FastQCFastQC Report
Thu 26 May 2016
SRR936762_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936762_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2732324
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177990.6514234768643836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144230.5278656557567843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99710.3649274390592038No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT59290.2169947634321552No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38080.1393685375526475No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA171550.029.1401331
GTATCAA242450.027.713651
TATCAAC292850.022.7418732
ATCAACG294350.022.607773
TCAACGC295400.022.5253494
GAGTACT160500.022.43045412-13
CAACGCA298250.022.3101025
AACGCAG303450.021.9261886
GTACATG155650.021.4926531
TACATGG153300.021.4121022
AGTACTT165400.021.34958512-13
GTACTTT168500.021.18403814-15
CGATTCG1604.9997332E-520.7773519
ACATGGG155800.020.3044133
AGAGTAC259600.019.98613710-11
ACGCAGA337050.019.6276787
CGCAGAG338550.019.4284928
GCAGAGT347650.018.6194029
GTATAGG8600.018.2340031
ACTTTTT197150.018.12981416-17
Produced by FastQC (version 0.11.5)