FastQCFastQC Report
Thu 26 May 2016
SRR936763_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936763_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2770237
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181950.6568030099951737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148780.5370659622263365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103000.3718093433883094No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58090.20969325007210576No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38250.13807482897672654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27940.10085779664339188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA166650.028.393791
GTATCAA237400.027.9366261
TATCAAC288550.022.9313032
ATCAACG288400.022.8929923
TCAACGC290000.022.7658654
GAGTACT157900.022.66587312-13
CAACGCA292800.022.5806035
AACGCAG298650.022.1382886
TACATGG153950.021.5056112
GTACATG155700.021.2673551
GTACTTT168600.021.14289314-15
AGTACTT164000.021.01187712-13
ACATGGG155200.020.3216723
AGAGTAC258350.019.89271410-11
ACGCAGA331650.019.7779397
CGCAGAG332900.019.5613528
GCAGAGT344400.018.6461329
CATGGGA91350.018.3541164
ACTTTTT195100.018.30778516-17
CGTATAG2603.2782373E-718.2724151
Produced by FastQC (version 0.11.5)