FastQCFastQC Report
Thu 26 May 2016
SRR936773_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936773_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146394
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99730.4646397632494314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71050.33102030661658577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46050.21454588486549997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44250.047.896041
GTATCAA80150.042.6881181
TCAACGC102050.033.389864
ATCAACG102000.033.313043
TATCAAC103000.033.220312
CAACGCA104450.032.5316475
AACGCAG105800.032.11136
ACGCAGA120650.027.8432257
CGCAGAG120800.027.4927028
GCAGAGT128750.025.2760989
GAGTACT75750.022.98571612-13
AGAGTAC111600.022.32790810-11
GTACTTT79450.021.5544814-15
AGTACTT79550.021.23078712-13
GTACATG69500.020.7858051
CAGAGTA126000.020.49253510-11
TACATGG70850.020.1228662
ACATGGG70400.018.9013923
TACTTTT90000.018.63195414-15
ACTTTTT96650.017.66945816-17
Produced by FastQC (version 0.11.5)