FastQCFastQC Report
Thu 26 May 2016
SRR936764_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936764_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448397
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68230.4710725029118398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48830.3371313251822532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32340.2232813241121046No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15240.10521977054633502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44550.037.349631
GTATCAA71900.037.2259251
ATCAACG87850.030.1997593
TCAACGC88300.030.0458534
TATCAAC89300.029.8691222
CAACGCA89100.029.7227155
AACGCAG91200.028.9821726
ACGCAGA101250.026.0548677
CGCAGAG101100.025.9045688
GCAGAGT107600.023.719639
GAGTACT55300.023.63447412-13
TACATGG56900.023.5639382
GTACATG60300.022.7848681
GTACTTT57900.022.24367514-15
AGAGTAC82800.022.1558210-11
ACATGGG57650.021.9378133
AGTACTT58000.021.34607912-13
GTATTAG2902.413799E-919.6720331
CAGAGTA101050.019.6359110-11
TACTTTT63750.019.15882114-15
Produced by FastQC (version 0.11.5)