##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936766_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1294562 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10027870430308 31.0 31.0 34.0 31.0 34.0 2 32.18548590179535 33.0 31.0 34.0 30.0 34.0 3 32.314303216068446 34.0 31.0 34.0 30.0 34.0 4 36.02651398697011 37.0 35.0 37.0 35.0 37.0 5 35.921087595650114 37.0 35.0 37.0 35.0 37.0 6 35.94737602370532 37.0 35.0 37.0 35.0 37.0 7 35.89620968327512 37.0 35.0 37.0 35.0 37.0 8 35.87081190394898 37.0 35.0 37.0 35.0 37.0 9 37.5484573160652 39.0 37.0 39.0 35.0 39.0 10-11 37.444149836006304 39.0 37.0 39.0 34.5 39.0 12-13 37.355151008603684 39.0 37.0 39.0 34.0 39.0 14-15 38.54652616097182 40.0 38.0 41.0 34.0 41.0 16-17 38.47175260821807 40.0 38.0 41.0 34.0 41.0 18-19 38.49356539122885 40.0 38.0 41.0 34.0 41.0 20-21 38.55479536708168 40.0 38.0 41.0 34.0 41.0 22-23 38.476255289433794 40.0 38.0 41.0 34.0 41.0 24-25 38.37775595143377 40.0 38.0 41.0 34.0 41.0 26-27 38.17791113905707 40.0 38.0 41.0 33.5 41.0 28-29 38.01290127471685 40.0 37.0 41.0 33.0 41.0 30-31 37.98785998662096 40.0 37.0 41.0 33.0 41.0 32-33 37.895002711341746 40.0 37.0 41.0 33.0 41.0 34-35 37.76385796895012 40.0 37.0 41.0 33.0 41.0 36-37 37.59473165441284 40.0 37.0 41.0 33.0 41.0 38-39 37.42501054410681 40.0 36.5 41.0 32.5 41.0 40-41 37.20466574795182 40.0 36.0 41.0 31.5 41.0 42-43 36.95196753805534 39.0 36.0 41.0 31.0 41.0 44-45 36.67207866444403 39.0 36.0 41.0 31.0 41.0 46-47 36.29222779596496 39.0 35.0 41.0 30.0 41.0 48-49 35.87773934349997 39.0 35.0 40.0 29.0 41.0 50-51 35.60253043114196 39.0 35.0 40.0 28.0 41.0 52-53 35.35479219998733 38.0 35.0 40.0 27.5 41.0 54-55 35.04729128461982 38.0 34.0 40.0 27.0 41.0 56-57 34.73976835408424 38.0 34.0 40.0 26.0 41.0 58-59 34.34743797516071 37.5 34.0 40.0 26.0 41.0 60-61 33.95678885986148 37.0 33.0 40.0 25.0 41.0 62-63 33.57555026333231 36.5 33.0 39.0 24.0 41.0 64-65 33.11680166728206 36.0 32.0 39.0 23.0 40.0 66-67 32.637574330159545 35.0 32.0 38.5 22.0 40.0 68-69 32.152060696977045 35.0 31.0 38.0 20.5 40.0 70-71 31.721826764573656 35.0 31.0 37.0 20.0 39.0 72-73 31.320261601993572 35.0 31.0 37.0 18.5 39.0 74-75 30.845592563353474 34.0 30.0 36.0 17.5 39.0 76-77 29.441897722936407 32.5 28.5 35.0 14.0 37.0 78-79 30.135201713011813 34.0 30.0 35.0 13.5 37.0 80-81 29.770095599901744 34.0 29.5 35.0 7.5 37.0 82-83 29.663427862087715 34.0 29.5 35.0 7.0 36.0 84-85 29.368403753547533 34.0 29.0 35.0 6.0 36.0 86-87 29.016866708585606 34.0 29.0 35.0 2.0 35.5 88-89 28.634742870561624 34.0 29.0 35.0 2.0 35.0 90-91 28.16989182441629 33.0 28.5 35.0 2.0 35.0 92-93 27.72216046817379 33.0 27.0 35.0 2.0 35.0 94-95 27.180306543834902 33.0 26.5 35.0 2.0 35.0 96-97 26.49191811593419 32.5 25.0 34.0 2.0 35.0 98-99 25.644591761537882 32.0 23.5 34.0 2.0 35.0 100-101 22.51948612735427 28.5 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.280550337773235 1101 2 0.26527980188119216 1101 3 0.27186573798269364 1101 4 0.1643553708493073 1101 5 0.2514538219357618 1101 6 0.16881434489632596 1101 7 0.1444074983442576 1101 8 0.17511145348954926 1101 9 0.35500958463561716 1101 10-11 0.22060357577787926 1101 12-13 0.2758880016785241 1101 14-15 0.2550042247397286 1101 16-17 0.27105529524060046 1101 18-19 0.3353930584162583 1101 20-21 0.25624593447808763 1101 22-23 0.3341007081898226 1101 24-25 0.32626733873289737 1101 26-27 0.22634925204434353 1101 28-29 0.2700726370997515 1101 30-31 0.14747502400487633 1101 32-33 0.3036584022787494 1101 34-35 0.4220903839995529 1101 36-37 0.46274898100428885 1101 38-39 0.42757113438960914 1101 40-41 0.557198513681918 1101 42-43 0.5514575572293268 1101 44-45 0.47856407998646944 1101 46-47 0.6282954916961287 1101 48-49 0.7370159543312624 1101 50-51 0.616909790198811 1101 52-53 0.6454031893471424 1101 54-55 0.6772403671309348 1101 56-57 0.6210321631323481 1101 58-59 0.6783999841424873 1101 60-61 0.6631834080801653 1101 62-63 0.5230475457218304 1101 64-65 0.6635988465575764 1101 66-67 0.5662868141796764 1101 68-69 0.537643128500708 1101 70-71 0.6454148763773837 1101 72-73 0.5582998440592455 1101 74-75 0.58438608151938 1101 76-77 0.693101204626636 1101 78-79 0.7660104004884403 1101 80-81 1.0753161272483567 1101 82-83 0.9291597213515779 1101 84-85 0.6814095278780954 1101 86-87 0.6513757639086357 1101 88-89 0.39653228790458783 1101 90-91 0.5393992391841707 1101 92-93 0.6903016994610027 1101 94-95 0.6700427283356909 1101 96-97 0.5835483503490764 1101 98-99 0.8871940552761934 1101 100-101 1.0188927477824272 1102 1 0.23205267300720323 1102 2 0.2221541840094332 1102 3 0.19063997437817903 1102 4 0.20417443340052444 1102 5 0.16475223247318382 1102 6 0.18470164674552336 1102 7 0.16942223606261564 1102 8 0.13830101357452662 1102 9 0.23697213742676837 1102 10-11 0.2447275555726307 1102 12-13 0.24919455976282023 1102 14-15 0.3216328068758756 1102 16-17 0.31709874642869096 1102 18-19 0.23798966060177662 1102 20-21 0.16799228984655912 1102 22-23 0.19600531611400385 1102 24-25 0.18406998292693544 1102 26-27 0.18833519724960013 1102 28-29 0.14566984979853714 1102 30-31 0.20745857414089386 1102 32-33 0.30274463610283675 1102 34-35 0.3144899775066108 1102 36-37 0.3966825464376882 1102 38-39 0.3915580375120129 1102 40-41 0.49643973054070756 1102 42-43 0.45727773302066055 1102 44-45 0.49343860417165786 1102 46-47 0.5677022574212671 1102 48-49 0.6932864562041132 1102 50-51 0.5881758686833507 1102 52-53 0.5147169381666785 1102 54-55 0.5317359032536118 1102 56-57 0.4357863672858784 1102 58-59 0.6020568756875306 1102 60-61 0.5544307234385997 1102 62-63 0.40092174627142896 1102 64-65 0.47842684294369064 1102 66-67 0.4704320859354638 1102 68-69 0.4206833380817052 1102 70-71 0.4474387458154361 1102 72-73 0.35946480783886514 1102 74-75 0.5094421926355572 1102 76-77 0.4326574618072172 1102 78-79 0.4122781441347776 1102 80-81 0.46259109894060657 1102 82-83 0.6237293868848326 1102 84-85 0.5112489233677024 1102 86-87 0.4663401515560892 1102 88-89 0.3756943425567165 1102 90-91 0.4440766717782836 1102 92-93 0.42685631248430767 1102 94-95 0.6089695681736593 1102 96-97 0.5773594759514715 1102 98-99 0.8313582089995144 1102 100-101 1.1601743336908186 1103 1 0.15988638253687526 1103 2 0.10888682233623825 1103 3 0.11987391245973811 1103 4 0.07141227162697561 1103 5 0.11980977954495842 1103 6 0.16447687043383752 1103 7 0.1751566883618949 1103 8 0.21826022841538162 1103 9 0.14216745770123396 1103 10-11 0.1572246394305452 1103 12-13 0.19257937251087753 1103 14-15 0.324217105198052 1103 16-17 0.17251782306562546 1103 18-19 0.2282302517304018 1103 20-21 0.26509777965515013 1103 22-23 0.25900751500923036 1103 24-25 0.15914991764378073 1103 26-27 0.19553792808756754 1103 28-29 0.15304382642680991 1103 30-31 0.13468439714144154 1103 32-33 0.12261888292958645 1103 34-35 0.2860436419663728 1103 36-37 0.30928618856904677 1103 38-39 0.25623015105974645 1103 40-41 0.24615524156648405 1103 42-43 0.1403433256824087 1103 44-45 0.32423311258575893 1103 46-47 0.33111318855483773 1103 48-49 0.29949719436393707 1103 50-51 0.30238361712704176 1103 52-53 0.4202927779555523 1103 54-55 0.21440048134609668 1103 56-57 0.17194095700130418 1103 58-59 0.43627662014301194 1103 60-61 0.36792980732565894 1103 62-63 0.23742965151830475 1103 64-65 0.19877446149037326 1103 66-67 0.2591994028557778 1103 68-69 0.22057618737307294 1103 70-71 0.3070444177578153 1103 72-73 0.31587662970673946 1103 74-75 0.12635431160166632 1103 76-77 0.25200356067760055 1103 78-79 0.3008217126985002 1103 80-81 0.5985488880942071 1103 82-83 0.4561675826859357 1103 84-85 0.33743140397065474 1103 86-87 0.2753777181910344 1103 88-89 0.3804821795102278 1103 90-91 0.06362544752699506 1103 92-93 0.08429323935330757 1103 94-95 0.3142900684003038 1103 96-97 0.5492077568246678 1103 98-99 0.8852326630184564 1103 100-101 0.8652531363761184 1104 1 0.28524572994738406 1104 2 0.20955517203781682 1104 3 0.13976023463931853 1104 4 0.18750155030544846 1104 5 0.0437156852463616 1104 6 0.1775228231240149 1104 7 0.17111448601829693 1104 8 0.16473388113620757 1104 9 0.2140770699708483 1104 10-11 0.2542958668299633 1104 12-13 0.23546210486454555 1104 14-15 0.309168292321381 1104 16-17 0.32216139421276324 1104 18-19 0.25612071826183325 1104 20-21 0.24952362640168957 1104 22-23 0.26808240519849136 1104 24-25 0.33569164572175936 1104 26-27 0.473528720038189 1104 28-29 0.4379990265668994 1104 30-31 0.5098668069944878 1104 32-33 0.5274629589872006 1104 34-35 0.5586569914490624 1104 36-37 0.5685325440201865 1104 38-39 0.7832085683259322 1104 40-41 0.8016206055835866 1104 42-43 0.8119618118265635 1104 44-45 0.9310650912219245 1104 46-47 0.9356468030425589 1104 48-49 1.0571182780243973 1104 50-51 1.081052973731012 1104 52-53 1.0552687728188914 1104 54-55 1.0546514043399569 1104 56-57 1.0185999381328301 1104 58-59 1.1048944642044773 1104 60-61 1.0788904505757628 1104 62-63 1.0254985878499738 1104 64-65 1.1891632863331338 1104 66-67 1.1410197072613428 1104 68-69 1.2278452200171408 1104 70-71 1.2078244022894644 1104 72-73 1.2579177229984317 1104 74-75 1.3184191181742584 1104 76-77 1.1884665606630094 1104 78-79 1.4180820626335624 1104 80-81 1.530989750819849 1104 82-83 1.4758058274653685 1104 84-85 1.2221210153647029 1104 86-87 1.142882497329186 1104 88-89 1.143872886822674 1104 90-91 1.299492332112635 1104 92-93 1.2844369783175154 1104 94-95 1.3681374570208469 1104 96-97 1.4928303435423125 1104 98-99 1.6769570928052566 1104 100-101 1.784649870510112 1105 1 0.14950329453940014 1105 2 0.11859398763109397 1105 3 0.05888866449200236 1105 4 0.07822009104222616 1105 5 0.09756680628722592 1105 6 0.16812515615091428 1105 7 0.17620026776468478 1105 8 0.039435351786060835 1105 9 0.05088164138251727 1105 10-11 0.020850228131202186 1105 12-13 0.05377418479770313 1105 14-15 0.111661397494899 1105 16-17 0.20416959406538382 1105 18-19 0.21532910099063685 1105 20-21 0.1409470537774169 1105 22-23 -0.10480601568291092 1105 24-25 0.15793420805862723 1105 26-27 0.19973294245703244 1105 28-29 0.17818575991025654 1105 30-31 0.25208193790376754 1105 32-33 0.15949857794421263 1105 34-35 0.2562621637396205 1105 36-37 0.30814748120887714 1105 38-39 0.33193632188179123 1105 40-41 0.4089614700309241 1105 42-43 0.4722181588871095 1105 44-45 0.434007561627773 1105 46-47 0.5344218160435261 1105 48-49 0.6280906272439637 1105 50-51 0.5102583865702996 1105 52-53 0.648607717312764 1105 54-55 0.610577443000274 1105 56-57 0.5125985431473836 1105 58-59 0.6092842936268354 1105 60-61 0.5604321559577983 1105 62-63 0.43748695968506723 1105 64-65 0.537271614593422 1105 66-67 0.3684534762606262 1105 68-69 0.49899952118119373 1105 70-71 0.3655594240645428 1105 72-73 0.6614178795029808 1105 74-75 0.5166860459283065 1105 76-77 0.4229325597412341 1105 78-79 0.4506559586603167 1105 80-81 0.6840293279864511 1105 82-83 0.567938947525306 1105 84-85 0.3226095683507353 1105 86-87 0.2134999385906866 1105 88-89 0.20229067510528864 1105 90-91 0.2547807071517596 1105 92-93 0.5658629689173829 1105 94-95 0.49034286493085943 1105 96-97 0.6415802172295848 1105 98-99 0.8790309629533084 1105 100-101 0.7751899714124164 1106 1 0.14495904599655063 1106 2 0.12235241409420183 1106 3 0.07635588388170333 1106 4 0.03628507599331243 1106 5 0.035927936316277 1106 6 0.07604793265466725 1106 7 0.04970826202565348 1106 8 0.10325001945091827 1106 9 0.19927526895693148 1106 10-11 0.10017009489633466 1106 12-13 -0.01593541592018255 1106 14-15 0.07267237027503626 1106 16-17 0.13023979079129333 1106 18-19 0.1674636641496079 1106 20-21 0.10989356210797752 1106 22-23 0.16698660502527218 1106 24-25 0.036201495721904564 1106 26-27 0.1501103905463026 1106 28-29 0.1408870951318164 1106 30-31 0.08648007423798276 1106 32-33 0.15405654879950959 1106 34-35 0.11820167402882475 1106 36-37 0.19386191177515855 1106 38-39 0.2805291933770633 1106 40-41 0.22332029836175593 1106 42-43 0.06842221575696783 1106 44-45 0.022557035464970454 1106 46-47 0.09736954451074098 1106 48-49 0.20129248888181905 1106 50-51 -0.07600713341577858 1106 52-53 -0.08344089550002565 1106 54-55 0.06565419162339481 1106 56-57 0.10804973176664845 1106 58-59 0.15365958197284613 1106 60-61 0.0944962756596226 1106 62-63 0.08931623519091403 1106 64-65 0.12209392585670287 1106 66-67 0.12473963041356484 1106 68-69 0.22531501269177667 1106 70-71 0.3085531019991663 1106 72-73 0.16305205144085377 1106 74-75 0.23549355669745253 1106 76-77 0.05898835817871628 1106 78-79 0.28701062297909274 1106 80-81 0.577205265945377 1106 82-83 0.45902147236319735 1106 84-85 0.4004101790950365 1106 86-87 0.5168771145996267 1106 88-89 0.21516125781348805 1106 90-91 0.4475039946933528 1106 92-93 0.43604135897918184 1106 94-95 0.2728260458034413 1106 96-97 0.5951554542014144 1106 98-99 0.677133413873225 1106 100-101 1.1490215891750353 1107 1 0.1325218598017699 1107 2 0.035880940689686724 1107 3 0.03542164922346558 1107 4 0.08168091112936793 1107 5 0.11583152860112733 1107 6 -0.006722776115104523 1107 7 0.14130719917702095 1107 8 0.053541374203028624 1107 9 0.032817893703409595 1107 10-11 0.1778054255380752 1107 12-13 0.16669473344001773 1107 14-15 0.1769638643202498 1107 16-17 0.21474058328465873 1107 18-19 0.24052606535175158 1107 20-21 0.22560761868208346 1107 22-23 0.06111698337490168 1107 24-25 0.1244447352575051 1107 26-27 0.2570853874333068 1107 28-29 0.19776511446823264 1107 30-31 0.08349740778799486 1107 32-33 0.0655231152876965 1107 34-35 -0.026122870472157445 1107 36-37 -0.10468806806954944 1107 38-39 0.17727712139208052 1107 40-41 0.14184543760210744 1107 42-43 0.09732025953299228 1107 44-45 0.16427194468174378 1107 46-47 0.215269025538376 1107 48-49 0.3354950674927295 1107 50-51 0.15438577867549697 1107 52-53 0.2042975120420678 1107 54-55 0.24519034557182806 1107 56-57 0.039200947099025996 1107 58-59 0.32850567364037175 1107 60-61 0.34114163329467573 1107 62-63 0.4304994035181906 1107 64-65 0.176963275252362 1107 66-67 0.3459286448304084 1107 68-69 0.4795238805975863 1107 70-71 0.38606021966596415 1107 72-73 0.39890905229138696 1107 74-75 0.5907041742091259 1107 76-77 0.5309656449600126 1107 78-79 0.6796683988058341 1107 80-81 0.9198868904407007 1107 82-83 0.8476297231583345 1107 84-85 0.6526541699692281 1107 86-87 0.5451362040025849 1107 88-89 0.4225201549497477 1107 90-91 0.47409842592371376 1107 92-93 0.44094985818036037 1107 94-95 0.6281432436481964 1107 96-97 0.7143708330048035 1107 98-99 0.9244092838747662 1107 100-101 1.0563958929733062 1108 1 0.05632606591859002 1108 2 -0.025144353717116985 1108 3 -0.0440615092112111 1108 4 0.0715489890964136 1108 5 -0.052067176222657 1108 6 0.02646077129988811 1108 7 0.06735552250088261 1108 8 0.05485044257380167 1108 9 -0.0015758740625173573 1108 10-11 0.033654957479335224 1108 12-13 -0.07029740146348473 1108 14-15 0.07057267194597472 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#Quality Count 2 2.0 3 4.0 4 10.0 5 28.0 6 56.0 7 155.0 8 421.0 9 736.0 10 1204.0 11 2200.0 12 3295.0 13 4375.0 14 5707.0 15 7694.0 16 10168.0 17 13815.0 18 12831.0 19 8431.0 20 7720.0 21 8185.0 22 8276.0 23 8895.0 24 10456.0 25 12179.0 26 14751.0 27 18055.0 28 22666.0 29 27756.0 30 34707.0 31 43416.0 32 55176.0 33 71426.0 34 97089.0 35 138227.0 36 208063.0 37 277187.0 38 151065.0 39 8135.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.64461717959218 18.643728606640344 14.560697259331947 23.150956954435525 2 16.375297227728584 20.721272621367024 38.32279356211306 24.58063658879133 3 19.81724816966193 24.289785566344495 29.339409610230888 26.55355665376269 4 13.98412280301971 16.752319898405716 35.30517295052145 33.95838434805312 5 14.655045218126183 36.96708311993097 33.0017002664343 15.376171395508548 6 33.301301907517754 35.147872407810524 16.637364606716403 14.913461077955322 7 29.235138989094384 32.43537196364485 20.144110517688606 18.185378529572162 8 27.27965838533306 33.966419426553315 19.21675197248791 19.537170215625718 9 27.230719317760478 16.27811766159931 19.37515449264615 37.11600852799407 10-11 25.787988524303973 26.183604956734403 26.44655103424942 21.581855484712204 12-13 26.72922586468535 23.441932609561313 27.076216213919707 22.75262531183363 14-15 23.684613019693145 24.61500492058318 25.595838592512372 26.104543467211304 16-17 22.87410722700033 27.710221681155478 26.061633201036333 23.354037890807856 18-19 22.865382037934772 26.339820078848277 28.683522703351805 22.111275179865146 20-21 23.876820564358052 25.23694517904091 28.790302116448913 22.095932140152122 22-23 23.370066478082936 25.24162612528407 28.952958606849265 22.435348789783728 24-25 23.48488389114996 25.174884597988484 28.88775674557637 22.452474765285192 26-27 23.059987377258516 25.646258871261168 28.823072270216276 22.470681481264034 28-29 22.897698391485115 25.77470903509192 28.91093453308881 22.416658040334156 30-31 23.253789743141994 25.304450490141917 28.652652530448915 22.789107236267178 32-33 23.142588545734178 25.24906203434843 28.843225906622884 22.76512351329451 34-35 23.33165961128449 25.410113401106198 28.622462379878254 22.63576460773105 36-37 23.52489216703506 25.478980780507477 28.468211776758146 22.52791527569932 38-39 23.29869272083632 25.56264762173301 28.695984688862154 22.44267496856852 40-41 23.370600154969413 25.40270029950967 28.47095559577859 22.755743949742325 42-43 23.26851498488147 25.51288667359889 28.097637739312347 23.12096060220729 44-45 23.32755131875895 25.69651646174697 28.00279483349768 22.973137385996402 46-47 23.79202889706348 25.95861342298262 27.665879248466236 22.583478431487663 48-49 23.829481365094594 26.23702910624913 27.18744906464339 22.74604046401289 50-51 23.584662619102705 26.34328384150616 27.198926863822866 22.87312667556827 52-53 23.795771609599452 26.355733417586478 26.786822674035726 23.061672298778344 54-55 23.6152664254975 26.391956289826325 26.690018825408817 23.302758459267363 56-57 23.683414680682844 26.65537060518643 26.657572139849208 23.003642574281518 58-59 23.904879651183673 26.13283617196561 26.920948175407737 23.04133600144297 60-61 23.666546548773464 26.06527383982916 27.255947284080364 23.01223232731701 62-63 23.305922507350946 26.1787485569438 27.533415755064556 22.981913180640703 64-65 23.438157857126853 26.383271955866938 27.23498569661686 22.943584490389345 66-67 23.50594239635519 26.43668260910036 26.884288675593552 23.173086318950897 68-69 23.276842311798305 26.27238050213898 27.16590114496092 23.284876041101796 70-71 23.64430776314016 26.132728927987774 26.82863710225335 23.394326206618718 72-73 23.374850029240918 26.414283164879375 26.841772254579084 23.369094551300623 74-75 23.199206797378753 26.733662984675927 26.447026996544608 23.62010322140071 76-77 23.670820072304792 26.550574730402005 26.44806569230294 23.330539504990266 78-79 23.48900350770664 26.59506788866653 26.467328808581037 23.448599795045794 80-81 23.58583115949138 26.277751728580927 26.48075522243482 23.655661889492873 82-83 23.546072828456523 26.425460450183625 26.584750489380404 23.443716231979444 84-85 23.212559388236947 26.492570604459374 26.42687227681204 23.867997730491634 86-87 23.460405913351387 26.479651032550006 26.338135987306906 23.721807066791705 88-89 23.456617987001678 26.32815303215052 26.170994355960346 24.044234624887462 90-91 23.27922861672811 26.532347925835666 26.269555427240583 23.918868030195643 92-93 23.061637206183725 26.69588639837968 26.24619658122959 23.996279814207 94-95 23.19799838322634 26.63296650157757 26.168829638945013 24.000205476251075 96-97 23.07511075234347 26.879777220743804 26.102830702442155 23.94228132447057 98-99 22.889395453892373 26.912660537418947 25.80471481385925 24.393229194829434 100-101 22.783371763824356 26.63364348091414 25.85031806542067 24.732666689840833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 474.0 1 348.0 2 256.0 3 276.0 4 250.5 5 272.0 6 426.0 7 853.0 8 1394.0 9 1692.0 10 1651.5 11 1558.5 12 1641.0 13 1747.5 14 1849.5 15 1989.0 16 2090.0 17 2189.0 18 2320.0 19 2407.0 20 2534.5 21 2810.0 22 3248.0 23 3819.0 24 4466.0 25 5255.0 26 6528.0 27 8175.0 28 9302.0 29 10390.5 30 11947.0 31 13563.0 32 15017.5 33 16326.5 34 17846.5 35 19528.5 36 21936.5 37 24232.0 38 25751.0 39 26846.0 40 27966.5 41 29798.5 42 31549.0 43 33954.0 44 37368.0 45 40671.5 46 43692.5 47 48804.0 48 54214.0 49 54570.5 50 52395.0 51 51250.0 52 52702.5 53 54801.5 54 55979.5 55 53517.0 56 47777.0 57 42696.0 58 39557.0 59 36071.5 60 30302.0 61 25293.0 62 20262.5 63 15225.5 64 10610.0 65 7100.5 66 5214.0 67 4239.5 68 3456.5 69 2923.5 70 2301.0 71 1317.0 72 830.0 73 565.0 74 305.5 75 149.0 76 73.5 77 34.0 78 18.5 79 10.5 80 5.0 81 7.0 82 6.5 83 2.0 84 1.0 85 1.0 86 1.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.027190663714831732 2 0.0077246203735317435 3 0.009964760281855948 4 7.724620373531742E-5 5 0.004557526020383728 6 0.0 7 0.0 8 3.089848149412697E-4 9 1.5449240747063484E-4 10-11 0.0 12-13 1.1586930560297614E-4 14-15 0.0 16-17 0.0 18-19 0.0039009332886335304 20-21 8.883313429561505E-4 22-23 0.0 24-25 0.005059626344663292 26-27 0.005638972872678172 28-29 0.005870711483884124 30-31 0.02664994028868451 32-33 3.862310186765872E-4 34-35 0.003244340556883332 36-37 0.015371994543328169 38-39 0.006141073196957735 40-41 0.00872882102209087 42-43 0.00957852926317936 44-45 0.0023560092139271816 46-47 0.0027808633344714273 48-49 0.0022401399083242053 50-51 0.0029739788438097213 52-53 0.0039009332886335304 54-55 0.00270361713073611 56-57 8.11085139220833E-4 58-59 9.655775466914679E-4 60-61 3.862310186765871E-5 62-63 3.862310186765871E-5 64-65 0.00872882102209087 66-67 6.565927317501981E-4 68-69 0.0015449240747063488 70-71 0.005136872548398609 72-73 0.011046207134150393 74-75 0.004866510835324998 76-77 0.003244340556883332 78-79 0.009771644772517655 80-81 2.3173861120595227E-4 82-83 0.005252741854001585 84-85 6.565927317501981E-4 86-87 0.0 88-89 8.11085139220833E-4 90-91 3.476079168089284E-4 92-93 2.3173861120595227E-4 94-95 6.565927317501981E-4 96-97 5.40723426147222E-4 98-99 3.089848149412697E-4 100-101 0.0013131854635003962 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1294562.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.29700960644762 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48071375135395 36.958601654404774 2 13.785819078202637 13.867709492182136 3 4.9759195076199365 7.508216138270102 4 2.460588865748027 4.950410472721862 5 1.3411674696012807 3.3728356551195335 6 0.8385455699572747 2.5305800752551084 7 0.5843783005977307 2.057473669927451 8 0.4215613670780087 1.6962620903703836 9 0.3096673617736728 1.401780804294034 >10 1.6380269871024613 14.88467378353123 >50 0.10973947864415377 3.803269187094751 >100 0.05063993497795065 4.589849087957062 >500 0.0021548848953270275 0.7480062720053771 >1k 9.235220979972973E-4 1.1362541972907183 >5k 1.5392034966621622E-4 0.4940774195754829 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6382 0.49298527223879585 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4946 0.38205972367488 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3424 0.2644910015897269 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2321 0.17928843886967175 No Hit CTATAGGGAAGGAGCACAAGTAAGGAACAGTCTGCATGATCCTTCTAGAC 1707 0.13185926977618684 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 7.724620373531743E-5 14-15 0.0 0.0 0.0 0.0 7.724620373531743E-5 16-17 0.0 0.0 0.0 0.0 7.724620373531743E-5 18-19 0.0 0.0 0.0 0.0 7.724620373531743E-5 20-21 0.0 0.0 0.0 0.0 1.1586930560297615E-4 22-23 0.0 0.0 0.0 1.931155093382936E-4 1.5449240747063487E-4 24-25 0.0 0.0 0.0 4.6347722241190455E-4 1.5449240747063487E-4 26-27 0.0 0.0 0.0 6.952158336178569E-4 2.3173861120595227E-4 28-29 0.0 0.0 0.0 0.0022787630101918643 2.70361713073611E-4 30-31 0.0 0.0 0.0 0.0051368725483986084 3.4760791680892847E-4 32-33 0.0 0.0 0.0 0.008303966901546623 3.8623101867658714E-4 34-35 0.0 0.0 0.0 0.011934538477106543 3.8623101867658714E-4 36-37 0.0 0.0 0.0 0.016994164821769835 3.8623101867658714E-4 38-39 0.0 0.0 0.0 0.025954724455066656 3.8623101867658714E-4 40-41 0.0 0.0 0.0 0.039395563905011886 3.8623101867658714E-4 42-43 0.0 0.0 0.0 0.06164247058078331 3.8623101867658714E-4 44-45 0.0 0.0 0.0 0.07933185123617101 3.8623101867658714E-4 46-47 0.0 0.0 0.0 0.11729836037207952 3.8623101867658714E-4 48-49 0.0 0.0 0.0 0.16391644432634359 4.6347722241190455E-4 50-51 0.0 0.0 0.0 0.18855798331790985 5.40723426147222E-4 52-53 0.0 0.0 0.0 0.2321634653264965 5.40723426147222E-4 54-55 0.0 0.0 0.0 0.2924927504437794 5.793465280148807E-4 56-57 0.0 0.0 0.0 0.38561304904670457 6.565927317501981E-4 58-59 0.0 0.0 0.0 0.4932942570537371 7.338389354855156E-4 60-61 0.0 0.0 0.0 0.6158453592798182 8.497082410884917E-4 62-63 0.0 0.0 0.0 0.7512579544278297 0.0010042006485591266 64-65 0.0 0.0 0.0 0.9072566628713032 0.0010428237504267854 66-67 0.0 0.0 0.0 1.081330982988841 0.0011200699541621026 68-69 0.0 0.0 0.0 1.298856292707495 0.0011586930560297615 70-71 0.0 0.0 0.0 1.476754299909931 0.0011586930560297615 72-73 0.0 0.0 0.0 1.674118350453667 0.0012745623616327376 74-75 0.0 0.0 0.0 1.892879599432086 0.0013131854635003962 76-77 0.0 0.0 0.0 2.1577182089386215 0.0013131854635003962 78-79 0.0 0.0 0.0 2.4366928737287203 0.0013131854635003962 80-81 0.0 0.0 0.0 2.795578736283005 0.0013131854635003962 82-83 0.0 0.0 0.0 3.1743168731972666 0.0013904316672357136 84-85 0.0 0.0 0.0 3.6452483542696292 0.0013904316672357136 86-87 0.0 0.0 0.0 4.135723124887027 0.0013904316672357136 88-89 0.0 0.0 0.0 4.681544800480781 0.0013904316672357136 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATTCCG 120 1.5084333E-7 31.661028 5 TATAGGG 560 0.0 30.536173 2 GTATCAA 7360 0.0 30.341541 1 GGTATCA 5155 0.0 29.678696 1 CTATAGG 635 0.0 28.433313 1 ATAGGGA 710 0.0 24.083008 3 ATCAACG 9225 0.0 24.044573 3 TCAACGC 9245 0.0 23.989777 4 TATCAAC 9290 0.0 23.980448 2 CAACGCA 9360 0.0 23.695032 5 TAGGGAA 770 0.0 23.437384 4 AACGCAG 9665 0.0 22.946398 6 TACATGG 4905 0.0 22.854574 2 AGAGTAC 8160 0.0 22.348097 10-11 GTACATG 5295 0.0 22.253756 1 AGTACTT 5895 0.0 22.033392 12-13 ACATGGG 4950 0.0 21.685518 3 GAGTACT 5825 0.0 20.708357 12-13 ACGCAGA 10725 0.0 20.501385 7 TACTTTT 6385 0.0 20.306095 14-15 >>END_MODULE