FastQCFastQC Report
Thu 26 May 2016
SRR936767_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936767_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787832
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79960.447245602495089No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57210.31999650973917015No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38750.21674296018865308No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19560.10940625293651753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55200.035.8177641
GTATCAA85850.035.0988731
ACCGTAC650.00583006629.2460028
TCAACGC105500.028.3370464
ATCAACG105950.028.260763
TATCAAC106750.028.1833232
CAACGCA106150.028.161955
AACGCAG109200.027.331856
GTATTAC2700.026.404121
TACATGG70850.024.418442
ACGCAGA121550.024.1240437
CGCAGAG121450.024.0656438
GTACATG75350.024.031781
TATAGGG8600.023.2117142
GAGTACT64350.022.62977412-13
ACATGGG73650.022.1342893
AGAGTAC95750.021.93371610-11
GCAGAGT132450.021.1340739
AGTACTT66750.021.06874812-13
GTACTTT69400.020.64164714-15
Produced by FastQC (version 0.11.5)