##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936773_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2146394 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.40987675142588 31.0 31.0 34.0 30.0 34.0 2 31.583484206534308 31.0 31.0 34.0 30.0 34.0 3 31.65783122763109 31.0 31.0 34.0 30.0 34.0 4 35.45349036570173 37.0 35.0 37.0 33.0 37.0 5 35.29650520827024 37.0 35.0 37.0 33.0 37.0 6 35.36100827713831 37.0 35.0 37.0 33.0 37.0 7 35.29657882010479 37.0 35.0 37.0 32.0 37.0 8 35.2468181517466 37.0 35.0 37.0 32.0 37.0 9 36.788271864345504 39.0 37.0 39.0 32.0 39.0 10-11 36.63769280011032 38.0 35.0 39.0 32.0 39.0 12-13 36.493100754102 38.0 35.0 39.0 32.0 39.0 14-15 37.423557837004765 39.0 36.0 40.0 32.0 41.0 16-17 37.24977799975214 39.0 36.0 40.0 32.0 41.0 18-19 37.432164597925635 39.0 36.0 40.0 32.0 41.0 20-21 37.44828908392401 39.0 36.0 40.0 32.0 41.0 22-23 37.32235344489409 39.0 36.0 40.0 32.0 41.0 24-25 37.14544696826398 39.0 36.0 40.0 31.0 41.0 26-27 36.83609393242806 39.0 36.0 40.0 30.5 41.0 28-29 36.68200945399586 38.5 35.5 40.0 30.0 41.0 30-31 36.65035240501045 38.5 35.5 40.0 30.0 41.0 32-33 36.42170193356858 38.0 35.0 40.0 30.0 41.0 34-35 36.19319216322819 38.0 35.0 40.0 30.0 41.0 36-37 35.96661237405621 38.0 35.0 40.0 29.5 41.0 38-39 35.72801778238292 38.0 34.5 40.0 29.0 41.0 40-41 35.45916732901787 38.0 34.0 40.0 27.5 41.0 42-43 35.098097087487204 38.0 34.0 40.0 26.5 41.0 44-45 34.739748620244 38.0 33.0 40.0 25.5 41.0 46-47 34.29466141817392 38.0 33.0 40.0 24.0 41.0 48-49 33.818684733557774 37.0 33.0 40.0 23.5 41.0 50-51 33.40228028963928 37.0 32.0 39.5 22.5 40.5 52-53 33.027019969306664 37.0 31.0 39.0 20.5 40.0 54-55 32.61714182018772 36.0 31.0 39.0 19.0 40.0 56-57 32.163392415372016 36.0 30.5 39.0 17.5 40.0 58-59 31.645502875986423 35.0 30.0 38.5 14.0 40.0 60-61 31.145644741832115 35.0 29.0 38.0 12.5 40.0 62-63 30.60165375042979 34.0 28.0 38.0 8.0 40.0 64-65 30.04184972563285 34.0 28.0 37.5 7.5 39.5 66-67 29.452058662109565 34.0 27.0 37.0 7.0 39.0 68-69 28.961906108570933 33.0 26.0 36.5 2.0 39.0 70-71 28.322220431104448 32.5 26.0 36.0 2.0 39.0 72-73 27.641994433454435 32.0 25.0 35.5 2.0 38.0 74-75 26.941854803917643 31.5 23.5 35.0 2.0 37.5 76-77 25.98265602680589 30.5 23.0 34.0 2.0 36.0 78-79 26.6611791684099 31.5 24.5 34.5 2.0 36.0 80-81 26.340424917326455 31.5 23.5 34.5 2.0 36.0 82-83 25.796940822607592 31.0 21.5 34.0 2.0 36.0 84-85 25.199217618014217 31.0 20.0 34.0 2.0 35.0 86-87 24.550628169851386 31.0 18.5 34.0 2.0 35.0 88-89 23.835732395822948 30.0 14.0 34.0 2.0 35.0 90-91 22.999401088523356 29.5 4.5 34.0 2.0 35.0 92-93 22.06911312648097 29.0 2.0 34.0 2.0 35.0 94-95 20.948515976097585 28.5 2.0 33.0 2.0 34.5 96-97 19.68120088856007 26.5 2.0 33.0 2.0 34.0 98-99 18.223070181895775 25.0 2.0 32.0 2.0 34.0 100-101 14.881395959921619 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25009250605776145 1101 2 0.1511531317234578 1101 3 0.1708796744735963 1101 4 0.24293619061582916 1101 5 0.26362683869128034 1101 6 0.1847976351690832 1101 7 0.2719556126506646 1101 8 0.2480520281939107 1101 9 0.22503452792501832 1101 10-11 0.34680860293227767 1101 12-13 0.3145509761568448 1101 14-15 0.37730472581816343 1101 16-17 0.38204420180905885 1101 18-19 0.3627387099941046 1101 20-21 0.34432457830054375 1101 22-23 0.3607284182319077 1101 24-25 0.3572928417338588 1101 26-27 0.23672957806351747 1101 28-29 0.2156569967399662 1101 30-31 0.36629348370552606 1101 32-33 0.44403968801251636 1101 34-35 0.42061441217964557 1101 36-37 0.3631409470797351 1101 38-39 0.37509324824767276 1101 40-41 0.4521057856023063 1101 42-43 0.43724086793721995 1101 44-45 0.4420385242506697 1101 46-47 0.5683008565752345 1101 48-49 0.5527576677592805 1101 50-51 0.651692793312499 1101 52-53 0.7186740879061162 1101 54-55 0.6404297699119894 1101 56-57 0.6083870001669176 1101 58-59 0.6602729696895331 1101 60-61 0.7851217798199421 1101 62-63 0.7203860041366603 1101 64-65 0.7862903721838279 1101 66-67 0.37523127041588467 1101 68-69 0.5541456426436113 1101 70-71 0.6119324521184488 1101 72-73 0.6607945542312947 1101 74-75 0.7540988769085857 1101 76-77 0.4399434909577131 1101 78-79 0.5418920614464575 1101 80-81 0.973531613822054 1101 82-83 0.8718845320953434 1101 84-85 0.7549331260126451 1101 86-87 0.40859010805753115 1101 88-89 0.4308999552112702 1101 90-91 0.7074533295358592 1101 92-93 0.6784600239845169 1101 94-95 0.42773467806397747 1101 96-97 0.4775824050577633 1101 98-99 0.4837457705065198 1101 100-101 0.7366413829215954 1102 1 0.2713003265003415 1102 2 0.33972692145729155 1102 3 0.38015045876626985 1102 4 0.32377745534408575 1102 5 0.30106178381620907 1102 6 0.1826004988225094 1102 7 0.17016784824406983 1102 8 0.2410876806974045 1102 9 0.3364708560539569 1102 10-11 0.37981044433741573 1102 12-13 0.34342451159047016 1102 14-15 0.46460593619904245 1102 16-17 0.48470081760650885 1102 18-19 0.38177899055945375 1102 20-21 0.4241797529105398 1102 22-23 0.3904112679727021 1102 24-25 0.5774659673137492 1102 26-27 0.36528924179256705 1102 28-29 0.3926021271800906 1102 30-31 0.5288701018523341 1102 32-33 0.4349840861121024 1102 34-35 0.5483324119722468 1102 36-37 0.41199645849295763 1102 38-39 0.5576736955810944 1102 40-41 0.5726294916859658 1102 42-43 0.6043827414809115 1102 44-45 0.5797491180430328 1102 46-47 0.7074293850938389 1102 48-49 0.83958594262009 1102 50-51 0.8257734902657603 1102 52-53 0.8596201034871669 1102 54-55 0.9231457989009186 1102 56-57 0.8065897373585322 1102 58-59 0.8261368034463921 1102 60-61 0.97474859587307 1102 62-63 1.1045819598364837 1102 64-65 0.8686683590182156 1102 66-67 0.9237324845594159 1102 68-69 0.6636915249717177 1102 70-71 0.6398068772815968 1102 72-73 0.7694170955447532 1102 74-75 0.9085840727087309 1102 76-77 0.6622643107202606 1102 78-79 0.6889321965531927 1102 80-81 0.903816040481086 1102 82-83 0.7631584604966619 1102 84-85 0.7106704589531923 1102 86-87 0.7748919007317383 1102 88-89 0.7480066800797367 1102 90-91 0.9369674105723682 1102 92-93 0.9603210820972983 1102 94-95 0.8309189484352935 1102 96-97 1.233005613920735 1102 98-99 1.2276579603056952 1102 100-101 1.166336805426111 1103 1 0.2240489512321382 1103 2 0.2730624850782952 1103 3 0.18787110021521158 1103 4 0.3140003748725775 1103 5 0.19369368712550283 1103 6 0.2659742276723307 1103 7 0.05746435725215804 1103 8 0.15346354698210263 1103 9 0.246183007844607 1103 10-11 0.2790845653537417 1103 12-13 0.38588714061716445 1103 14-15 0.3794491151145394 1103 16-17 0.4198285766771832 1103 18-19 0.45579689021107583 1103 20-21 0.3246472485159586 1103 22-23 0.24249847586191464 1103 24-25 0.07082136001278627 1103 26-27 0.2506042653744416 1103 28-29 0.3628828612269359 1103 30-31 0.3522766068276937 1103 32-33 0.31792994109970607 1103 34-35 0.2764878252445442 1103 36-37 0.15729100666853668 1103 38-39 0.0892485950133306 1103 40-41 0.4252789368905283 1103 42-43 0.40577716057290303 1103 44-45 0.2737415309685005 1103 46-47 0.3748248779100365 1103 48-49 0.4645112393621389 1103 50-51 0.4460262774426056 1103 52-53 0.4433152327209555 1103 54-55 0.3018791411554105 1103 56-57 0.5291906533240187 1103 58-59 0.4536485511453314 1103 60-61 0.5126391920699298 1103 62-63 0.48406921897678856 1103 64-65 0.4890731698852697 1103 66-67 0.4789551469220825 1103 68-69 0.27530564560570525 1103 70-71 0.29119100974065404 1103 72-73 0.4731258249817287 1103 74-75 0.5796053760524345 1103 76-77 0.4381008847981285 1103 78-79 0.6675220607605326 1103 80-81 0.7591241365163626 1103 82-83 0.6493533667016891 1103 84-85 0.6410337090922447 1103 86-87 0.5526111334581572 1103 88-89 0.4106187178981351 1103 90-91 0.37503357364918344 1103 92-93 0.3930074726998676 1103 94-95 0.22155423222347181 1103 96-97 0.2677323877504456 1103 98-99 -0.014134922436699782 1103 100-101 0.3829090034792966 1104 1 0.22834191449030783 1104 2 0.1680935838282558 1104 3 0.18608819164463242 1104 4 0.1346708864699906 1104 5 0.11163023581362097 1104 6 0.05323896140975393 1104 7 0.15708603755001604 1104 8 0.20584098605264955 1104 9 0.2267444081692176 1104 10-11 0.26393238901999183 1104 12-13 0.29248636781895954 1104 14-15 0.17016200537103998 1104 16-17 0.32179569096737026 1104 18-19 0.2731633549544483 1104 20-21 0.20936808205089363 1104 22-23 0.25401318534955664 1104 24-25 0.2602922911834682 1104 26-27 0.06664497907495104 1104 28-29 0.17394206842237026 1104 30-31 0.34104710715098463 1104 32-33 0.42548731816027185 1104 34-35 0.42585228425917876 1104 36-37 0.39132666530296234 1104 38-39 0.43517302321048845 1104 40-41 0.4190544411180994 1104 42-43 0.43001941574910063 1104 44-45 0.22860134872591686 1104 46-47 0.33383435951574114 1104 48-49 0.30536636412175255 1104 50-51 0.4303616726052013 1104 52-53 0.2926591112549133 1104 54-55 0.2132979974594349 1104 56-57 0.14898606621499155 1104 58-59 0.07744454093796804 1104 60-61 0.2564162010570463 1104 62-63 0.2300240201668018 1104 64-65 0.17228991521999504 1104 66-67 0.11901307321988597 1104 68-69 0.114914655828116 1104 70-71 0.015981541423137458 1104 72-73 0.28306913721679194 1104 74-75 0.40160239107247975 1104 76-77 0.22090284894919776 1104 78-79 0.25198386730077615 1104 80-81 0.6474396091254526 1104 82-83 0.5912409995922125 1104 84-85 0.4851767334783794 1104 86-87 0.34396829230544057 1104 88-89 0.3175476610684598 1104 90-91 0.3467723950798316 1104 92-93 0.41924087201883964 1104 94-95 0.40712760464660036 1104 96-97 0.37988252247273024 1104 98-99 0.47770441924719975 1104 100-101 0.39015800900685704 1105 1 0.1061725230950259 1105 2 0.18922716029235787 1105 3 0.1092806098282395 1105 4 0.09147837958250449 1105 5 0.14751723889632729 1105 6 0.10871338302965228 1105 7 0.04761275873178761 1105 8 0.10712761120754521 1105 9 0.1427200264291102 1105 10-11 0.1268386312353087 1105 12-13 0.060925502295766876 1105 14-15 0.13535084484584559 1105 16-17 0.019728668778839165 1105 18-19 0.0396345568698564 1105 20-21 0.18353307669046615 1105 22-23 0.11396706672339718 1105 24-25 0.02117071339767307 1105 26-27 0.122059525575132 1105 28-29 0.1914724809175965 1105 30-31 0.20557971038567047 1105 32-33 0.009211893102367696 1105 34-35 0.12857705804580633 1105 36-37 0.11557186303499378 1105 38-39 0.16861736780830938 1105 40-41 0.03580722857595475 1105 42-43 0.25412984518184345 1105 44-45 0.2886173474564657 1105 46-47 0.3358578552026401 1105 48-49 0.2543531317202934 1105 50-51 0.2828153944352536 1105 52-53 0.222367876701405 1105 54-55 0.2623904212363186 1105 56-57 0.2929557915803862 1105 58-59 0.18790465043305105 1105 60-61 0.13457630761997663 1105 62-63 0.16276996954103495 1105 64-65 -0.19778132138849358 1105 66-67 -0.20471469752591887 1105 68-69 -0.06376877899313627 1105 70-71 -0.21198342892143174 1105 72-73 -0.13542148247098496 1105 74-75 -0.038969951008123616 1105 76-77 -0.1460753326064541 1105 78-79 0.1549584533924495 1105 80-81 0.057243408460522005 1105 82-83 0.008759510983392005 1105 84-85 0.08010676459619148 1105 86-87 0.2637460422674316 1105 88-89 -0.04176644356318704 1105 90-91 -0.2635911873446801 1105 92-93 -0.3735958953456233 1105 94-95 -0.25489145091825094 1105 96-97 -0.2773504659092829 1105 98-99 -0.3841518523759113 1105 100-101 -0.2730481385663399 1106 1 0.25216361156416767 1106 2 0.1741803080787001 1106 3 0.18130222028388587 1106 4 0.1251493495344107 1106 5 0.18253003452565508 1106 6 0.17205352045710498 1106 7 0.212586159632572 1106 8 0.17380920488234608 1106 9 0.22384162790982742 1106 10-11 0.10288867469885332 1106 12-13 0.24410931810183456 1106 14-15 0.21201795156498804 1106 16-17 0.3327933052653904 1106 18-19 0.05987088511476202 1106 20-21 0.2426010936896006 1106 22-23 0.16512657825711585 1106 24-25 0.13181535550460666 1106 26-27 0.10159140068957129 1106 28-29 0.37494132767646704 1106 30-31 0.3576251318268717 1106 32-33 0.32918809549226324 1106 34-35 0.05495407120230311 1106 36-37 0.19516919762776297 1106 38-39 0.26143613935031595 1106 40-41 0.40480855421733253 1106 42-43 0.1272098152903709 1106 44-45 -0.016409509789035326 1106 46-47 0.21703880357859617 1106 48-49 0.1998878095276453 1106 50-51 0.12948586240631954 1106 52-53 -0.047566600955633476 1106 54-55 -0.11012731160795397 1106 56-57 0.02007965003543788 1106 58-59 0.03977556230872992 1106 60-61 -0.08901174922196375 1106 62-63 0.05989559756881846 1106 64-65 -0.20721924329215113 1106 66-67 -0.20252272790853354 1106 68-69 -0.3397022821145015 1106 70-71 -0.4502131285581932 1106 72-73 -0.06720853291691498 1106 74-75 0.09314992089246488 1106 76-77 0.03309604723627757 1106 78-79 0.13822355184048263 1106 80-81 0.08067553424437435 1106 82-83 0.26128904365434735 1106 84-85 0.14859190466596672 1106 86-87 -0.049356112371288674 1106 88-89 0.03368128977196605 1106 90-91 -0.10318684198025707 1106 92-93 0.18073319783461983 1106 94-95 0.1838104424175775 1106 96-97 0.04309213860753047 1106 98-99 0.22563114289947706 1106 100-101 2.2104073310735828E-4 1107 1 0.09248743012395266 1107 2 0.1594472578359145 1107 3 0.13815598126135598 1107 4 0.1489690663667247 1107 5 0.1886585206083211 1107 6 0.03729624938826248 1107 7 0.17517115429087227 1107 8 0.07141064887554194 1107 9 0.055753723281256384 1107 10-11 0.2216690712570184 1107 12-13 0.18124894653585244 1107 14-15 0.21815993651567567 1107 16-17 0.14899271825747462 1107 18-19 0.08061093162800148 1107 20-21 0.0547662735523744 1107 22-23 0.2661656054129864 1107 24-25 0.21416809249651436 1107 26-27 0.32157666763933435 1107 28-29 0.38871402987987835 1107 30-31 0.23087654631540744 1107 32-33 0.07747470345813667 1107 34-35 0.18287512982210785 1107 36-37 0.29783773099503463 1107 38-39 0.3143748429190296 1107 40-41 0.3551288630714353 1107 42-43 0.33626292755081266 1107 44-45 0.11337824943743868 1107 46-47 0.030224112182004603 1107 48-49 0.058485238571812204 1107 50-51 0.1440549978231047 1107 52-53 0.013160949462637461 1107 54-55 0.16084125145683004 1107 56-57 0.025170651690007873 1107 58-59 0.06689093134849955 1107 60-61 0.1526179883986103 1107 62-63 0.21099999781410972 1107 64-65 0.18322008700972248 1107 66-67 0.27753944402076414 1107 68-69 0.38699577906236726 1107 70-71 0.39573971796739116 1107 72-73 0.030153016907405572 1107 74-75 -0.06817697926604893 1107 76-77 0.10210997421125612 1107 78-79 0.4001845131141586 1107 80-81 0.49498086321178647 1107 82-83 0.44369539043641026 1107 84-85 0.5569288055572805 1107 86-87 0.31345682031564337 1107 88-89 0.3244025535745543 1107 90-91 0.4241541509964719 1107 92-93 0.3817756961279777 1107 94-95 0.3856532307437526 1107 96-97 0.4655970656943573 1107 98-99 0.2856220408056096 1107 100-101 0.19442539816770754 1108 1 0.09554021073470054 1108 2 0.11422214257160235 1108 3 0.04555861016601881 1108 4 0.15732962578832144 1108 5 -0.010719727920239563 1108 6 0.024736978888142858 1108 7 0.08793435308432862 1108 8 -0.019177475589437165 1108 9 0.07349607642535005 1108 10-11 0.006620291845912618 1108 12-13 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-0.7083513252476621 2316 88-89 -1.1028504654700804 2316 90-91 -0.6994894698387846 2316 92-93 -0.46116405045108877 2316 94-95 -0.6702221844984457 2316 96-97 0.03434403012560594 2316 98-99 -0.17061811480435907 2316 100-101 -0.05191200186876266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 40.0 5 89.0 6 274.0 7 628.0 8 1526.0 9 2479.0 10 4148.0 11 6204.0 12 8979.0 13 12098.0 14 15717.0 15 20486.0 16 25955.0 17 31447.0 18 30261.0 19 26072.0 20 26416.0 21 28409.0 22 30816.0 23 33998.0 24 38683.0 25 45973.0 26 53671.0 27 63790.0 28 75292.0 29 88625.0 30 104285.0 31 123306.0 32 144373.0 33 172340.0 34 205643.0 35 239367.0 36 252229.0 37 184573.0 38 47200.0 39 987.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.394173836132616 21.71768008477538 16.273965402025997 21.614180677066003 2 17.51112846398776 22.73412172960139 36.96341328184623 22.791336524564624 3 20.850674457729276 25.26883498859248 30.340371348929434 23.54011920474881 4 15.568699071464179 19.089913762177424 35.43452028755259 29.90686687880581 5 14.289654452681525 37.655982690953 33.783623867401666 14.270738988963805 6 31.604588905857916 37.40338446715747 16.690877816467992 14.301148810516615 7 27.695334593741876 33.269893598286245 20.83568999913343 18.199081808838454 8 26.367293236935996 35.455932135479316 19.36834523391325 18.80842939367143 9 26.101172478119118 16.687849481502464 20.08988098177688 37.12109705860154 10-11 24.984600474333345 27.091213650363205 27.125437407101956 20.798748468201495 12-13 25.977383462682063 24.278697200979877 27.715950566391818 22.027968769946245 14-15 23.020191073959396 25.58456182788435 26.010159364962814 25.38508773319344 16-17 22.26876798947444 28.68492457582345 26.632086187344918 22.414221247357197 18-19 22.325957365523895 27.208795253414465 29.104401120954538 21.36084626010711 20-21 23.06666247354548 26.258210073041845 29.36125952042341 21.313867932989265 22-23 22.76523881084826 26.208474046796116 29.234280373666316 21.792006768689316 24-25 22.717465678028 26.388750076589446 29.177747382737778 21.716036862644785 26-27 22.549850507881825 26.5442277219004 29.152454005023603 21.753467765194173 28-29 22.46943351781389 26.648945413851276 29.168062423039277 21.713558645295556 30-31 22.724928354747316 26.398649048449474 29.068846999472125 21.807575597331084 32-33 22.597225791016236 26.385945523598576 29.085561212045537 21.931267473339652 34-35 22.7688625667049 26.530473901809266 28.960293403727366 21.740370127758464 36-37 22.92103482787348 26.444079149683258 28.871588640823926 21.76329738161934 38-39 22.730976016558206 26.51914973325198 28.942748714952266 21.807125535237553 40-41 22.824835856776957 26.405225560800837 28.85123621365816 21.91870236876404 42-43 22.781302034947917 26.532011364176384 28.610730369168007 22.075956231707693 44-45 22.884148949354127 26.639191127071733 28.45724037618443 22.019419547389717 46-47 23.118235735350982 26.827836506878995 28.106914400458443 21.94701335731158 48-49 23.206539527309957 26.963599535486697 27.80025927949188 22.029601657711467 50-51 23.05202111763563 27.13677214768181 27.63538298894404 22.175823745738523 52-53 23.249785454115134 27.182078406853545 27.409646132070815 22.15849000696051 54-55 23.125198740639537 27.129853202738165 27.398917453184566 22.346030603437733 56-57 23.1123430840017 27.375174804078494 27.33806497272206 22.174417139197743 58-59 23.191677555111763 27.167635309264842 27.465414578954366 22.17527255666903 60-61 23.088330599243275 27.14713815082952 27.636339155135886 22.128192094791313 62-63 22.95121111998168 27.134413037142785 27.698104434215466 22.21627140866007 64-65 22.90215394019359 27.09740345659134 27.64520646741136 22.35523613580371 66-67 22.948767018044258 27.223140183981126 27.402187376171256 22.425905421803364 68-69 22.885948799899367 27.094471744901018 27.59363720119783 22.42594225400179 70-71 23.020672718873804 27.072123079818827 27.3912418563446 22.515962344962762 72-73 23.119077858026067 27.206934048455224 27.190371385682216 22.483616707836493 74-75 23.026701074779876 27.3266591905583 26.97989138056265 22.666748354099177 76-77 23.06682743242853 27.26244575786179 27.108047264388553 22.562679545321128 78-79 23.01597562575025 27.277252897750078 27.01951463486963 22.68725684163004 80-81 23.054226369391763 27.23632320434074 27.052500659836976 22.65694976643052 82-83 23.047538417883505 27.369477509329275 26.953370164304275 22.62961390848294 84-85 23.033746081563127 27.332681347681365 26.925229142360863 22.70834342839464 86-87 22.95966776862238 27.384209039080798 26.90925999220532 22.746863200091504 88-89 22.901399257403774 27.273813664208507 26.876471803683256 22.948315274704463 90-91 23.00676393413294 27.35032501174068 26.832337550037643 22.810573504088737 92-93 22.89481408318153 27.349573003974115 26.81686459590289 22.938748316941467 94-95 22.875674940312667 27.303008511719774 26.744443644653227 23.076872903314328 96-97 22.9501716047637 27.33776984391922 26.686084068513317 23.025974482803765 98-99 22.781953958913334 27.309537914208924 26.54137890308058 23.36712922379716 100-101 23.04490148099971 27.309624354962846 26.255923905649865 23.38955025838758 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 672.0 1 483.0 2 324.5 3 367.5 4 357.5 5 383.5 6 649.5 7 1332.0 8 2216.0 9 2748.0 10 2480.0 11 2078.0 12 2077.0 13 2209.5 14 2450.5 15 2689.5 16 2924.5 17 3173.5 18 3389.5 19 3644.0 20 4146.5 21 4824.0 22 5669.0 23 6864.0 24 8480.5 25 10613.0 26 13402.5 27 16474.5 28 19440.5 29 22567.0 30 25604.5 31 28915.0 32 32828.0 33 36490.0 34 39779.5 35 43071.5 36 47351.0 37 51488.5 38 54154.0 39 55558.5 40 57582.0 41 60991.0 42 64260.5 43 67589.0 44 70585.5 45 73500.5 46 75694.5 47 77570.5 48 79742.5 49 80089.0 50 78622.5 51 77814.0 52 78545.5 53 78648.0 54 76491.5 55 71775.5 56 65727.0 57 59818.0 58 54786.5 59 48821.0 60 41183.5 61 33872.5 62 27342.5 63 21593.5 64 16296.5 65 11744.0 66 8564.5 67 6523.5 68 4819.5 69 3552.5 70 2647.5 71 1849.5 72 1269.0 73 915.0 74 633.5 75 350.0 76 215.5 77 134.0 78 80.0 79 39.5 80 23.0 81 16.0 82 12.0 83 8.0 84 6.0 85 4.5 86 4.0 87 3.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022735807125811944 2 0.004472617795241694 3 1.3976930610130292E-4 4 1.8635907480173724E-4 5 9.317953740086862E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.010436108188897286 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.1930791830390883E-4 20-21 0.0010016800270593376 22-23 0.0018868856323675895 24-25 0.009807146311441422 26-27 0.005916900624955157 28-29 3.261283809030402E-4 30-31 0.002562437278523887 32-33 0.004123194529988436 34-35 0.0 36-37 0.008479337903479043 38-39 0.012462763127366177 40-41 0.0010016800270593376 42-43 0.0 44-45 0.0 46-47 1.8635907480173724E-4 48-49 0.00286527077507671 50-51 0.0061032596997568946 52-53 0.0 54-55 0.0030982196185788814 56-57 0.0029118605437771445 58-59 2.5624372785238873E-4 60-61 0.0017238214419160694 62-63 0.004449322910891476 64-65 9.317953740086862E-5 66-67 0.001514167482764115 68-69 5.357823400549945E-4 70-71 6.755516461562975E-4 72-73 0.0 74-75 4.658976870043431E-5 76-77 0.0 78-79 0.0014209879453632465 80-81 0.0014209879453632465 82-83 0.008991825359183822 84-85 0.00605666993105646 86-87 0.002725501468975407 88-89 2.5624372785238873E-4 90-91 4.6589768700434306E-4 92-93 1.8635907480173724E-4 94-95 2.3294884350217155E-5 96-97 0.0017471163262662865 98-99 0.0013278084079623778 100-101 2.7953861220260585E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2146394.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.055044795373604 #Duplication Level Percentage of deduplicated Percentage of total 1 76.56577969387564 45.981613293084074 2 13.391400235811538 16.084422820688772 3 4.454970612977656 8.026303791733383 4 1.9984962981459267 4.800791388341677 5 1.034493172096764 3.106326689538965 6 0.6088299023783151 2.1937984236055588 7 0.40084459714324633 1.68509181661948 8 0.28423074527686853 1.3655592111859813 9 0.1935325700548941 1.0460346447609399 >10 1.0019454077520455 10.619285466016319 >50 0.04753609931103151 1.9453011447712887 >100 0.01700965892802595 1.7692533286558731 >500 4.655031240839893E-4 0.19009951459089716 >1k 3.103354160559568E-4 0.4284693961219937 >5k 1.551677080279784E-4 0.7576490702848782 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8762 0.40821955335320537 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7499 0.3493766754845569 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4855 0.22619332704060857 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 4.658976870043431E-5 12-13 0.0 0.0 0.0 0.0 4.658976870043431E-5 14-15 0.0 0.0 0.0 0.0 6.988465305065146E-5 16-17 0.0 0.0 0.0 0.0 9.317953740086862E-5 18-19 0.0 0.0 0.0 0.0 9.317953740086862E-5 20-21 0.0 0.0 0.0 4.658976870043431E-5 2.3294884350217156E-4 22-23 0.0 0.0 0.0 1.8635907480173724E-4 2.3294884350217156E-4 24-25 0.0 0.0 0.0 3.261283809030402E-4 2.3294884350217156E-4 26-27 0.0 0.0 0.0 6.289618774558631E-4 3.261283809030402E-4 28-29 0.0 0.0 0.0 0.001211333986211292 3.261283809030402E-4 30-31 0.0 0.0 0.0 0.002422667972422584 3.261283809030402E-4 32-33 0.0 0.0 0.0 0.004589092216992779 3.727181496034745E-4 34-35 0.0 0.0 0.0 0.008199799291276438 3.727181496034745E-4 36-37 0.0 0.0 0.0 0.01448941806583507 3.727181496034745E-4 38-39 0.0 0.0 0.0 0.022223319670107166 3.960130339536916E-4 40-41 0.0 0.0 0.0 0.031727632484995764 4.193079183039088E-4 42-43 0.0 0.0 0.0 0.04169784298688871 4.193079183039088E-4 44-45 0.0 0.0 0.0 0.05576795313441987 4.4260280265412595E-4 46-47 0.0 0.0 0.0 0.07505611737639967 4.891925713545603E-4 48-49 0.0 0.0 0.0 0.09357555043482232 5.590772244052117E-4 50-51 0.0 0.0 0.0 0.11537956218662557 5.590772244052117E-4 52-53 0.0 0.0 0.0 0.13790571535328555 5.590772244052117E-4 54-55 0.0 0.0 0.0 0.16432211420643184 5.590772244052117E-4 56-57 0.0 0.0 0.0 0.19837923512644928 5.823721087554289E-4 58-59 4.658976870043431E-5 0.0 0.0 0.23774758967831627 6.05666993105646E-4 60-61 4.658976870043431E-5 0.0 0.0 0.2787232912503482 6.05666993105646E-4 62-63 4.658976870043431E-5 0.0 0.0 0.32468409807332665 6.05666993105646E-4 64-65 4.658976870043431E-5 0.0 0.0 0.37467491988889273 6.522567618060804E-4 66-67 4.658976870043431E-5 0.0 0.0 0.4313047837442706 6.522567618060804E-4 68-69 4.658976870043431E-5 0.0 0.0 0.4979048581015415 6.522567618060804E-4 70-71 4.658976870043431E-5 0.0 0.0 0.5743120787702538 6.755516461562975E-4 72-73 4.658976870043431E-5 0.0 0.0 0.6552385070029081 6.988465305065146E-4 74-75 4.658976870043431E-5 0.0 0.0 0.7440619010302862 7.687311835571661E-4 76-77 4.658976870043431E-5 0.0 0.0 0.8449054553823763 7.920260679073833E-4 78-79 4.658976870043431E-5 0.0 0.0 0.9580254137870308 7.920260679073833E-4 80-81 4.658976870043431E-5 0.0 0.0 1.0804633259317722 7.920260679073833E-4 82-83 4.658976870043431E-5 0.0 0.0 1.2157367193534832 8.386158366078175E-4 84-85 4.658976870043431E-5 0.0 0.0 1.3582548218081116 8.386158366078175E-4 86-87 4.658976870043431E-5 0.0 0.0 1.504197272262222 8.386158366078175E-4 88-89 4.658976870043431E-5 0.0 0.0 1.6712448879376294 8.386158366078175E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5665 0.0 44.867817 1 GTATCAA 9225 0.0 36.30812 1 ATCAACG 11790 0.0 28.27957 3 TATCAAC 11835 0.0 28.21415 2 TCAACGC 11965 0.0 27.865953 4 CAACGCA 12120 0.0 27.436962 5 AACGCAG 12530 0.0 26.50127 6 ACGCAGA 14370 0.0 22.909576 7 CGCAGAG 14240 0.0 22.751759 8 GGACCGT 230 3.6270649E-9 22.719849 6 TGGACCG 295 1.3278623E-10 20.934467 5 GAGTACT 8905 0.0 20.880812 12-13 GCAGAGT 15355 0.0 20.635576 9 AGAGTAC 13025 0.0 20.606836 10-11 AGTACTT 9250 0.0 20.15336 12-13 GTACTTT 9365 0.0 19.703476 14-15 CAGAGTA 14860 0.0 18.158087 10-11 TACTTTT 10270 0.0 17.920944 14-15 ACTTTTT 10820 0.0 17.361973 16-17 GTACATG 7535 0.0 17.276207 1 >>END_MODULE