FastQCFastQC Report
Thu 26 May 2016
SRR936763_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936763_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770237
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195030.7040191868060386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139810.5046860611565003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93140.3362167208076421No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43640.15753164801423125No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33600.12128926153249704No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30710.11085694112092215No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA211750.030.9485651
GGTATCA142300.030.1232151
ATCAACG250800.026.0156573
TCAACGC252650.025.8251634
TATCAAC254650.025.7347662
TACATGG159100.025.6579912
GTACATG163350.025.4849991
CAACGCA257200.025.3687615
AACGCAG260600.025.128056
ACATGGG160550.024.2122293
GAGTACT136350.023.27656712-13
ACGCAGA288750.022.5137627
CGCAGAG288850.022.3406438
GTATAGG9350.021.8552881
GTACTTT145550.021.57698814-15
CATGGGG58250.021.374564
CATGGGA95150.021.2261854
AGTACTT142900.021.14572112-13
GCAGAGT301500.020.9304719
GTGTAGC19550.020.6619741
Produced by FastQC (version 0.11.5)