##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936768_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 55695 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.760768471137446 31.0 31.0 34.0 30.0 34.0 2 31.836107370500045 31.0 31.0 34.0 30.0 34.0 3 31.907909148038424 31.0 31.0 34.0 30.0 34.0 4 35.58800610467726 37.0 35.0 37.0 33.0 37.0 5 35.41352006463776 37.0 35.0 37.0 33.0 37.0 6 35.6332525361343 37.0 35.0 37.0 33.0 37.0 7 35.62826106472753 37.0 35.0 37.0 33.0 37.0 8 35.635083939312324 37.0 35.0 37.0 33.0 37.0 9 37.13898913726546 39.0 37.0 39.0 33.0 39.0 10-11 37.07152347607505 39.0 37.0 39.0 33.0 39.0 12-13 37.060202890744236 39.0 37.0 39.0 33.0 39.0 14-15 37.81038692880869 39.0 37.0 41.0 32.5 41.0 16-17 37.804982493940216 39.0 37.0 41.0 32.5 41.0 18-19 37.72567555435856 39.0 35.5 41.0 33.0 41.0 20-21 37.68978364305593 39.0 35.5 41.0 33.0 41.0 22-23 37.5640362689649 39.0 35.0 41.0 33.0 41.0 24-25 37.31009964987881 39.0 35.0 41.0 32.5 41.0 26-27 36.87188257473741 38.5 35.0 40.5 32.0 41.0 28-29 36.710458748541164 38.0 35.0 40.0 31.5 41.0 30-31 36.623440165185386 38.0 35.0 40.0 31.5 41.0 32-33 36.23255229374271 38.0 35.0 40.0 31.0 41.0 34-35 35.9100996498788 38.0 35.0 40.0 30.5 41.0 36-37 35.621070114013826 37.5 35.0 40.0 30.0 41.0 38-39 35.3605171020738 37.0 35.0 40.0 30.0 41.0 40-41 35.14688033037077 37.0 35.0 40.0 28.5 41.0 42-43 34.837058981955295 37.0 34.5 40.0 27.0 41.0 44-45 34.54228386749259 37.0 34.0 40.0 25.0 41.0 46-47 34.04658407397432 37.0 33.0 40.0 23.5 41.0 48-49 33.45521141933746 36.0 33.0 40.0 22.0 41.0 50-51 33.03175329921896 36.0 33.0 40.0 20.0 41.0 52-53 32.92424813717569 36.0 33.0 40.0 19.5 41.0 54-55 32.557599425442135 35.5 32.5 40.0 14.0 41.0 56-57 32.09417362420325 35.0 31.5 39.5 10.0 40.5 58-59 31.73826196247419 35.0 31.0 39.0 3.5 40.0 60-61 30.9260166980878 35.0 31.0 38.5 2.0 40.0 62-63 30.577556333602658 35.0 30.0 38.0 2.0 40.0 64-65 30.25438549241404 35.0 29.5 37.5 2.0 39.5 66-67 29.702343118771882 35.0 29.0 37.0 2.0 39.0 68-69 29.05803034383697 34.0 28.5 36.0 2.0 39.0 70-71 28.26065176407218 33.5 27.0 35.5 2.0 37.5 72-73 27.690681389711823 33.0 26.0 35.0 2.0 37.0 74-75 27.334868480114913 33.0 26.0 35.0 2.0 36.5 76-77 25.882897926205224 31.5 25.0 34.0 2.0 35.0 78-79 26.5018583355777 33.0 25.5 34.5 2.0 35.0 80-81 26.134258012388905 33.0 25.0 35.0 2.0 35.0 82-83 25.73400664332525 32.5 22.0 35.0 2.0 35.0 84-85 25.21595295807523 32.0 20.0 34.0 2.0 35.0 86-87 25.05009426339887 32.0 19.5 34.5 2.0 35.0 88-89 24.458658766496093 32.0 12.0 34.0 2.0 35.0 90-91 23.691713798366102 31.0 3.5 34.0 2.0 35.0 92-93 23.13397971092558 31.0 2.0 34.0 2.0 35.0 94-95 22.35912559475716 30.0 2.0 34.0 2.0 35.0 96-97 21.38630936349762 29.0 2.0 34.0 2.0 35.0 98-99 20.698994523745398 29.0 2.0 34.0 2.0 35.0 100-101 17.973929437112847 23.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10762816128698915 1101 2 0.482466497340166 1101 3 0.4268543956770827 1101 4 0.5976319943752131 1101 5 -0.06115988445889542 1101 6 0.20868690067620577 1101 7 0.5611188765734738 1101 8 0.6476652397436169 1101 9 0.20053733224611392 1101 10-11 0.6955566110185316 1101 12-13 0.7633970474901872 1101 14-15 0.9399702452678227 1101 16-17 0.783879697847695 1101 18-19 1.0472781438632381 1101 20-21 0.7033328438595206 1101 22-23 1.3788234844754328 1101 24-25 1.2337558671094158 1101 26-27 1.49210128884107 1101 28-29 1.4221264839782108 1101 30-31 1.840270791983734 1101 32-33 2.257443363079034 1101 34-35 1.495240803410688 1101 36-37 1.8061485480706523 1101 38-39 2.4178487488302594 1101 40-41 2.798033589153242 1101 42-43 2.2539033296161293 1101 44-45 2.066188287614658 1101 46-47 2.2904576094504705 1101 48-49 2.3719286404767743 1101 50-51 2.3970098194118137 1101 52-53 2.962042858100368 1101 54-55 2.4638438969329 1101 56-57 2.8172209748575874 1101 58-59 3.0003267213235993 1101 60-61 3.774227719304651 1101 62-63 4.000259608048239 1101 64-65 3.6399447141040646 1101 66-67 4.276424237361081 1101 68-69 4.033801596318963 1101 70-71 2.995829355472946 1101 72-73 2.796615055240011 1101 74-75 2.687125371814677 1101 76-77 2.8653677579812253 1101 78-79 2.991512223333288 1101 80-81 3.556758451995556 1101 82-83 2.655567087609654 1101 84-85 2.047360154210825 1101 86-87 2.1670162649806635 1101 88-89 2.7636647059175523 1101 90-91 3.6764877487140666 1101 92-93 4.066301463957849 1101 94-95 4.024811035779944 1101 96-97 3.418054986192555 1101 98-99 4.1787108563478945 1101 100-101 3.603318698231533 1102 1 -0.15216622604282293 1102 2 -0.09963964325444508 1102 3 -0.27445152263355865 1102 4 0.21447000493370183 1102 5 -0.27094037904072366 1102 6 0.049476008761779156 1102 7 -0.32996197921980297 1102 8 0.4220470124582576 1102 9 0.04271571513024952 1102 10-11 -0.04951146344659918 1102 12-13 0.2061589257897154 1102 14-15 -0.19687332238985533 1102 16-17 -0.7415440309658621 1102 18-19 0.08214894130696848 1102 20-21 0.7658502097945004 1102 22-23 0.2210018673595684 1102 24-25 -0.34251531933126245 1102 26-27 0.48459920492886965 1102 28-29 0.43393531976592925 1102 30-31 0.8273505363571658 1102 32-33 0.09114716969198611 1102 34-35 1.3524233541192174 1102 36-37 0.8683880590459196 1102 38-39 1.4586934279077823 1102 40-41 1.9611344505036215 1102 42-43 1.0073070528725907 1102 44-45 0.8958631973117974 1102 46-47 1.3072678345129916 1102 48-49 1.8692890183595168 1102 50-51 0.8925641400564359 1102 52-53 0.11013955162633948 1102 54-55 0.8949358669245555 1102 56-57 -0.5456576025250186 1102 58-59 0.19065736872565608 1102 60-61 0.5976508924082928 1102 62-63 0.4082896163838825 1102 64-65 0.1452239583385655 1102 66-67 0.6344403529487472 1102 68-69 -0.4336893733948486 1102 70-71 0.4442872604465471 1102 72-73 -0.6050242890222819 1102 74-75 -0.1995098046232222 1102 76-77 0.18142777465251925 1102 78-79 0.22268754981286776 1102 80-81 0.8883783464106685 1102 82-83 0.8963284101992635 1102 84-85 1.071533535705683 1102 86-87 0.0692113191623811 1102 88-89 0.21156690097173225 1102 90-91 0.2762376792503254 1102 92-93 0.004339807942290719 1102 94-95 -1.14259657744595 1102 96-97 -0.5733315100564056 1102 98-99 -0.6472130113931449 1102 100-101 -0.8195765504486552 1103 1 0.013498071387466837 1103 2 0.39653312875211455 1103 3 0.14763705035714736 1103 4 0.2073622958030299 1103 5 0.3693001895760091 1103 6 -0.013946626558066555 1103 7 -0.44231845817004967 1103 8 -0.019177700499639627 1103 9 -0.41791851122295043 1103 10-11 0.03796803354629219 1103 12-13 0.08901841184767534 1103 14-15 0.36395226536301806 1103 16-17 0.300008730105759 1103 18-19 0.3089121999182396 1103 20-21 -0.08447783831922351 1103 22-23 0.06681925390959265 1103 24-25 0.298271996141672 1103 26-27 1.3101869577992886 1103 28-29 1.0818303443695356 1103 30-31 0.39883768780604356 1103 32-33 -0.06262538361583836 1103 34-35 0.11158136396065998 1103 36-37 -0.00572347731274192 1103 38-39 0.946801683785317 1103 40-41 1.2284936631881465 1103 42-43 1.230106396776364 1103 44-45 0.5343268386458533 1103 46-47 1.0122979055900814 1103 48-49 0.8127007187422421 1103 50-51 1.108802521685739 1103 52-53 0.3593194313420369 1103 54-55 0.6498565886304135 1103 56-57 -0.2601190568716554 1103 58-59 0.6758952036081034 1103 60-61 0.7251795966182435 1103 62-63 0.9149872653724032 1103 64-65 1.306787654347314 1103 66-67 1.7310778597830812 1103 68-69 0.8005916544892422 1103 70-71 1.7905147071387297 1103 72-73 1.999814420655138 1103 74-75 1.9960888831843242 1103 76-77 1.1464359758553648 1103 78-79 1.0024852534760669 1103 80-81 0.225584469975459 1103 82-83 0.8192371034742756 1103 84-85 1.043922819467305 1103 86-87 0.3425847472651675 1103 88-89 0.550549951819189 1103 90-91 -0.1451674601701214 1103 92-93 -0.7832490384218467 1103 94-95 -0.7785998579270874 1103 96-97 1.212343722311008 1103 98-99 0.8741101159988744 1103 100-101 1.820664018168074 1104 1 0.16387027982419156 1104 2 0.3699822602657612 1104 3 0.3615328319948645 1104 4 -0.04524316325404243 1104 5 -0.3880199096795778 1104 6 0.020792827536226355 1104 7 0.19415796862895718 1104 8 0.38032602158472884 1104 9 -0.08218650129739302 1104 10-11 -0.3595505768755416 1104 12-13 0.06730625303873694 1104 14-15 0.11147087578485326 1104 16-17 0.3684950824630846 1104 18-19 0.3147434654269148 1104 20-21 0.7129167026237084 1104 22-23 -0.019284964452687348 1104 24-25 -0.15855182519828048 1104 26-27 0.09033584117398163 1104 28-29 0.8929965974712744 1104 30-31 0.0021875637365695866 1104 32-33 0.3208733756893736 1104 34-35 0.4929709421244226 1104 36-37 0.8008522050693969 1104 38-39 0.4667768698895358 1104 40-41 0.8019427947017945 1104 42-43 0.5092627243197825 1104 44-45 1.244624605395238 1104 46-47 1.0445559701062095 1104 48-49 1.576720569858871 1104 50-51 2.05123428347234 1104 52-53 1.708575014468586 1104 54-55 1.8043230898711045 1104 56-57 2.545215930721401 1104 58-59 2.824034161424482 1104 60-61 2.740936420439585 1104 62-63 2.451835900608131 1104 64-65 3.7534377784167994 1104 66-67 2.2957180582942485 1104 68-69 1.6747852028763397 1104 70-71 1.8740134664439445 1104 72-73 1.533437249439256 1104 74-75 0.6890169228865552 1104 76-77 0.8839049584856369 1104 78-79 1.4333785537242107 1104 80-81 0.8239715667496519 1104 82-83 1.7877234558315962 1104 84-85 0.8188607847278497 1104 86-87 2.221492935850776 1104 88-89 1.458242259511497 1104 90-91 0.9184801825097786 1104 92-93 0.5018626241091724 1104 94-95 0.7671817797900324 1104 96-97 0.3793412409214305 1104 98-99 1.1473111085547067 1104 100-101 1.6171900727586461 1105 1 -0.19395465386281074 1105 2 -0.46609201029126623 1105 3 -0.5225520486417388 1105 4 -0.20253759296226548 1105 5 0.48943367122227954 1105 6 -0.025626270607006063 1105 7 0.003176536268213681 1105 8 -0.07590740016593855 1105 9 0.32179227854345527 1105 10-11 0.5457544629122566 1105 12-13 0.32261129282653656 1105 14-15 -0.46279968124432713 1105 16-17 0.05974176681321097 1105 18-19 0.03583099858342109 1105 20-21 -0.09782600294659005 1105 22-23 -0.34474915543412266 1105 24-25 0.24237655676459013 1105 26-27 -0.44175964954219893 1105 28-29 -0.4951996890008772 1105 30-31 -0.7009954601361059 1105 32-33 -1.07819824234776 1105 34-35 -1.8692306493875108 1105 36-37 -1.9178480601825996 1105 38-39 -1.916379628784206 1105 40-41 -1.099648717234551 1105 42-43 -0.7818513340356503 1105 44-45 -1.1333594794853994 1105 46-47 -0.6709612712731001 1105 48-49 -1.395030093271103 1105 50-51 -1.5925322947769942 1105 52-53 -0.6481889112608528 1105 54-55 0.17766526493741708 1105 56-57 0.11696659385136954 1105 58-59 -0.5885653080715407 1105 60-61 -0.9133076113906533 1105 62-63 -1.8555911550947854 1105 64-65 -1.8778619563312162 1105 66-67 -3.0178097931646377 1105 68-69 -2.0890079005719393 1105 70-71 -2.5871271171104375 1105 72-73 -2.484334633849869 1105 74-75 -2.898516233612117 1105 76-77 -2.260126871753087 1105 78-79 -1.6126426664349438 1105 80-81 -2.001890502215865 1105 82-83 -2.7486160667148205 1105 84-85 -4.139892530922019 1105 86-87 -3.456358523035167 1105 88-89 -4.027036255077363 1105 90-91 -3.507514512450431 1105 92-93 -3.235140028410722 1105 94-95 -3.0108023050906034 1105 96-97 -3.5515606158424937 1105 98-99 -2.5599833211535206 1105 100-101 -0.7574783622546164 1106 1 -0.5928441921445184 1106 2 -0.06574133816970118 1106 3 -0.3761464378877939 1106 4 -0.07366558828663727 1106 5 0.017195214179615448 1106 6 -0.0013714488653349122 1106 7 -0.1435213012537062 1106 8 -0.0016818011010641953 1106 9 -0.025080895039245377 1106 10-11 -0.2952741753110075 1106 12-13 0.2654809596880199 1106 14-15 0.014991084389805565 1106 16-17 -0.3432389462200973 1106 18-19 -0.4771730925913289 1106 20-21 -0.6324548749512644 1106 22-23 -0.6349303360302585 1106 24-25 -0.18149837017872272 1106 26-27 -0.049299100155877795 1106 28-29 0.1119014214608427 1106 30-31 0.8358251126283562 1106 32-33 0.5996217459631694 1106 34-35 0.02191487954294047 1106 36-37 0.6819473810798229 1106 38-39 0.9163205465518516 1106 40-41 0.9357107216900644 1106 42-43 0.40561213761834836 1106 44-45 -0.36684866709498465 1106 46-47 -0.014766063792094997 1106 48-49 0.5472551200544373 1106 50-51 1.0112082078530449 1106 52-53 1.28810565332126 1106 54-55 0.9203595957381197 1106 56-57 1.2085796856105766 1106 58-59 1.1482844873697253 1106 60-61 1.2247695364760887 1106 62-63 -0.2442527564974739 1106 64-65 0.4757324329148389 1106 66-67 0.7361352682029825 1106 68-69 0.49851401643566007 1106 70-71 0.4442872604465471 1106 72-73 0.8356536770794136 1106 74-75 1.6225240936818608 1106 76-77 1.0966820119406577 1106 78-79 1.4769248379484594 1106 80-81 1.5239715667496512 1106 82-83 2.7268368847755333 1106 84-85 2.520686078078569 1106 86-87 2.204804539501364 1106 88-89 1.2200414772429191 1106 90-91 1.072847848741855 1106 92-93 2.156882180823647 1106 94-95 2.1624881683167594 1106 96-97 1.4181939136724075 1106 98-99 2.1070242767424467 1106 100-101 1.697372602093715 1107 1 0.058447833418139084 1107 2 0.4332861694713124 1107 3 0.27931341207052895 1107 4 -0.22204013677232837 1107 5 -0.5365697205244686 1107 6 0.028359031823747216 1107 7 0.3643975650980593 1107 8 0.18864884630099255 1107 9 0.5284061847051262 1107 10-11 0.3103107093791877 1107 12-13 0.39454458847379215 1107 14-15 0.05472434362848588 1107 16-17 0.2183059273558925 1107 18-19 -0.18223005285807403 1107 20-21 0.006611532384113161 1107 22-23 -0.5064224171639111 1107 24-25 -0.553129378792228 1107 26-27 -0.18822658001138848 1107 28-29 -0.8319718766775281 1107 30-31 -0.1023521588359344 1107 32-33 -0.07862221069145647 1107 34-35 -0.2260706719991532 1107 36-37 -0.07090063225721366 1107 38-39 -0.03297092330088702 1107 40-41 0.44557457275980283 1107 42-43 -0.024785194974029423 1107 44-45 0.13995877941793822 1107 46-47 -0.14396862005772704 1107 48-49 0.25717454211612534 1107 50-51 -0.8324983773094985 1107 52-53 0.4784363007233168 1107 54-55 -0.14271348011628504 1107 56-57 0.2762373683002117 1107 58-59 0.09868737706130304 1107 60-61 -0.3241329364330525 1107 62-63 -0.21285514605012423 1107 64-65 0.14814143541553548 1107 66-67 0.0469160406397684 1107 68-69 -0.7448869282712032 1107 70-71 -0.35662966092049686 1107 72-73 -0.3263357644321161 1107 74-75 -0.13254675933286464 1107 76-77 -0.5690584715269722 1107 78-79 -0.5576681045355656 1107 80-81 -1.1235694168569061 1107 82-83 -1.7952525845214922 1107 84-85 -1.3870660752973691 1107 86-87 -0.6034755382980244 1107 88-89 -0.957646769492289 1107 90-91 -1.56121716931872 1107 92-93 -2.245173945878218 1107 94-95 -2.1883037183184157 1107 96-97 -1.590141735118923 1107 98-99 -1.4524366846357104 1107 100-101 -1.1835665476701074 1108 1 -0.38360982627660434 1108 2 0.06839074832942416 1108 3 -0.5742761865727743 1108 4 0.056083096692908896 1108 5 0.19633022294642188 1108 6 -0.4566607533656253 1108 7 -0.3761338085593735 1108 8 -0.48970050361421613 1108 9 0.028688830267590504 1108 10-11 -0.014590364673949807 1108 12-13 -0.14290594855277305 1108 14-15 -0.5662479571063912 1108 16-17 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98-99 -3.7546119683683727 2316 100-101 -4.757809927241356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 2.0 5 7.0 6 40.0 7 61.0 8 55.0 9 97.0 10 144.0 11 187.0 12 267.0 13 365.0 14 487.0 15 631.0 16 789.0 17 994.0 18 888.0 19 771.0 20 683.0 21 751.0 22 812.0 23 866.0 24 945.0 25 1030.0 26 1118.0 27 1232.0 28 1490.0 29 1729.0 30 2007.0 31 2475.0 32 2827.0 33 3518.0 34 5094.0 35 6593.0 36 7569.0 37 7363.0 38 1789.0 39 15.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.29128131453713 21.62521325312023 19.21163688605549 10.871868546287152 2 14.039214278019177 31.24573562681797 31.807735124070813 22.90731497109204 3 29.6459223614752 27.103458182209934 34.37713218659101 8.873487269723848 4 10.751413950983032 22.17254690726277 40.93365652212945 26.142382619624744 5 7.796591908926039 44.057388087841844 35.54793413657503 12.59808586665709 6 20.88158721608762 39.66783373731933 16.25100996498788 23.19956908160517 7 16.777089505341593 41.22991291857438 24.467187359727085 17.525810216356945 8 21.468713529042105 25.109973965346978 22.052248855373012 31.369063650237905 9 15.87575186282431 28.85178202711195 29.392225513959964 25.880240596103782 10-11 23.348595026483526 26.41439985636054 25.943082862016336 24.2939222551396 12-13 32.26912786626205 27.059129841446556 25.38112082742274 15.29062146486865 14-15 22.267809848722898 31.78076042555102 34.36279570857835 11.588634017147731 16-17 14.135918843702308 33.527246611006376 33.749887781668015 18.586946763623306 18-19 13.452733638567196 38.056378489990124 32.567555435856 15.923332435586676 20-21 18.899362599874316 29.263847742167158 29.845587575186283 21.99120208277224 22-23 15.206169370404618 30.264209212758892 34.25742218711004 20.272199229726453 24-25 22.525563565522628 30.99139053227877 30.773236136422806 15.709809765775795 26-27 19.5216762876047 26.028602465234446 40.356767723922474 14.09295352323838 28-29 13.734626088517821 37.96480833108897 33.62510099649879 14.675464583894424 30-31 16.384774216716043 30.95430469521501 32.106113654726634 20.55480743334231 32-33 16.93868390340246 32.164467187359726 35.18358919113027 15.71325971810755 34-35 20.819642696830954 29.60768471137445 33.18969386839034 16.382978723404253 36-37 17.425262590896846 28.471137445013017 39.551126672053144 14.552473292036987 38-39 16.991380858322856 31.92314598671216 36.335966959956906 14.749506195008081 40-41 15.685711207670487 33.74420943010019 34.12575860954502 16.44432075268431 42-43 14.573121465122544 34.56234850525182 29.719903043361164 21.144626986264477 44-45 18.34635065984379 28.56180985725828 31.722775832660023 21.3690636502379 46-47 21.926564323547897 33.39617559924589 32.17703564054224 12.500224436663974 48-49 19.492773139420056 30.78732381721878 33.33602657330101 16.383876470060148 50-51 11.783136424031314 34.01712931375013 38.930584982224296 15.269149279994254 52-53 17.23509502733663 36.57452710770363 28.812539837865497 17.377838027094235 54-55 13.759762994882843 30.563784899901247 32.91229015171918 22.76416195349672 56-57 20.107729598707245 29.308735074961845 30.773857617380372 19.809677708950534 58-59 24.829202165384373 28.906804082988447 30.113386420562172 16.150607331065007 60-61 17.36901641110353 32.72165762918806 36.52996732143499 13.379358638273423 62-63 14.988778166801328 32.19050184038064 35.07675733907891 17.743962653739114 64-65 14.241852949097764 38.42625011221833 31.149115719543946 16.182781219139958 66-67 17.700870814256216 30.640991112308107 28.856270760391418 22.80186731304426 68-69 14.589280904928629 31.722775832660023 32.856629859053776 20.831313403357573 70-71 21.637489900350122 31.86013107101176 28.09587934284945 18.40649968578867 72-73 18.43253433880959 26.548164108088695 37.13169943441961 17.887602118682107 74-75 14.049215797175613 37.61300690385772 30.624758724088093 17.713018574878575 76-77 17.160427327408208 29.03671783822605 30.155310171469612 23.64754466289613 78-79 17.266361432803663 30.418349941646465 33.20854654816411 19.10674207738576 80-81 20.653559565490617 29.455965526528416 30.590717299578056 19.29975760840291 82-83 16.52302720172367 28.02585510368974 36.45390070921986 18.99721698536673 84-85 15.710707520491251 31.107200890572678 32.93233622709603 20.249755361840037 86-87 16.268067151449863 32.34222102522668 30.351018942454438 21.03869288086902 88-89 16.013250859600138 31.91877115334548 27.17323972744167 24.89473825961271 90-91 18.439716312056735 26.309363497620968 29.845587575186283 25.405332615136007 92-93 20.945327228656076 31.57195439446988 30.41206571505521 17.070652661818837 94-95 18.4163748990035 29.83481461531556 30.388724302001975 21.360086183678966 96-97 11.107819373372834 31.7829248586049 35.09561001885268 22.013645749169584 98-99 17.007810395906276 33.27857078732382 26.113654726636142 23.599964090133767 100-101 14.842845215330335 29.718010180721272 28.908220887536245 26.530923716412147 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3416.0 1 2134.0 2 739.0 3 537.0 4 382.5 5 355.0 6 509.5 7 843.0 8 1045.5 9 961.0 10 681.5 11 404.0 12 315.5 13 285.0 14 249.5 15 229.0 16 222.0 17 204.5 18 200.5 19 195.0 20 183.5 21 200.5 22 222.5 23 228.0 24 231.0 25 242.0 26 255.0 27 277.0 28 329.0 29 348.0 30 351.5 31 373.0 32 399.5 33 416.0 34 396.5 35 375.0 36 365.0 37 327.0 38 289.5 39 267.0 40 230.0 41 222.5 42 251.0 43 286.5 44 358.0 45 515.0 46 1095.0 47 4513.5 48 11515.5 49 10955.0 50 4123.5 51 1297.5 52 487.5 53 260.5 54 203.0 55 160.5 56 147.5 57 141.5 58 126.0 59 106.0 60 98.5 61 92.0 62 63.0 63 41.5 64 27.5 65 19.0 66 15.0 67 15.0 68 14.5 69 9.5 70 10.0 71 6.5 72 2.5 73 2.5 74 1.0 75 0.0 76 1.0 77 1.5 78 1.0 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017954933117874137 2 0.0017954933117874136 3 0.0017954933117874136 4 0.0 5 0.007181973247149654 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007181973247149654 14-15 0.004488733279468534 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.977466558937068E-4 24-25 8.977466558937068E-4 26-27 8.977466558937068E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.008977466558937068 40-41 0.0017954933117874136 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0017954933117874136 52-53 8.977466558937068E-4 54-55 0.0 56-57 0.0 58-59 8.977466558937068E-4 60-61 0.0017954933117874136 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0026932399676811206 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 8.977466558937068E-4 86-87 0.0 88-89 8.977466558937068E-4 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0026932399676811206 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 55695.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.68049196516743 #Duplication Level Percentage of deduplicated Percentage of total 1 82.94842854662268 17.15414310081695 2 8.603924292411877 3.558667743962654 3 2.6480291717312032 1.6428763802854833 4 1.3457197430109393 1.1132058533081963 5 0.7379753429414829 0.7630846575096508 6 0.7206112172252127 0.894155669270132 7 0.41673901719048445 0.603285752760571 8 0.2344156971696475 0.38782655534608135 9 0.14759506858829657 0.27471047670347426 >10 1.510678937315506 5.939491875392764 >50 0.19968744573710712 3.0954304695215007 >100 0.29519013717659315 13.49672322470599 >500 0.1128668171557562 17.570697549151628 >1k 0.07813856572321584 33.50570069126492 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3980 7.146063380913906 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3230 5.799443397073346 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3228 5.795852410449771 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2020 3.6268964898105756 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1546 2.7758326600233416 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1241 2.2282071999281805 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 1169 2.0989316814794865 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1129 2.02711194900799 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 1118 2.0073615225783286 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 962 1.727264565939492 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 918 1.6482628602208456 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 894 1.605171020737948 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 852 1.5297603016428765 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 822 1.475895502289254 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 806 1.4471676093006554 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 786 1.4112577430649071 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 714 1.2819822246162134 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 701 1.258640811562977 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 613 1.1006374001256845 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 613 1.1006374001256845 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 572 1.0270221743424006 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 533 0.9569979351826914 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 495 0.8887691893347698 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 468 0.8402908699165096 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 465 0.8349043899811474 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 425 0.7630846575096508 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 383 0.6876739384145794 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 320 0.5745578597719724 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 315 0.5655803932130353 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 285 0.5117155938594129 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 272 0.48837418080617645 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 237 0.425531914893617 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 226 0.4057814884639555 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 206 0.3698716222282072 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 205 0.36807612891641983 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 202 0.36268964898105754 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 196 0.3519166891103331 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 190 0.34114372923960856 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 185 0.3321662626806715 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 182 0.3267797827453093 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 178 0.31959780949815964 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 165 0.29625639644492324 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 163 0.2926654098213484 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 159 0.2854834365741988 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 155 0.2783014633270491 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 148 0.2657330101445372 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 146 0.2621420235209624 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 144 0.25855103689738757 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 143 0.25675554358560015 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 141 0.25316455696202533 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 135 0.24239159709130084 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 130 0.23341413053236376 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 122 0.21905018403806445 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 113 0.20289074423197775 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 112 0.20109525092019032 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 106 0.19032229104946585 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 97 0.17416285124337913 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 96 0.17236735793159172 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 96 0.17236735793159172 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 90 0.16159439806086723 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 89 0.15979890474907982 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 85 0.15261693150193018 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 82 0.14723045156656792 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 79 0.14184397163120568 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 78 0.14004847831941827 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 77 0.13825298500763086 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 74 0.1328665050722686 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 74 0.1328665050722686 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 73 0.1310710117604812 No Hit CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA 71 0.12748002513690634 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 69 0.12388903851333152 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 69 0.12388903851333152 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 68 0.12209354520154411 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 68 0.12209354520154411 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 67 0.1202980518897567 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 60 0.10772959870724481 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 57 0.10234311877188257 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0017954933117874136 0.0 24-25 0.0 0.0 0.0 0.01077295987072448 0.0 26-27 0.0 0.0 0.0 0.020648173085555256 0.0 28-29 0.0 0.0 0.0 0.05835353263309094 0.0 30-31 0.0 0.0 0.0 0.11491157195439447 0.0 32-33 0.0 0.0 0.0 0.15979890474907982 0.0 34-35 0.0 0.0 0.0 0.18762905108178474 0.0 36-37 0.0 0.0 0.0 0.23251638387647006 0.0 38-39 0.0 0.0 0.0 0.3438369692072897 0.0 40-41 0.0 0.0 0.0 0.4389981147320226 0.0 42-43 0.0 0.0 0.0 0.5224885537301374 0.0 44-45 0.0 0.0 0.0 0.5592961666217793 0.0 46-47 0.0 0.0 0.0 0.6203429392225515 0.0 48-49 0.0 0.0 0.0 0.6607415387377682 0.0 50-51 0.0 0.0 0.0 0.7137085914354969 0.0 52-53 0.0 0.0 0.0 0.8277224167339976 0.0 54-55 0.0 0.0 0.0 0.8833827093994076 0.0 56-57 0.0 0.0 0.0 1.0279199209982943 0.0 58-59 0.0 0.0 0.0 1.2074692521770356 0.0 60-61 0.0 0.0 0.0 1.359188437023072 0.0 62-63 0.0 0.0 0.0 1.448065355956549 0.0 64-65 0.0 0.0 0.0 1.613250740640991 0.0 66-67 0.0 0.0 0.0 1.9184846036448513 0.0 68-69 0.0 0.0 0.0 2.3467097585061496 0.0 70-71 0.0 0.0 0.0 2.687853487745758 0.0 72-73 0.0 0.0 0.0 3.0334859502648355 0.0 74-75 0.0 0.0 0.0 3.571236197145166 0.0 76-77 0.0 0.0 0.0 3.7920818744950173 0.0 78-79 0.0 0.0 0.0 4.241852949097765 0.0 80-81 0.0 0.0 0.0 4.713169943441961 0.0 82-83 0.0 0.0 0.0 4.99147140676901 0.0 84-85 0.0 0.0 0.0 5.485232067510548 0.0 86-87 0.0 0.0 0.0 6.177394739204596 0.0 88-89 0.0 0.0 0.0 6.994344196067869 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 135 0.0 52.816746 9 GGGCTTG 40 5.32438E-4 47.535072 7 GGCTTGA 45 9.5115195E-4 42.2534 8 GCTTGAT 50 0.0015968214 38.028057 9 ATGGGCT 50 0.0016039012 37.993893 5 CATGGGC 65 1.313252E-4 36.53259 4 TGGGCTT 55 0.002549411 34.57096 6 CAGAAAA 140 0.0 33.95362 10-11 GAGTACT 610 0.0 23.756853 12-13 GTACTTT 610 0.0 23.746181 14-15 ACTTTTT 650 0.0 22.284878 16-17 AGAAAAA 225 0.0 20.061344 12-13 GCAGAAA 180 3.6379788E-12 19.80628 10-11 AGTACAT 210 1.8899322E-5 18.108599 7 AGTACTT 605 0.0 18.063063 12-13 TACTTTT 630 0.0 17.33848 14-15 GTACATG 250 4.3740692E-6 17.112625 1 TACATGG 250 4.3740692E-6 17.112625 9 ACATGGG 250 4.411835E-6 17.09725 3 GCAGAGA 145 1.0106378E-6 16.376677 94-95 >>END_MODULE