##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936767_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1787832 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87078539818059 33.0 31.0 34.0 30.0 34.0 2 31.974107746141694 33.0 31.0 34.0 30.0 34.0 3 31.99064509416992 34.0 31.0 34.0 30.0 34.0 4 35.566991193803446 37.0 35.0 37.0 33.0 37.0 5 35.57745917960972 37.0 35.0 37.0 33.0 37.0 6 35.6317249048009 37.0 35.0 37.0 33.0 37.0 7 35.5683559752818 37.0 35.0 37.0 33.0 37.0 8 35.52993961401295 37.0 35.0 37.0 33.0 37.0 9 37.174784319779484 39.0 37.0 39.0 34.0 39.0 10-11 37.05713735966243 39.0 37.0 39.0 33.0 39.0 12-13 36.95777343732521 39.0 37.0 39.0 33.0 39.0 14-15 38.155388481691794 40.0 38.0 41.0 33.0 41.0 16-17 38.08213243749972 40.0 38.0 41.0 33.0 41.0 18-19 38.02753978002407 40.0 38.0 41.0 33.0 41.0 20-21 37.961062616621696 40.0 38.0 41.0 33.0 41.0 22-23 37.828227708196295 40.0 38.0 41.0 33.0 41.0 24-25 37.664947545406946 40.0 37.0 41.0 32.0 41.0 26-27 37.609428346735044 40.0 37.0 41.0 32.0 41.0 28-29 37.68475841130487 40.0 37.0 41.0 33.0 41.0 30-31 37.54092946093369 40.0 37.0 41.0 32.0 41.0 32-33 37.34750524657798 40.0 37.0 41.0 32.0 41.0 34-35 37.14892646512648 40.0 36.0 41.0 31.0 41.0 36-37 37.00712007615928 39.0 36.0 41.0 31.0 41.0 38-39 36.83258354252525 39.0 36.0 41.0 31.0 41.0 40-41 36.61914570272822 39.0 35.5 41.0 30.0 41.0 42-43 36.40496394515816 39.0 35.0 41.0 30.0 41.0 44-45 36.13845791998354 39.0 35.0 40.5 29.5 41.0 46-47 35.78060886034035 39.0 35.0 40.0 28.5 41.0 48-49 35.41775150014095 38.5 35.0 40.0 27.0 41.0 50-51 34.74554292573352 37.5 34.0 39.5 26.0 40.5 52-53 35.0714331659798 38.5 34.5 40.0 27.0 40.5 54-55 35.383136390891316 39.0 35.0 40.0 27.5 41.0 56-57 35.233196407716164 38.5 35.0 40.0 27.0 41.0 58-59 34.96582816506249 38.0 34.5 40.0 26.0 41.0 60-61 34.641062191525826 38.0 34.0 40.0 26.0 41.0 62-63 34.3181344779599 37.0 34.0 40.0 26.0 41.0 64-65 33.96456350484833 37.0 34.0 39.5 25.5 41.0 66-67 33.557695297992204 36.0 33.5 39.0 24.5 41.0 68-69 33.105518303733234 35.5 33.0 39.0 23.0 40.5 70-71 32.72181838114543 35.0 33.0 38.5 22.5 40.0 72-73 32.33431049449837 35.0 32.0 37.0 22.0 39.5 74-75 31.914964605175435 35.0 32.0 37.0 20.5 39.0 76-77 31.502369629808616 35.0 31.5 36.0 19.5 39.0 78-79 31.10350357304266 35.0 31.0 36.0 18.0 37.5 80-81 30.726058992120066 35.0 31.0 35.0 16.5 37.0 82-83 30.35206663713369 34.0 31.0 35.0 12.0 37.0 84-85 30.03830617194457 34.0 30.0 35.0 7.5 36.0 86-87 29.75295245862027 34.0 30.0 35.0 7.0 36.0 88-89 29.40739034763893 34.0 30.0 35.0 2.0 35.5 90-91 29.087469907687076 34.0 29.0 35.0 2.0 35.0 92-93 28.73485204426367 34.0 29.0 35.0 2.0 35.0 94-95 28.346846627647338 34.0 29.0 35.0 2.0 35.0 96-97 27.911230193888464 34.0 29.0 35.0 2.0 35.0 98-99 27.341208513999078 34.0 27.0 35.0 2.0 35.0 100-101 25.25099450060185 32.0 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34027548156557685 1101 2 0.27718403160008975 1101 3 0.46655945283016464 1101 4 0.23862147401781897 1101 5 0.2689740214277805 1101 6 0.19121281609363905 1101 7 0.24749248685116498 1101 8 0.22970493054069152 1101 9 0.33170278155836996 1101 10-11 0.34436814436651986 1101 12-13 0.46426034750456324 1101 14-15 0.6575683311431888 1101 16-17 0.5985417708906979 1101 18-19 0.6198719355061826 1101 20-21 0.5804544031380487 1101 22-23 0.568016730607269 1101 24-25 0.6612987312176273 1101 26-27 0.6448905943210264 1101 28-29 0.7259794391249912 1101 30-31 0.6261406370531333 1101 32-33 0.6441221805141666 1101 34-35 0.7107664459473142 1101 36-37 0.6983310224843535 1101 38-39 0.8027644465262682 1101 40-41 0.7857794958952766 1101 42-43 0.8507070876030696 1101 44-45 0.765077225719125 1101 46-47 0.8285915539098809 1101 48-49 0.8522855878208091 1101 50-51 0.9056691186827521 1101 52-53 0.9456709168268489 1101 54-55 0.9493567352916656 1101 56-57 0.7903896001949633 1101 58-59 0.7395852555929068 1101 60-61 0.8154496812404091 1101 62-63 0.7454504428530839 1101 64-65 0.7414265778310138 1101 66-67 0.6585551764079582 1101 68-69 0.643970465482326 1101 70-71 0.6518230866160977 1101 72-73 0.723291642366604 1101 74-75 0.7621022238449626 1101 76-77 0.6813259689729598 1101 78-79 0.7958698933100052 1101 80-81 0.7454019516463966 1101 82-83 0.5798186727222152 1101 84-85 0.7727770964152292 1101 86-87 0.7198182046867139 1101 88-89 0.8984946476295868 1101 90-91 0.9968416449710773 1101 92-93 0.773950074811804 1101 94-95 0.8724069464633324 1101 96-97 0.9009841331847142 1101 98-99 1.0151212991829937 1101 100-101 1.0232043691400534 1102 1 0.3591675164116239 1102 2 0.28832071233638956 1102 3 0.2621122945948109 1102 4 0.24913419093488898 1102 5 0.2755215304943235 1102 6 0.2149741439946169 1102 7 0.2203730139955269 1102 8 0.23645388034243808 1102 9 0.2796820820382919 1102 10-11 0.40164583041998725 1102 12-13 0.3377069185470276 1102 14-15 0.46456007184465875 1102 16-17 0.37538272109715365 1102 18-19 0.36150849074170566 1102 20-21 0.30173259713278355 1102 22-23 0.34497387466183227 1102 24-25 0.4042820502346629 1102 26-27 0.3734729562890209 1102 28-29 0.4895919909383153 1102 30-31 0.3995797625536852 1102 32-33 0.44007310754123097 1102 34-35 0.2895097321126059 1102 36-37 0.3332104401059368 1102 38-39 0.21538010651075723 1102 40-41 0.31193852810272205 1102 42-43 0.2540998680310622 1102 44-45 0.30944648896904425 1102 46-47 0.1839639577624652 1102 48-49 0.24878319120136183 1102 50-51 0.06714887674374381 1102 52-53 0.3656499551140655 1102 54-55 0.4339680448871164 1102 56-57 0.47943367940182924 1102 58-59 0.3570078465090276 1102 60-61 0.4900097262242724 1102 62-63 0.5053864157604551 1102 64-65 0.6098473509843103 1102 66-67 0.35543865755965953 1102 68-69 0.27471229725465207 1102 70-71 0.41837422938116475 1102 72-73 0.31922750701670566 1102 74-75 0.354001622697389 1102 76-77 0.34494854714487033 1102 78-79 0.4199090244289678 1102 80-81 0.42081355193773007 1102 82-83 0.44262405699097584 1102 84-85 0.4407658796835001 1102 86-87 0.48503954169135355 1102 88-89 0.3832105031394839 1102 90-91 0.3247783117298617 1102 92-93 0.35038592815655534 1102 94-95 0.47596998085511544 1102 96-97 0.3054018746777807 1102 98-99 0.3564223139920841 1102 100-101 0.5402799544087493 1103 1 0.31997724224383717 1103 2 0.41921686977178396 1103 3 0.35172544659265625 1103 4 0.31332481681184987 1103 5 0.22673663014649037 1103 6 0.19838951400624438 1103 7 0.24989103915223865 1103 8 0.2918953395233217 1103 9 0.36544642504679103 1103 10-11 0.45547238917652777 1103 12-13 0.2768653044166598 1103 14-15 0.38077498762080353 1103 16-17 0.5426224680343807 1103 18-19 0.656675476085681 1103 20-21 0.5054341572159444 1103 22-23 0.3940361933310754 1103 24-25 0.6780788778426228 1103 26-27 0.6540351874628954 1103 28-29 0.6231407142587031 1103 30-31 0.7466705600400374 1103 32-33 0.6933626989489667 1103 34-35 0.6735725061526665 1103 36-37 0.8293903848839008 1103 38-39 0.7368586153527232 1103 40-41 0.8933471689923635 1103 42-43 0.8450015713647474 1103 44-45 0.7666940796378157 1103 46-47 0.8194947782222144 1103 48-49 0.9933128795277995 1103 50-51 0.9261355890478669 1103 52-53 1.0336298338941035 1103 54-55 0.9224726466005464 1103 56-57 1.0421597759508003 1103 58-59 1.0876125666188656 1103 60-61 0.9597634779738655 1103 62-63 1.013686381971695 1103 64-65 1.0024762795448936 1103 66-67 0.8735390306675583 1103 68-69 0.9080872639039796 1103 70-71 0.933412634788553 1103 72-73 0.8288001050960503 1103 74-75 0.8921827383583718 1103 76-77 0.7900263217661809 1103 78-79 0.7831685095316772 1103 80-81 0.8317052568584238 1103 82-83 0.8624141273402124 1103 84-85 1.059434034799832 1103 86-87 1.02897844499552 1103 88-89 1.088002238245462 1103 90-91 0.9968173462647414 1103 92-93 0.9730551144594912 1103 94-95 0.9971520050364155 1103 96-97 0.9026300855542075 1103 98-99 1.279974438401858 1103 100-101 1.6041062219254059 1104 1 0.3607984942584608 1104 2 0.36644978181989885 1104 3 0.38313082372814833 1104 4 0.37453939461605756 1104 5 0.27420899714234537 1104 6 0.30634657472345594 1104 7 0.3093744560748135 1104 8 0.3068452906341861 1104 9 0.42022816962922604 1104 10-11 0.5154359405367401 1104 12-13 0.4476266407224969 1104 14-15 0.5543845235577649 1104 16-17 0.5469829060388705 1104 18-19 0.4743387278459963 1104 20-21 0.4961531243630404 1104 22-23 0.4986429921315363 1104 24-25 0.486120930329065 1104 26-27 0.577970828944288 1104 28-29 0.5335204604452883 1104 30-31 0.5369111868461474 1104 32-33 0.6253854446771996 1104 34-35 0.535316427902714 1104 36-37 0.6625836790062536 1104 38-39 0.6301565679609453 1104 40-41 0.6849097317768482 1104 42-43 0.7956696801433907 1104 44-45 0.6633064834240443 1104 46-47 0.7383110503371242 1104 48-49 0.6596958368626176 1104 50-51 0.8603701284555569 1104 52-53 0.8825665397304547 1104 54-55 0.7880172766770315 1104 56-57 0.7681233304435722 1104 58-59 0.8099100412284841 1104 60-61 0.8241742674433254 1104 62-63 0.7225034984511325 1104 64-65 0.7995687892910368 1104 66-67 0.7158773765075992 1104 68-69 0.7605635373317199 1104 70-71 0.6846844143743809 1104 72-73 0.7479601033475092 1104 74-75 0.7637514039192439 1104 76-77 0.7015526523415865 1104 78-79 0.7297577538083324 1104 80-81 0.8242401773123085 1104 82-83 0.8156655217164008 1104 84-85 0.825730448329427 1104 86-87 0.9167091448227858 1104 88-89 0.9039385295885332 1104 90-91 0.9425502653610884 1104 92-93 0.8775902348270037 1104 94-95 0.6687141349594832 1104 96-97 0.5854822094157939 1104 98-99 0.5518853065410241 1104 100-101 0.7244584258805951 1105 1 0.17118100166171857 1105 2 0.25777349990014287 1105 3 0.1804654905869576 1105 4 0.17011347065182036 1105 5 0.18247710956439533 1105 6 0.1670718955155408 1105 7 0.28130918458371923 1105 8 0.19872236452555825 1105 9 0.11675378225725552 1105 10-11 0.2356275743821996 1105 12-13 0.20532017926351642 1105 14-15 0.29021223664747 1105 16-17 0.35180958749138114 1105 18-19 0.27506470712990705 1105 20-21 0.4161547181041243 1105 22-23 0.2773135512165368 1105 24-25 0.45596351579064986 1105 26-27 0.2655577331642647 1105 28-29 0.17975932692318253 1105 30-31 0.06897043393231428 1105 32-33 0.2583530764638411 1105 34-35 0.24527559186622483 1105 36-37 0.300109378478858 1105 38-39 0.3517565631256474 1105 40-41 0.5179931881399895 1105 42-43 0.38432701531286284 1105 44-45 0.2946515054644365 1105 46-47 0.29868425296460543 1105 48-49 0.3511307032730784 1105 50-51 0.3083333687061369 1105 52-53 0.28554723319552267 1105 54-55 0.3676017397055844 1105 56-57 0.4175245482399035 1105 58-59 0.5193429684282336 1105 60-61 0.3308108788889257 1105 62-63 0.3103414566556708 1105 64-65 0.3547173205520622 1105 66-67 0.4473972169935223 1105 68-69 0.5103300431291444 1105 70-71 0.4151534514207569 1105 72-73 0.45941654093219597 1105 74-75 0.49493698822894316 1105 76-77 0.29867033501023954 1105 78-79 0.47634942239162115 1105 80-81 0.5898551816370379 1105 82-83 0.5887702343228973 1105 84-85 0.7138737622356608 1105 86-87 0.7879669151058479 1105 88-89 0.7547956982581887 1105 90-91 0.7711361748448695 1105 92-93 0.833893019547574 1105 94-95 0.8939997756704408 1105 96-97 0.8138012070321778 1105 98-99 0.8890510941051986 1105 100-101 0.9421077086811991 1106 1 0.44093484663680016 1106 2 0.4949134494294718 1106 3 0.426632794276383 1106 4 0.33863717899853896 1106 5 0.3777312163229283 1106 6 0.3786832929676507 1106 7 0.39422969573432454 1106 8 0.2989500620983847 1106 9 0.4297028783921917 1106 10-11 0.643941973847042 1106 12-13 0.539498292202687 1106 14-15 0.6776632811781624 1106 16-17 0.7045279114486505 1106 18-19 0.6385188537698951 1106 20-21 0.6932731257535067 1106 22-23 0.8000391049155482 1106 24-25 0.6404646095837734 1106 26-27 0.6809803145160487 1106 28-29 0.6679142398568629 1106 30-31 0.6275998576059933 1106 32-33 0.586537183409007 1106 34-35 0.512607326012116 1106 36-37 0.48370700012957 1106 38-39 0.5458542550109442 1106 40-41 0.5420102232189734 1106 42-43 0.49857314283333665 1106 44-45 0.7004851015635154 1106 46-47 0.42791711449834935 1106 48-49 0.46755434845778154 1106 50-51 0.47058286945122063 1106 52-53 0.44793572965480877 1106 54-55 0.49761995158220174 1106 56-57 0.5938123705454217 1106 58-59 0.806160133563985 1106 60-61 0.7001470491100505 1106 62-63 0.522931717918496 1106 64-65 0.4682505424923633 1106 66-67 0.5680221672644876 1106 68-69 0.39528972454900213 1106 70-71 0.38028468203871313 1106 72-73 0.5158492920552078 1106 74-75 0.6521232139992463 1106 76-77 0.7126437755323529 1106 78-79 0.8089305580701023 1106 80-81 0.8842061109437225 1106 82-83 0.8142392097918716 1106 84-85 0.6995773408595944 1106 86-87 0.7789434901532175 1106 88-89 0.6541905511928654 1106 90-91 0.6793582379341885 1106 92-93 0.8308022067222502 1106 94-95 0.7398026944822078 1106 96-97 0.6177963760715954 1106 98-99 0.8158627288814344 1106 100-101 1.018482906737816 1107 1 0.09737033142252827 1107 2 0.22455555200270183 1107 3 0.25484678826720497 1107 4 0.24673711726398295 1107 5 0.14500662348088156 1107 6 0.12274142383837727 1107 7 0.1830841645140424 1107 8 0.20304964051724284 1107 9 0.23027191841664774 1107 10-11 0.3311282081502043 1107 12-13 0.16132119552585777 1107 14-15 0.13586191649906 1107 16-17 0.3030914911921556 1107 18-19 0.14329089551470275 1107 20-21 0.3216888163688765 1107 22-23 0.2802755736731086 1107 24-25 0.2918834286480845 1107 26-27 0.3475759245179617 1107 28-29 0.24309822120742552 1107 30-31 0.228665903146144 1107 32-33 0.328985457090603 1107 34-35 0.3824248342439347 1107 36-37 0.3008348506263516 1107 38-39 0.35628077753036536 1107 40-41 0.5367495854169633 1107 42-43 0.4910942658936932 1107 44-45 0.5548070417103332 1107 46-47 0.5579522777689618 1107 48-49 0.4906850855500764 1107 50-51 0.47566358395530983 1107 52-53 0.4564048366289839 1107 54-55 0.5598621674549591 1107 56-57 0.5999364446086304 1107 58-59 0.611534950951615 1107 60-61 0.5664617571225676 1107 62-63 0.521843540508911 1107 64-65 0.6773932862053229 1107 66-67 0.7538746223374488 1107 68-69 0.7443876960441997 1107 70-71 0.6608586047642646 1107 72-73 0.6578614147133521 1107 74-75 0.5928919428085493 1107 76-77 0.6900187467674463 1107 78-79 0.7442183082270475 1107 80-81 0.8134776758266078 1107 82-83 0.8738986136851246 1107 84-85 0.7801665179517094 1107 86-87 0.7619842525586371 1107 88-89 0.5895751079068425 1107 90-91 0.6004757245882466 1107 92-93 0.5982955101205185 1107 94-95 0.7510832659603217 1107 96-97 0.8160074756441489 1107 98-99 0.6807093670863225 1107 100-101 0.8799598944828233 1108 1 0.1488756160343243 1108 2 0.24507232781293098 1108 3 0.13845705162416166 1108 4 0.1460163619467636 1108 5 0.22963710844126695 1108 6 0.08620162188203295 1108 7 0.13044247974680445 1108 8 0.13905448642132257 1108 9 0.17320831916622126 1108 10-11 0.14176445990928954 1108 12-13 0.03260537901235239 1108 14-15 0.2841696983826054 1108 16-17 0.3672958044896859 1108 18-19 0.11094859061541484 1108 20-21 0.27240475116737173 1108 22-23 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2316 76-77 -2.061440634573156 2316 78-79 -2.141865255009055 2316 80-81 -2.000100112850067 2316 82-83 -2.0583490367602018 2316 84-85 -1.988232107499968 2316 86-87 -1.8983471293001735 2316 88-89 -2.1521581490895194 2316 90-91 -1.9807123077806672 2316 92-93 -2.241655750626439 2316 94-95 -2.246661628266967 2316 96-97 -1.9861352570612425 2316 98-99 -2.196552930482227 2316 100-101 -2.0740468108861023 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5044.0 3 1500.0 4 1482.0 5 1516.0 6 1688.0 7 1926.0 8 2280.0 9 2761.0 10 3393.0 11 4285.0 12 5393.0 13 6624.0 14 7320.0 15 9191.0 16 11982.0 17 16210.0 18 14156.0 19 9604.0 20 9383.0 21 9757.0 22 10370.0 23 11431.0 24 13110.0 25 14874.0 26 17546.0 27 20923.0 28 25216.0 29 30936.0 30 38506.0 31 48951.0 32 63674.0 33 85083.0 34 118122.0 35 174699.0 36 269370.0 37 381931.0 38 291262.0 39 46303.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.648937432421235 17.59683770575844 14.02862317299491 23.725601688825414 2 15.84373486657392 19.7267935471771 39.437183510199056 24.992288076049928 3 19.38054568443394 23.87743005434232 29.170335104444835 27.571689156778913 4 13.368310398302821 15.86832299945507 35.86539953213322 34.89796707010889 5 14.646930402905792 36.181283809018275 33.573933439383275 15.597852348692657 6 33.198527229787764 34.45607052992628 17.194357068389976 15.15104517189598 7 29.235632981987752 31.81232277803411 20.60881484700117 18.34322939297697 8 26.99084374800543 33.32620421454094 19.902110853691447 19.78084118376218 9 27.02826654481556 16.06967464186638 19.607724553781917 37.294334259536136 10-11 25.427097917907275 25.64555681261109 26.950150888659874 21.977194380821754 12-13 26.48669862266726 23.020105183836577 27.338811984731887 23.154384208764274 14-15 23.55222114946686 24.088119839308774 25.714431278075267 26.645227733149103 16-17 22.88308839215673 27.212827537782648 26.19193600677291 23.712148063287717 18-19 22.9030288797559 25.954105100400515 28.46017833865759 22.682687681185993 20-21 23.794295456593517 25.05839267753305 28.470663171781656 22.676648694091774 22-23 23.503869850985197 24.82805715429168 28.57991651588871 23.088156478834414 24-25 23.344766571922925 25.01299146858583 28.61181715735754 23.030424802133705 26-27 23.042889317250108 25.26137946800447 28.533613966204857 23.16211724854057 28-29 22.978607057622476 25.520439209610764 28.588475505187322 22.912478227579445 30-31 23.14216356145894 25.094683933613805 28.355711646011915 23.407440858915344 32-33 23.04401839975825 24.985030610303415 28.63882080381426 23.332130186124076 34-35 23.503031205129265 25.09610660530766 28.193705031341665 23.20715715822141 36-37 23.314438310253873 25.115607154758717 28.291722592073715 23.278231942913692 38-39 23.097748913461054 25.265230781628123 28.47153146241301 23.165488842497812 40-41 23.345185352672665 25.118678767554158 28.271879316481936 23.264256563291237 42-43 23.14002155215232 25.219320152997337 27.972186474527355 23.668471820322992 44-45 23.124576287073044 25.359766025127172 27.904911474600723 23.610746213199054 46-47 23.5350760651201 25.580311444695948 27.62565872794064 23.258953762243312 48-49 23.560883678273605 25.67556981312401 27.32523427344875 23.438312235153635 50-51 23.404511247908275 25.79396094548559 27.377938557647173 23.42358924895896 52-53 23.52645111111733 25.881353988020134 26.889626867133142 23.7025680337294 54-55 23.403228432860917 25.87438355420834 26.802136918814657 23.92025109411609 56-57 23.425132463663253 26.11729885766616 26.816543597242195 23.641025081428392 58-59 23.568989180938416 25.73968495586612 27.06349150830282 23.62783435489264 60-61 23.43545304722279 25.606576550612637 27.374780002854017 23.583190399310556 62-63 23.14755015809945 25.711615412597588 27.578727929037132 23.56210650026583 64-65 23.341916812988696 25.835490394224557 27.169175124247825 23.653417668538918 66-67 23.209069870914124 26.03054631337115 26.90434549010939 23.856038325605343 68-69 23.055171365966768 25.838684395566634 27.317236823291974 23.788907415174624 70-71 23.460305800268937 25.5557697079319 26.99170335152821 23.992221140270956 72-73 23.1819665605875 25.953320878332132 26.962746604688164 23.901965956392203 74-75 23.100544328138838 26.225493624327985 26.648804005503145 24.025158042030032 76-77 23.45801669601166 25.996552464126406 26.67923283513521 23.866198004726723 78-79 23.206062907534896 26.079406917707647 26.770668373319246 23.94386180143821 80-81 23.29423146760135 25.805636644737618 26.766228701149945 24.133903186511088 82-83 23.36872769196974 25.87517437166735 26.809596633035266 23.946501303327647 84-85 23.151819245213325 26.0376497848024 26.602956238491547 24.207574731492727 86-87 23.30272954659046 25.981736233193242 26.73647665789634 23.979057562319955 88-89 23.36320678402945 25.80522002582974 26.518029035225073 24.31354415491574 90-91 23.125994310828034 25.933445363100976 26.709193203776177 24.231367122294813 92-93 23.179354708706548 26.000786428648393 26.56712700262936 24.2527318600157 94-95 23.2447435718382 25.865911577422445 26.63707548270864 24.252269368030717 96-97 23.098265662959356 26.050381120837603 26.59801051871678 24.253342697486264 98-99 23.01000179203441 26.060380359302897 26.41808722727476 24.51153062138793 100-101 23.08689519075749 25.78427875362103 26.496752903123564 24.632073152497917 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 516.0 1 373.5 2 271.0 3 349.5 4 361.0 5 370.5 6 582.0 7 1170.0 8 2013.0 9 2406.5 10 2156.5 11 2007.5 12 2102.5 13 2190.5 14 2308.5 15 2385.0 16 2482.5 17 2555.5 18 2642.0 19 2845.5 20 3053.5 21 3363.5 22 3921.0 23 4699.0 24 5499.5 25 6563.0 26 8278.0 27 10261.5 28 11716.5 29 13203.0 30 15125.5 31 17411.0 32 19959.0 33 21975.0 34 24108.0 35 26399.0 36 29740.0 37 33061.5 38 34800.5 39 35930.5 40 37571.0 41 40374.0 42 43067.0 43 46473.5 44 51186.5 45 55763.0 46 60503.0 47 66971.0 48 73448.5 49 75935.0 50 73861.0 51 72464.0 52 74725.0 53 77088.0 54 78316.0 55 74735.5 56 67651.0 57 61291.5 58 57014.0 59 51481.0 60 43564.0 61 36816.0 62 29158.0 63 22130.5 64 15730.5 65 10471.5 66 7469.5 67 5860.0 68 4646.5 69 3752.0 70 2894.0 71 1702.0 72 1067.5 73 713.0 74 412.0 75 196.5 76 118.0 77 67.5 78 40.0 79 29.5 80 24.5 81 20.5 82 20.0 83 19.0 84 13.5 85 9.0 86 9.0 87 11.0 88 10.5 89 8.5 90 8.0 91 7.0 92 5.0 93 2.5 94 2.0 95 0.5 96 1.5 97 1.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.057499809825531704 2 0.09156341311711615 3 0.06684073223882334 4 0.12758469475879164 5 0.1052112279006081 6 0.10123993753328053 7 0.10828757959360834 8 0.09676524416164382 9 0.11444028297960883 10-11 0.10697313841569007 12-13 0.0909481427785161 14-15 0.09360499196792539 16-17 0.10588243190635362 18-19 0.08454373789036106 20-21 0.06829500758460526 22-23 0.07072812210543272 24-25 0.060548194684959214 26-27 0.051375073273103956 28-29 0.06533052322589594 30-31 0.046648678399312686 32-33 0.047655484407930944 34-35 0.04217398502767598 36-37 0.04773938490864913 38-39 0.04757158390721276 40-41 0.06018462584851373 42-43 0.05612943498046796 44-45 0.04488676788423073 46-47 0.03246949377793886 48-49 0.03976883734042125 50-51 0.024219277873983685 52-53 0.037307755986021054 54-55 0.0432367247034397 56-57 0.0465647778985945 58-59 0.0525496802831586 60-61 0.04905382608656742 62-63 0.0549268611368406 64-65 0.037923026324621106 66-67 0.05800321282984083 68-69 0.04930552758872198 70-71 0.023687908036101823 72-73 0.018486076991574153 74-75 0.0274634305684203 76-77 0.042425686529830545 78-79 0.06144313335928656 80-81 0.0394332353375485 82-83 0.05926172034061366 84-85 0.06163890119429566 86-87 0.06292537553864122 88-89 0.084963240393952 90-91 0.06177873536215931 92-93 0.07128745877688732 94-95 0.07699269282572412 96-97 0.08879469659341593 98-99 0.12036925169702746 100-101 0.1165098286639908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1787832.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.91249246548486 #Duplication Level Percentage of deduplicated Percentage of total 1 71.78676712272276 34.39482938888971 2 14.345028618787875 13.746121512296778 3 5.339913097270707 7.6754563811798 4 2.6703813179054188 5.117784990964597 5 1.4624322592921353 3.503438730230822 6 0.9406761829107655 2.7042084315703865 7 0.6441771281503748 2.160489225925976 8 0.45368059574315667 1.7389574500224543 9 0.3323226749136522 1.4330166892119123 >10 1.8342773051513606 15.85590975638188 >50 0.12353170426405219 4.080183569681011 >100 0.06306811499362569 5.3090301973043745 >500 0.0025739160521746555 0.7695899125571734 >1k 9.359694735180566E-4 0.7420665206811757 >5k 2.3399236837951415E-4 0.7689172431019554 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7996 0.447245602495089 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5721 0.31999650973917015 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3875 0.21674296018865308 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1956 0.10940625293651753 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.593366714545886E-5 2 0.0 0.0 0.0 0.0 5.593366714545886E-5 3 0.0 0.0 0.0 0.0 5.593366714545886E-5 4 0.0 0.0 0.0 0.0 5.593366714545886E-5 5 0.0 0.0 0.0 0.0 5.593366714545886E-5 6 0.0 0.0 0.0 0.0 1.1186733429091772E-4 7 0.0 0.0 0.0 0.0 1.1186733429091772E-4 8 0.0 0.0 0.0 0.0 1.1186733429091772E-4 9 0.0 0.0 0.0 0.0 1.1186733429091772E-4 10-11 0.0 0.0 0.0 0.0 1.1186733429091772E-4 12-13 0.0 0.0 0.0 0.0 1.6780100143637657E-4 14-15 0.0 0.0 0.0 0.0 2.237346685818354E-4 16-17 0.0 0.0 0.0 0.0 2.796683357272943E-4 18-19 0.0 0.0 0.0 5.593366714545886E-5 3.91535670018212E-4 20-21 0.0 0.0 0.0 5.593366714545886E-5 3.91535670018212E-4 22-23 0.0 0.0 0.0 1.1186733429091772E-4 5.593366714545886E-4 24-25 0.0 0.0 0.0 3.076351693000237E-4 5.593366714545886E-4 26-27 0.0 0.0 0.0 6.991708393182357E-4 6.991708393182357E-4 28-29 0.0 0.0 0.0 0.0016220763472183067 7.271376728909652E-4 30-31 0.0 0.0 0.0 0.003887389866609391 7.551045064636946E-4 32-33 0.0 0.0 0.0 0.007187476228191464 8.390050071818829E-4 34-35 0.0 0.0 0.0 0.01029179475476443 8.949386743273418E-4 36-37 0.0 0.0 0.0 0.014654620792110221 8.949386743273418E-4 38-39 0.0 0.0 0.0 0.022792969361774485 8.949386743273418E-4 40-41 0.0 0.0 0.0 0.033476299786557126 8.949386743273418E-4 42-43 0.0 0.0 0.0 0.049081792920140144 8.949386743273418E-4 44-45 0.0 0.0 0.0 0.06261774036934119 9.229055079000712E-4 46-47 0.0 0.0 0.0 0.08971760210131602 9.508723414728006E-4 48-49 0.0 0.0 0.0 0.12134809087207299 9.508723414728006E-4 50-51 0.0 0.0 0.0 0.14156811154515636 9.508723414728006E-4 52-53 0.0 0.0 0.0 0.17345030181806792 9.508723414728006E-4 54-55 0.0 0.0 0.0 0.21903624054161688 9.508723414728006E-4 56-57 0.0 0.0 0.0 0.2897363958134769 9.508723414728006E-4 58-59 0.0 0.0 0.0 0.3703088433365104 9.508723414728006E-4 60-61 0.0 0.0 0.0 0.4613408866157447 9.508723414728006E-4 62-63 0.0 0.0 0.0 0.5641189999955253 9.508723414728006E-4 64-65 0.0 0.0 0.0 0.6757066659507157 0.0010347728421909888 66-67 0.0 0.0 0.0 0.8042422330509802 0.0010627396757637182 68-69 0.0 0.0 0.0 0.9663380004385199 0.0010627396757637182 70-71 0.0 0.0 0.0 1.1015856075962396 0.0010907065093364477 72-73 0.0 0.0 0.0 1.253361613395442 0.0012305406772000948 74-75 0.0 0.0 0.0 1.422057553506146 0.0012864743443455537 76-77 0.0 0.0 0.0 1.6184965925209975 0.0012864743443455537 78-79 0.0 0.0 0.0 1.8305131578358593 0.0013144411779182831 80-81 0.0 0.0 0.0 2.1046440605157533 0.0013424080114910126 82-83 0.0 0.0 0.0 2.392814313649157 0.0015381758465001186 84-85 0.0 0.0 0.0 2.7417285293025295 0.001566142680072848 86-87 0.0 0.0 0.0 3.1199799533736954 0.0015941095136455775 88-89 0.0 0.0 0.0 3.5503335883908553 0.001622076347218307 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5520 0.0 35.817764 1 GTATCAA 8585 0.0 35.098873 1 ACCGTAC 65 0.005830066 29.246002 8 TCAACGC 10550 0.0 28.337046 4 ATCAACG 10595 0.0 28.26076 3 TATCAAC 10675 0.0 28.183323 2 CAACGCA 10615 0.0 28.16195 5 AACGCAG 10920 0.0 27.33185 6 GTATTAC 270 0.0 26.40412 1 TACATGG 7085 0.0 24.41844 2 ACGCAGA 12155 0.0 24.124043 7 CGCAGAG 12145 0.0 24.065643 8 GTACATG 7535 0.0 24.03178 1 TATAGGG 860 0.0 23.211714 2 GAGTACT 6435 0.0 22.629774 12-13 ACATGGG 7365 0.0 22.134289 3 AGAGTAC 9575 0.0 21.933716 10-11 GCAGAGT 13245 0.0 21.134073 9 AGTACTT 6675 0.0 21.068748 12-13 GTACTTT 6940 0.0 20.641647 14-15 >>END_MODULE