##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936764_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1448397 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02479085499348 31.0 31.0 34.0 30.0 34.0 2 32.17405725087804 33.0 31.0 34.0 30.0 34.0 3 32.289983340202994 34.0 31.0 34.0 30.0 34.0 4 35.988639164538455 37.0 35.0 37.0 35.0 37.0 5 35.80112496780924 37.0 35.0 37.0 35.0 37.0 6 35.894073931387595 37.0 35.0 37.0 35.0 37.0 7 35.846561405471014 37.0 35.0 37.0 35.0 37.0 8 35.80556919131978 37.0 35.0 37.0 35.0 37.0 9 37.47721860788168 39.0 37.0 39.0 35.0 39.0 10-11 37.370433313518326 39.0 37.0 39.0 34.0 39.0 12-13 37.25940746908479 39.0 37.0 39.0 34.0 39.0 14-15 38.418275859450134 40.0 38.0 41.0 34.0 41.0 16-17 38.410662960500474 40.0 38.0 41.0 33.5 41.0 18-19 38.517308099920115 40.0 38.0 41.0 34.0 41.0 20-21 38.503088586899864 40.0 38.0 41.0 34.0 41.0 22-23 38.38982716755144 40.0 38.0 41.0 34.0 41.0 24-25 38.280262593750194 40.0 38.0 41.0 34.0 41.0 26-27 38.06573750152755 40.0 37.0 41.0 33.0 41.0 28-29 37.89319986164014 40.0 37.0 41.0 33.0 41.0 30-31 37.89375254160289 40.0 37.0 41.0 33.0 41.0 32-33 37.75754161324554 40.0 37.0 41.0 33.0 41.0 34-35 37.614150333092375 40.0 37.0 41.0 33.0 41.0 36-37 37.464201458578 40.0 36.5 41.0 32.5 41.0 38-39 37.26727547764874 39.5 36.0 41.0 32.0 41.0 40-41 36.855507502432 39.0 35.5 41.0 30.5 41.0 42-43 36.66741542546691 39.0 35.0 41.0 30.5 41.0 44-45 36.440441053109055 39.0 35.0 40.0 30.0 41.0 46-47 36.03725843121741 39.0 35.0 40.0 29.5 41.0 48-49 35.62151122931075 39.0 35.0 40.0 28.0 41.0 50-51 35.307899698770434 38.0 35.0 40.0 27.0 41.0 52-53 35.01976771561941 38.0 34.0 40.0 26.5 41.0 54-55 34.614177604620835 38.0 34.0 40.0 26.0 41.0 56-57 34.3355554450886 38.0 33.5 40.0 25.5 41.0 58-59 33.96569863096927 37.0 33.0 40.0 25.0 41.0 60-61 33.5116466686965 36.5 32.5 39.0 23.5 40.5 62-63 33.08126225061223 36.0 32.0 39.0 22.5 40.0 64-65 32.70303825539545 35.5 32.0 39.0 22.0 40.0 66-67 32.3210946308229 35.0 31.0 38.0 21.0 40.0 68-69 31.81735359849544 35.0 31.0 37.5 19.5 40.0 70-71 31.34099766845692 34.5 30.5 37.0 18.0 39.0 72-73 30.893874055248666 34.0 30.0 36.5 17.5 39.0 74-75 30.402620621279937 34.0 29.5 36.0 13.5 38.5 76-77 29.036442356619077 32.5 28.0 34.5 11.0 36.5 78-79 29.71444017075429 34.0 29.0 35.0 9.0 37.0 80-81 29.603379114980214 34.0 29.0 35.0 7.0 36.5 82-83 29.26964326769525 34.0 29.0 35.0 6.0 36.0 84-85 28.834609571823194 33.5 29.0 35.0 2.0 36.0 86-87 28.4390940467289 33.0 28.5 35.0 2.0 35.0 88-89 28.040232753865133 33.0 27.0 35.0 2.0 35.0 90-91 27.536706441673104 33.0 26.5 34.5 2.0 35.0 92-93 26.991180594823106 32.0 25.5 34.0 2.0 35.0 94-95 26.360840294477274 32.0 25.0 34.0 2.0 35.0 96-97 25.48922256812186 32.0 23.5 34.0 2.0 35.0 98-99 24.532175225438884 31.0 16.0 34.0 2.0 35.0 100-101 21.378407301313107 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2815487990430867 1101 2 0.34277643146229053 1101 3 0.34552701412813036 1101 4 0.1924161017723236 1101 5 0.09336263137359424 1101 6 0.14609841925307876 1101 7 0.07920188085381596 1101 8 0.23276420404162224 1101 9 0.29497562538324473 1101 10-11 0.33164853878825085 1101 12-13 0.36698348070687814 1101 14-15 0.5359104073617473 1101 16-17 0.4699608921642664 1101 18-19 0.3556999673287109 1101 20-21 0.2975822792927403 1101 22-23 0.28190126651611536 1101 24-25 0.21526752203660493 1101 26-27 0.262401723903146 1101 28-29 0.3367440095093812 1101 30-31 0.46751325253436704 1101 32-33 0.5285431025174674 1101 34-35 0.29789145608018686 1101 36-37 0.3696483623378981 1101 38-39 0.3911712581252331 1101 40-41 0.5569100316041968 1101 42-43 0.5988155166116229 1101 44-45 0.6184735701774073 1101 46-47 0.4984412632582078 1101 48-49 0.679369730125444 1101 50-51 0.8648178431130731 1101 52-53 0.8910564405881232 1101 54-55 0.7101503345475351 1101 56-57 0.7773006750998022 1101 58-59 0.8364767493782708 1101 60-61 0.7775322936293918 1101 62-63 0.775976087206466 1101 64-65 0.872802349201308 1101 66-67 0.7477152365313628 1101 68-69 0.5014767315532538 1101 70-71 0.5048376807076345 1101 72-73 0.6112572377676067 1101 74-75 0.531747049731262 1101 76-77 0.5772939290357328 1101 78-79 0.6996813572883838 1101 80-81 0.627386070650882 1101 82-83 1.0069825772845356 1101 84-85 0.7769327342154888 1101 86-87 0.5411519720665403 1101 88-89 0.7794205173258817 1101 90-91 0.7428795563811725 1101 92-93 0.45430547703810475 1101 94-95 0.5382759956918584 1101 96-97 0.6437601909999024 1101 98-99 0.8811245399213234 1101 100-101 1.1637788468746955 1102 1 -0.008652252842473729 1102 2 0.08468679738417961 1102 3 0.12657535610284043 1102 4 0.18004618868343414 1102 5 0.013286910533572893 1102 6 0.1922303152581506 1102 7 0.14960267909321345 1102 8 0.2623438228263595 1102 9 0.2449411220448141 1102 10-11 0.2680854629555114 1102 12-13 0.34109198904362614 1102 14-15 0.4067023988571492 1102 16-17 0.29221274788436347 1102 18-19 0.29813301220170985 1102 20-21 0.2531289240762149 1102 22-23 0.1783935825294023 1102 24-25 0.2217578797523032 1102 26-27 0.27886774277740045 1102 28-29 0.13771569812141848 1102 30-31 0.12456871898220356 1102 32-33 0.034010016613486016 1102 34-35 0.3712366946194834 1102 36-37 0.3477107667543251 1102 38-39 0.4695311372702946 1102 40-41 0.43126192835529054 1102 42-43 0.6297706063202995 1102 44-45 0.4759980955233729 1102 46-47 0.48074897708565345 1102 48-49 0.6656243461126152 1102 50-51 0.5814918145405699 1102 52-53 0.5513011345341923 1102 54-55 0.7734965988617546 1102 56-57 0.6227560259148319 1102 58-59 0.48134880726927776 1102 60-61 0.6041926502260608 1102 62-63 0.6097655795411328 1102 64-65 0.5886300563145923 1102 66-67 0.5807843551109428 1102 68-69 0.8037912527961169 1102 70-71 0.48552047379950736 1102 72-73 0.2783233349737735 1102 74-75 0.39065170861447385 1102 76-77 0.5094412489656044 1102 78-79 0.18979894839584688 1102 80-81 0.47786305599163015 1102 82-83 0.7453027309642728 1102 84-85 0.5721344388669074 1102 86-87 0.5483370591641581 1102 88-89 0.5090122589052015 1102 90-91 0.4613882661428228 1102 92-93 0.3363879866430892 1102 94-95 0.4831778943079925 1102 96-97 0.5664074363279639 1102 98-99 0.41901992952929135 1102 100-101 0.2926884481280041 1103 1 0.17796299743078947 1103 2 0.21025469816641618 1103 3 0.24012482758872267 1103 4 0.16558563336195675 1103 5 0.05155334759734842 1103 6 0.06404014788008539 1103 7 0.06194398746333718 1103 8 0.17927520453507384 1103 9 0.2873778133205107 1103 10-11 0.20625549428164902 1103 12-13 0.23180482665453894 1103 14-15 0.3056712467513307 1103 16-17 0.2478951142597552 1103 18-19 0.2335004836998067 1103 20-21 0.22033937100801637 1103 22-23 0.1628095148942421 1103 24-25 0.2537883998395003 1103 26-27 0.242996640988828 1103 28-29 0.19298771038318563 1103 30-31 0.32429576021912965 1103 32-33 0.2627429719569463 1103 34-35 0.20765462990322447 1103 36-37 0.21400783992802985 1103 38-39 0.24817623592015536 1103 40-41 0.3804201528409763 1103 42-43 0.3262723680340116 1103 44-45 0.29554258413787693 1103 46-47 0.28298829230723044 1103 48-49 0.3975593001004327 1103 50-51 0.22145790842128577 1103 52-53 0.13836429365021985 1103 54-55 0.2747710170687725 1103 56-57 0.16148006582664465 1103 58-59 0.1611817650553249 1103 60-61 0.15140634884782855 1103 62-63 0.43006001777734326 1103 64-65 0.409096009746726 1103 66-67 0.5703358966867711 1103 68-69 0.43004628299256353 1103 70-71 0.3134228742489391 1103 72-73 0.41908471894187116 1103 74-75 0.06428905206010072 1103 76-77 0.4151859775639899 1103 78-79 0.34832990204170855 1103 80-81 0.31305069224201887 1103 82-83 0.5362271574150128 1103 84-85 0.1622018999840229 1103 86-87 0.03866580043955281 1103 88-89 0.3145390632114804 1103 90-91 0.07454432894165919 1103 92-93 0.21830187334382956 1103 94-95 0.43949640115639 1103 96-97 0.5013357770195839 1103 98-99 0.3725132063168033 1103 100-101 0.9910522984953083 1104 1 0.07494185259823283 1104 2 0.19327202547655276 1104 3 0.07310875157760677 1104 4 0.09859235644312747 1104 5 -0.0626685140810892 1104 6 0.035094314281643335 1104 7 0.13685120045987986 1104 8 0.08424063474863885 1104 9 0.1021489740594177 1104 10-11 0.21703654876617406 1104 12-13 0.22796821661444966 1104 14-15 0.21654058144200405 1104 16-17 0.2178718909983175 1104 18-19 0.25363738429576443 1104 20-21 0.25188748239484227 1104 22-23 0.15460714378864537 1104 24-25 0.10317176392509708 1104 26-27 0.17121741043167304 1104 28-29 0.19565149269957516 1104 30-31 0.2149144639581806 1104 32-33 0.3220237931484391 1104 34-35 0.35307280767251115 1104 36-37 0.3200994392578451 1104 38-39 0.38051144620201427 1104 40-41 0.5595340634685328 1104 42-43 0.523569084964727 1104 44-45 0.528631755099255 1104 46-47 0.4500881417271714 1104 48-49 0.5174475315271323 1104 50-51 0.6513801264018184 1104 52-53 0.6311709149307845 1104 54-55 0.8676346360800409 1104 56-57 0.8207319904942096 1104 58-59 0.4947855062273021 1104 60-61 0.7764617559441831 1104 62-63 0.6674253440694713 1104 64-65 0.7333266729352061 1104 66-67 0.5435626044978505 1104 68-69 0.6945443848355808 1104 70-71 0.5997025926006181 1104 72-73 0.7963651583811178 1104 74-75 0.4066579837900761 1104 76-77 0.5319536495115642 1104 78-79 0.6430333547786624 1104 80-81 0.5318151405039835 1104 82-83 0.5942227460557596 1104 84-85 0.40208467023279937 1104 86-87 0.485545771171882 1104 88-89 0.655377539595861 1104 90-91 0.7254547019984336 1104 92-93 0.5474497175040796 1104 94-95 0.5253577826753393 1104 96-97 0.7210848279410875 1104 98-99 0.7003784689835548 1104 100-101 1.0675588943257246 1105 1 0.045972876922068195 1105 2 0.2045804287395825 1105 3 0.17271602852048318 1105 4 0.10477701338876244 1105 5 0.029850071796829525 1105 6 0.1489241750603938 1105 7 0.1378270255239613 1105 8 0.2719486955422781 1105 9 0.21712748995446418 1105 10-11 0.23715729049958156 1105 12-13 0.2760761263812128 1105 14-15 0.3442460364902331 1105 16-17 0.021910530480141688 1105 18-19 0.12468356448571427 1105 20-21 0.17890666918955844 1105 22-23 0.11213615264818344 1105 24-25 0.2568455990505498 1105 26-27 0.16077914000847926 1105 28-29 0.07081767544209328 1105 30-31 0.2188038048641303 1105 32-33 0.3642395275963892 1105 34-35 0.29110326298664546 1105 36-37 0.26441226697021847 1105 38-39 0.18251800310830646 1105 40-41 0.3556371446295259 1105 42-43 0.39094528546885954 1105 44-45 0.29857169000879935 1105 46-47 0.2072047514928741 1105 48-49 0.38037202872462217 1105 50-51 0.5305420070055007 1105 52-53 0.76184505849141 1105 54-55 0.881715632219283 1105 56-57 0.5863804222609019 1105 58-59 0.5642392569053314 1105 60-61 0.4706356548838073 1105 62-63 0.5944531723667978 1105 64-65 0.5259040210308825 1105 66-67 0.6308650464535361 1105 68-69 0.6429054241557424 1105 70-71 0.29716092726341614 1105 72-73 0.08988110660655124 1105 74-75 0.16684232698269952 1105 76-77 0.21151277334296736 1105 78-79 0.4633692034539827 1105 80-81 0.29716976118190175 1105 82-83 0.40386772653926073 1105 84-85 0.018140715030352084 1105 86-87 0.08422441894955313 1105 88-89 0.2642139413273519 1105 90-91 0.2964732293800303 1105 92-93 0.4059923081467929 1105 94-95 0.5461665209627782 1105 96-97 0.7446595117950388 1105 98-99 0.623316207621972 1105 100-101 0.6843195539515641 1106 1 0.031007149726665517 1106 2 0.09135509034133804 1106 3 0.043401138228098546 1106 4 0.09855749039846984 1106 5 -0.07789277248031823 1106 6 0.028032196936486287 1106 7 0.1658841558452906 1106 8 0.12171988945206635 1106 9 0.23631525719959257 1106 10-11 0.14669517532294662 1106 12-13 0.27482818063269576 1106 14-15 0.2955261190066807 1106 16-17 0.25748843423918544 1106 18-19 0.13054804354293736 1106 20-21 0.16915210172737005 1106 22-23 0.2838311650987748 1106 24-25 0.19102199334366787 1106 26-27 0.3368703468499561 1106 28-29 0.21649441419518922 1106 30-31 0.36827537633283924 1106 32-33 0.0797396488309019 1106 34-35 0.19979731710726156 1106 36-37 0.27875354020201826 1106 38-39 0.2711096429301847 1106 40-41 0.46244956212270694 1106 42-43 0.48467688380159757 1106 44-45 0.24795050745271396 1106 46-47 0.23598447800320344 1106 48-49 0.2276723477830771 1106 50-51 0.4584305636220165 1106 52-53 0.31600802077014833 1106 54-55 0.4269130135537864 1106 56-57 0.4504101420359845 1106 58-59 0.5568121996710929 1106 60-61 0.5632184118721071 1106 62-63 0.4726278809228788 1106 64-65 0.4678182562720252 1106 66-67 0.6358905057014255 1106 68-69 0.49813698207697854 1106 70-71 0.29953558424671556 1106 72-73 0.594748768550847 1106 74-75 0.4101907857648861 1106 76-77 0.6236783681425813 1106 78-79 0.6326145089842008 1106 80-81 0.6158972221463124 1106 82-83 0.8364266984705857 1106 84-85 0.8005747961689558 1106 86-87 0.5203011161365261 1106 88-89 0.6032231666965409 1106 90-91 0.34332459586618924 1106 92-93 0.6069625691281395 1106 94-95 0.8030797747816649 1106 96-97 0.9287339151480367 1106 98-99 0.9957409363838039 1106 100-101 1.7434502447573657 1107 1 0.27712256870037777 1107 2 0.2598870597884684 1107 3 0.21940674034721752 1107 4 0.1959729798644787 1107 5 0.20950515084351906 1107 6 0.15713868378050933 1107 7 0.2352766281788803 1107 8 0.19765696601953664 1107 9 0.1884726216778887 1107 10-11 0.22838368839093448 1107 12-13 0.28655878205309904 1107 14-15 0.3393669451225776 1107 16-17 0.25120157717085334 1107 18-19 0.3166006094728999 1107 20-21 0.3075413682658805 1107 22-23 0.15792892062817288 1107 24-25 0.35973200500957603 1107 26-27 0.511401148671375 1107 28-29 0.465499913836517 1107 30-31 0.5834513017124436 1107 32-33 0.35443990129891034 1107 34-35 0.4598600666380932 1107 36-37 0.34613837949304127 1107 38-39 0.331930728097511 1107 40-41 0.4571675567048388 1107 42-43 0.44380299669485623 1107 44-45 0.5994612267329273 1107 46-47 0.4490966145822881 1107 48-49 0.36343937332902954 1107 50-51 0.08283549373527421 1107 52-53 0.35386476510256415 1107 54-55 0.41010743255129967 1107 56-57 0.2684504768795719 1107 58-59 0.17797381128441003 1107 60-61 0.3950461498146609 1107 62-63 0.4100271499146544 1107 64-65 0.4502497850646634 1107 66-67 0.24326807838561137 1107 68-69 0.18087325289989664 1107 70-71 0.11040557926774497 1107 72-73 0.4842189687344778 1107 74-75 0.4426356159674718 1107 76-77 0.3304700059550214 1107 78-79 0.5481619422261339 1107 80-81 0.505520362666136 1107 82-83 0.5525285469007599 1107 84-85 0.4610340519317795 1107 86-87 0.663144802314342 1107 88-89 0.4991649945069412 1107 90-91 0.5856887066954037 1107 92-93 0.8444651957684322 1107 94-95 1.0059384392787045 1107 96-97 0.8049482510660901 1107 98-99 0.9836788348438432 1107 100-101 1.1660127484450165 1108 1 -0.04955604630178101 1108 2 -0.013292367058362231 1108 3 0.013211952665237447 1108 4 -0.003403688545134287 1108 5 -0.10556339728464792 1108 6 -0.003945977400469758 1108 7 0.05128544838968452 1108 8 0.067130574187523 1108 9 0.11216782216224885 1108 10-11 0.042996720562001656 1108 12-13 -0.06722088238443291 1108 14-15 -0.04252427457085162 1108 16-17 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-1.5931834046287534 2316 74-75 -1.535121652069808 2316 76-77 -1.3016476764334186 2316 78-79 -1.4945568006845384 2316 80-81 -1.3641143953053252 2316 82-83 -1.5741865597471723 2316 84-85 -1.210855662242956 2316 86-87 -1.2551816844650325 2316 88-89 -2.1282466000791374 2316 90-91 -2.1318550369905083 2316 92-93 -1.8226384589367584 2316 94-95 -2.269785916337348 2316 96-97 -2.273507218587927 2316 98-99 -2.261390667063239 2316 100-101 -2.832548577583154 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 11.0 5 28.0 6 83.0 7 169.0 8 490.0 9 932.0 10 1612.0 11 2597.0 12 3854.0 13 5311.0 14 6705.0 15 9050.0 16 11726.0 17 15972.0 18 14534.0 19 9993.0 20 9436.0 21 9648.0 22 10233.0 23 11175.0 24 12757.0 25 15168.0 26 18530.0 27 22925.0 28 28182.0 29 35004.0 30 42597.0 31 53049.0 32 67192.0 33 86867.0 34 116613.0 35 163758.0 36 240555.0 37 290724.0 38 126605.0 39 4308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.53569421023047 18.63848364691535 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26.367353025753047 28.56171829232635 22.187785553836793 20-21 23.916662955632955 25.24352148446872 28.716807392517246 22.123008167381084 22-23 23.411916884698595 25.263407539560756 28.807072208929537 22.517603366811116 24-25 23.511158243871993 25.148078108742737 28.850324256364473 22.490439391020793 26-27 23.099162555505423 25.551084321295058 28.79339848363136 22.55635463956816 28-29 22.955558094762036 25.797666591986378 28.837683603902658 22.409091709348925 30-31 23.32972244488217 25.28264004965489 28.619639504914744 22.767998000548193 32-33 23.213057754923376 25.257491735685544 28.777730675864888 22.75171983352619 34-35 23.441328586016127 25.382370993588083 28.532888427689368 22.64341199270642 36-37 23.576104986441727 25.427383399700698 28.48321656474227 22.513295049115303 38-39 23.34654799137313 25.515728987537162 28.623929797402482 22.51379322368723 40-41 23.40563762930283 25.348584724099304 28.36731065646915 22.87846699012871 42-43 23.27013933334576 25.48752172229023 28.064232389324197 23.17810655503981 44-45 23.33799599140285 25.69551507987851 27.97487826112772 22.991610667590916 46-47 23.856690977492665 25.976728025410207 27.58847813907054 22.578102858026593 48-49 23.899533035325618 26.19300032829408 27.100831850946893 22.806634785433406 50-51 23.63069205109059 26.310534922569772 27.184035002142064 22.874738024197573 52-53 23.839248049389912 26.336015156107496 26.700108326952698 23.124628467549897 54-55 23.66164111082804 26.39745870779904 26.621361408508854 23.31953877286407 56-57 23.72033309985643 26.619577746256283 26.606977587943064 23.053111565944224 58-59 23.884740045105083 26.09173392212279 26.899455293806145 23.12407073896598 60-61 23.687268403926335 26.04166882426005 27.191200056891425 23.079862714922193 62-63 23.318710270733785 26.17207160743912 27.507064706706796 23.0021534151203 64-65 23.532782632380016 26.349863452445515 27.14494960461448 22.972404310559988 66-67 23.57019518819771 26.435155554727054 26.835287562733146 23.15936169434209 68-69 23.295554773848185 26.237670416905868 27.142878348573753 23.3238964606722 70-71 23.71220714877012 26.117569244174042 26.757556153768082 23.412667453287757 72-73 23.516198319376468 26.419627020604242 26.714816648266936 23.34935801175235 74-75 23.23930898368846 26.688797895231787 26.41786525934516 23.654027861734598 76-77 23.713308968441286 26.52970131431643 26.420131794112866 23.336857923129422 78-79 23.537335356265654 26.589960939455292 26.4054805586193 23.46722314565976 80-81 23.41837918301854 26.303995702713973 26.54523473510656 23.732390379160925 82-83 23.612042696872024 26.36042685825325 26.5365871845175 23.49094326035723 84-85 23.29524525607871 26.495810587677664 26.344190144187866 23.86475401205576 86-87 23.444293088961352 26.577938446264238 26.256513252963803 23.721255211810607 88-89 23.53868805656101 26.364425664421002 26.075820892430855 24.02106538658713 90-91 23.319753667321084 26.48110820016895 26.209008562404613 23.990129570105356 92-93 23.187496245849722 26.674454586691354 26.165374548552638 23.972674618906282 94-95 23.321023172514167 26.51310379681814 26.16775649217721 23.998116538490482 96-97 23.1129869058374 26.83600830851561 26.078893615581677 23.972111170065315 98-99 22.965127317843415 26.798988941460927 25.84747956859477 24.388404172100888 100-101 22.8288182981563 26.653856815739406 25.876773422712308 24.640551463391986 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 488.0 1 350.5 2 240.0 3 292.0 4 289.5 5 301.5 6 451.0 7 891.0 8 1492.5 9 1786.5 10 1766.5 11 1699.0 12 1755.0 13 1868.0 14 2021.0 15 2148.0 16 2231.5 17 2380.0 18 2480.0 19 2610.5 20 2833.5 21 3099.5 22 3579.0 23 4305.5 24 4978.5 25 5918.0 26 7362.5 27 8957.0 28 10208.0 29 11355.0 30 12992.0 31 14926.0 32 16670.0 33 18108.0 34 19944.0 35 21861.0 36 24620.0 37 27341.5 38 28863.0 39 29695.0 40 30862.0 41 33161.5 42 35163.0 43 37736.0 44 41713.0 45 45640.0 46 49092.5 47 54807.5 48 60401.0 49 61108.5 50 59214.5 51 57809.0 52 59317.0 53 61577.5 54 62732.0 55 59896.5 56 53696.5 57 47983.5 58 44478.0 59 40582.5 60 34156.0 61 28448.0 62 22539.0 63 16979.5 64 11930.0 65 8086.5 66 5933.5 67 4793.0 68 3939.0 69 3299.0 70 2562.5 71 1488.5 72 945.5 73 668.0 74 374.5 75 165.0 76 81.5 77 45.0 78 27.0 79 16.0 80 11.5 81 9.5 82 4.5 83 2.5 84 2.5 85 1.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020091176659437986 2 0.0034520922095254273 3 2.0712553257152562E-4 4 0.0 5 0.0023474227024772904 6 0.0 7 2.7616737676203413E-4 8 0.0 9 2.7616737676203413E-4 10-11 0.0 12-13 0.007663644705146448 14-15 0.005557868457335937 16-17 0.0 18-19 3.7973014304779697E-4 20-21 9.320648965718653E-4 22-23 0.0016224833384769508 24-25 4.1425106514305125E-4 26-27 1.7260461047627137E-4 28-29 3.4520922095254266E-5 30-31 0.0 32-33 6.904184419050853E-5 34-35 0.0 36-37 0.0013463159617149165 38-39 0.009079002511051874 40-41 3.1068829885728846E-4 42-43 0.0 44-45 6.904184419050855E-4 46-47 1.7260461047627137E-4 48-49 3.4520922095254266E-5 50-51 0.0019676925594294932 52-53 2.7616737676203413E-4 54-55 0.0 56-57 1.7260461047627137E-4 58-59 1.0356276628576281E-4 60-61 0.001657004260572205 62-63 0.0 64-65 3.4520922095254266E-5 66-67 0.0 68-69 3.4520922095254266E-5 70-71 5.523347535240683E-4 72-73 4.1425106514305125E-4 74-75 0.004591282638668818 76-77 1.0356276628576281E-4 78-79 3.1068829885728846E-4 80-81 0.00283071561181085 82-83 3.4520922095254266E-5 84-85 0.0030033202222871217 86-87 2.7616737676203413E-4 88-89 0.003417571287430173 90-91 0.0021057762478105106 92-93 0.0 94-95 0.0 96-97 2.416464546667799E-4 98-99 9.665858186671196E-4 100-101 0.002243859936191528 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1448397.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.35150619236183 #Duplication Level Percentage of deduplicated Percentage of total 1 72.84213532695473 35.94869092653065 2 14.05623109204998 13.873923515611471 3 5.165305305285309 7.647467902776821 4 2.39823344212821 4.734257302796784 5 1.3775158332007031 3.3991240586140483 6 0.8845822155897332 2.6193278814197916 7 0.5906860036126054 2.040587077552027 8 0.44477728677604245 1.75603432180398 9 0.3292470676874126 1.4623954829813084 >10 1.737353309404892 15.344359796652206 >50 0.11601907564626517 3.9434613672180574 >100 0.054681010279904865 4.848561546818648 >500 0.002249065310725058 0.7989541612106296 >1k 7.028329096015806E-4 0.7297905510059758 >5k 2.8113316384063223E-4 0.8530641070076506 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6808 0.47003687524898213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5489 0.37897068276170137 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3638 0.2511742291650701 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2601 0.17957783673951272 No Hit CTATAGGGAAGGAGCACAAGTAAGGAACAGTCTGCATGATCCTTCTAGAC 1827 0.12613944933605908 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.904184419050855E-5 2 0.0 0.0 0.0 0.0 6.904184419050855E-5 3 0.0 0.0 0.0 0.0 6.904184419050855E-5 4 0.0 0.0 0.0 0.0 6.904184419050855E-5 5 0.0 0.0 0.0 0.0 6.904184419050855E-5 6 0.0 0.0 0.0 0.0 6.904184419050855E-5 7 0.0 0.0 0.0 0.0 6.904184419050855E-5 8 0.0 0.0 0.0 0.0 6.904184419050855E-5 9 0.0 0.0 0.0 0.0 6.904184419050855E-5 10-11 0.0 0.0 0.0 0.0 6.904184419050855E-5 12-13 0.0 0.0 0.0 0.0 6.904184419050855E-5 14-15 0.0 0.0 0.0 0.0 1.380836883810171E-4 16-17 0.0 0.0 0.0 0.0 2.761673767620342E-4 18-19 0.0 0.0 0.0 0.0 3.1068829885728846E-4 20-21 0.0 0.0 0.0 0.0 3.7973014304779697E-4 22-23 0.0 0.0 0.0 0.0 4.1425106514305125E-4 24-25 0.0 0.0 0.0 1.380836883810171E-4 4.1425106514305125E-4 26-27 0.0 0.0 0.0 3.452092209525427E-4 4.832929093335598E-4 28-29 0.0 0.0 0.0 0.0021057762478105106 6.213765977145769E-4 30-31 0.0 0.0 0.0 0.00476388724914509 6.213765977145769E-4 32-33 0.0 0.0 0.0 0.008146937614480008 7.594602860955939E-4 34-35 0.0 0.0 0.0 0.011840676278672215 7.594602860955939E-4 36-37 0.0 0.0 0.0 0.01688073090457934 7.594602860955939E-4 38-39 0.0 0.0 0.0 0.023508747946868157 7.939812081908483E-4 40-41 0.0 0.0 0.0 0.03541846606973088 8.285021302861025E-4 42-43 0.0 0.0 0.0 0.05554416365126412 8.285021302861025E-4 44-45 0.0 0.0 0.0 0.07038816015222346 8.630230523813567E-4 46-47 0.0 0.0 0.0 0.1054614170010018 8.97543974476611E-4 48-49 0.0 0.0 0.0 0.15109807601092795 8.97543974476611E-4 50-51 0.0 0.0 0.0 0.17529724239970118 8.97543974476611E-4 52-53 0.0 0.0 0.0 0.21389163330219546 0.001035627662857628 54-55 0.0 0.0 0.0 0.2658801419776484 0.001035627662857628 56-57 0.0 0.0 0.0 0.34914460607140174 0.001035627662857628 58-59 0.0 0.0 0.0 0.44694237836725703 0.001035627662857628 60-61 0.0 0.0 0.0 0.5607923794374057 0.001035627662857628 62-63 0.0 0.0 0.0 0.6905220046713711 0.001035627662857628 64-65 0.0 0.0 0.0 0.8347158962632483 0.001035627662857628 66-67 0.0 0.0 0.0 0.9962047698248477 0.0010701485849528825 68-69 0.0 0.0 0.0 1.1993603963554191 0.0011046695070481367 70-71 0.0 0.0 0.0 1.3567758011097786 0.0011046695070481367 72-73 0.0 0.0 0.0 1.5292768488197641 0.0011737113512386454 74-75 0.0 0.0 0.0 1.721869073189188 0.001277274117524408 76-77 0.0 0.0 0.0 1.9517093724993906 0.0013117950396196623 78-79 0.0 0.0 0.0 2.197981630726935 0.0013117950396196623 80-81 0.0 0.0 0.0 2.5205106058628957 0.0013117950396196623 82-83 0.0 0.0 0.0 2.8539136714588613 0.0013117950396196623 84-85 0.0 0.0 0.0 3.26926940610896 0.0013117950396196623 86-87 0.0 0.0 0.0 3.7296749440933663 0.0013463159617149165 88-89 0.0 0.0 0.0 4.252528830148088 0.0013808368838101708 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6145 0.0 32.08515 1 GTATCAA 8720 0.0 29.420847 1 TATAGGG 710 0.0 26.760212 2 TATCAAC 10515 0.0 24.348232 2 ATCAACG 10525 0.0 24.188036 3 TCAACGC 10525 0.0 24.188036 4 CAACGCA 10615 0.0 23.982956 5 ATACCGT 120 1.711267E-4 23.74969 6 CTATAGG 805 0.0 23.607065 1 AACGCAG 10835 0.0 23.585295 6 GAGTACT 6375 0.0 22.35535 12-13 ATAGGGA 895 0.0 21.75799 3 GTACTTT 6525 0.0 21.547983 14-15 GTACATG 6140 0.0 21.510689 1 TACATGG 5885 0.0 21.388845 2 ACGCAGA 12220 0.0 20.834425 7 CGCAGAG 12205 0.0 20.59116 8 AGAGTAC 9225 0.0 19.955757 10-11 ACATGGG 5975 0.0 19.793352 3 GTCTAGA 865 0.0 19.772623 1 >>END_MODULE