##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936771_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58133 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.759396556172913 31.0 31.0 34.0 30.0 34.0 2 31.69691913371063 31.0 31.0 34.0 30.0 34.0 3 31.823680181652417 31.0 31.0 34.0 30.0 34.0 4 35.56006055080591 37.0 35.0 37.0 33.0 37.0 5 35.49909689849139 37.0 35.0 37.0 33.0 37.0 6 35.684034885521136 37.0 35.0 37.0 33.0 37.0 7 35.64290506252903 37.0 35.0 37.0 33.0 37.0 8 35.69409801661707 37.0 35.0 37.0 33.0 37.0 9 37.17183011370478 39.0 37.0 39.0 33.0 39.0 10-11 37.13346980200575 39.0 37.0 39.0 33.5 39.0 12-13 37.09017253539298 39.0 37.0 39.0 33.0 39.0 14-15 37.88382674212582 40.0 37.0 41.0 32.5 41.0 16-17 37.79510777011336 39.0 37.0 40.5 32.5 41.0 18-19 37.63872499268918 39.0 35.5 41.0 33.0 41.0 20-21 37.681532004197265 39.0 35.0 41.0 33.0 41.0 22-23 37.54751174031961 39.0 35.0 41.0 33.0 41.0 24-25 37.29554641941754 39.0 35.0 41.0 32.5 41.0 26-27 36.92264290506253 38.5 35.0 40.5 32.0 41.0 28-29 36.71640032339635 38.0 35.0 40.0 31.5 41.0 30-31 36.61748920578673 38.0 35.0 40.0 32.0 41.0 32-33 36.282937402164 38.0 35.0 40.0 31.0 41.0 34-35 35.96645622968022 38.0 35.0 40.0 31.0 41.0 36-37 35.64931278275678 37.5 35.0 40.0 30.5 41.0 38-39 35.37949185488449 37.0 35.0 40.0 30.0 41.0 40-41 35.35841088538352 37.0 35.0 40.0 30.0 41.0 42-43 35.027987545800144 37.0 35.0 40.0 28.0 41.0 44-45 34.62114461665491 37.0 34.0 40.0 25.5 41.0 46-47 34.19992087110592 37.0 33.5 40.0 23.5 41.0 48-49 33.66929282851393 36.5 33.0 40.0 23.0 41.0 50-51 33.277277966043386 36.0 33.0 40.0 20.5 41.0 52-53 33.155703301051034 36.0 33.0 40.0 20.0 41.0 54-55 32.97128997299296 36.0 33.0 40.0 19.0 41.0 56-57 32.4202862401734 35.5 32.5 39.5 11.5 41.0 58-59 32.04615278757332 35.0 32.0 39.0 7.0 40.0 60-61 31.31324720898629 35.0 31.0 39.0 2.0 40.0 62-63 30.96398775222335 35.0 31.0 38.0 2.0 40.0 64-65 30.4929128033991 35.0 30.5 37.5 2.0 39.5 66-67 29.83696007431235 35.0 29.0 37.0 2.0 39.0 68-69 29.245652211308553 34.5 29.0 36.0 2.0 39.0 70-71 28.414626804052777 34.0 27.0 35.5 2.0 37.5 72-73 27.97338000791289 33.5 26.5 35.0 2.0 37.0 74-75 27.685815285638107 33.0 26.0 35.0 2.0 37.0 76-77 26.31146680886932 31.5 25.5 34.0 2.0 35.0 78-79 26.999913990332516 33.0 26.0 35.0 2.0 35.0 80-81 26.626993274044004 33.0 26.0 35.0 2.0 35.0 82-83 26.189857740009977 33.0 24.5 35.0 2.0 35.0 84-85 25.75144926289715 33.0 23.5 35.0 2.0 35.0 86-87 25.597328539727865 33.0 21.5 35.0 2.0 35.0 88-89 25.061058262948755 32.5 19.0 35.0 2.0 35.0 90-91 24.365876524521354 32.0 10.5 34.0 2.0 35.0 92-93 23.835919357335765 31.0 2.0 34.0 2.0 35.0 94-95 23.125926754167168 31.0 2.0 34.0 2.0 35.0 96-97 22.30293464985464 31.0 2.0 34.0 2.0 35.0 98-99 21.709949598334852 31.0 2.0 34.0 2.0 35.0 100-101 19.133280580737274 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.47000546539573307 1101 2 0.6027255442906174 1101 3 0.3723780504334684 1101 4 0.4611621670121977 1101 5 0.5378265240138873 1101 6 0.6811986376006303 1101 7 0.3551912071966896 1101 8 0.6055681911860518 1101 9 0.6589538170295626 1101 10-11 0.7666448010706901 1101 12-13 0.46614230822002867 1101 14-15 0.7868344833399306 1101 16-17 0.36931869924064387 1101 18-19 0.7158835199395241 1101 20-21 0.7362403067994236 1101 22-23 0.7899144878363487 1101 24-25 1.135735839319075 1101 26-27 0.9902007855406438 1101 28-29 0.769134419199041 1101 30-31 1.0255193126562219 1101 32-33 0.9434664908543127 1101 34-35 1.0345383467746885 1101 36-37 1.1954980653212033 1101 38-39 0.8468681589912919 1101 40-41 1.2642835107944137 1101 42-43 1.0935535305494497 1101 44-45 1.5000333693505468 1101 46-47 2.390774266654695 1101 48-49 2.3596281177141165 1101 50-51 2.399411046967373 1101 52-53 2.3146176670496246 1101 54-55 2.5050840643665424 1101 56-57 3.1770859017289723 1101 58-59 2.5476393337127057 1101 60-61 2.1123176450960166 1101 62-63 2.175924339549546 1101 64-65 2.4454154293622743 1101 66-67 3.0041590115705077 1101 68-69 2.2645157248975174 1101 70-71 2.69090306302612 1101 72-73 2.9635994921230164 1101 74-75 3.6235379133528127 1101 76-77 3.100470544985523 1101 78-79 3.347719029562519 1101 80-81 3.8570640466104535 1101 82-83 4.299945676294609 1101 84-85 4.253233462076725 1101 86-87 4.443313733712571 1101 88-89 4.346538280464241 1101 90-91 4.1545182258272035 1101 92-93 4.616629417844631 1101 94-95 4.463193399919646 1101 96-97 5.192816297185178 1101 98-99 4.549439823436565 1101 100-101 3.9472169084422397 1102 1 0.20333879872906735 1102 2 0.4193922109572803 1102 3 0.5390447171001327 1102 4 0.944495500345532 1102 5 0.8211598573472187 1102 6 0.6478653042672917 1102 7 0.7218578738633568 1102 8 0.6222348578527175 1102 9 0.7256204836962254 1102 10-11 0.9499781344040272 1102 12-13 0.9828089748867015 1102 14-15 1.2785011500065906 1102 16-17 1.4776520325739781 1102 18-19 0.915883519939527 1102 20-21 0.7195736401327579 1102 22-23 0.27324782116968294 1102 24-25 0.7107358393190779 1102 26-27 0.6985341188739724 1102 28-29 0.4774677525323767 1102 30-31 1.4255193126562276 1102 32-33 1.8184664908543127 1102 34-35 1.6012050134413514 1102 36-37 2.087164731987869 1102 38-39 1.588534825657952 1102 40-41 2.6976168441277437 1102 42-43 2.185220197216111 1102 44-45 2.983366702683888 1102 46-47 3.4491075999880323 1102 48-49 3.0512947843807865 1102 50-51 3.516077713634033 1102 52-53 3.364617667049629 1102 54-55 3.530084064366534 1102 56-57 4.268752568395634 1102 58-59 4.08930600037937 1102 60-61 4.170650978429354 1102 62-63 4.325924339549545 1102 64-65 3.15374876269561 1102 66-67 3.737492344903842 1102 68-69 3.5228490582308503 1102 70-71 2.2075697296927856 1102 72-73 2.405266158789683 1102 74-75 2.2485379133528127 1102 76-77 2.1171372116521923 1102 78-79 2.222719029562519 1102 80-81 1.7237307132771207 1102 82-83 1.891612342961274 1102 84-85 2.078233462076728 1102 86-87 1.251647067045905 1102 88-89 0.9298716137975731 1102 90-91 1.1128515591605392 1102 92-93 1.3166294178446343 1102 94-95 1.7715267332529798 1102 96-97 3.4511496305185148 1102 98-99 2.557773156769901 1102 100-101 3.497216908442244 1103 1 -0.049835804445535814 1103 2 -0.6512427096776392 1103 3 -0.24032036226494569 1103 4 -0.47534576949573903 1103 5 -0.27090363471626944 1103 6 -0.36642041001842074 1103 7 -0.043221491216009156 1103 8 -0.8864953008774421 1103 9 -1.033109675033927 1103 10-11 -0.9750218655959699 1103 12-13 -0.1390164219386989 1103 14-15 -0.5544353579299184 1103 16-17 -0.13822098329903554 1103 18-19 -0.49086251180651175 1103 20-21 0.0025101480692697464 1103 22-23 0.061343059264920896 1103 24-25 -0.4011689225856827 1103 26-27 -0.41575159541174145 1103 28-29 -0.45189732683270734 1103 30-31 -0.05265529051838058 1103 32-33 0.6228315702193896 1103 34-35 0.13057009280642973 1103 36-37 -0.0033114584883193743 1103 38-39 -0.07098898386585262 1103 40-41 0.32182319333409737 1103 42-43 -0.8088274218315021 1103 44-45 -1.007109487792313 1103 46-47 -0.7862098603294285 1103 48-49 -1.0633877553017541 1103 50-51 -0.8859064133500922 1103 52-53 -0.46554106310910726 1103 54-55 -0.5921381578556861 1103 56-57 -0.34791409827103337 1103 58-59 -0.04085272977935617 1103 60-61 -0.47934902157064485 1103 62-63 -0.36653597791077175 1103 64-65 -0.48990203095518936 1103 66-67 -0.5867140043025074 1103 68-69 -0.6394525290707342 1103 70-71 -1.7642556671326126 1103 72-73 -0.6689401904166665 1103 74-75 -0.21058907077417288 1103 76-77 0.48578800530298594 1103 78-79 -0.10466192281843689 1103 80-81 -0.0750788105324034 1103 82-83 0.13407266042159094 1103 84-85 0.5853763192195842 1103 86-87 0.36990103529987195 1103 88-89 1.2739192328451914 1103 90-91 1.690629336938315 1103 92-93 0.5825024337176465 1103 94-95 1.5266854634117095 1103 96-97 1.7821020114708936 1103 98-99 2.285947759944502 1103 100-101 1.548804210029541 1104 1 -0.06570882031855163 1104 2 -0.01632207475700298 1104 3 -0.20857433051891405 1104 4 0.33417804002807117 1104 5 -0.46137982519246634 1104 6 -0.08070612430413604 1104 7 -0.4083008562953694 1104 8 0.06588565150350689 1104 9 -0.06485570677995867 1104 10-11 -0.030577421151527062 1104 12-13 -0.17869896162124377 1104 14-15 -0.30840361189817145 1104 16-17 -0.0588559039339529 1104 18-19 -0.403560924504923 1104 20-21 0.12949427505339628 1104 22-23 0.30737480529666783 1104 24-25 -0.3297403511571062 1104 26-27 -0.3522595319196782 1104 28-29 -0.12650050143588487 1104 30-31 0.4235351856720939 1104 32-33 0.22600617339399776 1104 34-35 -0.5916521294157917 1104 36-37 0.43319647801961736 1104 38-39 -0.5074969203737894 1104 40-41 -0.16230379079289037 1104 42-43 -0.0866051996092807 1104 44-45 0.6198746391918206 1104 46-47 -0.8973209714405428 1104 48-49 -1.4205306124446153 1104 50-51 -0.576382603826282 1104 52-53 -0.23538233295037259 1104 54-55 -0.5762651419826668 1104 56-57 -1.1018823522392864 1104 58-59 -1.6440273329539608 1104 60-61 -1.1142696564912846 1104 62-63 -1.5649486763234712 1104 64-65 -1.5613306023837588 1104 66-67 -1.6105235281120294 1104 68-69 -1.6632620528802597 1104 70-71 -1.7245731274500713 1104 72-73 -2.121321142797619 1104 74-75 -2.202652562837663 1104 76-77 -1.6253231058081248 1104 78-79 -2.0252968434533543 1104 80-81 -2.130634366087957 1104 82-83 -1.9929114665625356 1104 84-85 -1.8828776490343841 1104 86-87 -1.5666069012080648 1104 88-89 -1.1149696560436944 1104 90-91 -1.158577012268033 1104 92-93 -1.2349578837426698 1104 94-95 -1.520933584207338 1104 96-97 -1.5036122742433946 1104 98-99 -1.872782398785656 1104 100-101 -1.9829418217164871 1105 1 0.33475956375638916 1105 2 0.2366053257113805 1105 3 0.5986075586301851 1105 4 0.44476872438924886 1105 5 0.013509584123177376 1105 6 -0.01279043343762254 1105 7 0.4857923000928679 1105 8 -0.20317497821285713 1105 9 0.23491010118257094 1105 10-11 0.16978687757342215 1105 12-13 -0.14929485024990896 1105 14-15 -0.019176445621823746 1105 16-17 0.391313234759771 1105 18-19 -0.6913569172189469 1105 20-21 0.0820053341218312 1105 22-23 0.16204563537733208 1105 24-25 0.07931507429176321 1105 26-27 -0.25337844943203436 1105 28-29 -0.08291476112883345 1105 30-31 0.2527051050059512 1105 32-33 -0.06855536706918741 1105 34-35 -0.21928678983733363 1105 36-37 -1.4494472898700508 1105 38-39 -0.7150170869103576 1105 40-41 -1.024650915435089 1105 42-43 -0.9575393656254079 1105 44-45 -0.25652400769862993 1105 46-47 -1.0116847497387411 1105 48-49 -1.0510276199907977 1105 50-51 -0.16329387106542015 1105 52-53 -0.24357905426185056 1105 54-55 0.06177805343757825 1105 56-57 0.348807213204374 1105 58-59 0.653786874696312 1105 60-61 0.8430553500140512 1105 62-63 0.26239974938560806 1105 64-65 -0.7263058821131345 1105 66-67 -0.8795841578283969 1105 68-69 -0.8089815428620462 1105 70-71 -1.0477581391596722 1105 72-73 -1.0273841144343585 1105 74-75 -0.39859323418817283 1105 76-77 -0.45540377195436577 1105 78-79 -1.2888929922954055 1105 80-81 -0.3982638222420043 1105 82-83 -0.6997810996616742 1105 84-85 -0.24717637398884662 1105 86-87 -0.49179555590491475 1105 88-89 -0.09376226598384818 1105 90-91 1.2297914498709233 1105 92-93 -0.29129407942312824 1105 94-95 1.0205704490999743 1105 96-97 1.4885813245075852 1105 98-99 1.087144741469352 1105 100-101 0.5286376734695644 1106 1 0.5420393637008196 1106 2 0.4049854312962644 1106 3 0.206276355518213 1106 4 -0.13205817197085423 1106 5 0.4697474279686915 1106 6 0.3648144568096683 1106 7 0.3896544840328531 1106 8 0.13664163751373337 1106 9 0.639744777481539 1106 10-11 0.8328877389238016 1106 12-13 0.53464513307879 1106 14-15 0.6964390031139374 1106 16-17 0.21945994217849574 1106 18-19 0.49286092106946455 1106 20-21 0.27705951583897814 1106 22-23 0.5492365217346631 1106 24-25 0.30423866417783785 1106 26-27 0.7759352488174827 1106 28-29 0.03989713106344084 1106 30-31 0.4940221375149818 1106 32-33 0.9219975643006393 1106 34-35 -0.13523566452474967 1106 36-37 0.4216280088240296 1106 38-39 0.1650884979743381 1106 40-41 -0.004784285815759404 1106 42-43 -0.31972330560874695 1106 44-45 0.04494862358783536 1106 46-47 -0.9812596316503885 1106 48-49 -0.6734227297435069 1106 50-51 -1.1798262411682288 1106 52-53 -0.9811450448147809 1106 54-55 -0.9264131107747033 1106 56-57 -0.5174056236947635 1106 58-59 -0.4010894798466147 1106 60-61 -0.6262416769378802 1106 62-63 -0.515177355365708 1106 64-65 0.34329678529447705 1106 66-67 0.22294432230497208 1106 68-69 0.8828773068184184 1106 70-71 1.4630782042690562 1106 72-73 0.9119045768687819 1106 74-75 1.197266726912133 1106 76-77 0.7768829743640531 1106 78-79 1.6245551877546092 1106 80-81 1.4854538771189283 1106 82-83 2.384408953130766 1106 84-85 2.6350131230936746 1106 86-87 2.147409778910312 1106 88-89 3.140747320012263 1106 90-91 3.551269638256585 1106 92-93 3.9960079489180806 1106 94-95 3.895396789750155 1106 96-97 3.5734660146993065 1106 98-99 3.7392703319111398 1106 100-101 2.8715106937529775 1107 1 0.04258611055702488 1107 2 -0.4886723051717503 1107 3 -0.13407356246975866 1107 4 -0.24851525234264216 1107 5 -0.33260358351299857 1107 6 -0.5876185667004492 1107 7 -0.3878195454914817 1107 8 0.12707356753013244 1107 9 -0.06255155931452094 1107 10-11 -0.6497530483916734 1107 12-13 -0.938427584253084 1107 14-15 -0.3314450865525487 1107 16-17 -0.8132081824797837 1107 18-19 -0.5532025015658562 1107 20-21 -0.3737059297597156 1107 22-23 -0.4294403508733282 1107 24-25 -0.6123824402508191 1107 26-27 0.2286416457556939 1107 28-29 0.41510216113452714 1107 30-31 0.27901393631213267 1107 32-33 -0.08825393925321379 1107 34-35 0.07539856182845739 1107 36-37 -0.21874924650676064 1107 38-39 -0.8716802281054825 1107 40-41 -0.48168423114107384 1107 42-43 -0.6844034586978651 1107 44-45 -0.37577308226235573 1107 46-47 0.2520645892353457 1107 48-49 0.42253134352056776 1107 50-51 0.9292497566447935 1107 52-53 1.4984886347915634 1107 54-55 0.7618044944740632 1107 56-57 1.0956342888257424 1107 58-59 0.7887683659707712 1107 60-61 0.7679628063863397 1107 62-63 0.805763049226961 1107 64-65 0.7123509132332408 1107 66-67 0.46679342017266023 1107 68-69 0.6844081980157988 1107 70-71 0.15676327807988244 1107 72-73 -0.010594056264078944 1107 74-75 -0.3313007963246086 1107 76-77 -0.4772176270574846 1107 78-79 -1.2598078521579126 1107 80-81 -1.0969682114540618 1107 82-83 -1.1511295925225973 1107 84-85 -1.6911213766329496 1107 86-87 -1.509643255534744 1107 88-89 -0.4795369883529652 1107 90-91 -0.3022022042803201 1107 92-93 -1.5408974638757975 1107 94-95 -1.9561614387900335 1107 96-97 -0.9026138103417054 1107 98-99 -0.2150763055956908 1107 100-101 0.4783997041411645 1108 1 0.22000546539573307 1108 2 -0.3238281280257702 1108 3 0.1384797453487181 1108 4 0.20692487887660604 1108 5 0.2494084449178402 1108 6 0.008882253419834285 1108 7 -0.3222099227468078 1108 8 -0.04979904045237049 1108 9 0.31771087917645247 1108 10-11 0.12102333214414074 1108 12-13 0.6448146246042157 1108 14-15 0.6455915454868233 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2316 68-69 0.4653929178799707 2316 70-71 0.6939732384647179 2316 72-73 0.9346521237019623 2316 74-75 0.6595028256335134 2316 76-77 0.5868740537574553 2316 78-79 1.6108769242993617 2316 80-81 1.624169309768348 2316 82-83 1.579770237698117 2316 84-85 1.544022935760939 2316 86-87 2.00691022494064 2316 88-89 1.2864505611659958 2316 90-91 1.0619743661780845 2316 92-93 0.2859276634586685 2316 94-95 -0.42277151236105226 2316 96-97 0.2344829638518462 2316 98-99 0.30558017431375717 2316 100-101 0.9489712944071549 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 0.0 4 5.0 5 7.0 6 50.0 7 54.0 8 45.0 9 98.0 10 129.0 11 210.0 12 263.0 13 350.0 14 497.0 15 598.0 16 851.0 17 963.0 18 941.0 19 718.0 20 681.0 21 733.0 22 780.0 23 838.0 24 960.0 25 990.0 26 1096.0 27 1247.0 28 1491.0 29 1666.0 30 2117.0 31 2421.0 32 2999.0 33 3605.0 34 5006.0 35 7085.0 36 7631.0 37 8071.0 38 2891.0 39 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.05574673090158 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29.969466305422955 21.177482475379524 44-45 18.13857877625445 28.29460031307519 32.04634200884179 21.520478901828564 46-47 22.000412846403936 33.13522439922247 32.52627595341717 12.338086800956427 48-49 19.25756455025545 30.633203172036538 33.720090138131525 16.38914213957649 50-51 11.700755164880533 33.57387370340426 39.19632566700497 15.529045464710233 52-53 16.92842275471763 36.45777785423082 29.095350317375672 17.518449073675882 54-55 13.826529734565035 30.2059142282087 33.08561696857099 22.88193906865528 56-57 20.10734006502331 29.16329795469011 30.951438941737052 19.777923038549535 58-59 24.683054375311787 28.904408855555364 30.21261589802694 16.199920871105913 60-61 17.416097569366798 32.41188309565995 36.78289439733026 13.38912493764299 62-63 15.017287943164812 32.27684791770595 35.20719728897528 17.498666850153956 64-65 14.133968658077167 37.97584848536976 31.44857481980975 16.44160803674333 66-67 17.692188601998865 30.437961226841896 29.023102196686906 22.846747974472333 68-69 14.506390518294257 31.69972304887069 32.88063578346206 20.913250649372987 70-71 21.520478901828564 31.74272788261401 28.52166583523988 18.215127380317547 72-73 18.19620525347049 26.495278069254987 37.36345965286498 17.945057024409543 74-75 13.968830096502845 37.5285982144393 30.750176319818344 17.7523953692395 76-77 17.078226465402313 29.078398486216834 30.40381886208231 23.439556186298542 78-79 17.058297352622436 30.206595221302873 33.82072144909088 18.914385976983812 80-81 20.727532342416737 29.383429672447015 30.730456922653453 19.158581062482796 82-83 16.458232986995114 28.127365306543727 36.57452005779949 18.839881648661667 84-85 15.627634136691265 31.17957716192737 33.22323717121674 19.96955153016463 86-87 16.34497944298223 32.23323183843389 30.63339698267706 20.78839173590683 88-89 15.927095070573968 31.68818434384703 27.520836738029093 24.863883847549907 90-91 18.231469217140006 26.429050625290284 30.294325082139235 25.04515507543048 92-93 20.643180293464987 31.643816765004384 30.746735933118885 16.966267008411744 94-95 18.1755629332737 29.890079644952095 30.915314881392668 21.019042540381538 96-97 11.071450074400282 31.480350584450772 35.416254526375546 22.031944814773404 98-99 16.875822502816938 33.47296169824791 26.583291043428147 23.067924755507004 100-101 14.879672475186211 29.334457192988495 29.19254124163556 26.593329090189737 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3997.0 1 2409.5 2 707.5 3 516.5 4 401.5 5 373.5 6 516.5 7 924.5 8 1182.0 9 1003.0 10 665.5 11 415.5 12 323.0 13 288.0 14 247.5 15 215.0 16 219.0 17 217.0 18 201.0 19 186.5 20 184.0 21 192.0 22 185.5 23 199.5 24 225.0 25 241.0 26 277.0 27 289.0 28 323.0 29 360.5 30 365.5 31 394.0 32 402.5 33 385.5 34 379.0 35 375.5 36 369.5 37 346.0 38 299.5 39 267.5 40 254.0 41 258.5 42 260.5 43 273.5 44 344.0 45 504.0 46 1068.5 47 4741.0 48 12094.0 49 11402.0 50 4287.5 51 1346.0 52 490.5 53 271.5 54 219.0 55 182.0 56 171.5 57 143.0 58 127.5 59 123.5 60 112.5 61 101.5 62 72.0 63 44.5 64 29.0 65 26.5 66 29.0 67 26.5 68 17.5 69 12.5 70 11.5 71 6.0 72 2.0 73 2.0 74 1.5 75 0.5 76 0.5 77 0.5 78 0.5 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02236251354652263 2 0.005160580049197529 3 0.0 4 0.0 5 0.0 6 0.0017201933497325102 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.600966748662551E-4 22-23 0.0 24-25 0.005160580049197529 26-27 0.0034403866994650203 28-29 0.006020676724063785 30-31 0.014621643472726334 32-33 8.600966748662551E-4 34-35 0.005160580049197529 36-37 0.004300483374331275 38-39 8.600966748662551E-4 40-41 0.0034403866994650203 42-43 8.600966748662551E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0034403866994650203 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 8.600966748662551E-4 78-79 0.0 80-81 0.00860096674866255 82-83 0.0017201933497325102 84-85 0.0034403866994650203 86-87 0.0034403866994650203 88-89 0.004300483374331275 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0025802900245987647 98-99 0.004300483374331275 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 58133.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.80630622882012 #Duplication Level Percentage of deduplicated Percentage of total 1 82.61247177349314 16.362479142655634 2 8.8153552197325 3.491992499956995 3 2.86607608129234 1.7029914162351847 4 1.5112037519541428 1.197254571413827 5 0.7208615598401946 0.7138802401389917 6 0.5297898210873719 0.6295907660020986 7 0.28660760812923397 0.3973646637882098 8 0.26055237102657636 0.4128464039358024 9 0.21712697585548027 0.38704350368981477 >10 1.4504081987146085 5.5063389124937645 >50 0.2258120548896995 3.337175098481069 >100 0.2952926871634532 12.105000602067673 >500 0.11290602744484975 16.362479142655634 >1k 0.09553586937641133 37.3935630364853 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4077 7.0132282868594435 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3454 5.941547829976089 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3439 5.915744929730102 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2203 3.789585949460719 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1612 2.772951679768806 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1287 2.21388884110574 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1228 2.1123974334715223 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1173 2.017786799236234 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1150 1.9782223521923863 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1078 1.8543684310116457 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1037 1.7838405036726128 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 982 1.6892298694373247 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 918 1.5791374950544441 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 881 1.5154903411143412 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 878 1.5103297610651438 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 813 1.3985171933325304 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 730 1.2557411453047322 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 716 1.2316584384084772 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 644 1.1078045172277364 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 636 1.0940429704298764 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 627 1.0785612302822838 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 580 0.9977121428448558 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 579 0.9959919494951233 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 528 0.9082620886587652 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 465 0.7998899076256172 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 444 0.7637658472812344 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 376 0.6467926994994238 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 363 0.6244301859529011 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 352 0.6055080591058435 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 332 0.5711041921111933 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 265 0.4558512376791151 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 235 0.40424543718713984 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 235 0.40424543718713984 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 224 0.3853233103400822 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 215 0.36984157019248964 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 206 0.3543598300448971 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 202 0.347479056645967 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 200 0.344038669946502 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.34059828324703695 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 185 0.31823576970051437 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.3165155763507818 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 181 0.3113549963015843 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 177 0.30447422290265425 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 163 0.2803915160063991 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 158 0.27179054925773655 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 158 0.27179054925773655 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 158 0.27179054925773655 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 140 0.2408270689625514 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 139 0.23910687561281888 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 139 0.23910687561281888 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 133 0.2287857155144238 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 129 0.2219049421154938 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 125 0.21502416871656377 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 120 0.2064232019679012 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 117 0.20126262191870364 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112 0.1926616551700411 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 105 0.18062030172191354 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 102 0.175459721672716 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.16685875492405347 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 96 0.16513856157432094 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 91 0.1565375948256584 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 91 0.1565375948256584 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 90 0.1548174014759259 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 87 0.14965682142672837 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 84 0.14449624137753084 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 84 0.14449624137753084 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 83 0.14277604802779834 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 79 0.13589527462886827 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 77 0.13245488792940327 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 77 0.13245488792940327 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 77 0.13245488792940327 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 74 0.12729430788020574 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 74 0.12729430788020574 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 74 0.12729430788020574 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 72 0.12385392118074072 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 67 0.11525295443207816 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 63 0.10837218103314813 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 61 0.1049317943336831 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 61 0.1049317943336831 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 59 0.10149140763421809 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 8.60096674866255E-4 0.0 16-17 0.0 0.0 0.0 0.00172019334973251 0.0 18-19 0.0 0.0 0.0 0.00172019334973251 0.0 20-21 0.0 0.0 0.0 0.00344038669946502 0.0 22-23 0.0 0.0 0.0 0.00688077339893004 0.0 24-25 0.0 0.0 0.0 0.009461063423528805 0.0 26-27 0.0 0.0 0.0 0.023222610221388884 0.0 28-29 0.0 0.0 0.0 0.04816541379251028 0.0 30-31 0.0 0.0 0.0 0.1032116009839506 0.0 32-33 0.0 0.0 0.0 0.15997798152512344 0.0 34-35 0.0 0.0 0.0 0.2004025252438374 0.0 36-37 0.0 0.0 0.0 0.25114822906094647 0.0 38-39 0.0 0.0 0.0 0.3319973164983744 0.0 40-41 0.0 0.0 0.0 0.4085459205614711 0.0 42-43 0.0 0.0 0.0 0.4704728811518415 0.0 44-45 0.0 0.0 0.0 0.5048767481464916 0.0 46-47 0.0 0.0 0.0 0.5444411951903394 0.0 48-49 0.0 0.0 0.0 0.5874460289336522 0.0 50-51 0.0 0.0 0.0 0.6381917327507611 0.0 52-53 0.0 0.0 0.0 0.7224812068876543 0.0 54-55 0.0 0.0 0.0 0.7947293275764196 0.0 56-57 0.0 0.0 0.0 0.9461063423528805 0.0 58-59 0.0 0.0 0.0 1.1215660640255964 0.0 60-61 0.0 0.0 0.0 1.275523368826656 0.0 62-63 0.0 0.0 0.0 1.3838955498598042 0.0 64-65 0.0 0.0 0.0 1.5507543047838577 0.0 66-67 0.0 0.0 0.0 1.8371664975143207 0.0 68-69 0.0 0.0 0.0 2.240551838026594 0.0 70-71 0.0 0.0 0.0 2.5828703146233636 0.0 72-73 0.0 0.0 0.0 2.9226085011955343 0.0 74-75 0.0 0.0 0.0 3.4593088263120775 0.0 76-77 0.0 0.0 0.0 3.6932551218756986 0.0 78-79 0.0 0.0 0.0 4.093200075688507 0.0 80-81 0.0 0.0 0.0 4.530129186520565 0.0 82-83 0.0 0.0 0.0 4.822562055975092 0.0 84-85 0.0 0.0 0.0 5.297335420501264 0.0 86-87 0.0 0.0 0.0 5.95530937677395 0.0 88-89 0.0 0.0 0.0 6.768100734522561 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 185 0.0 51.339256 9 TTGCGTT 80 2.2025597E-7 41.5885 4 CTTTGCG 85 3.5443372E-7 39.14212 2 GCTTTGC 85 3.5443372E-7 39.14212 1 TTTGCGT 85 3.5443372E-7 39.14212 3 CAGAAAA 195 0.0 28.006224 10-11 ATCTGTC 35 0.008335609 27.159838 82-83 ACAACAC 35 0.008335609 27.159838 94-95 ACGCCTG 35 0.0083531495 27.148146 88-89 AACAACA 50 0.0016387613 23.76486 94-95 GAGTACT 540 0.0 21.10614 12-13 AGTACTT 535 0.0 19.971931 12-13 GTACTTT 560 0.0 19.92834 14-15 AGAAAAA 275 0.0 19.85896 12-13 TACTTTT 565 0.0 19.33173 14-15 ACTTTTT 595 0.0 18.756086 16-17 GCAGAAA 260 0.0 17.35168 10-11 GAAAAAA 455 0.0 15.133798 12-13 GCAGAGA 190 6.639311E-8 15.009384 94-95 CTTTTTT 825 0.0 13.527116 16-17 >>END_MODULE