FastQCFastQC Report
Thu 26 May 2016
SRR936767_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936767_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1787832
Sequences flagged as poor quality0
Sequence length101
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84230.47112927836619994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65410.3658621167984464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45650.25533719051901965No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28070.15700580367730302No Hit
CTATAGGGAAGGAGCACAAGTAAGGAACAGTCTGCATGATCCTTCTAGAC20270.11337754330384511No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA102500.029.7607421
GGTATCA72200.027.3771841
GTATAGG3450.024.7905431
TCAACGC127500.023.9166054
TATCAAC127650.023.889172
ATCAACG127650.023.889173
CAACGCA129150.023.611055
TATAGGG8800.023.2098642
AACGCAG131800.023.2077476
GGACCGT1652.4326491E-623.0286566
TATAGCG1452.3360697E-522.9300335
TACATGG71150.022.5646512
AGTACTT75700.021.74051912-13
CTATAGG9900.021.597821
GTACATG74250.021.5658251
AGAGTAC109700.021.47521810-11
GTCTAGA9700.021.0634421
GAGTACT76200.021.00571412-13
ACATGGG71600.020.963363
ACGCAGA146400.020.6986567
Produced by FastQC (version 0.11.5)