FastQCFastQC Report
Thu 26 May 2016
SRR936764_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936764_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448397
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68080.47003687524898213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54890.37897068276170137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36380.2511742291650701No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26010.17957783673951272No Hit
CTATAGGGAAGGAGCACAAGTAAGGAACAGTCTGCATGATCCTTCTAGAC18270.12613944933605908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61450.032.085151
GTATCAA87200.029.4208471
TATAGGG7100.026.7602122
TATCAAC105150.024.3482322
ATCAACG105250.024.1880363
TCAACGC105250.024.1880364
CAACGCA106150.023.9829565
ATACCGT1201.711267E-423.749696
CTATAGG8050.023.6070651
AACGCAG108350.023.5852956
GAGTACT63750.022.3553512-13
ATAGGGA8950.021.757993
GTACTTT65250.021.54798314-15
GTACATG61400.021.5106891
TACATGG58850.021.3888452
ACGCAGA122200.020.8344257
CGCAGAG122050.020.591168
AGAGTAC92250.019.95575710-11
ACATGGG59750.019.7933523
GTCTAGA8650.019.7726231
Produced by FastQC (version 0.11.5)