FastQCFastQC Report
Thu 26 May 2016
SRR936772_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936772_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133610
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100820.47253246844549845No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70830.3319725723070289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46640.21859665074685625No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47400.046.6281361
GTATCAA81450.038.864811
ATCAACG99500.031.4367313
TCAACGC99900.031.3108584
TATCAAC100900.031.236112
CAACGCA101150.030.9239235
AACGCAG104150.029.984016
ACGCAGA117450.026.2605637
CGCAGAG119000.025.5590888
GCAGAGT128400.023.0941169
GAGTACT75000.021.89063512-13
GTGGTAT24300.021.5159171
GTACATG69950.021.4040661
AGAGTAC110000.020.88696510-11
GTACCGT1856.5186596E-620.5542626
TACATGG72550.020.3778112
GTACTTT80700.020.1970114-15
CAGAGTA124500.019.7520710-11
AGTACTT79650.019.50893412-13
ACATGGG70950.018.7603193
Produced by FastQC (version 0.11.5)