8831 0.42729397738549885 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7392 0.3576669777866162 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4808 0.23263836975081859 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.83856842243799E-5 0.0 9 0.0 0.0 0.0 4.83856842243799E-5 0.0 10-11 0.0 0.0 0.0 4.83856842243799E-5 4.83856842243799E-5 12-13 0.0 0.0 0.0 4.83856842243799E-5 4.83856842243799E-5 14-15 0.0 0.0 0.0 4.83856842243799E-5 4.83856842243799E-5 16-17 0.0 0.0 0.0 7.257852633656985E-5 4.83856842243799E-5 18-19 0.0 0.0 0.0 1.451570526731397E-4 4.83856842243799E-5 20-21 0.0 0.0 0.0 1.6934989478532966E-4 7.257852633656985E-5 22-23 0.0 0.0 0.0 2.419284211218995E-4 9.67713684487598E-5 24-25 0.0 0.0 0.0 4.83856842243799E-4 9.67713684487598E-5 26-27 0.0 0.0 0.0 7.499781054778885E-4 9.67713684487598E-5 28-29 0.0 0.0 0.0 0.001475763368843587 9.67713684487598E-5 30-31 0.0 0.0 0.0 0.002951526737687174 1.2096421056094974E-4 32-33 0.0 0.0 0.0 0.005975632001710918 1.451570526731397E-4 34-35 0.0 0.0 0.0 0.00970132968698817 1.451570526731397E-4 36-37 0.0 0.0 0.0 0.016354361267840407 1.451570526731397E-4 38-39 0.0 0.0 0.0 0.026708897691857705 2.419284211218995E-4 40-41 0.0 4.83856842243799E-5 0.0 0.03863596885316735 3.386997895706593E-4 42-43 0.0 4.83856842243799E-5 0.0 0.05208718906754496 3.628926316828492E-4 44-45 0.0 4.83856842243799E-5 0.0 0.06919152844086326 4.354711580194191E-4 46-47 0.0 4.83856842243799E-5 0.0 0.09265858528968751 5.322425264681789E-4 48-49 0.0 4.83856842243799E-5 0.0 0.11503696424346321 5.806282106925588E-4 50-51 0.0 4.83856842243799E-5 0.0 0.14019752004014074 5.806282106925588E-4 52-53 0.0 4.83856842243799E-5 0.0 0.16545484720526707 5.806282106925588E-4 54-55 0.0 4.83856842243799E-5 0.0 0.1964216851088702 5.806282106925588E-4 56-57 0.0 4.83856842243799E-5 0.0 0.23677534575200304 6.048210528047488E-4 58-59 0.0 4.83856842243799E-5 0.0 0.28133856092265697 6.290138949169387E-4 60-61 0.0 4.83856842243799E-5 0.0 0.3249340624088232 6.290138949169387E-4 62-63 0.0 4.83856842243799E-5 0.0 0.37235203294871555 6.290138949169387E-4 64-65 0.0 4.83856842243799E-5 0.0 0.427052048964377 6.290138949169387E-4 66-67 0.0 4.83856842243799E-5 0.0 0.4919372515092705 6.290138949169387E-4 68-69 0.0 4.83856842243799E-5 0.0 0.5652657559513181 6.290138949169387E-4 70-71 0.0 4.83856842243799E-5 0.0 0.649311689449066 6.773995791413186E-4 72-73 0.0 4.83856842243799E-5 0.0 0.738704241053608 7.741709475900785E-4 74-75 0.0 4.83856842243799E-5 0.0 0.8342901602388704 8.225566318144583E-4 76-77 0.0 4.83856842243799E-5 0.0 0.9425047430066962 8.225566318144583E-4 78-79 0.0 4.83856842243799E-5 0.0 1.0684526790427569 8.225566318144583E-4 80-81 0.0 4.83856842243799E-5 0.0 1.2065696146612495 8.467494739266483E-4 82-83 0.0 4.83856842243799E-5 0.0 1.353879830282374 9.193280002632181E-4 84-85 0.0 4.83856842243799E-5 0.0 1.5052544433783464 9.193280002632181E-4 86-87 0.0 4.83856842243799E-5 0.0 1.6636933663710787 9.435208423754081E-4 88-89 0.0 4.83856842243799E-5 0.0 1.8404946565269626 9.67713684487598E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5395 0.0 47.827465 1 GTATCAA 8820 0.0 35.61245 1 ATCAACG 11225 0.0 27.632975 3 TATCAAC 11370 0.0 27.45034 2 TCAACGC 11265 0.0 27.44853 4 CAACGCA 11430 0.0 27.010082 5 AACGCAG 11880 0.0 25.985086 6 ACGCAGA 13390 0.0 22.770977 7 CGCAGAG 13510 0.0 22.428104 8 GAGTACT 8700 0.0 21.61817 12-13 GTACTTT 8895 0.0 20.852345 14-15 GCAGAGT 14470 0.0 20.349342 9 AGAGTAC 12225 0.0 20.279108 10-11 AGTACTT 8895 0.0 20.022963 12-13 GTGGTAT 2850 0.0 19.174408 1 CAGAGTA 13985 0.0 18.898615 10-11 GTACATG 6685 0.0 18.623823 1 ACTTTTT 10400 0.0 17.812794 16-17 TACTTTT 10000 0.0 17.716936 14-15 GGCGTAA 135 0.009381029 17.599697 1 >>END_MODULE