FastQCFastQC Report
Thu 26 May 2016
SRR936766_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936766_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294562
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62060.47938994038137994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43550.3364072172673074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29260.2260223921295388No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14460.111698010601269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63700.038.0473631
GGTATCA38600.032.1326871
TATCAAC77250.031.1288192
TCAACGC77300.031.1098884
ATCAACG77850.030.8900993
CAACGCA79050.030.4258865
AACGCAG80800.029.7623046
ACGCAGA90600.026.2827327
CGCAGAG90950.026.025828
GCAGAGT98100.023.6444179
GTACATG52300.023.5337921
TACATGG50250.023.4545022
GAGTACT48350.022.65609612-13
ACATGGG51050.022.5292663
AGAGTAC73900.022.31795910-11
GTACTTT50200.021.53673714-15
AGTACTT50850.021.26184812-13
TATAGGG6550.021.0410462
CCGTTGC1404.8308255E-420.3704689
GTATAGG2351.12664566E-720.2220881
Produced by FastQC (version 0.11.5)