##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936763_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2770237 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.10424920322701 33.0 31.0 34.0 31.0 34.0 2 32.216694817086044 33.0 31.0 34.0 31.0 34.0 3 32.230739824787555 34.0 31.0 34.0 30.0 34.0 4 35.81185219892738 37.0 35.0 37.0 35.0 37.0 5 35.84659326981771 37.0 35.0 37.0 35.0 37.0 6 35.91503651131654 37.0 35.0 37.0 35.0 37.0 7 35.876252104061855 37.0 35.0 37.0 35.0 37.0 8 35.85851715936218 37.0 35.0 37.0 35.0 37.0 9 37.537606349204054 39.0 37.0 39.0 35.0 39.0 10-11 37.429843908661965 39.0 37.0 39.0 35.0 39.0 12-13 37.33782795479232 39.0 37.0 39.0 35.0 39.0 14-15 38.54229439575026 40.0 38.0 41.0 34.5 41.0 16-17 38.4808177062107 40.0 38.0 41.0 34.0 41.0 18-19 38.48554401663107 40.0 38.0 41.0 34.0 41.0 20-21 38.44532760193442 40.0 38.0 41.0 34.0 41.0 22-23 38.314473815778214 40.0 38.0 41.0 34.0 41.0 24-25 38.16201646285137 40.0 38.0 41.0 34.0 41.0 26-27 38.12187657590307 40.0 38.0 41.0 34.0 41.0 28-29 38.203001042871065 40.0 38.0 41.0 34.0 41.0 30-31 38.061619457107824 40.0 38.0 41.0 34.0 41.0 32-33 37.85827512230903 40.0 37.0 41.0 33.5 41.0 34-35 37.66790801653433 40.0 37.0 41.0 33.0 41.0 36-37 37.51742901419625 40.0 37.0 41.0 33.0 41.0 38-39 37.360605247854245 40.0 37.0 41.0 33.0 41.0 40-41 37.172063256681646 40.0 36.5 41.0 32.0 41.0 42-43 36.95164529244249 39.5 36.0 41.0 31.0 41.0 44-45 36.66306348518195 39.0 36.0 41.0 31.0 41.0 46-47 36.272583898056375 39.0 36.0 41.0 30.0 41.0 48-49 35.88503781445414 39.0 35.0 40.5 29.0 41.0 50-51 35.230215320927414 38.5 34.5 39.5 28.0 40.5 52-53 35.53169530260407 38.5 35.5 40.0 28.5 40.5 54-55 35.853964841275314 39.0 36.0 41.0 29.0 41.0 56-57 35.75023256132959 39.0 35.0 41.0 28.0 41.0 58-59 35.53614817071608 39.0 35.0 41.0 28.0 41.0 60-61 35.270840545411815 39.0 35.0 40.5 27.5 41.0 62-63 34.98864302945921 38.0 35.0 40.0 26.5 41.0 64-65 34.68914338376103 38.0 34.5 40.0 26.0 41.0 66-67 34.31999121374814 37.0 34.0 40.0 26.0 41.0 68-69 33.885321183711 37.0 34.0 39.5 25.0 41.0 70-71 33.53188734393483 36.0 34.0 39.0 25.0 41.0 72-73 33.15250121199017 36.0 33.0 39.0 24.0 40.0 74-75 32.72827469274289 35.0 33.0 38.0 22.5 40.0 76-77 32.294519385886474 35.0 32.5 37.0 21.5 39.0 78-79 31.870770804086437 35.0 32.0 37.0 20.0 39.0 80-81 31.457517714188352 35.0 32.0 36.0 19.5 38.5 82-83 31.04807927985945 35.0 31.5 36.0 18.0 37.0 84-85 30.698936589179915 35.0 31.0 35.0 15.0 37.0 86-87 30.384251419643878 34.0 31.0 35.0 9.5 36.5 88-89 30.023089360224414 34.0 31.0 35.0 7.0 36.0 90-91 29.707451925593368 34.0 30.5 35.0 2.0 36.0 92-93 29.373453065568036 34.0 30.0 35.0 2.0 35.5 94-95 29.00792892449274 34.0 30.0 35.0 2.0 35.0 96-97 28.616759324202228 34.0 29.0 35.0 2.0 35.0 98-99 28.084152366746963 34.0 29.0 35.0 2.0 35.0 100-101 25.91744930126917 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3930932892420742 1101 2 0.317545560916102 1101 3 0.362468204823287 1101 4 0.2692155739965827 1101 5 0.28786278669269905 1101 6 0.2174565849067136 1101 7 0.27363586387537 1101 8 0.21693519991248422 1101 9 0.21683437654806426 1101 10-11 0.27218749308660506 1101 12-13 0.3354323283734928 1101 14-15 0.5426206726103615 1101 16-17 0.5351734795803722 1101 18-19 0.5959683149250878 1101 20-21 0.5293071593466934 1101 22-23 0.49290382557560264 1101 24-25 0.5783430148455082 1101 26-27 0.6153406769618144 1101 28-29 0.5825451999335272 1101 30-31 0.528639522061475 1101 32-33 0.547606554837742 1101 34-35 0.5663559169409851 1101 36-37 0.5404977670261317 1101 38-39 0.5187435978136961 1101 40-41 0.6205455362121839 1101 42-43 0.58102812224422 1101 44-45 0.479034635678218 1101 46-47 0.5701690648727649 1101 48-49 0.3765498849455824 1101 50-51 0.3976498625393603 1101 52-53 0.5929408737446664 1101 54-55 0.5249565594166725 1101 56-57 0.5120307391445422 1101 58-59 0.45360567458293133 1101 60-61 0.5538707330170354 1101 62-63 0.48969695495522814 1101 64-65 0.544662774595686 1101 66-67 0.41258445637136276 1101 68-69 0.5069529588737325 1101 70-71 0.46305110826966 1101 72-73 0.441264614522062 1101 74-75 0.42144125659145715 1101 76-77 0.40840599262711663 1101 78-79 0.40021217043234003 1101 80-81 0.460383612955269 1101 82-83 0.4022316501065504 1101 84-85 0.46788904716023083 1101 86-87 0.551397917758333 1101 88-89 0.6089444970071263 1101 90-91 0.5860857329764677 1101 92-93 0.40260253692614256 1101 94-95 0.4801913440197936 1101 96-97 0.4808059621631031 1101 98-99 0.6150227721640036 1101 100-101 0.9222917178916141 1102 1 0.2798901551180748 1102 2 0.2992294206636785 1102 3 0.2545944766013193 1102 4 0.1839319740344223 1102 5 0.21474777668652223 1102 6 0.18358470942614957 1102 7 0.1865007907829792 1102 8 0.21294868803902745 1102 9 0.26928016959378454 1102 10-11 0.2869122426150952 1102 12-13 0.29502042369085046 1102 14-15 0.44294852223075054 1102 16-17 0.3757311304353266 1102 18-19 0.32231412863315256 1102 20-21 0.37434457616225103 1102 22-23 0.4071659182010521 1102 24-25 0.495407471328015 1102 26-27 0.36084799042864546 1102 28-29 0.4669018478983915 1102 30-31 0.4013066188854424 1102 32-33 0.5255288105107709 1102 34-35 0.3796211437961219 1102 36-37 0.487096221524844 1102 38-39 0.4205390025288409 1102 40-41 0.5047405845414659 1102 42-43 0.5410929312020656 1102 44-45 0.5219107498463984 1102 46-47 0.5173686826986099 1102 48-49 0.5004920493977849 1102 50-51 0.38813619923204357 1102 52-53 0.43816493866777506 1102 54-55 0.5739423869489642 1102 56-57 0.6876249137366202 1102 58-59 0.5640766756921494 1102 60-61 0.6904057789471594 1102 62-63 0.6977863959673911 1102 64-65 0.7480649806579365 1102 66-67 0.6154251856095314 1102 68-69 0.5938667704637837 1102 70-71 0.6839946429895392 1102 72-73 0.693235702032645 1102 74-75 0.8332409893020625 1102 76-77 0.7706801930709091 1102 78-79 0.8093978255846395 1102 80-81 0.7225500098488311 1102 82-83 0.6835553720135508 1102 84-85 0.7638251175411561 1102 86-87 0.8129869315196636 1102 88-89 0.7918986947663953 1102 90-91 0.634796502921052 1102 92-93 0.6456438441815848 1102 94-95 0.7390183145656408 1102 96-97 0.6488128806144751 1102 98-99 0.7429223838069987 1102 100-101 1.045599109674555 1103 1 0.25833619377966244 1103 2 0.3720458345486577 1103 3 0.2993672278634065 1103 4 0.2910815647428251 1103 5 0.2669478327389001 1103 6 0.2348486178787752 1103 7 0.22009291141935705 1103 8 0.2548263700909814 1103 9 0.35179728558945556 1103 10-11 0.37645956502184674 1103 12-13 0.16179191559420047 1103 14-15 0.1809192803574362 1103 16-17 0.4382826786094398 1103 18-19 0.5009803730870601 1103 20-21 0.4052523699913877 1103 22-23 0.3325412689834195 1103 24-25 0.514530069537976 1103 26-27 0.5143783806478694 1103 28-29 0.46248754070949616 1103 30-31 0.5031348729264948 1103 32-33 0.3914165674326924 1103 34-35 0.5284380381300977 1103 36-37 0.500230314113189 1103 38-39 0.47779978806295276 1103 40-41 0.4884064104223782 1103 42-43 0.6381272505477824 1103 44-45 0.5442021276067095 1103 46-47 0.5405070338121973 1103 48-49 0.46571482122455166 1103 50-51 0.3817964562722622 1103 52-53 0.41678932582231454 1103 54-55 0.42024274764968794 1103 56-57 0.5186550036752138 1103 58-59 0.6544695155461255 1103 60-61 0.6436151418790601 1103 62-63 0.6609592520547167 1103 64-65 0.7226889019225453 1103 66-67 0.7313713607623171 1103 68-69 0.6751296233845068 1103 70-71 0.6361670368266843 1103 72-73 0.5599533254115912 1103 74-75 0.5677317679570208 1103 76-77 0.41965687309551214 1103 78-79 0.5354785628554666 1103 80-81 0.6320512528286386 1103 82-83 0.6103495032562023 1103 84-85 0.6762902212926605 1103 86-87 0.7029120615502755 1103 88-89 0.7854508507812916 1103 90-91 0.7896390284813037 1103 92-93 0.7422410277312999 1103 94-95 0.759046486347998 1103 96-97 0.6195753750183322 1103 98-99 0.7018651048226374 1103 100-101 1.0416481445908197 1104 1 0.31594465169951036 1104 2 0.39172488140158634 1104 3 0.30938357578877884 1104 4 0.33353872094521364 1104 5 0.3327925311548938 1104 6 0.2576941948567466 1104 7 0.2573424563835687 1104 8 0.23112818022521253 1104 9 0.3708698651882827 1104 10-11 0.3900531800634255 1104 12-13 0.3288421344932928 1104 14-15 0.508419382300275 1104 16-17 0.4617766120833906 1104 18-19 0.520898522249837 1104 20-21 0.5260913410729842 1104 22-23 0.508918331472529 1104 24-25 0.4212953674520463 1104 26-27 0.443330890110019 1104 28-29 0.5337118557462546 1104 30-31 0.5895646120640166 1104 32-33 0.4694480696253933 1104 34-35 0.558727296319816 1104 36-37 0.5922346587319041 1104 38-39 0.5844338577325487 1104 40-41 0.5955561255384652 1104 42-43 0.5904087314251569 1104 44-45 0.4757625204017586 1104 46-47 0.4557877102678489 1104 48-49 0.3667613108690162 1104 50-51 0.4219522990801039 1104 52-53 0.43129264174503845 1104 54-55 0.48471399791380776 1104 56-57 0.5991005124441529 1104 58-59 0.5013424651047984 1104 60-61 0.5379827070020085 1104 62-63 0.5661246033497633 1104 64-65 0.5611936413380931 1104 66-67 0.5932065543981153 1104 68-69 0.49892530768049426 1104 70-71 0.5553130152741161 1104 72-73 0.6126844669119649 1104 74-75 0.5129250701267338 1104 76-77 0.5276499335680072 1104 78-79 0.5990768116627336 1104 80-81 0.5768249211770495 1104 82-83 0.7051893417787376 1104 84-85 0.7809291768048041 1104 86-87 0.7746386767883244 1104 88-89 0.6327664176391146 1104 90-91 0.6860439677048298 1104 92-93 0.8154556378091833 1104 94-95 0.7964991756002568 1104 96-97 0.6719569452347152 1104 98-99 0.7153033960381308 1104 100-101 0.8973359993055858 1105 1 0.2532439352496141 1105 2 0.20116948895836373 1105 3 0.26734549225441384 1105 4 0.221296417408972 1105 5 0.16879300333987146 1105 6 0.21041667419108734 1105 7 0.17690075155366003 1105 8 0.17885261186282264 1105 9 0.3323368470485093 1105 10-11 0.23397977648753 1105 12-13 0.21892556897308424 1105 14-15 0.17423992476817318 1105 16-17 0.3498267756833755 1105 18-19 0.34244627498230074 1105 20-21 0.32872785852718067 1105 22-23 0.25786045551794246 1105 24-25 0.2772853819505414 1105 26-27 0.2595835877693773 1105 28-29 0.29723874456182386 1105 30-31 0.13140368173854 1105 32-33 0.17090874055181615 1105 34-35 0.07311531735020793 1105 36-37 0.17057725919985245 1105 38-39 0.236083175637134 1105 40-41 0.41544706897010286 1105 42-43 0.23917537518883591 1105 44-45 0.06145955320347696 1105 46-47 0.13181168576266344 1105 48-49 0.17002475981703213 1105 50-51 0.010166138337439179 1105 52-53 0.044236790737564036 1105 54-55 0.10018516890227858 1105 56-57 0.11580367759690091 1105 58-59 0.21267853670741488 1105 60-61 0.07750965938094367 1105 62-63 -0.03783100520889349 1105 64-65 0.10371147691481042 1105 66-67 0.2013637768608021 1105 68-69 0.35831066510332477 1105 70-71 0.25144344426627185 1105 72-73 0.28382163059367116 1105 74-75 0.2468473230544319 1105 76-77 0.12022051160111857 1105 78-79 0.05721012219397181 1105 80-81 0.14637194141744203 1105 82-83 0.21764624733265947 1105 84-85 0.1651485127795489 1105 86-87 0.21230836826386934 1105 88-89 0.27264289283881027 1105 90-91 0.13838658028008766 1105 92-93 0.10358116939563544 1105 94-95 0.09837785130301313 1105 96-97 0.19053749968884048 1105 98-99 0.4456146446344391 1105 100-101 0.5934395652102005 1106 1 0.4100564099395996 1106 2 0.34988850303815155 1106 3 0.29684344687014885 1106 4 0.23958951479951907 1106 5 0.17654851841004415 1106 6 0.30368294176928146 1106 7 0.3376907878086115 1106 8 0.24703647529835848 1106 9 0.31300023510093666 1106 10-11 0.41448804945179063 1106 12-13 0.39508530616058835 1106 14-15 0.626315681052958 1106 16-17 0.5271453884408643 1106 18-19 0.5846037604760141 1106 20-21 0.5786850169891764 1106 22-23 0.6555232701160421 1106 24-25 0.6878074790893081 1106 26-27 0.7342793171142503 1106 28-29 0.5820399550387094 1106 30-31 0.5410585369054246 1106 32-33 0.6347862635354602 1106 34-35 0.5642181590605375 1106 36-37 0.6227266119814843 1106 38-39 0.6676666754700804 1106 40-41 0.6466709870896281 1106 42-43 0.6581504189159233 1106 44-45 0.8065588641966741 1106 46-47 0.6136197087621014 1106 48-49 0.7164414122031886 1106 50-51 0.776103908490434 1106 52-53 0.7838053549093971 1106 54-55 0.6490070505409236 1106 56-57 0.7073812944968623 1106 58-59 0.7356406437260574 1106 60-61 0.7196077411331245 1106 62-63 0.7183446383954646 1106 64-65 0.7016510622087182 1106 66-67 0.8434833990510953 1106 68-69 0.743015111145489 1106 70-71 0.897596393822397 1106 72-73 0.7560022744517241 1106 74-75 0.7958879598089297 1106 76-77 0.9541787968673603 1106 78-79 0.9655626185392805 1106 80-81 0.9496822520114137 1106 82-83 0.9604866843833655 1106 84-85 1.0017496901320264 1106 86-87 1.1450168773113312 1106 88-89 1.0817110822070255 1106 90-91 1.0780915432927607 1106 92-93 1.1256451598144501 1106 94-95 1.1358104656324741 1106 96-97 0.9669950214662215 1106 98-99 1.1106543699890672 1106 100-101 1.5003592634142997 1107 1 0.19338707269242406 1107 2 0.3157413071602235 1107 3 0.2755684490454797 1107 4 0.33951348475159904 1107 5 0.18099572454399748 1107 6 0.1991608089258321 1107 7 0.2755686968920159 1107 8 0.2557356718924524 1107 9 0.3188718762190561 1107 10-11 0.34580410400385375 1107 12-13 0.34280110724572666 1107 14-15 0.3640101350471241 1107 16-17 0.4043520339485198 1107 18-19 0.3590228339876944 1107 20-21 0.37678029126531243 1107 22-23 0.4294466563017991 1107 24-25 0.35580373124927434 1107 26-27 0.3702025242582607 1107 28-29 0.3324380972174126 1107 30-31 0.4460575877836206 1107 32-33 0.4824972876667246 1107 34-35 0.5779409307369363 1107 36-37 0.5310149905429213 1107 38-39 0.5621220738918566 1107 40-41 0.6392151776742949 1107 42-43 0.7586574029227791 1107 44-45 0.7721173402556047 1107 46-47 0.8404497271903679 1107 48-49 0.8514018311909481 1107 50-51 0.748788007402716 1107 52-53 0.6903815528402859 1107 54-55 0.839669196529151 1107 56-57 0.8241113655092249 1107 58-59 0.8620779614401286 1107 60-61 0.8914870549669587 1107 62-63 0.7499010577312717 1107 64-65 0.7943242180098835 1107 66-67 0.7632111402640689 1107 68-69 0.8708850761922307 1107 70-71 0.8058871545917938 1107 72-73 0.6977031258027111 1107 74-75 0.819885257131304 1107 76-77 0.6513646820594872 1107 78-79 0.7271676700238032 1107 80-81 0.808154336420813 1107 82-83 0.7940981718415401 1107 84-85 0.8032149110241384 1107 86-87 0.7946048054850046 1107 88-89 0.7820634233030965 1107 90-91 0.7638583772907275 1107 92-93 0.7920976785229321 1107 94-95 0.7865948264121059 1107 96-97 0.9093569982173761 1107 98-99 0.9543079924371902 1107 100-101 1.2907374376750695 1108 1 -0.017746497886811596 1108 2 0.0924295164369795 1108 3 0.0949488378075003 1108 4 0.03513829507215149 1108 5 0.017173094639787223 1108 6 0.06198783050895429 1108 7 0.0485379451892598 1108 8 -0.11953254461966623 1108 9 -0.058454651042545436 1108 10-11 -0.033298036113379226 1108 12-13 -0.10342818970895706 1108 14-15 0.19980405947670477 1108 16-17 0.043355530091666594 1108 18-19 0.20109048389575435 1108 20-21 0.13435147747349419 1108 22-23 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-1.7051223141967142 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5765.0 3 1424.0 4 1368.0 5 1401.0 6 1558.0 7 1786.0 8 2182.0 9 2750.0 10 3408.0 11 4690.0 12 6888.0 13 9456.0 14 10926.0 15 14881.0 16 20904.0 17 29990.0 18 25709.0 19 14543.0 20 12164.0 21 12003.0 22 12711.0 23 13375.0 24 15191.0 25 17344.0 26 20218.0 27 24217.0 28 29981.0 29 37232.0 30 47319.0 31 61943.0 32 83251.0 33 115617.0 34 167028.0 35 251082.0 36 393181.0 37 602596.0 38 577424.0 39 116671.0 40 60.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68241621588237 19.632404170487664 14.739554006168254 21.945625607461714 2 14.898252505479212 20.98872218211721 41.13765990517875 22.975365407224828 3 18.997310774421113 26.4558029659142 30.246906668159678 24.299979591505007 4 12.09294256687159 17.45477741997275 38.99709780365539 31.455182209500265 5 12.776567698742836 38.43279267612311 34.378839210607715 14.411800414526343 6 29.30495419633014 37.59279339695827 18.55658369264334 14.545668714068247 7 26.675370515849412 34.274025984350416 21.601614247235087 17.44898925256508 8 25.644534016250102 34.256554815921746 20.733552622070892 19.365358545757257 9 26.546812272806612 16.934826688869688 21.29679519772939 35.2215658405943 10-11 23.93130937107426 27.539289226486947 27.90038713323566 20.629014269203132 12-13 25.527718546254903 25.367793434489776 28.205420942837385 20.89906707641794 14-15 22.78455287123075 26.028311895848528 27.620022787244565 23.56711244567616 16-17 20.664516053266073 29.263512774950744 28.134251032773218 21.937720139009965 18-19 20.53887173932796 27.7010197189744 31.241125034479257 20.51898350721838 20-21 22.050539043760157 26.505788866354607 31.08968819066929 20.353983899215947 22-23 21.380215539133204 26.675297672157132 31.01559288433321 20.928893904376455 24-25 21.044614123230623 26.837858435399458 31.363598602919136 20.753928838450786 26-27 20.858753344389804 26.931999092753006 31.346413051643733 20.862834511213457 28-29 20.64777013524484 27.224044811310105 31.34953165029046 20.7786534031546 30-31 20.920582405444346 26.876292563519684 31.251917458674534 20.951207572361437 32-33 20.742293813092644 26.883028331378316 31.20628297285897 21.16839488267007 34-35 21.22154132504941 27.012371350700725 31.009451225127382 20.75663609912248 36-37 21.1921824915938 26.71261929436136 31.131334264014892 20.96386395002995 38-39 20.815464710006893 27.16134877054783 31.136268383186106 20.886918136259176 40-41 21.025076908128874 27.05995854166945 31.0644366770776 20.850527873124072 42-43 20.815984437281752 27.127719899966333 30.720458357592896 21.335837305159014 44-45 21.045047595814147 27.298095860463377 30.482845120438167 21.17401142328431 46-47 21.390484206938105 27.489043717341723 29.959161181980363 21.161310893739813 48-49 21.23950467482097 27.57736296039493 29.845436587470974 21.337695777313122 50-51 21.195330839893852 27.855330922940297 29.65140892810687 21.297929309058976 52-53 21.350466279780868 28.093300373229656 29.301697074595197 21.254536272394276 54-55 21.216592188333998 27.949056676000833 29.34493856711248 21.489412568552687 56-57 21.264447207256985 28.20289878625853 29.065510258070166 21.467143748414315 58-59 21.4737101388452 27.908162730919578 29.28964409067285 21.328483039562375 60-61 21.30525308341008 27.952146197879983 29.570832656156888 21.171768062553046 62-63 21.05504010587025 27.8692636758706 29.755872129309648 21.319824088949506 64-65 21.104188047953787 28.007764792720984 29.440344132493323 21.447703026831906 66-67 21.136970559887136 28.027134813167788 29.075635320911626 21.760259306033454 68-69 21.16862029013981 28.00523101103988 29.3017156444935 21.524433054326817 70-71 21.352276161477647 27.881818185100578 29.13949213549841 21.626413517923364 72-73 21.214132139949417 27.827574317213145 29.221941607710537 21.736351935126898 74-75 21.046136903203678 28.38752832215492 28.823116294604574 21.74321848003683 76-77 21.18826819707664 28.179749742290426 28.897869727245855 21.73411233338708 78-79 21.228898538711107 28.17991289876626 28.858392320354838 21.732796242167794 80-81 21.208897440091924 28.202142061916053 28.71089891994117 21.878061578050854 82-83 21.320947233263325 28.190645773550354 28.868365782435408 21.620041210750912 84-85 21.316498418046997 28.170357890341645 28.65611381176177 21.85702987984959 86-87 21.11218389110669 28.281912134622694 28.802391035171294 21.803512939099328 88-89 21.376813623152955 28.14947981424944 28.560061028095067 21.913645534502532 90-91 21.236757457280095 28.088557103056015 28.807443798487064 21.867241641176825 92-93 21.34735286892944 28.173595156559305 28.713298510266306 21.76575346424495 94-95 21.22775439132041 28.095364965712072 28.618412793601394 22.058467849366128 96-97 21.198073769470135 28.081974267068848 28.741537335438906 21.978414628022108 98-99 21.15147274758325 28.109524296549488 28.43429384262383 22.304709113243433 100-101 21.23706992503592 27.958161197343735 28.58569264438463 22.21907623323571 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1052.0 1 811.5 2 714.0 3 860.0 4 758.5 5 786.5 6 1379.5 7 2993.5 8 5220.5 9 6116.5 10 5170.5 11 4248.0 12 4244.5 13 4454.0 14 4617.0 15 4797.5 16 5034.0 17 5391.5 18 5731.5 19 6225.5 20 7007.5 21 7999.5 22 9437.5 23 11629.0 24 13918.5 25 16820.0 26 21502.5 27 26999.0 28 30721.0 29 34669.5 30 40484.5 31 46170.0 32 53215.0 33 60832.5 34 68188.0 35 74741.5 36 83130.5 37 90329.5 38 90985.5 39 90577.0 40 90537.0 41 91482.5 42 92092.0 43 92462.5 44 94522.5 45 97005.0 46 98192.5 47 100655.0 48 108951.0 49 107445.5 50 95555.0 51 87790.5 52 83910.0 53 80781.5 54 76208.0 55 69419.0 56 61605.0 57 54787.5 58 49776.5 59 44180.0 60 36634.5 61 29287.0 62 22336.0 63 16313.0 64 11228.0 65 7680.0 66 5547.0 67 4156.5 68 3165.5 69 2353.0 70 1616.0 71 990.0 72 662.5 73 485.5 74 313.0 75 208.0 76 152.0 77 90.0 78 51.0 79 31.5 80 28.0 81 22.5 82 17.0 83 14.0 84 10.5 85 10.0 86 8.0 87 6.0 88 5.0 89 4.0 90 3.0 91 1.5 92 2.0 93 2.5 94 1.5 95 1.5 96 1.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05573530351374269 2 0.09035328024280954 3 0.06432662620562789 4 0.12154194749402307 5 0.09894460293469476 6 0.09616505735790837 7 0.10392612617620801 8 0.09028108425380212 9 0.10995449125832915 10-11 0.10264464737132599 12-13 0.08587712892434834 14-15 0.08881911547640148 16-17 0.10141731555819954 18-19 0.08188830053168736 20-21 0.0672325147631773 22-23 0.06941644343065231 24-25 0.05800947716747701 26-27 0.05129525018978521 28-29 0.06279246143921982 30-31 0.045934698005982884 32-33 0.046674706893309124 34-35 0.0425234375253814 36-37 0.04687324586307959 38-39 0.04694544185208702 40-41 0.06042804279922621 42-43 0.05663775337633567 44-45 0.04488785616537502 46-47 0.03376967385822946 48-49 0.0414043996957661 50-51 0.02535884113886285 52-53 0.0371989833360828 54-55 0.04328149540995951 56-57 0.04763130374765769 58-59 0.05306405192046745 60-61 0.050067918376658756 62-63 0.05649336139832079 64-65 0.03866095211348343 66-67 0.05835240811526234 68-69 0.04927376249757692 70-71 0.023102716482380388 72-73 0.018843153130941506 74-75 0.027506671811834152 76-77 0.04084488078095845 78-79 0.0584607020987735 80-81 0.037397522305853255 82-83 0.055663107524735245 84-85 0.06012120984594459 86-87 0.062413432496930765 88-89 0.08416247418542168 90-91 0.059579739928388796 92-93 0.07064377524377878 94-95 0.07705116926818897 96-97 0.08461369911671816 98-99 0.1154413864228945 100-101 0.10991839326382545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2770237.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.4128343167132 #Duplication Level Percentage of deduplicated Percentage of total 1 71.90684167179683 35.53120853766625 2 15.239023986511203 15.060067347877926 3 5.612128780791714 8.319335688279558 4 2.491012027341909 4.923518583519424 5 1.357426859017566 3.35371542408457 6 0.8096666942775486 2.400475572965843 7 0.5252241157135298 1.8166968546226396 8 0.3255021671631239 1.286718772461002 9 0.2563215097000246 1.13990150615554 >10 1.284395236468163 11.689373778256769 >50 0.11610816488689549 3.973586168452902 >100 0.07107996752709068 6.526427646215054 >500 0.0035857239086074526 1.172032789296879 >1k 0.0014635607790084997 1.2593937623203493 >5k 7.317803895042498E-5 0.3367881220319772 >10k+ 1.4635607790084996E-4 1.2107594457932924 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19503 0.7040191868060386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13981 0.5046860611565003 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9314 0.3362167208076421 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4364 0.15753164801423125 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3360 0.12128926153249704 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3071 0.11085694112092215 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.6097994503719356E-5 0.0 12-13 0.0 0.0 0.0 3.6097994503719356E-5 0.0 14-15 0.0 0.0 0.0 9.024498625929839E-5 0.0 16-17 0.0 0.0 0.0 1.9853896977045646E-4 0.0 18-19 0.0 0.0 0.0 2.526859615260355E-4 0.0 20-21 0.0 0.0 0.0 3.068329532816145E-4 0.0 22-23 0.0 0.0 0.0 5.234209203039306E-4 0.0 24-25 0.0 0.0 0.0 6.858618955706678E-4 0.0 26-27 0.0 0.0 0.0 9.565968543485629E-4 0.0 28-29 0.0 0.0 0.0 0.0019853896977045644 0.0 30-31 0.0 0.0 0.0 0.0051078662222762885 0.0 32-33 0.0 0.0 0.0 0.010378173419819316 0.0 34-35 0.0 0.0 0.0 0.018590467169415467 0.0 36-37 0.0 0.0 0.0 0.03025011939411682 0.0 38-39 0.0 0.0 0.0 0.04618738396750892 0.0 40-41 0.0 0.0 0.0 0.06470565514791694 0.0 42-43 0.0 0.0 0.0 0.08454150312771073 0.0 44-45 0.0 0.0 0.0 0.11125401906046306 0.0 46-47 0.0 0.0 0.0 0.14320074419625467 0.0 48-49 0.0 0.0 0.0 0.17740359398852878 0.0 50-51 0.0 0.0 0.0 0.21730992691239054 0.0 52-53 0.0 0.0 0.0 0.2601582463883054 0.0 54-55 0.0 0.0 0.0 0.31040665473748275 0.0 56-57 0.0 0.0 0.0 0.3721883723305984 0.0 58-59 0.0 0.0 0.0 0.44070236589865774 0.0 60-61 0.0 0.0 0.0 0.5196125818837883 0.0 62-63 0.0 0.0 0.0 0.6002374526078454 0.0 64-65 0.0 0.0 0.0 0.6854648176311269 0.0 66-67 0.0 0.0 0.0 0.7927119593016771 0.0 68-69 0.0 0.0 0.0 0.9143080537874557 0.0 70-71 0.0 0.0 0.0 1.0426183752509262 0.0 72-73 0.0 0.0 0.0 1.1823717609720756 0.0 74-75 1.8048997251859678E-5 0.0 0.0 1.3325394181075483 0.0 76-77 3.6097994503719356E-5 0.0 0.0 1.4953233243220705 0.0 78-79 3.6097994503719356E-5 0.0 0.0 1.6830148467441595 0.0 80-81 3.6097994503719356E-5 0.0 0.0 1.8907407561158125 0.0 82-83 3.6097994503719356E-5 0.0 0.0 2.103989658646535 0.0 84-85 3.6097994503719356E-5 0.0 0.0 2.327382097632802 0.0 86-87 3.6097994503719356E-5 0.0 0.0 2.5753031238843462 0.0 88-89 5.4146991755579034E-5 0.0 0.0 2.84340292906347 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 21175 0.0 30.948565 1 GGTATCA 14230 0.0 30.123215 1 ATCAACG 25080 0.0 26.015657 3 TCAACGC 25265 0.0 25.825163 4 TATCAAC 25465 0.0 25.734766 2 TACATGG 15910 0.0 25.657991 2 GTACATG 16335 0.0 25.484999 1 CAACGCA 25720 0.0 25.368761 5 AACGCAG 26060 0.0 25.12805 6 ACATGGG 16055 0.0 24.212229 3 GAGTACT 13635 0.0 23.276567 12-13 ACGCAGA 28875 0.0 22.513762 7 CGCAGAG 28885 0.0 22.340643 8 GTATAGG 935 0.0 21.855288 1 GTACTTT 14555 0.0 21.576988 14-15 CATGGGG 5825 0.0 21.37456 4 CATGGGA 9515 0.0 21.226185 4 AGTACTT 14290 0.0 21.145721 12-13 GCAGAGT 30150 0.0 20.930471 9 GTGTAGC 1955 0.0 20.661974 1 >>END_MODULE