##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936765_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1398618 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11107321656092 31.0 31.0 34.0 31.0 34.0 2 32.24834586713456 33.0 31.0 34.0 30.0 34.0 3 32.350060559781156 34.0 31.0 34.0 30.0 34.0 4 36.02674854749474 37.0 35.0 37.0 35.0 37.0 5 35.92004249909554 37.0 35.0 37.0 35.0 37.0 6 35.95174736775874 37.0 35.0 37.0 35.0 37.0 7 35.896809564870466 37.0 35.0 37.0 35.0 37.0 8 35.86319638385892 37.0 35.0 37.0 35.0 37.0 9 37.527996207685014 39.0 37.0 39.0 35.0 39.0 10-11 37.432341783103034 39.0 37.0 39.0 34.0 39.0 12-13 37.33414091624732 39.0 37.0 39.0 34.0 39.0 14-15 38.51132010313037 40.0 38.0 41.0 34.0 41.0 16-17 38.4186185934973 40.0 38.0 41.0 34.0 41.0 18-19 38.5588230667702 40.0 38.0 41.0 34.0 41.0 20-21 38.567376867736584 40.0 38.0 41.0 34.0 41.0 22-23 38.46794943294023 40.0 38.0 41.0 34.0 41.0 24-25 38.33715281799605 40.0 38.0 41.0 34.0 41.0 26-27 38.13548552928677 40.0 38.0 41.0 33.5 41.0 28-29 38.05059923438709 40.0 37.5 41.0 33.0 41.0 30-31 38.06179278401966 40.0 37.0 41.0 33.0 41.0 32-33 37.89584182385755 40.0 37.0 41.0 33.0 41.0 34-35 37.72623010714863 40.0 37.0 41.0 33.0 41.0 36-37 37.56814047867252 40.0 37.0 41.0 33.0 41.0 38-39 37.387897553156044 40.0 36.0 41.0 32.0 41.0 40-41 37.20063627094746 39.5 36.0 41.0 31.5 41.0 42-43 36.928374652692874 39.0 36.0 41.0 31.0 41.0 44-45 36.627884097015766 39.0 35.5 41.0 30.5 41.0 46-47 36.22170742833282 39.0 35.0 40.5 30.0 41.0 48-49 35.812176734462156 39.0 35.0 40.0 28.5 41.0 50-51 35.474573829308646 38.5 35.0 40.0 27.5 41.0 52-53 35.2023694103751 38.0 34.5 40.0 27.0 41.0 54-55 34.87360487281016 38.0 34.0 40.0 26.0 41.0 56-57 34.52811418128467 38.0 34.0 40.0 26.0 41.0 58-59 34.13004265639367 37.0 33.0 40.0 25.0 41.0 60-61 33.73384834172018 37.0 33.0 39.5 24.0 41.0 62-63 33.32532900334473 36.0 32.5 39.0 23.0 40.0 64-65 32.8787267145139 36.0 32.0 39.0 22.0 40.0 66-67 32.43657810781786 35.0 31.0 38.0 22.0 40.0 68-69 32.077634135982805 35.0 31.0 38.0 20.5 40.0 70-71 31.576739324104224 35.0 31.0 37.0 19.0 39.0 72-73 31.027566140289913 34.0 30.0 36.5 17.5 39.0 74-75 30.515857439272196 34.0 30.0 36.0 14.5 38.5 76-77 29.16778777335913 32.5 28.5 34.5 12.5 36.5 78-79 29.920961263189806 34.0 29.5 35.0 11.0 37.0 80-81 29.65973446645188 34.0 29.0 35.0 7.0 37.0 82-83 29.368791549944305 34.0 29.0 35.0 7.0 36.0 84-85 29.038899828259037 34.0 29.0 35.0 2.0 36.0 86-87 28.695764318777535 33.5 29.0 35.0 2.0 35.5 88-89 28.288537685057676 33.0 28.5 35.0 2.0 35.0 90-91 27.81826953464062 33.0 27.0 35.0 2.0 35.0 92-93 27.284878358493884 33.0 26.0 34.5 2.0 35.0 94-95 26.617172809158753 32.0 25.0 34.0 2.0 35.0 96-97 25.82991781887549 32.0 24.0 34.0 2.0 35.0 98-99 24.907834734001707 32.0 19.0 34.0 2.0 35.0 100-101 21.428456876716872 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19102845141931013 1101 2 0.11857922996119186 1101 3 0.12879996461322207 1101 4 0.08636592026551426 1101 5 0.16742340040206471 1101 6 0.19432730817093358 1101 7 0.2223704576302623 1101 8 0.22443839352728645 1101 9 0.28989363152950887 1101 10-11 0.3491193590884407 1101 12-13 0.3071105436828816 1101 14-15 0.3305487977905486 1101 16-17 0.3265737154274717 1101 18-19 0.23391708024009006 1101 20-21 0.3441944746204939 1101 22-23 0.3426067131622972 1101 24-25 0.2879186900420976 1101 26-27 0.2583248631279176 1101 28-29 0.34780646449718233 1101 30-31 0.4299599932374676 1101 32-33 0.4755022802323694 1101 34-35 0.5105208821217104 1101 36-37 0.49729751576312964 1101 38-39 0.5189967254852874 1101 40-41 0.6263047434826916 1101 42-43 0.6701317593453453 1101 44-45 0.837645391845868 1101 46-47 0.7440589898151941 1101 48-49 0.7375043131097954 1101 50-51 0.7410446470424787 1101 52-53 0.6572001131811831 1101 54-55 0.5188596913494692 1101 56-57 0.5419514444688929 1101 58-59 0.6158906621843911 1101 60-61 0.8192734005871714 1101 62-63 0.6765064845557376 1101 64-65 0.7537524947879888 1101 66-67 0.4290265085201952 1101 68-69 0.3189500004830528 1101 70-71 0.36042070922628255 1101 72-73 0.6211817301616698 1101 74-75 0.4517691982172778 1101 76-77 0.38179456363419817 1101 78-79 0.609835931082408 1101 80-81 0.7439351982154037 1101 82-83 0.6802543013081284 1101 84-85 0.48871514108376246 1101 86-87 0.23834631431871856 1101 88-89 0.284078448124216 1101 90-91 0.4963349728017903 1101 92-93 0.6561337715168207 1101 94-95 0.5992573536745667 1101 96-97 0.5204491370239488 1101 98-99 0.5801824526693515 1101 100-101 0.884345512078788 1102 1 0.26689207650110447 1102 2 0.31606563534037235 1102 3 0.29752218307483247 1102 4 0.23783143167145937 1102 5 0.20020392342830462 1102 6 0.124826642542196 1102 7 0.19219829670605293 1102 8 0.260490651024071 1102 9 0.22487411394111945 1102 10-11 0.3307818514731977 1102 12-13 0.29790455979335206 1102 14-15 0.43343018049322524 1102 16-17 0.47082561641801846 1102 18-19 0.3318164010731337 1102 20-21 0.3871015799252149 1102 22-23 0.346338746827314 1102 24-25 0.347725996163625 1102 26-27 0.2734380200132236 1102 28-29 0.33970158848464393 1102 30-31 0.3996208610368299 1102 32-33 0.36226416565396846 1102 34-35 0.3485226420892218 1102 36-37 0.354004571427744 1102 38-39 0.4946685592360538 1102 40-41 0.44262280120059927 1102 42-43 0.5691615208471816 1102 44-45 0.5296984616353484 1102 46-47 0.5729337387941911 1102 48-49 0.7131152249083428 1102 50-51 0.7008519531640047 1102 52-53 0.7197466507835699 1102 54-55 0.6459654473457732 1102 56-57 0.5886582744966447 1102 58-59 0.805256396970826 1102 60-61 0.6605604332368173 1102 62-63 0.809363724114391 1102 64-65 0.7584525105825719 1102 66-67 0.7279096060630152 1102 68-69 0.5113798838287984 1102 70-71 0.5263517997292269 1102 72-73 0.7695221710560922 1102 74-75 0.7763591032241592 1102 76-77 0.6181469181548316 1102 78-79 0.661800099949037 1102 80-81 0.9170437972361398 1102 82-83 0.7841081788775703 1102 84-85 0.8048459202078426 1102 86-87 0.5897757238270565 1102 88-89 0.820236754945217 1102 90-91 0.8908542534714812 1102 92-93 0.8744870737122667 1102 94-95 0.7259569633227976 1102 96-97 0.7350433291311695 1102 98-99 0.8766828249701675 1102 100-101 0.8772898474654163 1103 1 0.2545961801980532 1103 2 0.22090738358476614 1103 3 0.2193787899484434 1103 4 0.16465579073397407 1103 5 0.12779392987802396 1103 6 0.13623484932892183 1103 7 0.10456567265910621 1103 8 0.10468672050853911 1103 9 0.23839999453807792 1103 10-11 0.256717230772999 1103 12-13 0.27027803178111753 1103 14-15 0.3919080783448763 1103 16-17 0.3402522263684915 1103 18-19 0.3272068061027298 1103 20-21 0.38983439767608985 1103 22-23 0.3764308597940058 1103 24-25 0.2689240461004161 1103 26-27 0.3606104814800162 1103 28-29 0.39190799329807646 1103 30-31 0.4318763377153303 1103 32-33 0.4138372112836066 1103 34-35 0.4977745496426138 1103 36-37 0.5225440686779237 1103 38-39 0.5274553897366943 1103 40-41 0.6756450940777228 1103 42-43 0.7788229920711203 1103 44-45 0.6094347018786195 1103 46-47 0.6202180054234532 1103 48-49 0.7955503570121181 1103 50-51 0.8488970249616656 1103 52-53 0.9541273454188328 1103 54-55 0.8638557305609282 1103 56-57 0.7535541918383046 1103 58-59 0.8723652993825155 1103 60-61 0.7006063417213184 1103 62-63 0.7627982765180548 1103 64-65 0.6673289351853526 1103 66-67 0.6273679150903817 1103 68-69 0.5042273650173819 1103 70-71 0.6834649508463748 1103 72-73 0.8512967729050338 1103 74-75 1.0749684149475343 1103 76-77 0.8482606161846569 1103 78-79 1.0328968200060977 1103 80-81 1.0924775363291914 1103 82-83 0.9227858341932524 1103 84-85 0.8219675657000458 1103 86-87 0.7221836165526305 1103 88-89 0.6611546878369232 1103 90-91 0.7591716310107799 1103 92-93 0.8064731014064179 1103 94-95 0.5322358031757481 1103 96-97 0.5416870051251017 1103 98-99 0.6039689100878753 1103 100-101 0.8996997575291701 1104 1 0.2691527439074868 1104 2 0.32625973914648654 1104 3 0.27929342919697575 1104 4 0.1941462384425492 1104 5 0.21569699183571345 1104 6 0.15606203946791197 1104 7 0.29620615225938707 1104 8 0.2713256603871912 1104 9 0.26540264144344405 1104 10-11 0.2957117429927294 1104 12-13 0.2560114489791374 1104 14-15 0.18125734596831222 1104 16-17 0.283572860832912 1104 18-19 0.32139559579658084 1104 20-21 0.22742225914786474 1104 22-23 0.2533510873471698 1104 24-25 0.27103957052960226 1104 26-27 0.14716993278694446 1104 28-29 0.11549760828272326 1104 30-31 0.37613337070359165 1104 32-33 0.33108743834830534 1104 34-35 0.3230933074164355 1104 36-37 0.40171892654569064 1104 38-39 0.47113440768453785 1104 40-41 0.39721448809284254 1104 42-43 0.3380383853231024 1104 44-45 0.3567254112336897 1104 46-47 0.464840624960722 1104 48-49 0.432305954696659 1104 50-51 0.6214482835336668 1104 52-53 0.5398153319797174 1104 54-55 0.5978597041046072 1104 56-57 0.632065730547609 1104 58-59 0.6877348007179975 1104 60-61 0.7472650395911202 1104 62-63 0.5808759181317384 1104 64-65 0.6725071783169625 1104 66-67 0.730972863578728 1104 68-69 0.7657089984390417 1104 70-71 0.6176099062581599 1104 72-73 0.8395080800543688 1104 74-75 0.6033174333838431 1104 76-77 0.5470937195626284 1104 78-79 0.5709989131072213 1104 80-81 0.9188221557630953 1104 82-83 0.7784264893571979 1104 84-85 0.8439242616485636 1104 86-87 0.6008677987622271 1104 88-89 0.46521024382509424 1104 90-91 0.6331496628996511 1104 92-93 0.6321248461057039 1104 94-95 0.5924519269991002 1104 96-97 0.7865592011504283 1104 98-99 0.7065300334970921 1104 100-101 0.9213846129687759 1105 1 0.22459615113107034 1105 2 0.24386012358354492 1105 3 0.14258175511250215 1105 4 0.17614264560794624 1105 5 0.1840574278564162 1105 6 0.22517295368851364 1105 7 0.19841028308061937 1105 8 0.16855538593141972 1105 9 0.27576019794049245 1105 10-11 0.23731668323416244 1105 12-13 0.2715609236631664 1105 14-15 0.22904683289126382 1105 16-17 0.21628581699995664 1105 18-19 0.1826620136804067 1105 20-21 0.2647936520001153 1105 22-23 0.3519491966977313 1105 24-25 0.21278107227440302 1105 26-27 0.3257212827279048 1105 28-29 0.22119316153982993 1105 30-31 0.18563687812859797 1105 32-33 0.11361113247306776 1105 34-35 0.3715017792979012 1105 36-37 0.3009937896556849 1105 38-39 0.38745106706166865 1105 40-41 0.349279288637959 1105 42-43 0.3834354648779197 1105 44-45 0.3235496476624746 1105 46-47 0.5302404451312768 1105 48-49 0.3881369901050391 1105 50-51 0.3866962435582195 1105 52-53 0.3630198063164798 1105 54-55 0.40452873908876086 1105 56-57 0.3543526617525288 1105 58-59 0.22944659681618162 1105 60-61 0.2941150209136367 1105 62-63 0.3430514789724697 1105 64-65 0.32139347467513346 1105 66-67 0.3169064186641677 1105 68-69 0.35116106863634755 1105 70-71 0.29223898456655917 1105 72-73 0.1767345112283074 1105 74-75 0.30278615751458915 1105 76-77 0.10410650472275762 1105 78-79 0.2857425810647314 1105 80-81 0.26249278818833943 1105 82-83 0.3627961571232383 1105 84-85 0.34880739128156435 1105 86-87 0.11315856870630725 1105 88-89 0.1665295875039412 1105 90-91 0.12606770534916123 1105 92-93 -0.15198385309670215 1105 94-95 -0.46975653061615574 1105 96-97 -0.35838933721913335 1105 98-99 -0.3527622963453929 1105 100-101 0.03492048593935948 1106 1 0.20235112447428705 1106 2 0.1919503520831185 1106 3 0.12022177869325645 1106 4 0.2527094622444537 1106 5 0.19646622260493984 1106 6 0.20749316648043248 1106 7 0.19936969618180456 1106 8 0.20475942368631195 1106 9 0.12377285140484418 1106 10-11 0.12142259884434736 1106 12-13 0.21071204214361927 1106 14-15 0.3225340619084349 1106 16-17 0.19701086102121224 1106 18-19 -4.314449717099933E-4 1106 20-21 0.21614742626960748 1106 22-23 0.1541961113562067 1106 24-25 0.22682292426276973 1106 26-27 0.24811381754356177 1106 28-29 0.4496587042247171 1106 30-31 0.26111359855408267 1106 32-33 0.363899791234104 1106 34-35 0.3360628959674159 1106 36-37 0.48902238196419745 1106 38-39 0.6355139388021271 1106 40-41 0.6361161922638274 1106 42-43 0.5416679870555186 1106 44-45 0.45715145397918633 1106 46-47 0.6868771563607723 1106 48-49 0.8285727845620201 1106 50-51 0.8188402428595296 1106 52-53 0.8920420526274881 1106 54-55 0.7429613894449716 1106 56-57 0.8866792321011303 1106 58-59 1.0273450069132721 1106 60-61 0.844172110488401 1106 62-63 1.12498664216902 1106 64-65 0.8942847887114596 1106 66-67 1.051834835144497 1106 68-69 0.9943480154807496 1106 70-71 0.6694764913254794 1106 72-73 0.6332539608565533 1106 74-75 0.6915741888682589 1106 76-77 0.6212863567907618 1106 78-79 0.8462084037389914 1106 80-81 0.8956958023123889 1106 82-83 0.9085696020584528 1106 84-85 0.8873406066254965 1106 86-87 0.6941146681718884 1106 88-89 0.7255200075008332 1106 90-91 0.6702459815137196 1106 92-93 0.6495078608877414 1106 94-95 0.828317193923116 1106 96-97 0.9970619786666681 1106 98-99 0.7845005635790052 1106 100-101 0.9606346835835957 1107 1 0.10662713309604399 1107 2 0.16314523644373935 1107 3 0.1266159378650329 1107 4 0.14525966027498782 1107 5 0.11553776869413923 1107 6 0.08132778192205592 1107 7 0.15215168004448287 1107 8 0.1965027555140395 1107 9 0.2701317340710645 1107 10-11 0.2537389437552733 1107 12-13 0.1474257415506628 1107 14-15 0.24914438133945538 1107 16-17 0.2860311851874968 1107 18-19 0.17091704274898945 1107 20-21 0.0914455252232429 1107 22-23 0.0806710819656189 1107 24-25 0.14257491373442832 1107 26-27 0.14063554161450753 1107 28-29 0.1137515979825281 1107 30-31 0.16188945544474365 1107 32-33 0.2096386046735006 1107 34-35 0.15812775730704232 1107 36-37 0.306366611852809 1107 38-39 0.28670734875772297 1107 40-41 0.23336159020886527 1107 42-43 0.2142100407535068 1107 44-45 0.2557280290377193 1107 46-47 0.22294059634273822 1107 48-49 0.3021089695038981 1107 50-51 0.20318682009456523 1107 52-53 0.12311389047726351 1107 54-55 0.35114293317045053 1107 56-57 0.35046737648049486 1107 58-59 0.22355145638213259 1107 60-61 0.2468371950950612 1107 62-63 0.19280556806886295 1107 64-65 0.16307721198583636 1107 66-67 0.2973131313563684 1107 68-69 0.2510130673228623 1107 70-71 0.2919340202694549 1107 72-73 0.1473815304363768 1107 74-75 0.0788606049172742 1107 76-77 0.0658461922814837 1107 78-79 0.3732432582058536 1107 80-81 0.5103775999631708 1107 82-83 0.3197446950668841 1107 84-85 0.20815631925849587 1107 86-87 0.3088670656402819 1107 88-89 0.11081675686680015 1107 90-91 0.4455870254392913 1107 92-93 0.5972928598697003 1107 94-95 0.8795847531899064 1107 96-97 0.688846188178843 1107 98-99 0.3270517757505722 1107 100-101 0.5339642957994712 1108 1 0.06068438442691004 1108 2 0.12799202058430836 1108 3 0.12199172136824643 1108 4 0.12795404014394762 1108 5 0.15355171700012704 1108 6 0.09864257253698128 1108 7 0.22989812533566578 1108 8 0.15326025582353964 1108 9 0.10760737497326289 1108 10-11 0.13437179884786588 1108 12-13 0.1404765781280517 1108 14-15 0.19689807131290138 1108 16-17 0.22238091993124698 1108 18-19 0.2769273778843271 1108 20-21 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10 1361.0 11 2397.0 12 3471.0 13 4735.0 14 6263.0 15 8529.0 16 11403.0 17 15272.0 18 14063.0 19 9353.0 20 8616.0 21 9131.0 22 9404.0 23 10164.0 24 11990.0 25 13864.0 26 17068.0 27 20768.0 28 25574.0 29 32026.0 30 39566.0 31 48989.0 32 62374.0 33 81112.0 34 109077.0 35 155093.0 36 229667.0 37 290097.0 38 139879.0 39 5781.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.570342006812815 18.636049281707738 14.579255310399377 23.214353401080068 2 16.340202020779813 20.712671309030874 38.299075698088544 24.64805097210077 3 19.8348507879944 24.189519052433337 29.271407264620546 26.70422289495172 4 13.984672000983828 16.681693415709947 35.31903301618671 34.01460156711952 5 14.659256494625408 36.90242796818002 33.06892947180717 15.369386065387403 6 33.263979156567416 35.160279647480586 16.611969815918286 14.96377138003372 7 29.299565714154973 32.31797388564998 20.115857224774743 18.266603175420308 8 27.225875828853912 33.96581482577802 19.23698965693277 19.5713196884353 9 27.228755263235037 16.28472984383859 19.349757653453377 37.13675723947299 10-11 25.791515620435764 26.189387392599112 26.414960093046503 21.604136893918618 12-13 26.731530696730633 23.434275835145836 27.033471612691955 22.800721855431576 14-15 23.701861408905074 24.53697149614834 25.560052852172642 26.201114242773937 16-17 22.90925756711268 27.71732524534934 26.008281031704154 23.36513615583383 18-19 22.87616677927807 26.3416308276402 28.60297508606729 22.17922730701444 20-21 23.858244211632684 25.249990615632388 28.744764863497668 22.147000309237256 22-23 23.42577167003911 25.20835269807449 28.822885906520142 22.54298972536626 24-25 23.475276856729547 25.191978073132937 28.855167837353328 22.477577232784185 26-27 23.037400752791505 25.66301854521325 28.76785448700348 22.531726214991764 28-29 22.975553236587313 25.77733400399897 28.82507317083634 22.422039588577377 30-31 23.277502895794306 25.336341146019535 28.615274063693175 22.770881894492987 32-33 23.133269172467838 25.234203842787768 28.821909267193302 22.810617717551096 34-35 23.429699889462313 25.444581722814952 28.509500092233903 22.61621829548883 36-37 23.579566377952364 25.385180826014174 28.48600535288191 22.54924744315155 38-39 23.279900404089954 25.529389302878684 28.652022845276665 22.538687447754693 40-41 23.400907761013467 25.37583851349129 28.401958807555523 22.82129491793972 42-43 23.30607785685584 25.488267704262352 28.031850011940357 23.17380442694145 44-45 23.322951656563838 25.677061213283398 28.043647371905696 22.95633975824707 46-47 23.91507887621723 25.946730892085778 27.57205250478062 22.56613772691637 48-49 23.88993916652128 26.273298764740034 27.066786693856304 22.76997537488238 50-51 23.57553216325763 26.339359612903273 27.19004857657792 22.89505964726118 52-53 23.84929265889614 26.353836429961575 26.727705492135804 23.069165419006477 54-55 23.741925658138406 26.333723256535624 26.577294171315476 23.347056914010494 56-57 23.756895016282765 26.63016257026401 26.59752195912309 23.015420454330137 58-59 23.924353600443297 26.125999874875994 26.87388393854624 23.07576258613447 60-61 23.679786869691494 26.06352438650318 27.23585548495225 23.02083325885308 62-63 23.344661724885256 26.202476706248113 27.433715323931697 23.019146244934934 64-65 23.51851865092445 26.363364666667145 27.13659279130741 22.981523891100995 66-67 23.568114996968344 26.45354989646612 26.822246341993573 23.156088764571965 68-69 23.35997414568506 26.263186344116928 27.087471382219153 23.28936812797886 70-71 23.712392803671094 26.16008700116259 26.734124504326463 23.393395690839853 72-73 23.510136434680522 26.456187465054793 26.688774204250194 23.344901896014495 74-75 23.244588586733475 26.74801125110645 26.39473394450808 23.612666217651995 76-77 23.744832398839424 26.52904510023466 26.41253723318304 23.313585267742873 78-79 23.572665797926824 26.48541106946165 26.446085397484232 23.4958377351273 80-81 23.50460717628393 26.277671836914468 26.52342127259168 23.694299714209922 82-83 23.631452205455133 26.352833490953103 26.51805714714774 23.497657156444028 84-85 23.292918111982768 26.51894946166668 26.397710948220322 23.79042147813023 86-87 23.50564948724879 26.480300085661128 26.28062671340132 23.73342371368876 88-89 23.57408630842578 26.388590974480415 26.067772906281373 23.969549810812435 90-91 23.305098076227328 26.537209192581756 26.220250735375078 23.937441995815835 92-93 23.197740617933846 26.707719257478395 26.176835971364476 23.917704153223283 94-95 23.322236665050784 26.560361728506283 26.13293980200455 23.984461804438382 96-97 23.11368562624747 26.844682876673886 26.08530577968161 23.956325717397032 98-99 23.029193466103383 26.816990190431962 25.75970615983958 24.394110183625077 100-101 22.895998810246464 26.68812370004769 25.822978402112383 24.59289908759346 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 466.0 1 342.5 2 255.5 3 304.5 4 286.5 5 274.5 6 427.5 7 905.0 8 1501.5 9 1799.0 10 1748.5 11 1673.5 12 1723.5 13 1834.0 14 2006.5 15 2098.5 16 2156.5 17 2264.0 18 2354.0 19 2514.5 20 2722.5 21 2952.0 22 3436.5 23 4063.5 24 4767.0 25 5676.5 26 6976.0 27 8572.0 28 9822.5 29 11062.0 30 12551.0 31 14343.0 32 16187.0 33 17614.5 34 19407.0 35 21203.5 36 23738.0 37 26330.5 38 27754.5 39 28662.5 40 29703.5 41 31958.5 42 34133.5 43 36532.5 44 40202.5 45 43958.5 46 47622.0 47 53310.0 48 58376.5 49 58721.5 50 56915.0 51 55755.0 52 57250.5 53 59547.0 54 61001.0 55 58076.5 56 51529.5 57 46129.0 58 42852.0 59 39075.0 60 32856.0 61 27297.0 62 21825.5 63 16515.0 64 11618.5 65 7834.0 66 5726.0 67 4624.0 68 3728.0 69 3094.0 70 2507.5 71 1519.5 72 924.5 73 640.5 74 348.5 75 161.5 76 88.0 77 40.0 78 26.5 79 17.5 80 10.5 81 6.0 82 4.0 83 3.5 84 2.5 85 1.0 86 0.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02652618513418246 2 0.0035034584139486263 3 2.8599660522029603E-4 4 7.149915130507401E-5 5 0.0 6 0.0 7 0.0 8 0.0 9 7.149915130507401E-5 10-11 0.012691099356650637 12-13 0.0 14-15 0.0 16-17 0.0 18-19 2.1449745391522202E-4 20-21 0.0011082368452286471 22-23 0.0020019762365420723 24-25 0.010796371847066177 26-27 0.00643492361745666 28-29 5.362436347880551E-4 30-31 0.002716967749592812 32-33 0.0035749575652537003 34-35 0.0 36-37 0.008508399005303808 38-39 0.01194035826794736 40-41 8.579898156608881E-4 42-43 0.0 44-45 0.0 46-47 1.7874787826268503E-4 48-49 0.003288960960033404 50-51 0.007471661311380234 52-53 0.0 54-55 0.0037179558678638487 56-57 0.003825204594821459 58-59 3.932453321779071E-4 60-61 0.002645468598287738 62-63 0.004790443137439959 64-65 7.149915130507401E-5 66-67 0.003002964354813108 68-69 8.579898156608881E-4 70-71 0.0011439864208811841 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0028957156278554973 80-81 0.0027884669008978865 82-83 0.01505057134971808 84-85 0.009652385426184991 86-87 0.006005928709626216 88-89 4.2899490783044404E-4 90-91 8.579898156608881E-4 92-93 3.932453321779071E-4 94-95 0.0 96-97 0.0026812181739402753 98-99 0.0024667207200250534 100-101 6.43492361745666E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1398618.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.43865088347129 #Duplication Level Percentage of deduplicated Percentage of total 1 73.01317660059593 36.09672947850097 2 13.957059155405183 13.80036349888067 3 5.052844746603851 7.494174821871892 4 2.4836225415165876 4.911477910254329 5 1.391877797779228 3.4406280258431052 6 0.8685736036514001 2.5764664294512083 7 0.5829923745313227 2.0175649530525996 8 0.42522631124392907 1.6818092118443928 9 0.31960427635512423 1.4220723815626917 >10 1.731458762057414 15.38786980859964 >50 0.11574956862764056 3.932098830487906 >100 0.054481633623254415 4.848640950035829 >500 0.0023183499194585526 0.7882721747508512 >1k 7.244843498307975E-4 0.7435002636985719 >5k 2.897937399323191E-4 0.858331261165458 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6868 0.4910561711632483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5114 0.3656466597741485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3629 0.25947042008611354 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2507 0.17924837232182056 No Hit CTATAGGGAAGGAGCACAAGTAAGGAACAGTCTGCATGATCCTTCTAGAC 1808 0.1292704655595738 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.149915130507401E-5 2 0.0 0.0 0.0 0.0 7.149915130507401E-5 3 0.0 0.0 0.0 0.0 7.149915130507401E-5 4 0.0 0.0 0.0 0.0 7.149915130507401E-5 5 0.0 0.0 0.0 0.0 7.149915130507401E-5 6 0.0 0.0 0.0 0.0 7.149915130507401E-5 7 0.0 0.0 0.0 0.0 7.149915130507401E-5 8 0.0 0.0 0.0 0.0 7.149915130507401E-5 9 0.0 0.0 0.0 0.0 7.149915130507401E-5 10-11 0.0 0.0 0.0 0.0 7.149915130507401E-5 12-13 0.0 0.0 0.0 0.0 1.0724872695761101E-4 14-15 0.0 0.0 0.0 0.0 1.78747878262685E-4 16-17 0.0 0.0 0.0 0.0 2.8599660522029603E-4 18-19 0.0 0.0 0.0 0.0 2.8599660522029603E-4 20-21 0.0 0.0 0.0 3.5749575652537004E-5 3.5749575652537006E-4 22-23 0.0 0.0 0.0 1.0724872695761101E-4 3.5749575652537006E-4 24-25 0.0 0.0 0.0 2.1449745391522202E-4 4.2899490783044404E-4 26-27 0.0 0.0 0.0 5.362436347880551E-4 5.004940591355181E-4 28-29 0.0 0.0 0.0 0.001966226660889535 5.004940591355181E-4 30-31 0.0 0.0 0.0 0.00489769186439757 5.004940591355181E-4 32-33 0.0 0.0 0.0 0.007364412584422623 5.719932104405921E-4 34-35 0.0 0.0 0.0 0.010045630758362898 6.43492361745666E-4 36-37 0.0 0.0 0.0 0.014836073895802857 6.43492361745666E-4 38-39 0.0 0.0 0.0 0.023666219081979495 7.149915130507401E-4 40-41 0.0 0.0 0.0 0.03499883456383373 7.149915130507401E-4 42-43 0.0 0.0 0.0 0.052802123238797155 7.149915130507401E-4 44-45 0.0 0.0 0.0 0.06971167252244716 7.149915130507401E-4 46-47 0.0 0.0 0.0 0.10127854782363734 7.149915130507401E-4 48-49 0.0 0.0 0.0 0.1414253212814364 7.149915130507401E-4 50-51 0.0 0.0 0.0 0.16387605479122963 7.864906643558141E-4 52-53 0.0 0.0 0.0 0.2020923511637917 7.864906643558141E-4 54-55 0.0 0.0 0.0 0.2540722341625805 7.864906643558141E-4 56-57 0.0 0.0 0.0 0.3362247590121105 8.222402400083511E-4 58-59 0.0 0.0 0.0 0.4349650869644177 8.579898156608881E-4 60-61 0.0 0.0 0.0 0.542964555010732 8.579898156608881E-4 62-63 0.0 0.0 0.0 0.6684813151267894 8.579898156608881E-4 64-65 0.0 0.0 0.0 0.806653425023845 8.937393913134251E-4 66-67 0.0 0.0 0.0 0.9639873074706604 9.652385426184992E-4 68-69 0.0 0.0 0.0 1.1541035507908521 0.0010009881182710362 70-71 0.0 0.0 0.0 1.3104364451193964 0.0010367376939235731 72-73 0.0 0.0 0.0 1.4875755924777172 0.0010724872695761101 74-75 0.0 0.0 0.0 1.6814097916657729 0.0011082368452286471 76-77 0.0 0.0 0.0 1.913674784680306 0.0011439864208811841 78-79 0.0 0.0 0.0 2.164887052790683 0.0011797359965337211 80-81 0.0 0.0 0.0 2.48302252652261 0.001286984723491332 82-83 0.0 0.0 0.0 2.815672327969467 0.0015014821774065542 84-85 0.0 0.0 0.0 3.234299858860675 0.0015014821774065542 86-87 0.0 0.0 0.0 3.6819560451817437 0.0016444804800167022 88-89 0.0 0.0 0.0 4.188277285148626 0.0017159796313217762 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATCG 55 0.002581355 34.541252 3 GGTATCA 5370 0.0 33.707867 1 GTATCAA 7860 0.0 31.370783 1 ACCGTGC 215 1.8189894E-12 28.722574 8 TATAGGG 615 0.0 28.573763 2 ATCAACG 9615 0.0 25.636509 3 TCAACGC 9700 0.0 25.41186 4 CAACGCA 9765 0.0 25.24722 5 TATCAAC 9795 0.0 25.165394 2 AACGCAG 10015 0.0 24.71185 6 TACATGG 5525 0.0 23.63966 2 GTACATG 5730 0.0 23.54747 1 ATAGGGA 770 0.0 23.438707 3 CTATAGG 770 0.0 22.829245 1 GAGTACT 6270 0.0 21.853403 12-13 ACGCAGA 11205 0.0 21.833021 7 TATTCCG 175 4.0482737E-6 21.715528 5 CGCAGAG 11265 0.0 21.548058 8 ACATGGG 5695 0.0 21.51626 3 GTACTTT 6385 0.0 21.311033 14-15 >>END_MODULE