FastQCFastQC Report
Thu 26 May 2016
SRR936765_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936765_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398618
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68680.4910561711632483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51140.3656466597741485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36290.25947042008611354No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT25070.17924837232182056No Hit
CTATAGGGAAGGAGCACAAGTAAGGAACAGTCTGCATGATCCTTCTAGAC18080.1292704655595738No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG550.00258135534.5412523
GGTATCA53700.033.7078671
GTATCAA78600.031.3707831
ACCGTGC2151.8189894E-1228.7225748
TATAGGG6150.028.5737632
ATCAACG96150.025.6365093
TCAACGC97000.025.411864
CAACGCA97650.025.247225
TATCAAC97950.025.1653942
AACGCAG100150.024.711856
TACATGG55250.023.639662
GTACATG57300.023.547471
ATAGGGA7700.023.4387073
CTATAGG7700.022.8292451
GAGTACT62700.021.85340312-13
ACGCAGA112050.021.8330217
TATTCCG1754.0482737E-621.7155285
CGCAGAG112650.021.5480588
ACATGGG56950.021.516263
GTACTTT63850.021.31103314-15
Produced by FastQC (version 0.11.5)