FastQCFastQC Report
Thu 26 May 2016
SRR936762_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936762_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732324
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190890.6986360329155693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139010.508761040052351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91900.3363437132638735No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44720.1636701943107772No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33790.12366761774957875No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30680.1122853658643704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA215350.029.837441
GGTATCA145850.029.3594591
GTACATG163550.025.3393611
TACATGG160600.025.1833652
ATCAACG257200.024.7783053
TCAACGC259050.024.5472164
TATCAAC259700.024.5406782
CAACGCA259950.024.4644685
AACGCAG264750.024.0537416
ACATGGG160850.024.0501143
GAGTACT137650.023.40537512-13
CATGGGA93950.022.0556284
GTACTTT147350.021.81583214-15
ACGCAGA294950.021.4780867
CGCAGAG295000.021.4265068
AGTACTT144850.021.01177412-13
ATGGGAT38450.020.8910315
AGAGTAC235450.020.12270710-11
GCACCGT2601.4837497E-820.1063446
GCAGAGT306900.020.100169
Produced by FastQC (version 0.11.5)