##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936772_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2133610 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.31068002118475 31.0 31.0 33.0 30.0 34.0 2 31.483889745548623 31.0 31.0 34.0 30.0 34.0 3 31.570094347139356 31.0 31.0 34.0 30.0 34.0 4 35.39416294449314 37.0 35.0 37.0 33.0 37.0 5 35.12159532435637 37.0 35.0 37.0 32.0 37.0 6 35.25814605293376 37.0 35.0 37.0 32.0 37.0 7 35.2070884557159 37.0 35.0 37.0 32.0 37.0 8 35.15487835171376 37.0 35.0 37.0 32.0 37.0 9 36.70421445343807 38.0 35.0 39.0 32.0 39.0 10-11 36.53637075191811 38.0 35.0 39.0 32.0 39.0 12-13 36.38536166403419 38.0 35.0 39.0 32.0 39.0 14-15 37.30196169871719 39.0 36.0 40.0 32.0 41.0 16-17 37.259177403555476 39.0 36.0 40.0 32.0 41.0 18-19 37.390274698750005 39.0 36.0 40.0 32.0 41.0 20-21 37.37243544977761 39.0 36.0 40.0 32.0 41.0 22-23 37.22910325692136 39.0 36.0 40.0 31.0 41.0 24-25 37.08603399871579 39.0 36.0 40.0 31.0 41.0 26-27 36.77461415160221 39.0 36.0 40.0 30.5 41.0 28-29 36.53745600179977 38.0 35.0 40.0 30.0 41.0 30-31 36.48803858249633 38.0 35.0 40.0 30.0 41.0 32-33 36.312958319467946 38.0 35.0 40.0 30.0 41.0 34-35 36.11300261059894 38.0 35.0 40.0 30.0 41.0 36-37 35.89381166192509 38.0 35.0 40.0 29.0 41.0 38-39 35.64814235028895 38.0 34.0 40.0 28.5 41.0 40-41 35.20129475396159 38.0 34.0 40.0 26.5 41.0 42-43 34.91131837589812 38.0 33.0 40.0 26.0 41.0 44-45 34.618126086773124 38.0 33.0 40.0 25.5 41.0 46-47 34.17399641921438 37.5 33.0 40.0 24.0 41.0 48-49 33.71172074559081 37.0 32.5 40.0 23.0 41.0 50-51 33.31227801706966 37.0 32.0 39.5 22.0 40.0 52-53 32.92826031936483 36.5 31.0 39.0 20.5 40.0 54-55 32.40267574673909 36.0 31.0 39.0 18.0 40.0 56-57 32.05444692328963 36.0 30.0 39.0 17.5 40.0 58-59 31.598996770731297 35.0 29.5 38.5 14.0 40.0 60-61 31.056984406709756 35.0 29.0 38.0 12.0 40.0 62-63 30.533163511607086 34.0 28.0 38.0 8.0 40.0 64-65 30.06824396211116 34.0 28.0 38.0 8.0 39.5 66-67 29.539264907832266 34.0 27.5 37.0 7.0 39.0 68-69 28.926398920140045 33.0 26.0 36.5 3.5 39.0 70-71 28.3448148911938 33.0 26.0 36.0 2.0 39.0 72-73 27.78304962012739 32.0 25.5 35.5 2.0 38.0 74-75 27.17140737998041 32.0 24.5 35.0 2.0 37.5 76-77 26.158884707139542 30.5 23.0 34.0 2.0 36.0 78-79 26.794841372134552 31.5 25.0 34.5 2.0 36.0 80-81 26.563798679233784 32.0 24.5 34.5 2.0 36.0 82-83 26.04801486682196 31.0 23.5 34.0 2.0 36.0 84-85 25.39049381095889 31.0 20.5 34.0 2.0 35.0 86-87 24.756417058412737 31.0 19.0 34.0 2.0 35.0 88-89 24.09486410356157 30.0 17.0 34.0 2.0 35.0 90-91 23.284646209944647 30.0 8.5 34.0 2.0 35.0 92-93 22.38734773459067 29.0 2.0 34.0 2.0 35.0 94-95 21.344478841025307 29.0 2.0 33.5 2.0 34.5 96-97 20.02810073068649 27.0 2.0 33.0 2.0 34.0 98-99 18.56532121615478 25.5 2.0 32.0 2.0 34.0 100-101 15.441355964773319 10.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16557458298190042 1101 2 0.24914502981400943 1101 3 0.185789439886495 1101 4 0.15312153255725036 1101 5 -0.05642778782397073 1101 6 0.09903824861371646 1101 7 -0.09723583437226324 1101 8 0.1737190946146825 1101 9 0.21731386009393105 1101 10-11 0.2262242723709491 1101 12-13 0.2634672095332533 1101 14-15 0.4010336690781102 1101 16-17 0.4432136563978162 1101 18-19 0.2960534093734637 1101 20-21 0.12280862576665896 1101 22-23 0.21805963825831753 1101 24-25 0.2640461954286053 1101 26-27 0.24539686419129936 1101 28-29 0.22351624148918603 1101 30-31 0.4391507394104437 1101 32-33 0.4481312161353159 1101 34-35 0.20930955219756697 1101 36-37 0.33036070382730287 1101 38-39 0.3559639404909447 1101 40-41 0.5898868681898009 1101 42-43 0.5846792406035277 1101 44-45 0.43136580786001133 1101 46-47 0.32091455730746077 1101 48-49 0.5224972331560664 1101 50-51 0.5805574860679314 1101 52-53 0.671502629558816 1101 54-55 0.5244665115567528 1101 56-57 0.4756837246155783 1101 58-59 0.6641778770553977 1101 60-61 0.6231753375787363 1101 62-63 0.530081680434467 1101 64-65 0.5832163270306516 1101 66-67 0.7572247011786608 1101 68-69 0.3577364681879196 1101 70-71 0.14800233277122743 1101 72-73 0.38120089569948945 1101 74-75 0.4034971135290917 1101 76-77 0.627730503830044 1101 78-79 0.6958105303941764 1101 80-81 0.5204470671911778 1101 82-83 1.0011606438144867 1101 84-85 0.8448515241809638 1101 86-87 0.5285402214362911 1101 88-89 0.7552873443202195 1101 90-91 1.054226275662085 1101 92-93 0.812321416774946 1101 94-95 0.6737700881966937 1101 96-97 0.5148231398030845 1101 98-99 0.4836541473735423 1101 100-101 0.637791876528297 1102 1 -0.03753110711321739 1102 2 0.10062042294471141 1102 3 0.12894494518045718 1102 4 0.19234576010249071 1102 5 -0.07176180324172776 1102 6 0.16110543295396695 1102 7 0.11994543122426649 1102 8 0.2731754377949045 1102 9 0.2473681320722676 1102 10-11 0.40788881216015227 1102 12-13 0.4031432057640316 1102 14-15 0.22623678636403355 1102 16-17 0.22793416478691597 1102 18-19 0.3001437490550245 1102 20-21 0.32234186449836244 1102 22-23 0.1291099916674625 1102 24-25 0.1689742456725014 1102 26-27 0.21994390956904653 1102 28-29 0.19401159993782358 1102 30-31 0.22179489221530702 1102 32-33 0.019280918831235283 1102 34-35 0.3590178703284579 1102 36-37 0.2026409995325693 1102 38-39 0.30491243921218825 1102 40-41 0.5437168581576586 1102 42-43 0.4934820535868667 1102 44-45 0.37566279921174583 1102 46-47 0.28498699959334317 1102 48-49 0.2646923346810368 1102 50-51 0.3907628668824614 1102 52-53 0.371795687700164 1102 54-55 0.6601422992969219 1102 56-57 0.38449467810283977 1102 58-59 0.2940821060764769 1102 60-61 0.35762302195675844 1102 62-63 0.38194647076378274 1102 64-65 0.558498828871052 1102 66-67 0.507606689933052 1102 68-69 0.45000105682014535 1102 70-71 0.14008560485556387 1102 72-73 0.023477753652382916 1102 74-75 0.21978661327462845 1102 76-77 0.4287413904363433 1102 78-79 0.0429893187006698 1102 80-81 0.24128324335636364 1102 82-83 0.6228546182603303 1102 84-85 0.567395566356975 1102 86-87 0.4008312930604241 1102 88-89 0.4843175526175578 1102 90-91 0.2021536975059277 1102 92-93 0.24070723683701445 1102 94-95 0.07731542406861891 1102 96-97 0.19871829479769332 1102 98-99 0.1700609699357436 1102 100-101 0.34838519042202876 1103 1 0.11463788473764325 1103 2 0.26967463635124034 1103 3 0.2420519075978511 1103 4 0.2271563678888171 1103 5 0.1224252264136112 1103 6 0.16287584293149848 1103 7 0.17367366424008424 1103 8 0.1730458017992902 1103 9 0.2662050528298252 1103 10-11 0.2969406943671302 1103 12-13 0.3621742077597432 1103 14-15 0.38677395627593825 1103 16-17 0.35637752107527376 1103 18-19 0.3718139943694041 1103 20-21 0.300006073544246 1103 22-23 0.38095805899060764 1103 24-25 0.3786527472200305 1103 26-27 0.36449863566196683 1103 28-29 0.30008407894371913 1103 30-31 0.5506546693786731 1103 32-33 0.38468792911513816 1103 34-35 0.3760418396749614 1103 36-37 0.3228449854104767 1103 38-39 0.2924099911568163 1103 40-41 0.5442503123504778 1103 42-43 0.3748468940091598 1103 44-45 0.19977142601096176 1103 46-47 0.38752191285674087 1103 48-49 0.24263078509600433 1103 50-51 -0.07071731466709963 1103 52-53 -0.054461370243160445 1103 54-55 0.23105703478564976 1103 56-57 0.22097635363181212 1103 58-59 0.13747768164905239 1103 60-61 -0.03671044726460693 1103 62-63 0.3231958635572987 1103 64-65 0.39387572881347666 1103 66-67 0.36951287749284845 1103 68-69 0.3851527652521618 1103 70-71 0.23072648948071262 1103 72-73 0.2800436008688756 1103 74-75 0.21665020319177586 1103 76-77 0.5194369458328012 1103 78-79 0.5087058933520083 1103 80-81 0.6115882577429446 1103 82-83 0.8785543715910151 1103 84-85 0.6627100919194042 1103 86-87 0.47008809599181944 1103 88-89 0.8755405862891905 1103 90-91 0.5998989423617545 1103 92-93 0.5415683014852029 1103 94-95 0.946283607928077 1103 96-97 1.3482213173695037 1103 98-99 1.3616003130699745 1103 100-101 1.5974033353196901 1104 1 0.07069028840170333 1104 2 0.16117297295346944 1104 3 0.07176593345470295 1104 4 0.11228662918339438 1104 5 -0.04475396941378307 1104 6 0.08061050987801366 1104 7 0.20435605538342827 1104 8 0.13752327295218691 1104 9 0.1891528252760395 1104 10-11 0.27773007853601683 1104 12-13 0.32711372714755527 1104 14-15 0.40246037624346087 1104 16-17 0.3117689683881508 1104 18-19 0.34688618752136335 1104 20-21 0.32920907803384836 1104 22-23 0.21683763333447814 1104 24-25 0.19308848264931555 1104 26-27 0.1994679852360477 1104 28-29 0.26728932944250516 1104 30-31 0.34853223896032404 1104 32-33 0.27972222237011835 1104 34-35 0.3318363503416961 1104 36-37 0.3023750001506684 1104 38-39 0.4591726138007317 1104 40-41 0.5983598336441318 1104 42-43 0.4798938406670459 1104 44-45 0.4077211766822231 1104 46-47 0.2883513198958383 1104 48-49 0.45078625911008885 1104 50-51 0.6658883527064248 1104 52-53 0.687903344494373 1104 54-55 0.9082434559594077 1104 56-57 0.7326964926865642 1104 58-59 0.3227522086240846 1104 60-61 0.6569156875007707 1104 62-63 0.5723954664648048 1104 64-65 0.7474426253884978 1104 66-67 0.4962756086978999 1104 68-69 0.6326399972569483 1104 70-71 0.7911602394475423 1104 72-73 0.6089115736465693 1104 74-75 0.5380079772543525 1104 76-77 0.8715977345173549 1104 78-79 1.05638352951782 1104 80-81 0.8618187692106183 1104 82-83 1.0498816279135 1104 84-85 0.8673296092807412 1104 86-87 0.8386588042687642 1104 88-89 1.1087608351113012 1104 90-91 1.3050785748461422 1104 92-93 1.3801334369574327 1104 94-95 1.6338857458586133 1104 96-97 1.5208642139734714 1104 98-99 1.2208906730828097 1104 100-101 1.5049684780293262 1105 1 0.1254658948498566 1105 2 0.21444295174737604 1105 3 0.1808955351037156 1105 4 0.15525623310467296 1105 5 0.14805982220654101 1105 6 0.10123674649867098 1105 7 0.16737054156548226 1105 8 0.27616439390807557 1105 9 0.27836766720923123 1105 10-11 0.18559198075467265 1105 12-13 0.2026271418664507 1105 14-15 0.2871433600896083 1105 16-17 0.022539278131034735 1105 18-19 0.22169161234572954 1105 20-21 0.3353853341056876 1105 22-23 0.2469815061121139 1105 24-25 0.27137199104323884 1105 26-27 0.3945403451381182 1105 28-29 0.2901538462848805 1105 30-31 0.4067050176586733 1105 32-33 0.3199403849515292 1105 34-35 0.16906714680528268 1105 36-37 0.1878167574415599 1105 38-39 0.270608980554087 1105 40-41 0.41182151938918565 1105 42-43 0.5872547672836106 1105 44-45 0.4726954007019728 1105 46-47 0.4599768956329484 1105 48-49 0.39985701524101813 1105 50-51 0.4737518652898345 1105 52-53 0.5221958592653522 1105 54-55 0.7643553988809799 1105 56-57 0.46769981232038305 1105 58-59 0.42213022742401307 1105 60-61 0.4565623455754455 1105 62-63 0.6778469062323431 1105 64-65 0.7110731711931031 1105 66-67 0.45120289375477896 1105 68-69 0.4041601131220638 1105 70-71 0.41238792828753645 1105 72-73 0.47012691466603584 1105 74-75 0.4389304119432822 1105 76-77 0.5856434247501241 1105 78-79 0.8183670525698759 1105 80-81 0.7685702805442887 1105 82-83 1.0179375068100072 1105 84-85 0.7412805263771354 1105 86-87 0.7605425455696242 1105 88-89 1.1833504583772445 1105 90-91 1.171504333480108 1105 92-93 1.2374783233265418 1105 94-95 1.3166698180070107 1105 96-97 1.4587488312113202 1105 98-99 1.5673859487544313 1105 100-101 1.528968619031346 1106 1 -0.05096317718913923 1106 2 0.06565763749809506 1106 3 -0.0032692282893194147 1106 4 0.12412071510142653 1106 5 -0.0472597063299105 1106 6 0.020713404244730782 1106 7 -0.03074523511426719 1106 8 0.05512511863530989 1106 9 0.16866895032847395 1106 10-11 0.124243514023469 1106 12-13 0.19846060700543688 1106 14-15 0.197908472574305 1106 16-17 0.055645273073906765 1106 18-19 0.07188186941244368 1106 20-21 0.005610612813136129 1106 22-23 0.15974359725976228 1106 24-25 0.08717390718111062 1106 26-27 0.25571567196341505 1106 28-29 0.29205676843378825 1106 30-31 0.2729116650219652 1106 32-33 0.2505207023957752 1106 34-35 0.10507764626786553 1106 36-37 0.05225594371915321 1106 38-39 0.2665720018630111 1106 40-41 0.3936345887456767 1106 42-43 0.3130575340072923 1106 44-45 0.0832553884422893 1106 46-47 0.1796015974885563 1106 48-49 0.12525143047732712 1106 50-51 0.4805952147983632 1106 52-53 0.5353710820520519 1106 54-55 0.526095135086905 1106 56-57 0.43839516270901413 1106 58-59 0.4313538815828508 1106 60-61 0.15182402021723007 1106 62-63 0.26845179927996554 1106 64-65 0.18714640311692676 1106 66-67 0.2979214250672335 1106 68-69 0.2006088821978551 1106 70-71 0.19652148010191794 1106 72-73 0.28546424223788236 1106 74-75 0.31746303190926994 1106 76-77 0.27364636876119164 1106 78-79 0.31016002420037836 1106 80-81 0.39097464060499476 1106 82-83 0.6005632722493743 1106 84-85 0.6068649807614825 1106 86-87 0.5760721336541117 1106 88-89 0.9114214070756752 1106 90-91 0.8169502899990029 1106 92-93 1.0853139339100792 1106 94-95 1.2939549589757924 1106 96-97 1.1911735139463282 1106 98-99 1.3117368990556173 1106 100-101 1.6178648187404612 1107 1 0.30480350836228354 1107 2 0.3886577694543405 1107 3 0.3388827331573303 1107 4 0.29539850196601236 1107 5 0.21745985503804377 1107 6 0.18326185075622448 1107 7 0.21330555302800747 1107 8 0.17860835268539432 1107 9 0.35780191876722967 1107 10-11 0.345129027360926 1107 12-13 0.3224642385677825 1107 14-15 0.46657810865162475 1107 16-17 0.45528140569705045 1107 18-19 0.37582001274854093 1107 20-21 0.29211828405401974 1107 22-23 0.2909656306344246 1107 24-25 0.3249343331074215 1107 26-27 0.42133032337373066 1107 28-29 0.23133042435668472 1107 30-31 0.39471391870824135 1107 32-33 0.39834101433402225 1107 34-35 0.40446922524683515 1107 36-37 0.44659936656938726 1107 38-39 0.5842011194707979 1107 40-41 0.6983639954719649 1107 42-43 0.7092016460326178 1107 44-45 0.6766480151696683 1107 46-47 0.5442045316581954 1107 48-49 0.6987637818191317 1107 50-51 0.4974907504893622 1107 52-53 0.8000736550408476 1107 54-55 0.8957141450041632 1107 56-57 0.6697438582780535 1107 58-59 0.7489062749294142 1107 60-61 0.8734380584655774 1107 62-63 0.9903642008757707 1107 64-65 1.1611178357745935 1107 66-67 1.0370649421042089 1107 68-69 0.8974614491419466 1107 70-71 0.7187678811335481 1107 72-73 1.1414287041289946 1107 74-75 1.1524380747950502 1107 76-77 0.8427285458970459 1107 78-79 1.3227935311312393 1107 80-81 1.3240330782588323 1107 82-83 1.651898361311936 1107 84-85 1.5675051186913187 1107 86-87 1.8946064553160333 1107 88-89 1.8672892327448771 1107 90-91 1.8519147447787034 1107 92-93 2.0040756628055725 1107 94-95 2.140087653230008 1107 96-97 1.6865028949134953 1107 98-99 1.6163098546622194 1107 100-101 1.676932780948821 1108 1 0.015595805392763396 1108 2 0.08284621826901883 1108 3 0.04570030576085671 1108 4 0.11585497907677222 1108 5 -0.03484459597337519 1108 6 0.016716516864988762 1108 7 0.05976756121346938 1108 8 -0.013547458865794226 1108 9 0.10300995517032874 1108 10-11 0.028197956581472283 1108 12-13 -0.017347731043990677 1108 14-15 -0.012187892891326157 1108 16-17 0.10370813857098682 1108 18-19 0.024300438235947297 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66-67 -2.0430635006713906 2316 68-69 -1.9345648568155553 2316 70-71 -2.101416127856293 2316 72-73 -2.2389702128202877 2316 74-75 -1.9957784545669028 2316 76-77 -1.9206825734044237 2316 78-79 -2.355112984339158 2316 80-81 -2.372152731007109 2316 82-83 -2.4784791340827894 2316 84-85 -1.9952695568478624 2316 86-87 -2.047081043872545 2316 88-89 -2.7607546836057573 2316 90-91 -2.518865822048614 2316 92-93 -2.590117725507344 2316 94-95 -2.8128149313490702 2316 96-97 -2.5638777163437076 2316 98-99 -2.5856665542197206 2316 100-101 -2.5976339915513833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 40.0 5 116.0 6 259.0 7 639.0 8 1601.0 9 2690.0 10 4195.0 11 6414.0 12 9254.0 13 12625.0 14 16008.0 15 21148.0 16 25615.0 17 31467.0 18 29630.0 19 25056.0 20 25922.0 21 27907.0 22 29904.0 23 32971.0 24 38003.0 25 44504.0 26 52737.0 27 62614.0 28 74450.0 29 87686.0 30 103106.0 31 121154.0 32 143395.0 33 170336.0 34 203845.0 35 240953.0 36 254911.0 37 186432.0 38 45244.0 39 764.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.213921341225024 21.8272522119206 16.452545314253516 21.506281132600854 2 17.64491659995538 22.921045083081328 36.80949131315934 22.62454700380395 3 20.868434249933212 25.314232685448605 30.491842464180426 23.325490600437757 4 15.683091099123084 19.22699087462095 35.35163408495461 29.738283941301365 5 14.201160690264805 37.74924328281678 33.8679332832761 14.18166274364231 6 31.495306077493073 37.52841428377257 16.645216323508045 14.331063315226306 7 27.687312905035554 33.24212636898326 20.90097107764356 18.169589648337627 8 26.389405748941936 35.47494621791236 19.37383120626544 18.761816826880263 9 26.071177989969108 16.700295133738187 20.046081545400305 37.1824453308924 10-11 24.97726857298194 27.09262704992946 27.115475649251735 20.81462872783686 12-13 25.977061328371253 24.35258247539858 27.712858826865528 21.95749736936464 14-15 22.98879100843428 25.659825971207677 26.0139532376714 25.337429782686645 16-17 22.205651454576987 28.734023556320036 26.67139261627008 22.388932372832897 18-19 22.285050217870896 27.240072309609616 29.196036001074237 21.27884147144525 20-21 23.02296386163893 26.28666265308987 29.35592403700521 21.33444944826599 22-23 22.753751909935414 26.198103656764687 29.305135031262008 21.743009402037888 24-25 22.660663030864324 26.447345233118174 29.27381753621585 21.618174199801647 26-27 22.523061304884475 26.57565956984102 29.157274314640492 21.744004810634014 28-29 22.469562714996684 26.694822410015707 29.18881322416929 21.646801650818322 30-31 22.654187035118884 26.434610823908773 29.14991493290714 21.761287208065205 32-33 22.581866022249635 26.425988279009076 29.115306091840676 21.87683960690061 34-35 22.71996756670619 26.5504473638575 29.049006144515634 21.680578924920674 36-37 22.877602180194103 26.440384116491995 28.988880850723774 21.69313285259013 38-39 22.678682918323613 26.57498860996586 28.977371798837936 21.768956672872594 40-41 22.77794585615964 26.444832886473975 28.839547533516797 21.93767372384959 42-43 22.755470774883882 26.525911483354502 28.686615642033924 22.03200209972769 44-45 22.803166863048922 26.641971246638303 28.5244052992275 22.030456591085276 46-47 23.12002632162343 26.83650269238899 28.12818855581182 21.915282430175754 48-49 23.1558648177489 26.961670803242487 27.828371512393208 22.054092866615406 50-51 23.018776131176306 27.15584767713894 27.667427591991235 22.15794859969352 52-53 23.164398675294315 27.25325106884776 27.462169678937908 22.120180576920013 54-55 23.156692178983036 27.13567615449871 27.428114791362994 22.279516875155252 56-57 23.065171576858244 27.37032344061569 27.401631931661253 22.162873050864814 58-59 23.180658494751967 27.154583420529114 27.476901221569 22.187856863149918 60-61 23.05401823042629 27.191953790256356 27.670047844557683 22.08398013475967 62-63 22.956367112335744 27.135624928378377 27.7391095882867 22.16889837099918 64-65 22.92398798280848 27.099821429408372 27.65983474018213 22.316355847601013 66-67 22.911198136303764 27.25791669000349 27.483590600810505 22.347294572882245 68-69 22.88215012266777 27.14305365768837 27.619148498892837 22.35564772075102 70-71 22.962151607760482 27.10528122714402 27.41302336023382 22.51954380486168 72-73 23.025541987748902 27.229460755261815 27.266463864319746 22.47853339266954 74-75 22.975955874615927 27.389080157942853 27.040718162373484 22.594245805067736 76-77 23.002040908629677 27.25544541090833 27.17333100238399 22.569182678078008 78-79 22.97817543547189 27.361343828871792 27.07117765472053 22.58930308093579 80-81 22.960639619943855 27.305169551919672 27.109653050191007 22.624537777945466 82-83 23.035117752445096 27.41352380885425 26.9990614487435 22.55229698995715 84-85 22.97155991381593 27.455142148874213 26.95295637880682 22.62034155850304 86-87 22.945539983558323 27.347157529848587 26.991022693079593 22.716279793513504 88-89 22.914174753277138 27.31602648708642 26.863029441761405 22.906769317875035 90-91 22.989662938200258 27.352788850690267 26.90021723999747 22.757330971112 92-93 22.880095237648867 27.367700751308817 26.9063465206856 22.845857490356718 94-95 22.8249305168236 27.338337371872086 26.82964553034528 23.007086580959033 96-97 22.957190784052912 27.323531148675062 26.685948577197475 23.03332949007455 98-99 22.709573218572572 27.381845127338888 26.574382803133485 23.334198850955055 100-101 22.94553838250899 27.354638409880877 26.391372981323375 23.30845022628676 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 675.0 1 495.5 2 331.5 3 393.5 4 378.5 5 365.5 6 644.0 7 1387.0 8 2290.0 9 2696.0 10 2420.5 11 2077.5 12 2070.5 13 2220.0 14 2418.0 15 2664.5 16 2912.5 17 3124.0 18 3428.0 19 3752.0 20 4136.0 21 4792.5 22 5727.5 23 6921.0 24 8505.0 25 10635.0 26 13340.5 27 16519.5 28 19795.5 29 22770.5 30 25587.0 31 28857.0 32 32693.0 33 36319.5 34 39829.5 35 43109.0 36 47287.5 37 51475.0 38 54314.0 39 55844.0 40 57799.5 41 61276.5 42 63877.0 43 67061.0 44 70832.0 45 73601.0 46 75410.0 47 77296.5 48 79458.0 49 79340.5 50 78082.5 51 77255.5 52 77552.0 53 77751.0 54 75706.0 55 71203.5 56 64994.0 57 59198.0 58 53912.0 59 47657.0 60 40132.0 61 33233.5 62 27018.5 63 21035.0 64 15706.5 65 11333.5 66 8394.0 67 6448.5 68 4749.0 69 3436.5 70 2580.0 71 1846.5 72 1239.0 73 871.0 74 580.0 75 328.0 76 210.5 77 138.5 78 72.0 79 40.0 80 30.5 81 22.0 82 12.5 83 4.0 84 3.0 85 4.0 86 4.0 87 3.5 88 4.0 89 1.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018560092987940625 2 0.0017810190240953126 3 0.0 4 0.0 5 0.0024371839277093754 6 0.0 7 4.6868921686718754E-5 8 0.0 9 4.6868921686718754E-5 10-11 0.0 12-13 0.004452547560238282 14-15 0.0025777906927695314 16-17 0.0 18-19 3.280824518070313E-4 20-21 8.67075051204297E-4 22-23 0.0014060676506015626 24-25 6.561649036140626E-4 26-27 1.6404122590351564E-4 28-29 7.030338253007812E-5 30-31 0.0 32-33 9.373784337343751E-5 34-35 0.0 36-37 0.0018044534849386718 38-39 0.007780240999995314 40-41 7.264682861441407E-4 42-43 0.0 44-45 7.499027469875001E-4 46-47 1.4060676506015625E-4 48-49 7.030338253007812E-5 50-51 0.0011014196596378907 52-53 1.8747568674687501E-4 54-55 0.0 56-57 1.6404122590351564E-4 58-59 7.030338253007812E-5 60-61 0.0015935433373484376 62-63 7.030338253007812E-5 64-65 0.0 66-67 2.3434460843359377E-5 68-69 7.030338253007812E-5 70-71 2.577790692769531E-4 72-73 2.577790692769531E-4 74-75 0.002507487310239453 76-77 7.030338253007812E-5 78-79 2.3434460843359375E-4 80-81 0.0014295021114449221 82-83 1.1717230421679688E-4 84-85 0.001734150102408594 86-87 3.7495137349375003E-4 88-89 0.001968494710842188 90-91 0.001171723042167969 92-93 0.0 94-95 0.0 96-97 1.8747568674687501E-4 98-99 5.62427060240625E-4 100-101 0.0015935433373484376 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2133610.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.3738466520514 #Duplication Level Percentage of deduplicated Percentage of total 1 76.81425279261406 46.37571918793192 2 13.242925115465779 15.99052660291462 3 4.3891202353700915 7.949642160829491 4 1.9964361401058117 4.821301174934467 5 1.0172675056512277 3.0708176195151022 6 0.6206724740532912 2.2483430861785654 7 0.38627869503081275 1.632479148912137 8 0.27985294658949467 1.3516639106015107 9 0.2034997826364698 1.105745820355802 >10 0.9850724106670468 10.42169231090701 >50 0.04657703225582192 1.8982855566745955 >100 0.017034872813530548 1.7278324596321264 >500 5.438444020016477E-4 0.21464040821258515 >1k 3.107682297151827E-4 0.430828703984347 >5k 1.5538411485759135E-4 0.7604818484157014 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8811 0.4129620689816789 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7402 0.3469237583250922 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4806 0.22525203762637033 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.3434460843359377E-5 9.373784337343751E-5 12-13 0.0 0.0 0.0 4.6868921686718754E-5 1.6404122590351564E-4 14-15 0.0 0.0 0.0 4.6868921686718754E-5 1.8747568674687501E-4 16-17 0.0 0.0 0.0 4.6868921686718754E-5 1.8747568674687501E-4 18-19 0.0 0.0 0.0 4.6868921686718754E-5 1.8747568674687501E-4 20-21 0.0 0.0 0.0 4.6868921686718754E-5 1.8747568674687501E-4 22-23 0.0 0.0 0.0 1.4060676506015627E-4 1.8747568674687501E-4 24-25 0.0 0.0 0.0 2.3434460843359378E-4 1.8747568674687501E-4 26-27 0.0 0.0 0.0 4.921236777105469E-4 1.8747568674687501E-4 28-29 0.0 0.0 0.0 0.0012185919638546877 1.8747568674687501E-4 30-31 0.0 0.0 0.0 0.0027652663795164067 1.8747568674687501E-4 32-33 0.0 0.0 0.0 0.005108712463852344 2.3434460843359378E-4 34-35 0.0 0.0 0.0 0.008577012668669531 2.3434460843359378E-4 36-37 0.0 0.0 0.0 0.015091792783123439 2.3434460843359378E-4 38-39 0.0 0.0 0.0 0.023996887903600002 2.3434460843359378E-4 40-41 0.0 0.0 0.0 0.036979579210821095 2.3434460843359378E-4 42-43 0.0 0.0 0.0 0.05017318066563242 2.577790692769532E-4 44-45 0.0 0.0 0.0 0.0668585167861043 3.046479909636719E-4 46-47 0.0 0.0 0.0 0.08834791737946485 3.5151691265039066E-4 48-49 0.0 0.0 0.0 0.10866559493065743 3.7495137349375003E-4 50-51 0.0 0.0 0.0 0.13320147543365468 4.2182029518046877E-4 52-53 0.0 0.0 0.0 0.16066666354207187 4.921236777105469E-4 54-55 0.0 0.0 0.0 0.18843649964145276 5.624270602406251E-4 56-57 0.0 0.0 0.0 0.2275720492498629 5.624270602406251E-4 58-59 0.0 0.0 0.0 0.27008216121971684 5.858615210839845E-4 60-61 0.0 0.0 0.0 0.31402177530101566 6.327304427707032E-4 62-63 0.0 0.0 0.0 0.3637497012106242 6.561649036140626E-4 64-65 0.0 0.0 0.0 0.41825825713227816 6.79599364457422E-4 66-67 2.3434460843359377E-5 0.0 0.0 0.4833591893551305 7.264682861441407E-4 68-69 4.6868921686718754E-5 0.0 0.0 0.5555138942918341 7.499027469875001E-4 70-71 4.6868921686718754E-5 0.0 0.0 0.6368549078791345 8.202061295175782E-4 72-73 4.6868921686718754E-5 0.0 0.0 0.7230468548610103 0.0010311162771078125 74-75 4.6868921686718754E-5 0.0 0.0 0.8171830840687848 0.0010311162771078125 76-77 4.6868921686718754E-5 0.0 0.0 0.9200134982494458 0.0010311162771078125 78-79 4.6868921686718754E-5 2.3434460843359377E-5 0.0 1.0389199525686514 0.0010311162771078125 80-81 4.6868921686718754E-5 4.6868921686718754E-5 0.0 1.165864426957129 0.001054550737951172 82-83 4.6868921686718754E-5 4.6868921686718754E-5 0.0 1.3059790683395747 0.0011248541204812502 84-85 4.6868921686718754E-5 4.6868921686718754E-5 0.0 1.4490464517882837 0.0011248541204812502 86-87 4.6868921686718754E-5 4.6868921686718754E-5 0.0 1.6036904588936123 0.001171723042167969 88-89 4.6868921686718754E-5 4.6868921686718754E-5 0.0 1.7725826181917033 0.001171723042167969 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5835 0.0 45.919163 1 GTATCAA 9160 0.0 34.333515 1 TATCAAC 11640 0.0 26.7692 2 ATCAACG 11815 0.0 26.33188 3 TCAACGC 11895 0.0 26.194717 4 CAACGCA 12110 0.0 25.808104 5 AACGCAG 12455 0.0 24.98233 6 ACGCAGA 14035 0.0 21.932997 7 GAGTACT 8585 0.0 21.607319 12-13 CGCAGAG 14160 0.0 21.57265 8 GTACTTT 8855 0.0 20.732203 14-15 AGAGTAC 12540 0.0 20.097572 10-11 GCAGAGT 14890 0.0 19.940731 9 AGTACTT 8880 0.0 19.73938 12-13 TAAGGTG 875 0.0 19.541988 5 CAGAGTA 14625 0.0 18.174395 10-11 GTGGTAT 3250 0.0 18.12567 1 TGGTATC 3135 0.0 18.029924 2 ACTTTTT 10500 0.0 17.868673 16-17 GCGGTAT 215 2.393039E-5 17.676949 1 >>END_MODULE