FastQCFastQC Report
Thu 26 May 2016
SRR936765_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936765_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398618
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65800.47046441558738694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46780.3344730298051362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30240.2162134335465438No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14790.10574724478020446No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70950.033.8256721
TCAACGC85650.028.1311914
ATCAACG85750.028.0983853
CAACGCA86500.027.8547575
TATCAAC86100.027.7633822
GGTATCA47100.027.3436131
AACGCAG88650.027.2854446
TACATGG54600.024.4580142
ACGCAGA98850.024.1763137
CGCAGAG99600.023.8988558
GTACATG58400.023.029321
ACATGGG55450.022.797523
GAGTACT49450.022.28654512-13
AGAGTAC75050.021.96498510-11
GCAGAGT108500.021.6742049
ACCGTAA1100.00287363521.5961368
GTACTTT51500.021.12118314-15
AGTACTT52050.020.4888112-13
CATGGGG33650.019.7719964
CCTACAC4850.019.597283
Produced by FastQC (version 0.11.5)