##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936769_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 54846 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.822539474164024 31.0 31.0 34.0 30.0 34.0 2 31.89085803887248 31.0 31.0 34.0 30.0 34.0 3 31.947726361083763 33.0 31.0 34.0 30.0 34.0 4 35.596780075119426 37.0 35.0 37.0 33.0 37.0 5 35.52169711555993 37.0 35.0 37.0 33.0 37.0 6 35.68637639937279 37.0 35.0 37.0 33.0 37.0 7 35.665773256025965 37.0 35.0 37.0 33.0 37.0 8 35.68451664661051 37.0 35.0 37.0 33.0 37.0 9 37.18593881048755 39.0 37.0 39.0 33.0 39.0 10-11 37.11114757685155 39.0 37.0 39.0 33.5 39.0 12-13 37.074481274842285 39.0 37.0 39.0 33.0 39.0 14-15 37.88656419793604 40.0 37.0 41.0 32.5 41.0 16-17 37.786511322612405 39.0 37.0 40.5 32.5 41.0 18-19 37.74606170003282 39.0 35.5 41.0 33.0 41.0 20-21 37.76650986398279 39.0 36.0 41.0 33.0 41.0 22-23 37.608841118768915 39.0 35.0 41.0 33.0 41.0 24-25 37.36701856106188 39.0 35.0 41.0 32.5 41.0 26-27 36.95540239944572 38.5 35.0 40.5 32.5 41.0 28-29 36.784049885132916 38.0 35.0 40.0 32.0 41.0 30-31 36.74979032199249 38.0 35.0 40.0 32.0 41.0 32-33 36.372807497356234 38.0 35.0 40.0 31.0 41.0 34-35 36.01176931772599 38.0 35.0 40.0 31.0 41.0 36-37 35.710434671626004 37.5 35.0 40.0 30.5 41.0 38-39 35.42812602559896 37.0 35.0 40.0 30.0 41.0 40-41 35.41444225650002 37.5 35.0 40.0 30.0 41.0 42-43 35.04198118367793 37.0 35.0 40.0 28.5 41.0 44-45 34.71746344309521 37.0 35.0 40.0 26.5 41.0 46-47 34.246736316230894 37.0 33.5 40.0 23.5 41.0 48-49 33.668836378222665 36.5 33.0 40.0 23.0 41.0 50-51 33.23041789738541 36.0 33.0 40.0 20.5 41.0 52-53 33.09836633482843 36.0 33.0 40.0 20.0 41.0 54-55 32.83602268169055 36.0 33.0 40.0 17.0 41.0 56-57 32.26847901396638 35.5 32.0 39.5 10.5 41.0 58-59 31.868468074244248 35.0 31.0 39.0 4.5 40.0 60-61 31.113964555300296 35.0 31.0 39.0 2.0 40.0 62-63 30.74638989169675 35.0 30.5 38.0 2.0 40.0 64-65 30.31985924224191 35.0 29.5 37.5 2.0 39.5 66-67 29.603307442657623 35.0 29.0 37.0 2.0 39.0 68-69 29.24118440724939 34.5 29.0 36.0 2.0 39.0 70-71 28.473744666885462 34.0 27.5 35.5 2.0 38.0 72-73 27.777267257411662 33.5 26.0 35.0 2.0 37.0 74-75 27.35045399846844 33.0 26.0 35.0 2.0 37.0 76-77 25.9613189658316 31.5 25.0 34.0 2.0 35.0 78-79 26.63109433687051 33.0 26.0 35.0 2.0 35.0 80-81 26.190460562301716 33.0 24.5 35.0 2.0 35.0 82-83 25.774796703497067 32.5 22.0 35.0 2.0 35.0 84-85 25.311207745323266 32.0 20.0 34.5 2.0 35.0 86-87 25.178171607774495 32.0 19.5 34.5 2.0 35.0 88-89 24.576687452138714 32.0 13.0 34.0 2.0 35.0 90-91 23.844099843197316 31.0 4.0 34.0 2.0 35.0 92-93 23.29863618130766 31.0 2.0 34.0 2.0 35.0 94-95 22.47872224045509 31.0 2.0 34.0 2.0 35.0 96-97 21.55910184881304 29.0 2.0 34.0 2.0 35.0 98-99 20.881486343580207 29.0 2.0 34.0 2.0 35.0 100-101 17.895133646938703 23.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.42739564166409494 1101 2 0.6773199042566382 1101 3 0.6381788512655859 1101 4 0.43271928236972457 1101 5 0.6482862384680388 1101 6 0.6400080435453646 1101 7 0.6502999883494809 1101 8 0.49855666436015156 1101 9 0.8928490385724928 1101 10-11 0.7740582617429297 1101 12-13 0.19136399255421566 1101 14-15 0.4140143127146345 1101 16-17 0.8463504112273128 1101 18-19 0.6727461561880617 1101 20-21 0.7775835207462976 1101 22-23 1.0262490705260419 1101 24-25 1.2664685626086936 1101 26-27 1.4126606434392244 1101 28-29 1.0035797339160624 1101 30-31 1.6152996043477614 1101 32-33 1.8543310149017884 1101 34-35 1.2383297991399616 1101 36-37 2.031844224184283 1101 38-39 2.3731316568912106 1101 40-41 2.659208895223351 1101 42-43 2.592275759504865 1101 44-45 2.13086984317831 1101 46-47 1.91006815887404 1101 48-49 1.8957605251821619 1101 50-51 1.4856069115211028 1101 52-53 1.9322544187823354 1101 54-55 1.5485850185165262 1101 56-57 1.525345619700012 1101 58-59 0.907129419364896 1101 60-61 1.104302499155395 1101 62-63 1.5597652081694058 1101 64-65 2.3208289572549035 1101 66-67 2.169473716262072 1101 68-69 2.088746066407218 1101 70-71 2.249233626492746 1101 72-73 2.6262161977156424 1101 74-75 2.946942213136726 1101 76-77 2.356085222261836 1101 78-79 2.0381625956723433 1101 80-81 1.6548882455621552 1101 82-83 2.034076910383849 1101 84-85 2.216875397086614 1101 86-87 1.2533701883234443 1101 88-89 1.7340893200868948 1101 90-91 2.2044734727891786 1101 92-93 3.0910105554062426 1101 94-95 3.43829052272282 1101 96-97 3.466073363309235 1101 98-99 3.5854943524665046 1101 100-101 4.2763190325193 1102 1 0.1737906259901152 1102 2 -0.05590893586875367 1102 3 0.45667414907123316 1102 4 0.19322084976784737 1102 5 -0.3792999684285192 1102 6 0.05223374887451371 1102 7 -0.2986028329671342 1102 8 0.38570399978335246 1102 9 0.23579574703644113 1102 10-11 0.2983529325893315 1102 12-13 0.13368374177051123 1102 14-15 0.8132306136550724 1102 16-17 0.028952292105053345 1102 18-19 -0.21377421998748503 1102 20-21 0.08996596588736594 1102 22-23 -0.3778261645836736 1102 24-25 -0.05955024616873317 1102 26-27 0.7668926183608491 1102 28-29 0.5153352198094865 1102 30-31 -0.07686340505662059 1102 32-33 -1.074979329925796 1102 34-35 -0.7130808591672491 1102 36-37 -0.9411965281668202 1102 38-39 -0.18987774749749775 1102 40-41 0.32347221810736215 1102 42-43 0.495567295555027 1102 44-45 1.0999921002315958 1102 46-47 1.4580305413191752 1102 48-49 0.975697829257399 1102 50-51 0.866954873903552 1102 52-53 0.18695661313970646 1102 54-55 0.6244470874820465 1102 56-57 1.0455650554366898 1102 58-59 0.74145543817367 1102 60-61 1.36386362768204 1102 62-63 1.2116460859123528 1102 64-65 0.9028038788850026 1102 66-67 0.826683747610037 1102 68-69 0.9337303924260283 1102 70-71 0.5639671688124928 1102 72-73 0.346278893640406 1102 74-75 1.2914563197197957 1102 76-77 1.3538908648950603 1102 78-79 -0.7875427334812599 1102 80-81 -0.22912429362279596 1102 82-83 0.05805810160642011 1102 84-85 0.1433644252997759 1102 86-87 1.151489310580498 1102 88-89 0.2536817965759255 1102 90-91 -0.06731336106662411 1102 92-93 -0.19911483644328598 1102 94-95 0.2718328424720369 1102 96-97 0.24318935077004866 1102 98-99 -0.016229785464531687 1102 100-101 1.6640933271901481 1103 1 0.3138390228443235 1103 2 -0.5861569697465505 1103 3 -0.11652609291304117 1103 4 0.39890748013687016 1103 5 0.3430230805732961 1103 6 0.10093308341777174 1103 7 0.11186298675458772 1103 8 9.490088577592815E-4 1103 9 0.25999417413867576 1103 10-11 0.5244569858258714 1103 12-13 -0.09045418926396565 1103 14-15 -0.4028915884017934 1103 16-17 -0.026855330399484956 1103 18-19 0.08598379574149106 1103 20-21 0.159082723298134 1103 22-23 -0.09288968545481424 1103 24-25 0.7441400139643619 1103 26-27 0.09447882525740425 1103 28-29 0.581729654171248 1103 30-31 0.014183176915551599 1103 32-33 -0.33163389737890725 1103 34-35 0.26544303677951575 1103 36-37 -0.1574699703930733 1103 38-39 0.2163533474813235 1103 40-41 0.03672085694583416 1103 42-43 0.5295963336675484 1103 44-45 0.8321457602436979 1103 46-47 1.118042640532721 1103 48-49 1.134197526777065 1103 50-51 0.67412365793259 1103 52-53 0.8126371939019492 1103 54-55 1.3661288781656467 1103 56-57 2.1969564649950613 1103 58-59 2.774274554931079 1103 60-61 2.2891509840038857 1103 62-63 2.6101639322523447 1103 64-65 2.186379196489355 1103 66-67 1.919393971445487 1103 68-69 1.4865132115427855 1103 70-71 1.1498715850254406 1103 72-73 1.2053230557698704 1103 74-75 1.6250921333919095 1103 76-77 1.0943627342235551 1103 78-79 1.4166314951938794 1103 80-81 1.6780142423723632 1103 82-83 1.3485904670026692 1103 84-85 0.865838714470982 1103 86-87 0.6608662010826158 1103 88-89 1.1742807076467017 1103 90-91 1.3095771410507382 1103 92-93 0.18367403228024415 1103 94-95 0.6236174764708231 1103 96-97 -0.8079298264834271 1103 98-99 -1.6180144194633215 1103 100-101 -0.8582902178794249 1104 1 0.11038684401013654 1104 2 -0.041448424189109545 1104 3 0.14521697443274206 1104 4 0.4206958219591712 1104 5 0.20635075459706798 1104 6 0.05056522829609378 1104 7 -0.01773520226635128 1104 8 0.05867396641293965 1104 9 0.46938862801530234 1104 10-11 0.426550930364634 1104 12-13 -0.2678735441026703 1104 14-15 -0.5774812884583866 1104 16-17 -0.01581967674923135 1104 18-19 0.23227694797692067 1104 20-21 0.23902047089293177 1104 22-23 0.22756871861988515 1104 24-25 0.8236533133418362 1104 26-27 0.804156244612237 1104 28-29 0.7389169773178281 1104 30-31 0.0076749709166819 1104 32-33 -0.2440560818724009 1104 34-35 0.06750868477046623 1104 36-37 -0.1539329019154252 1104 38-39 -0.6192437096777041 1104 40-41 -0.21879696987929265 1104 42-43 0.047847607012201365 1104 44-45 -0.3021213591677352 1104 46-47 -0.22717524288549384 1104 48-49 0.2154086190238047 1104 50-51 0.47930192618386513 1104 52-53 0.015098993562396856 1104 54-55 0.6147140507745874 1104 56-57 0.15819019448007055 1104 58-59 -0.8631051847406788 1104 60-61 0.3321617366920542 1104 62-63 1.205806263887883 1104 64-65 1.4164301302754367 1104 66-67 1.5260719567312826 1104 68-69 1.4896258318031101 1104 70-71 0.7641896382229483 1104 72-73 0.8162455232288401 1104 74-75 0.4838923597642939 1104 76-77 -0.40945729973229916 1104 78-79 -0.30450602602853394 1104 80-81 0.08010818691112931 1104 82-83 -0.43777059694753007 1104 84-85 -0.021541025200779274 1104 86-87 -0.2539611899756764 1104 88-89 0.39244709134789346 1104 90-91 0.6031538246953367 1104 92-93 0.5545002914766215 1104 94-95 0.7281732206700333 1104 96-97 0.7779501961538102 1104 98-99 0.42259112666005194 1104 100-101 1.8351166864782442 1105 1 0.09728200530046038 1105 2 -0.2848960048342697 1105 3 -0.042787057825321995 1105 4 -0.2652920812666366 1105 5 -0.27046376153196405 1105 6 -0.3330033200910023 1105 7 -0.257654557105063 1105 8 -0.24576151745802832 1105 9 0.10648540220885394 1105 10-11 -0.3317656018934301 1105 12-13 -0.5513916892639656 1105 14-15 -0.4693663691035468 1105 16-17 -0.38376322513632743 1105 18-19 -0.05779361653920745 1105 20-21 -0.20125170652642765 1105 22-23 -1.1348304749284992 1105 24-25 0.13010492624506043 1105 26-27 -0.48364617474259575 1105 28-29 0.0511649611887961 1105 30-31 -0.1415185774704142 1105 32-33 0.26526851490179126 1105 34-35 0.3235570718672349 1105 36-37 0.0035771787297349533 1105 38-39 -0.0013001612906080595 1105 40-41 0.19954980431425895 1105 42-43 0.032474623141233394 1105 44-45 -0.23432902045805548 1105 46-47 -0.4126591138532376 1105 48-49 0.0037150706367086173 1105 50-51 -0.3213533157516224 1105 52-53 -0.6018364903085711 1105 54-55 -0.12797748148347665 1105 56-57 0.5646922106091026 1105 58-59 0.011390783001257176 1105 60-61 0.4193593173372143 1105 62-63 0.5586288445330432 1105 64-65 0.153073275436725 1105 66-67 0.5734509889893467 1105 68-69 0.6282347027708539 1105 70-71 0.003352944674563929 1105 72-73 0.4588866522610964 1105 74-75 0.696089940409454 1105 76-77 1.396994313170925 1105 78-79 1.402651232035982 1105 80-81 0.9780416546530653 1105 82-83 0.8336507740202137 1105 84-85 0.7904549425411567 1105 86-87 0.11700655195980758 1105 88-89 1.0529813655414415 1105 90-91 0.4889905182437211 1105 92-93 -0.04933035368466676 1105 94-95 0.11854620454100129 1105 96-97 0.9069824542183262 1105 98-99 1.199556852466504 1105 100-101 1.9243303961556641 1106 1 0.4291272433957012 1106 2 0.19997502691176194 1106 3 0.3195641326508678 1106 4 -0.10234565269520601 1106 5 0.09907988926168798 1106 6 0.48272088625820686 1106 7 0.352464490513988 1106 8 0.4913416571451492 1106 9 0.14219968792313864 1106 10-11 0.43423142191609543 1106 12-13 0.36192676311698335 1106 14-15 0.17981517851550421 1106 16-17 0.6068121716890644 1106 18-19 0.3984315818734885 1106 20-21 0.7534853966481805 1106 22-23 0.6109532552302355 1106 24-25 1.2729620691021992 1106 26-27 0.9516216824002655 1106 28-29 0.6832334135697451 1106 30-31 0.6779258669740287 1106 32-33 0.8717913323621076 1106 34-35 0.661850722660887 1106 36-37 0.4022626946027472 1106 38-39 0.9406641244236766 1106 40-41 1.6444902805047406 1106 42-43 0.27813434536346193 1106 44-45 0.21643982874829248 1106 46-47 0.8770234258293073 1106 48-49 1.314230943652582 1106 50-51 0.6599214858356817 1106 52-53 1.078719065246986 1106 54-55 -0.46093879100728685 1106 56-57 0.0035562979106913417 1106 58-59 0.3548927671282449 1106 60-61 0.2335954284483286 1106 62-63 1.1786685270727268 1106 64-65 0.20726474369069336 1106 66-67 0.359661306449663 1106 68-69 -0.14631886865771904 1106 70-71 0.5324948097539313 1106 72-73 0.42044419194363414 1106 74-75 0.7027863689808846 1106 76-77 0.043819709996320455 1106 78-79 0.01897067648042494 1106 80-81 -0.2693541786802669 1106 82-83 0.6008878771948147 1106 84-85 0.5258220060332199 1106 86-87 0.7043081392613963 1106 88-89 0.1604962464938211 1106 90-91 0.4499280182437211 1106 92-93 1.4660466304423174 1106 94-95 1.6169341013664003 1106 96-97 1.33952213675801 1106 98-99 1.6981927651649151 1106 100-101 2.1621776183778856 1107 1 0.043491021693903065 1107 2 0.4141306345099913 1107 3 0.5389240077484487 1107 4 0.5838370170940195 1107 5 0.44828623846803595 1107 6 0.1616175815483416 1107 7 0.3212388855178858 1107 8 0.4150991382796789 1107 9 -0.028760499430489972 1107 10-11 0.5333727177786969 1107 12-13 0.3931523681130784 1107 14-15 1.0092453112243263 1107 16-17 0.9231015289620288 1107 18-19 0.003297571985378056 1107 20-21 0.06014685904734307 1107 22-23 -0.4306808847645627 1107 24-25 -0.4764524508041177 1107 26-27 -0.012078551791773862 1107 28-29 -0.21996721094235028 1107 30-31 -0.16815792173270694 1107 32-33 -0.10960341132771134 1107 34-35 -0.21025440354259928 1107 36-37 -1.105413599958787 1107 38-39 -0.49259114489716893 1107 40-41 -1.0694051137185312 1107 42-43 -0.3375304998095885 1107 44-45 -1.2393238975072336 1107 46-47 -0.9823312450007791 1107 48-49 -1.5423914867403425 1107 50-51 -0.6498625370631004 1107 52-53 -0.5178200968659468 1107 54-55 -0.3399395716474132 1107 56-57 0.47363175978943417 1107 58-59 -0.662021102244644 1107 60-61 -0.22177797774475394 1107 62-63 0.13547310682812608 1107 64-65 -0.4577105360386824 1107 66-67 -0.8348481913385193 1107 68-69 -0.017513248048821595 1107 70-71 0.536267903690959 1107 72-73 0.5283026358676537 1107 74-75 -0.31594899401677523 1107 76-77 -0.6234974901077628 1107 78-79 -0.4336704892754959 1107 80-81 -0.6901192060026737 1107 82-83 -0.8219588571273277 1107 84-85 -0.6442274345080214 1107 86-87 -0.8256163988598644 1107 88-89 -1.4171774459339694 1107 90-91 -1.1812195227398838 1107 92-93 -1.4525065831928643 1107 94-95 -1.9684158856229352 1107 96-97 -0.46596836545380427 1107 98-99 -0.5505712213039864 1107 100-101 0.46454556009009096 1108 1 -0.36634504387986766 1108 2 -0.04488575893262947 1108 3 -0.08402681192368178 1108 4 0.04285341053664382 1108 5 0.4646796810909848 1108 6 0.3911257782696538 1108 7 -0.1705643931706362 1108 8 0.2511647120501692 1108 9 0.05320671368426133 1108 10-11 0.025175996467226014 1108 12-13 0.18823433532620015 1108 14-15 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0.8565898852931397 2316 88-89 0.11287719887477721 2316 90-91 -0.2688219817562789 2316 92-93 -1.5389136870180025 2316 94-95 -1.321557962125663 2316 96-97 -0.15551754578167376 2316 98-99 -1.1728389808668283 2316 100-101 0.8670387294889963 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 3.0 5 4.0 6 32.0 7 48.0 8 57.0 9 77.0 10 101.0 11 212.0 12 228.0 13 382.0 14 438.0 15 609.0 16 752.0 17 973.0 18 929.0 19 735.0 20 717.0 21 747.0 22 810.0 23 782.0 24 894.0 25 991.0 26 1117.0 27 1222.0 28 1420.0 29 1644.0 30 1951.0 31 2287.0 32 2787.0 33 3439.0 34 4935.0 35 6721.0 36 7102.0 37 7482.0 38 2190.0 39 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.03255220206073 21.56287042947023 18.655967903711137 10.74860946475791 2 14.132555383353084 31.047497492934635 31.658309782113232 23.16163734159905 3 29.750574335411876 27.427706669583923 34.073587864201585 8.74813113080261 4 10.670070197830249 22.11505150879752 41.161455009572435 26.0534232837998 5 7.787258870291361 44.25299930715093 35.40094081610327 12.558801006454438 6 20.84381723370893 39.419465412245195 16.074098384567698 23.662618969478174 7 16.76694745286803 41.49618933012435 24.147613317288407 17.589249899719213 8 21.356160886846805 25.05378696714437 22.158407176457718 31.431644969551108 9 15.919118987711045 28.61831309484739 29.526310031725195 25.93625788571637 10-11 23.652443471918307 26.420495988329684 25.62180889861415 24.305251641137858 12-13 32.326878897276 27.00105750647267 25.141304744192833 15.53075885205849 14-15 22.579586478503447 31.71334281442585 34.295117237355505 11.411953469715202 16-17 14.197024395580351 33.811034533056194 33.44181891113299 18.550122160230465 18-19 13.502352040258177 38.04561864128651 32.476388433067136 15.975640885388179 20-21 18.76344674178609 29.478904569157276 29.491667578310178 22.265981110746456 22-23 15.075486835387645 30.396214718109547 34.03015826708482 20.498140179417987 24-25 22.82773208847718 30.910483415087803 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50-51 11.819889140106483 34.052950185982056 38.81007949821311 15.317081175698345 52-53 17.283849323560517 36.761112934398135 28.640192539109506 17.314845202931846 54-55 13.555968856554163 30.59916488886458 32.97868460879237 22.86618164578889 56-57 20.321460154804125 29.307939865252948 30.293471423231562 20.07712855671137 58-59 24.996353425956315 28.94832804580097 29.8107428071327 16.244575721110017 60-61 17.615848155272538 32.61981384069796 36.41593202724015 13.348405976789346 62-63 14.997720849667246 32.39857781019236 34.783480718388184 17.82022062175221 64-65 14.337417496262262 38.34281442584692 31.120774532326877 16.19899354556394 66-67 17.64791685659586 30.834169021788675 28.405506427203935 23.112407694411523 68-69 14.432228421398097 31.695109944207417 32.65871713525143 21.213944499143057 70-71 21.99248805747001 31.48907851073916 27.872588702913614 18.64584472887722 72-73 18.39331947635197 26.673777486051854 37.0865696677971 17.846333369799076 74-75 14.176968238340079 37.44028735003464 30.423367246471937 17.959377165153338 76-77 17.026765853480654 29.215439594500964 29.95751741239106 23.800277139627322 78-79 17.48199471237123 30.29993618379068 33.251891694776184 18.966177409061903 80-81 20.773277174557155 29.378515621438794 30.27650903919263 19.571698164811423 82-83 16.480370525701574 28.150471362666618 36.25480935796211 19.1143487536697 84-85 15.835825249799433 31.07632557800306 32.80577638392532 20.282072788272192 86-87 16.178615710013858 32.64258624462111 30.17103055940486 21.007767485960176 88-89 15.80516354884586 31.870145498304343 26.929949312620792 25.394741640229007 90-91 18.45274452780994 26.50354176732822 29.584013273650527 25.459700431211314 92-93 20.81190971082668 31.47722714509718 30.30941180760675 17.401451336469385 94-95 18.415198920614085 30.024067388688323 29.942931116216315 21.617802574481274 96-97 11.12296247675309 31.655909273237793 35.049958064398496 22.17117018561062 98-99 16.96599507703528 33.64299389187711 25.757133740541526 23.633877290546085 100-101 14.737628997556795 29.782481858294137 28.436896036174016 27.042993107975054 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3360.0 1 2055.5 2 644.5 3 503.5 4 405.5 5 375.0 6 496.0 7 815.5 8 1040.0 9 929.0 10 650.5 11 411.0 12 327.5 13 290.0 14 261.5 15 223.5 16 195.0 17 193.0 18 188.0 19 176.5 20 176.5 21 184.0 22 192.0 23 202.5 24 217.0 25 237.0 26 262.5 27 300.5 28 313.5 29 318.5 30 362.5 31 390.0 32 373.5 33 374.5 34 396.5 35 397.5 36 377.0 37 315.5 38 267.0 39 247.0 40 228.5 41 229.0 42 247.0 43 293.5 44 383.0 45 512.0 46 1049.0 47 4496.5 48 11386.0 49 10800.5 50 4106.0 51 1294.5 52 483.5 53 250.0 54 210.5 55 193.5 56 150.5 57 125.0 58 122.5 59 108.5 60 93.0 61 86.0 62 65.5 63 39.5 64 24.5 65 19.0 66 20.0 67 19.0 68 14.0 69 7.5 70 8.0 71 7.0 72 3.5 73 1.5 74 0.5 75 0.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02005615724027276 2 0.0018232870218429784 3 0.0 4 0.0018232870218429784 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.01093972213105787 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.003646574043685957 24-25 0.012763009152900849 26-27 0.007293148087371914 28-29 0.0 30-31 0.005469861065528935 32-33 0.008204791598293402 34-35 0.0 36-37 0.008204791598293402 38-39 0.013674652663822338 40-41 0.0018232870218429784 42-43 0.0 44-45 0.0 46-47 0.0 48-49 9.116435109214892E-4 50-51 0.003646574043685957 52-53 0.0 54-55 0.005469861065528935 56-57 0.004558217554607446 58-59 0.0 60-61 9.116435109214892E-4 62-63 0.0018232870218429784 64-65 0.0 66-67 0.0018232870218429784 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0018232870218429784 80-81 0.0027349305327644675 82-83 0.009116435109214891 84-85 0.003646574043685957 86-87 0.003646574043685957 88-89 0.0 90-91 9.116435109214892E-4 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0018232870218429784 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 54846.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.92488057470007 #Duplication Level Percentage of deduplicated Percentage of total 1 83.14421669106882 16.566385880465305 2 8.455344070278183 3.369434416365824 3 2.635431918008785 1.5753199868723335 4 1.390922401171303 1.1085585092805308 5 0.722913616398243 0.7201983736279765 6 0.5307467057101025 0.6345038836013566 7 0.32027818448023426 0.4467053203515297 8 0.2745241581259151 0.4375888852423148 9 0.2745241581259151 0.4922874958976042 >10 1.4824304538799415 5.6467199066477045 >50 0.2562225475841874 3.808846588629982 >100 0.3019765739385066 12.338183276811437 >500 0.1281112737920937 19.04240965612807 >1k 0.08235724743777453 33.81285782007804 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3966 7.231156328629253 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3176 5.790759581373299 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3106 5.663129489844292 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2132 3.88724793056923 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1571 2.8643839113153193 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1268 2.311927943696897 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1230 2.2426430368668635 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1058 1.9290376691098714 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1038 1.8925719286730116 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 992 1.8087007256682348 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 943 1.7193596615979287 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 939 1.7120665135105568 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 873 1.5917295700689202 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 834 1.5206213762170442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 813 1.4823323487583415 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 800 1.4586296174743827 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 695 1.26718448018087 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 688 1.2544214710279693 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 642 1.1705502680231923 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 594 1.083032490974729 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 581 1.0593297596907705 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 539 0.9827517047733655 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 511 0.9316996681617621 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 417 0.7603106881085221 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 411 0.7493709659774641 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 406 0.7402545308682493 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 366 0.6673230499945302 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 351 0.6399737446668855 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 321 0.5852751340115961 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 275 0.5014039310068191 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 254 0.4631149035481165 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 219 0.39929985778361227 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 212 0.38653684863071147 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 205 0.3737738394778106 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 205 0.3737738394778106 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 198 0.36101083032490977 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 195 0.35554096925938083 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 186 0.33913138606279397 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 181 0.33001495095357913 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 176 0.3208985158443642 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 174 0.3172519418006783 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 173 0.3154286547788353 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 172 0.3136053677569923 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 163 0.2971957845604055 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 156 0.28443277540750467 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 156 0.28443277540750467 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 149 0.2716697662546038 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 144 0.26255333114538887 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 131 0.2388505998614302 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 128 0.23338073879590124 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 125 0.22791087773037233 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 106 0.19326842431535574 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 106 0.19326842431535574 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 103 0.1877985632498268 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 102 0.18597527622798382 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 101 0.18415198920614081 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 100 0.18232870218429784 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 100 0.18232870218429784 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 97 0.1768588411187689 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 0.17503555409692592 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 94 0.17138898005324 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 91 0.16591911898771106 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 91 0.16591911898771106 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89 0.1622725449440251 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 88 0.1604492579221821 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 88 0.1604492579221821 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 88 0.1604492579221821 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 84 0.15315610983481018 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 71 0.12945337855085148 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 69 0.12580680450716553 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 68 0.12398351748532253 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 68 0.12398351748532253 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 65 0.1185136564197936 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 65 0.1185136564197936 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 64 0.11669036939795062 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 62 0.11304379535426468 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 62 0.11304379535426468 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 62 0.11304379535426468 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 56 0.10210407322320679 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 56 0.10210407322320679 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 55 0.10028078620136383 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0018232870218429784 0.0 20-21 0.0 0.0 0.0 0.0018232870218429784 0.0 22-23 0.0 0.0 0.0 0.0027349305327644675 0.0 24-25 0.0 0.0 0.0 0.006381504576450425 0.0 26-27 0.0 0.0 0.0 0.02643766181672319 0.0 28-29 0.0 0.0 0.0 0.0683732633191117 0.0 30-31 0.0 0.0 0.0 0.11304379535426468 0.0 32-33 0.0 0.0 0.0 0.15042117930204574 0.0 34-35 0.0 0.0 0.0 0.19144513729351276 0.0 36-37 0.0 0.0 0.0 0.22061772964300042 0.0 38-39 0.0 0.0 0.0 0.298107428071327 0.0 40-41 0.0 0.0 0.0 0.37833205703241807 0.0 42-43 0.0 0.0 0.0 0.4649381905699595 0.0 44-45 0.0 0.0 0.0 0.49502242643036865 0.0 46-47 0.0 0.0 0.0 0.5424278889982861 0.0 48-49 0.0 0.0 0.0 0.5889217080552821 0.0 50-51 0.0 0.0 0.0 0.637238814134121 0.0 52-53 0.0 0.0 0.0 0.7165517995842906 0.0 54-55 0.0 0.0 0.0 0.7776319148160304 0.0 56-57 0.0 0.0 0.0 0.9353462422054479 0.0 58-59 0.0 0.0 0.0 1.1176749443897458 0.0 60-61 0.0 0.0 0.0 1.2817707763556139 0.0 62-63 0.0 0.0 0.0 1.3729351274477628 0.0 64-65 0.0 0.0 0.0 1.5434124639900815 0.0 66-67 0.0 0.0 0.0 1.8451664661050942 0.0 68-69 0.0 0.0 0.0 2.2845786383692523 0.0 70-71 0.0 0.0 0.0 2.651970973270612 0.0 72-73 0.0 0.0 0.0 3.0257448127484228 0.0 74-75 0.0 0.0 0.0 3.555409692593808 0.0 76-77 0.0 0.0 0.0 3.799730153520767 0.0 78-79 0.0 0.0 0.0 4.25464026547059 0.0 80-81 0.0 0.0 0.0 4.784305145315976 0.0 82-83 0.0 0.0 0.0 5.1061153046712615 0.0 84-85 0.0 0.0 0.0 5.668599350909821 0.0 86-87 0.0 0.0 0.0 6.327717609306057 0.0 88-89 0.0 0.0 0.0 7.161871421799219 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 125 0.0 45.59124 9 AAGCAGG 40 2.2410495E-7 41.554516 94-95 CATGGGG 50 0.0016039335 37.992702 6 CTTTGCG 75 3.044059E-4 31.660585 2 AACACAC 30 0.0039392137 31.660583 90-91 GCTTTGC 80 4.443636E-4 29.681797 1 TTTGCGT 90 8.848755E-4 26.383821 3 TTGCGTT 95 0.0012130653 24.995197 4 CAGAAAA 135 1.8189894E-12 24.624899 10-11 GAGAAAA 95 3.2370917E-7 22.495678 12-13 CTGCTTT 110 0.0028460922 21.586761 9 GAGTACT 535 0.0 20.860477 12-13 GTACTTT 555 0.0 19.680904 14-15 GCAGAGA 200 1.8189894E-12 18.996351 94-95 AGTACTT 515 0.0 18.904135 12-13 CAGAGAA 115 2.0272328E-6 18.583385 10-11 ACTTTTT 605 0.0 18.446869 16-17 TACTTTT 570 0.0 17.080051 14-15 AGAGAAA 105 3.9677878E-4 15.830292 12-13 GCAGAAA 185 4.825779E-8 15.402447 10-11 >>END_MODULE