FastQCFastQC Report
Thu 26 May 2016
SRR936772_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936772_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2133610
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88110.4129620689816789No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74020.3469237583250922No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48060.22525203762637033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58350.045.9191631
GTATCAA91600.034.3335151
TATCAAC116400.026.76922
ATCAACG118150.026.331883
TCAACGC118950.026.1947174
CAACGCA121100.025.8081045
AACGCAG124550.024.982336
ACGCAGA140350.021.9329977
GAGTACT85850.021.60731912-13
CGCAGAG141600.021.572658
GTACTTT88550.020.73220314-15
AGAGTAC125400.020.09757210-11
GCAGAGT148900.019.9407319
AGTACTT88800.019.7393812-13
TAAGGTG8750.019.5419885
CAGAGTA146250.018.17439510-11
GTGGTAT32500.018.125671
TGGTATC31350.018.0299242
ACTTTTT105000.017.86867316-17
GCGGTAT2152.393039E-517.6769491
Produced by FastQC (version 0.11.5)