FastQCFastQC Report
Thu 26 May 2016
SRR936766_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936766_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294562
Sequences flagged as poor quality0
Sequence length101
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63820.49298527223879585No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49460.38205972367488No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34240.2644910015897269No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT23210.17928843886967175No Hit
CTATAGGGAAGGAGCACAAGTAAGGAACAGTCTGCATGATCCTTCTAGAC17070.13185926977618684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCCG1201.5084333E-731.6610285
TATAGGG5600.030.5361732
GTATCAA73600.030.3415411
GGTATCA51550.029.6786961
CTATAGG6350.028.4333131
ATAGGGA7100.024.0830083
ATCAACG92250.024.0445733
TCAACGC92450.023.9897774
TATCAAC92900.023.9804482
CAACGCA93600.023.6950325
TAGGGAA7700.023.4373844
AACGCAG96650.022.9463986
TACATGG49050.022.8545742
AGAGTAC81600.022.34809710-11
GTACATG52950.022.2537561
AGTACTT58950.022.03339212-13
ACATGGG49500.021.6855183
GAGTACT58250.020.70835712-13
ACGCAGA107250.020.5013857
TACTTTT63850.020.30609514-15
Produced by FastQC (version 0.11.5)