FastQCFastQC Report
Thu 26 May 2016
SRR936773_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936773_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2146394
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87620.40821955335320537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74990.3493766754845569No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48550.22619332704060857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56650.044.8678171
GTATCAA92250.036.308121
ATCAACG117900.028.279573
TATCAAC118350.028.214152
TCAACGC119650.027.8659534
CAACGCA121200.027.4369625
AACGCAG125300.026.501276
ACGCAGA143700.022.9095767
CGCAGAG142400.022.7517598
GGACCGT2303.6270649E-922.7198496
TGGACCG2951.3278623E-1020.9344675
GAGTACT89050.020.88081212-13
GCAGAGT153550.020.6355769
AGAGTAC130250.020.60683610-11
AGTACTT92500.020.1533612-13
GTACTTT93650.019.70347614-15
CAGAGTA148600.018.15808710-11
TACTTTT102700.017.92094414-15
ACTTTTT108200.017.36197316-17
GTACATG75350.017.2762071
Produced by FastQC (version 0.11.5)