##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936762_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2732324 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.670933974155336 31.0 31.0 34.0 30.0 34.0 2 31.74805257356009 31.0 31.0 34.0 30.0 34.0 3 31.875103025849057 31.0 31.0 34.0 30.0 34.0 4 35.67183284266434 37.0 35.0 37.0 33.0 37.0 5 35.55826578399926 37.0 35.0 37.0 33.0 37.0 6 35.60509954163562 37.0 35.0 37.0 33.0 37.0 7 35.54485558813669 37.0 35.0 37.0 33.0 37.0 8 35.52630910536232 37.0 35.0 37.0 33.0 37.0 9 37.10428704648497 39.0 37.0 39.0 33.0 39.0 10-11 36.97187449219053 39.0 37.0 39.0 33.0 39.0 12-13 36.87387165650925 39.0 37.0 39.0 33.0 39.0 14-15 37.88594215034527 40.0 37.0 41.0 33.0 41.0 16-17 37.78133651060416 39.0 37.0 41.0 33.0 41.0 18-19 37.86255052475475 39.5 37.0 41.0 32.5 41.0 20-21 37.99706275683265 40.0 37.0 41.0 33.0 41.0 22-23 37.913502571437355 40.0 37.0 41.0 33.0 41.0 24-25 37.79919328747249 40.0 37.0 41.0 32.0 41.0 26-27 37.56847302882089 39.0 36.5 41.0 32.0 41.0 28-29 37.3793821669758 39.0 36.0 41.0 32.0 41.0 30-31 37.26987575411994 39.0 36.0 40.0 32.0 41.0 32-33 37.124063068655104 39.0 36.0 40.5 31.0 41.0 34-35 36.96073214596805 39.0 35.0 40.0 31.0 41.0 36-37 36.733779193097156 39.0 35.0 40.0 30.5 41.0 38-39 36.54131556140487 39.0 35.0 40.0 30.0 41.0 40-41 36.26540373689211 38.5 35.0 40.0 30.0 41.0 42-43 35.95957068049031 38.0 35.0 40.0 29.5 41.0 44-45 35.62826004529478 38.0 35.0 40.0 28.5 41.0 46-47 35.177030798690055 38.0 34.5 40.0 26.5 41.0 48-49 34.70368338454737 38.0 34.0 40.0 24.5 41.0 50-51 34.41539052469619 38.0 33.5 40.0 24.0 41.0 52-53 34.160381601889085 38.0 33.0 40.0 23.5 41.0 54-55 33.85148942804733 38.0 33.0 40.0 23.0 41.0 56-57 33.55760334425932 37.5 33.0 40.0 22.0 41.0 58-59 33.16318159925397 37.0 32.0 39.5 20.5 41.0 60-61 32.77591676536165 36.5 31.5 39.0 19.0 40.0 62-63 32.40701615181801 36.0 31.0 39.0 18.0 40.0 64-65 31.94788850077809 35.5 31.0 39.0 15.5 40.0 66-67 31.45354028292399 35.0 30.0 38.0 13.0 40.0 68-69 30.946176405140825 35.0 29.5 38.0 8.0 40.0 70-71 30.493034684027222 34.0 29.0 37.0 8.0 39.0 72-73 30.028966184098223 34.0 29.0 37.0 7.0 39.0 74-75 29.482244236042284 34.0 28.0 36.0 4.0 39.0 76-77 28.18777879197343 31.5 26.5 34.5 2.0 37.0 78-79 28.891096919691808 33.5 28.0 35.0 2.0 37.5 80-81 28.596832037488966 33.5 27.5 35.0 2.0 37.0 82-83 28.28110081381271 33.0 27.0 35.0 2.0 36.5 84-85 27.834439107514335 33.0 26.0 35.0 2.0 36.0 86-87 27.350106722336005 33.0 26.0 35.0 2.0 36.0 88-89 26.82406661874653 32.0 25.0 34.5 2.0 35.0 90-91 26.201977510719814 32.0 24.5 34.0 2.0 35.0 92-93 25.57323161528428 31.5 22.0 34.0 2.0 35.0 94-95 24.841958164551492 31.0 19.0 34.0 2.0 35.0 96-97 23.90322103088799 31.0 8.5 34.0 2.0 35.0 98-99 22.768510981860132 30.5 2.0 34.0 2.0 35.0 100-101 19.39135000095157 26.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3556151148603419 1101 2 0.2806788707895329 1101 3 0.23395583003014409 1101 4 0.15088357693853993 1101 5 0.26050066345359113 1101 6 0.3158428154899866 1101 7 0.22129591601661502 1101 8 0.2799802325213321 1101 9 0.3574108653321275 1101 10-11 0.3523560085620687 1101 12-13 0.24843842744007105 1101 14-15 0.44841894713177055 1101 16-17 0.3646946692495945 1101 18-19 0.4541915132386265 1101 20-21 0.3445432471278025 1101 22-23 0.4577848549780228 1101 24-25 0.409878005521378 1101 26-27 0.4416928852975417 1101 28-29 0.4137818449737196 1101 30-31 0.3155000625542854 1101 32-33 0.5151930074094153 1101 34-35 0.5149817596695101 1101 36-37 0.5556393949007798 1101 38-39 0.5574271413950669 1101 40-41 0.5768724810422228 1101 42-43 0.644405872855458 1101 44-45 0.5435332000865003 1101 46-47 0.4762564805954028 1101 48-49 0.5767811905098554 1101 50-51 0.4628732995528466 1101 52-53 0.6000704478754031 1101 54-55 0.6091021471172766 1101 56-57 0.5745724425539365 1101 58-59 0.7833256620157556 1101 60-61 0.7381643993732538 1101 62-63 0.6887136888817054 1101 64-65 0.8040978044960134 1101 66-67 0.7393068456329779 1101 68-69 0.6748752893114975 1101 70-71 0.7024738748443049 1101 72-73 0.6982124388632336 1101 74-75 0.6794419052360503 1101 76-77 0.6414497116334488 1101 78-79 0.9246906606404082 1101 80-81 1.276691312055295 1101 82-83 1.0602724555999608 1101 84-85 0.8862319972511443 1101 86-87 0.8561255455002836 1101 88-89 0.7006039008142899 1101 90-91 0.39446272920660164 1101 92-93 0.8672281330321105 1101 94-95 1.0096154821373524 1101 96-97 0.9768063712213824 1101 98-99 1.2284825135377666 1101 100-101 1.2523236405617375 1102 1 0.37521876290705336 1102 2 0.32499051103735965 1102 3 0.2777877294038795 1102 4 0.22113928273312666 1102 5 0.2732869303567682 1102 6 0.2887024835697787 1102 7 0.33094108366105246 1102 8 0.2548698504618301 1102 9 0.35569679973908563 1102 10-11 0.3545545466073037 1102 12-13 0.3630796786750352 1102 14-15 0.41738128634169414 1102 16-17 0.45801260095338137 1102 18-19 0.5536230552753523 1102 20-21 0.40777153085586093 1102 22-23 0.44915239933528284 1102 24-25 0.40190282779147424 1102 26-27 0.4100537997881588 1102 28-29 0.4465613436630278 1102 30-31 0.5398561179199177 1102 32-33 0.5553901804972199 1102 34-35 0.5230738382363356 1102 36-37 0.6142612816381146 1102 38-39 0.5079623497362178 1102 40-41 0.6093905086118951 1102 42-43 0.5729429974857396 1102 44-45 0.6771770860904454 1102 46-47 0.6872321474264709 1102 48-49 0.7234775461146938 1102 50-51 0.7286784418524874 1102 52-53 0.5858620846054166 1102 54-55 0.5771371484518895 1102 56-57 0.8278650808870154 1102 58-59 0.7431621242765374 1102 60-61 0.8719846395214219 1102 62-63 0.7289396114494338 1102 64-65 0.7507145461564839 1102 66-67 0.8662218104315791 1102 68-69 0.7501234271526513 1102 70-71 0.7569971863523755 1102 72-73 0.8271242324759136 1102 74-75 0.7793162098355033 1102 76-77 0.8235075160886396 1102 78-79 0.8698250811738113 1102 80-81 0.8357826536274011 1102 82-83 0.8972749905943509 1102 84-85 0.7897989899681157 1102 86-87 0.787437946400722 1102 88-89 0.8410249495480002 1102 90-91 0.7660011555351609 1102 92-93 1.0660811367870302 1102 94-95 1.232516268659353 1102 96-97 1.1960582367231858 1102 98-99 1.4521113973533524 1102 100-101 1.4946566068549032 1103 1 0.2010393232736689 1103 2 0.20884287966893922 1103 3 0.24847416975900316 1103 4 0.24466910027884836 1103 5 0.28661182788646045 1103 6 0.3077379190930287 1103 7 0.3442184139660114 1103 8 0.23809138708910638 1103 9 0.24590680594753422 1103 10-11 0.17625729881397945 1103 12-13 0.21341717946010164 1103 14-15 0.400121590916072 1103 16-17 0.35662625315871566 1103 18-19 0.43185816709436864 1103 20-21 0.4035483952697021 1103 22-23 0.43182428741433654 1103 24-25 0.4145536503852867 1103 26-27 0.42885520868404825 1103 28-29 0.3931930281708347 1103 30-31 0.41305764954337576 1103 32-33 0.25536526433187845 1103 34-35 0.39963647819671877 1103 36-37 0.3690786471757619 1103 38-39 0.37083592490736805 1103 40-41 0.43315754452515165 1103 42-43 0.4105048374518887 1103 44-45 0.4811215106866129 1103 46-47 0.43675827696787906 1103 48-49 0.3715950371597998 1103 50-51 0.2813439567471008 1103 52-53 0.3276587975248546 1103 54-55 0.3044884571168396 1103 56-57 0.3429346247873468 1103 58-59 0.3982054856113848 1103 60-61 0.4486919888635583 1103 62-63 0.5096411662387368 1103 64-65 0.4676980530964592 1103 66-67 0.35425875636864745 1103 68-69 0.4063374364210546 1103 70-71 0.5497862730719554 1103 72-73 0.6720835480844514 1103 74-75 0.5483656478318331 1103 76-77 0.5639336854753196 1103 78-79 0.7363638036746529 1103 80-81 0.9793964963075545 1103 82-83 0.8362126250206678 1103 84-85 0.7711119174425107 1103 86-87 0.7119687348532153 1103 88-89 0.6643485394938118 1103 90-91 0.5968909200362127 1103 92-93 0.8118123387243124 1103 94-95 1.0811093804721885 1103 96-97 1.222471791528971 1103 98-99 1.3950322205752208 1103 100-101 1.3389764933525043 1104 1 0.2447211706117045 1104 2 0.20188564212350357 1104 3 0.2028947384996478 1104 4 0.1927963185811734 1104 5 0.12229709622086204 1104 6 0.19686513739535627 1104 7 0.20384638061129579 1104 8 0.2551202517459359 1104 9 0.32096325238755696 1104 10-11 0.27594834007975066 1104 12-13 0.32702216128605954 1104 14-15 0.35580450731547586 1104 16-17 0.3596351263680404 1104 18-19 0.2744826058371501 1104 20-21 0.32535927831438727 1104 22-23 0.30730140950114304 1104 24-25 0.44015777696215963 1104 26-27 0.4753181122547119 1104 28-29 0.3945921087780633 1104 30-31 0.4919813185619759 1104 32-33 0.4163273126534577 1104 34-35 0.3158443037258962 1104 36-37 0.4324431971009304 1104 38-39 0.5368981442787657 1104 40-41 0.5060965011212559 1104 42-43 0.5413820023011553 1104 44-45 0.6450823830406875 1104 46-47 0.5265561174639259 1104 48-49 0.6229232389985881 1104 50-51 0.6760369223233269 1104 52-53 0.6750790455470934 1104 54-55 0.6454306208970451 1104 56-57 0.5853110412936573 1104 58-59 0.574895671628056 1104 60-61 0.5926563891201369 1104 62-63 0.8239222224713671 1104 64-65 0.8967394257712478 1104 66-67 0.6692876210254788 1104 68-69 0.7055503934447884 1104 70-71 0.7052879835701411 1104 72-73 0.5915062549478236 1104 74-75 0.7298654340195618 1104 76-77 0.784272898646158 1104 78-79 1.0694040003819438 1104 80-81 1.1216031458692406 1104 82-83 1.0347897043450232 1104 84-85 0.9595349450242914 1104 86-87 0.9939374113552475 1104 88-89 0.8796760998957787 1104 90-91 0.9255963936303964 1104 92-93 1.0288770169368426 1104 94-95 1.0877904794672695 1104 96-97 1.3208795315332473 1104 98-99 1.5991536659461865 1104 100-101 1.485125306694389 1105 1 0.25295009035433935 1105 2 0.2779227794979242 1105 3 0.23531656940607704 1105 4 0.27472464006215347 1105 5 0.2412721498386574 1105 6 0.33068204675306845 1105 7 0.2708223372655425 1105 8 0.22914124734363384 1105 9 0.1909696865223367 1105 10-11 0.2853924082589998 1105 12-13 0.350202712138298 1105 14-15 0.4385629450624009 1105 16-17 0.48988600118460823 1105 18-19 0.45297888081174875 1105 20-21 0.4347128735282979 1105 22-23 0.16167148513044793 1105 24-25 0.3468590412132002 1105 26-27 0.3309373695072253 1105 28-29 0.31440083436785216 1105 30-31 0.3528638109426083 1105 32-33 0.23649251188587783 1105 34-35 0.26855138127415046 1105 36-37 0.18872471871735996 1105 38-39 0.4823106061819118 1105 40-41 0.5402054863340382 1105 42-43 0.5587330012852334 1105 44-45 0.5895608794849778 1105 46-47 0.6544812218775178 1105 48-49 0.6019610536503563 1105 50-51 0.5519435709292608 1105 52-53 0.5857617975507097 1105 54-55 0.4513008658456883 1105 56-57 0.4918375679896698 1105 58-59 0.783473389203408 1105 60-61 0.6922115872237598 1105 62-63 0.7835341989924203 1105 64-65 0.8186295375220105 1105 66-67 0.743378376189721 1105 68-69 0.7694037628995787 1105 70-71 0.7451502140701969 1105 72-73 0.8308526823098283 1105 74-75 0.5880219415260477 1105 76-77 0.5251549959483377 1105 78-79 0.699876627089246 1105 80-81 0.9727450919128131 1105 82-83 0.9542315149331237 1105 84-85 0.66378469104529 1105 86-87 0.634848067185473 1105 88-89 0.6904528241310182 1105 90-91 0.7004503839227532 1105 92-93 0.8816063881977065 1105 94-95 1.0200816522858709 1105 96-97 0.9457016333506054 1105 98-99 1.2028852383584194 1105 100-101 1.1718229023598141 1106 1 0.18191439563763012 1106 2 0.20607425832352888 1106 3 0.14179610962535705 1106 4 0.1512872737603459 1106 5 0.13139952737653005 1106 6 0.25548986374631966 1106 7 0.10401229834375414 1106 8 0.17548112281403405 1106 9 0.22782429628662726 1106 10-11 0.2207420951206558 1106 12-13 0.1905694017515529 1106 14-15 0.41629730602500814 1106 16-17 0.40238688348291163 1106 18-19 0.3510845761102175 1106 20-21 0.21987402655727806 1106 22-23 0.3387652302178168 1106 24-25 0.3125666374299527 1106 26-27 0.3936048964464405 1106 28-29 0.359896348897891 1106 30-31 0.1646277063946755 1106 32-33 0.2271319560455538 1106 34-35 0.1900109127822418 1106 36-37 0.39752419721614984 1106 38-39 0.420666896439144 1106 40-41 0.408762879736301 1106 42-43 0.3255117983606013 1106 44-45 0.3290246574962765 1106 46-47 0.3878325894077008 1106 48-49 0.2974682326614513 1106 50-51 0.12943212914438362 1106 52-53 0.03427578480276594 1106 54-55 0.24678037317265478 1106 56-57 0.25613123947317007 1106 58-59 0.37210617975111404 1106 60-61 0.3882860123486154 1106 62-63 0.523156293216843 1106 64-65 0.4098785547031589 1106 66-67 0.44028312742022635 1106 68-69 0.6460547142190691 1106 70-71 0.6213900114535384 1106 72-73 0.6286614571600602 1106 74-75 0.5464040329364828 1106 76-77 0.41112904566770325 1106 78-79 0.429723968120161 1106 80-81 0.8949568156468679 1106 82-83 0.9073136126293839 1106 84-85 0.6502226394591339 1106 86-87 0.6538956438705128 1106 88-89 0.33822558716390105 1106 90-91 0.7180035257884789 1106 92-93 1.0104116983517493 1106 94-95 0.8721340098279136 1106 96-97 0.990405859451208 1106 98-99 1.2519705803372432 1106 100-101 1.2415919503169235 1107 1 0.1402384119910174 1107 2 0.16451782603155252 1107 3 0.14800968102838752 1107 4 0.0972675829489944 1107 5 0.11252698127833582 1107 6 0.13171571210799726 1107 7 0.1200532771630165 1107 8 0.13863749312524476 1107 9 0.1877563558358375 1107 10-11 0.16497132858549435 1107 12-13 0.2977060693320297 1107 14-15 0.2535746222580073 1107 16-17 0.2927098390117209 1107 18-19 0.2377067437681859 1107 20-21 0.2086983587741642 1107 22-23 0.23354853593792768 1107 24-25 0.3699221447782506 1107 26-27 0.40563995133517494 1107 28-29 0.3774829133757649 1107 30-31 0.3322571806309398 1107 32-33 0.4277123701247163 1107 34-35 0.41298223476037776 1107 36-37 0.3717420476756459 1107 38-39 0.4397946960028989 1107 40-41 0.6249987999718272 1107 42-43 0.35457165747356356 1107 44-45 0.2364616933855217 1107 46-47 0.41621703700415935 1107 48-49 0.5182450780790546 1107 50-51 0.41254266944976337 1107 52-53 0.43186640186893754 1107 54-55 0.3273674025062334 1107 56-57 0.2827305815235377 1107 58-59 0.4573094647315088 1107 60-61 0.38281156153392715 1107 62-63 0.5642957856897581 1107 64-65 0.5201704602540111 1107 66-67 0.42790831068065316 1107 68-69 0.4719986693068563 1107 70-71 0.28159258127128695 1107 72-73 0.2616786687409274 1107 74-75 0.39579072137588156 1107 76-77 0.40603726646224914 1107 78-79 0.6387258394624027 1107 80-81 0.8007353297772859 1107 82-83 0.7719736123909975 1107 84-85 0.7187935657139484 1107 86-87 0.6905178711253619 1107 88-89 0.48741173207968913 1107 90-91 0.34191248558441956 1107 92-93 0.3978827640632794 1107 94-95 0.6524169162488782 1107 96-97 0.6135461981999129 1107 98-99 0.5691421716933114 1107 100-101 0.600780479108181 1108 1 0.12504557117588178 1108 2 0.16024906478453005 1108 3 0.127283549030917 1108 4 0.12662753625865264 1108 5 0.040609272995666856 1108 6 0.16148761036197357 1108 7 0.12534144408096637 1108 8 0.1876336508996772 1108 9 0.13399146113226834 1108 10-11 0.15641143363875187 1108 12-13 0.06608868690900493 1108 14-15 0.17420883265633336 1108 16-17 0.16231613247993693 1108 18-19 0.20635637170456533 1108 20-21 0.263060500693328 1108 22-23 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-1.265204818887149 2316 98-99 -1.524878338393389 2316 100-101 -1.7252701367555687 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 17.0 4 33.0 5 78.0 6 198.0 7 527.0 8 1220.0 9 2403.0 10 3928.0 11 6358.0 12 9211.0 13 13061.0 14 16869.0 15 22934.0 16 29992.0 17 38988.0 18 34165.0 19 23121.0 20 20927.0 21 22278.0 22 23518.0 23 26682.0 24 30761.0 25 36242.0 26 44363.0 27 53940.0 28 65906.0 29 80187.0 30 96088.0 31 117314.0 32 143947.0 33 179129.0 34 227965.0 35 300688.0 36 400713.0 37 447863.0 38 202827.0 39 7881.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.6546351220555 21.265120576576997 15.843823717615498 21.236420583751997 2 15.747528296665891 22.76964735129152 39.24221594627477 22.24060840576782 3 19.64750359886286 26.737071270478197 30.88949834798195 22.725926782676996 4 13.136180043069562 18.74741062919332 37.96581225359804 30.150597074139085 5 12.72766734462549 38.965039797293116 34.26197953972245 14.045313318358946 6 29.295940012970647 38.57108454195037 17.721068218849595 14.411907226229392 7 26.38947650425059 34.64816032066475 21.418982521838554 17.543380653246103 8 25.910727879604146 35.06038824149441 20.164475610470223 18.864408268431223 9 26.448656894277544 16.9134773182097 21.019688733839764 35.61817705367299 10-11 24.24216527761715 27.959275693512193 27.52094919928969 20.27760982958097 12-13 25.711556479475316 25.629757861451075 28.13651036481818 20.522175294255433 14-15 22.69972375164878 26.412789991230905 27.564044381266644 23.323441875853668 16-17 20.589176639718275 29.608924132829905 28.172197159082486 21.629702068369333 18-19 20.48101975133185 27.845262171026924 31.474468060576193 20.199250017065033 20-21 21.949595751109097 26.543966149999843 31.520287290201132 19.986150808689928 22-23 21.324356115892552 26.753177880807694 31.378654215239482 20.543811788060275 24-25 21.08933719372365 26.927576688839938 31.717775479664965 20.265310637771446 26-27 20.91376635630834 26.967226900137238 31.712164476187287 20.406842267367136 28-29 20.60258854593496 27.323723433108366 31.73854814936278 20.335139871593892 30-31 20.957972687143226 26.875550865413565 31.610444454003712 20.556031993439497 32-33 20.873777613325874 26.94849428605884 31.532072604539348 20.64565549607594 34-35 21.230883367795688 26.99039989078432 31.472598083198992 20.306118658220996 36-37 21.243309853419376 26.778925143750186 31.479154049575946 20.498610953254488 38-39 20.884969680221733 27.162440259468124 31.52936676637529 20.423223293934857 40-41 21.09938686055511 27.085562627684006 31.350105496293768 20.464945015467116 42-43 20.976105205174616 27.189611977227017 30.927041909680348 20.90724090791802 44-45 21.216965696277562 27.274106420174256 30.68229917820613 20.826628705342053 46-47 21.61294608251113 27.620498000575346 29.998012653141 20.768543263772532 48-49 21.486252003356892 27.82022692328667 29.768391996600652 20.925129076755788 50-51 21.405252021873604 27.953022480064917 29.6638512753075 20.97787422275398 52-53 21.626744087099816 28.19701950852177 29.23489372837762 20.941342676000794 54-55 21.4882126391369 28.071955000400763 29.23778982546149 21.20204253500085 56-57 21.610740342896044 28.296993575045338 28.969756415347426 21.122509666711196 58-59 21.75025816110241 28.006147189616975 29.190879277591762 21.052715371688855 60-61 21.477833521939566 28.153780444778874 29.54107931563021 20.827306717651346 62-63 21.305511591142118 27.940029795154196 29.680654578420164 21.073804035283523 64-65 21.3639083737009 28.07339180911981 29.408312394658488 21.154387422520806 66-67 21.408621018731218 28.13719377793995 28.9711582925879 21.483026910740925 68-69 21.396969411385797 28.144947082273863 29.11751331063579 21.340570195704547 70-71 21.58705549659923 28.017729719679597 29.074496701160236 21.320718082560937 72-73 21.501902428043564 27.877626018700347 29.10035925601246 21.520112297243628 74-75 21.299500712888225 28.5050978233439 28.699007812581495 21.49639365118638 76-77 21.39012830878141 28.279190275221367 28.815106449403917 21.515574966593306 78-79 21.45234610586159 28.30815411951874 28.701320639073575 21.53817913554609 80-81 21.538947177721518 28.271113677100708 28.52547603732722 21.66446310785056 82-83 21.56463694247001 28.28148627205328 28.736391324485943 21.417485460990765 84-85 21.40763676156805 28.334321508174405 28.538800502139217 21.71924122811833 86-87 21.248486636284717 28.451109751259366 28.543961111493367 21.75644250096255 88-89 21.44663188389672 28.361304055588505 28.36953890168747 21.822525158827307 90-91 21.32099627091958 28.247811649414466 28.613180280854035 21.81801179881192 92-93 21.41195682865285 28.367360868770387 28.483507522815305 21.73717477976146 94-95 21.313208466981028 28.31650018958331 28.350427550627526 22.01986379280813 96-97 21.265613336946867 28.283862849860714 28.422261745011884 22.02826206818053 98-99 21.16162906367414 28.402367513591027 28.092630506405186 22.343372916329653 100-101 21.140442439139363 28.473363910657216 28.10318236450649 22.283011285696926 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1144.0 1 817.5 2 592.0 3 723.0 4 623.0 5 642.5 6 1174.0 7 2560.0 8 4365.0 9 5258.5 10 4725.0 11 3947.5 12 3944.5 13 4314.5 14 4586.5 15 4866.0 16 5251.5 17 5683.0 18 6119.5 19 6636.0 20 7394.0 21 8502.5 22 10144.5 23 12430.0 24 14737.0 25 17539.0 26 22439.5 27 27912.0 28 31484.5 29 35721.0 30 41576.5 31 46564.5 32 53034.0 33 60838.5 34 68222.5 35 74625.5 36 82863.5 37 89669.0 38 90228.0 39 89591.5 40 89738.5 41 90962.5 42 90988.0 43 91024.5 44 93299.0 45 95528.5 46 96303.5 47 99244.5 48 107016.5 49 104958.5 50 93719.0 51 85631.5 52 81093.5 53 78215.0 54 74144.5 55 67339.5 56 59060.0 57 52467.5 58 47854.0 59 42592.0 60 35264.0 61 28117.0 62 21566.0 63 15739.5 64 10995.0 65 7559.0 66 5381.0 67 4018.5 68 2990.5 69 2219.5 70 1556.5 71 941.0 72 602.5 73 444.5 74 318.5 75 199.5 76 129.0 77 78.0 78 41.5 79 23.5 80 14.0 81 8.5 82 4.0 83 5.0 84 4.0 85 0.5 86 0.5 87 2.0 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02254491048645768 2 0.0073197761319667804 3 0.007722363819224952 4 0.0 5 0.003769684707962891 6 0.0 7 0.0 8 1.463955226393356E-4 9 0.0 10-11 0.0 12-13 1.463955226393356E-4 14-15 0.0 16-17 9.149720164958475E-5 18-19 0.004702956164788656 20-21 0.0012626613827642695 22-23 0.0 24-25 0.004776153926108325 26-27 0.005837521465243507 28-29 0.007722363819224952 30-31 0.030248974865352718 32-33 5.672826502274255E-4 34-35 0.004227170716210816 36-37 0.015170236033501152 38-39 0.007191680049657361 40-41 0.00869223415671055 42-43 0.005087244411716913 44-45 0.0025436222058584563 46-47 0.0013907574650736882 48-49 0.0012809608230941864 50-51 0.002964509333446546 52-53 0.0020678367572806154 54-55 0.0012077630617745188 56-57 3.293899259385051E-4 58-59 6.770792922069271E-4 60-61 0.0 62-63 1.829944032991695E-5 64-65 0.005087244411716913 66-67 4.940848889077576E-4 68-69 6.038815308872594E-4 70-71 0.0027632154898174595 72-73 0.010577076510691997 74-75 0.004702956164788656 76-77 0.0031841026174055493 78-79 0.01008299162178424 80-81 5.4898320989750854E-5 82-83 0.003861181909612476 84-85 5.123843292376746E-4 86-87 0.0 88-89 0.0011894636214446017 90-91 5.489832098975084E-4 92-93 9.149720164958475E-5 94-95 5.855820905573424E-4 96-97 1.6469496296925255E-4 98-99 2.1959328395900342E-4 100-101 5.855820905573424E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2732324.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.81865847222454 #Duplication Level Percentage of deduplicated Percentage of total 1 72.31164270076947 36.02469031275163 2 15.098925461168061 15.04416421695014 3 5.510404216133548 8.235628370623903 4 2.481046163423651 4.944095658777035 5 1.3011323559647867 3.2410334234485303 6 0.7665609314055821 2.291342234390702 7 0.4992810368611722 1.7411458019931423 8 0.34175296734857874 1.3620539489766519 9 0.2451899779427354 1.0993532194747262 >10 1.2526207771522617 11.506942387074085 >50 0.11681082165848676 4.032990886141827 >100 0.06897700387284343 6.325192877063712 >500 0.0038193569810243584 1.219847251905346 >1k 0.0015424326269337593 1.1706684203687272 >5k 1.4689834542226278E-4 0.5818031408077631 >10k+ 1.4689834542226278E-4 1.179047849252059 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17799 0.6514234768643836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14423 0.5278656557567843 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9971 0.3649274390592038 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5929 0.2169947634321552 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3808 0.1393685375526475 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.65988806598339E-5 0.0 2 0.0 0.0 0.0 3.65988806598339E-5 0.0 3 0.0 0.0 0.0 3.65988806598339E-5 0.0 4 0.0 0.0 0.0 1.097966419795017E-4 0.0 5 0.0 0.0 0.0 1.097966419795017E-4 0.0 6 0.0 0.0 0.0 1.097966419795017E-4 0.0 7 0.0 0.0 0.0 1.097966419795017E-4 0.0 8 0.0 0.0 0.0 1.097966419795017E-4 0.0 9 0.0 0.0 0.0 1.463955226393356E-4 0.0 10-11 0.0 0.0 0.0 1.463955226393356E-4 0.0 12-13 0.0 0.0 0.0 1.463955226393356E-4 0.0 14-15 0.0 0.0 0.0 1.463955226393356E-4 0.0 16-17 0.0 0.0 0.0 2.7449160494875426E-4 0.0 18-19 0.0 0.0 0.0 3.293899259385051E-4 0.0 20-21 0.0 0.0 0.0 3.8428824692825594E-4 0.0 22-23 0.0 0.0 0.0 5.672826502274254E-4 0.0 24-25 0.0 0.0 0.0 9.515708971556813E-4 0.0 26-27 0.0 0.0 0.0 0.0012626613827642695 0.0 28-29 0.0 0.0 0.0 0.00258022108651829 0.0 30-31 0.0 0.0 0.0 0.0059107192265631745 0.0 32-33 0.0 0.0 0.0 0.012352122222693942 0.0 34-35 0.0 0.0 0.0 0.02080646365511557 0.0 36-37 0.0 0.0 0.0 0.03288409427286076 0.0 38-39 0.0 0.0 0.0 0.05076264747518962 0.0 40-41 0.0 0.0 0.0 0.07235598706449162 0.0 42-43 0.0 0.0 0.0 0.09808500016835485 0.0 44-45 0.0 0.0 0.0 0.1280411839884289 0.0 46-47 0.0 0.0 0.0 0.16268202453296168 0.0 48-49 0.0 0.0 0.0 0.19959199567840416 0.0 50-51 0.0 0.0 0.0 0.24153431291457383 0.0 52-53 0.0 0.0 0.0 0.29021082419215294 0.0 54-55 0.0 0.0 0.0 0.3473965752231434 0.0 56-57 0.0 0.0 0.0 0.41237788783467844 0.0 58-59 0.0 0.0 0.0 0.4872591976646986 0.0 60-61 0.0 0.0 0.0 0.5694053853056958 0.0 62-63 0.0 0.0 0.0 0.6556140486999346 0.0 64-65 0.0 0.0 0.0 0.7454643007198267 0.0 66-67 0.0 0.0 0.0 0.8560661180738448 0.0 68-69 0.0 0.0 0.0 0.9820394653049931 0.0 70-71 0.0 0.0 0.0 1.1110871185115674 0.0 72-73 0.0 0.0 0.0 1.252431995619846 0.0 74-75 0.0 0.0 0.0 1.407574650736882 0.0 76-77 0.0 0.0 0.0 1.5758563040107982 0.0 78-79 0.0 0.0 0.0 1.7689702978124116 0.0 80-81 0.0 0.0 0.0 1.976028465145422 0.0 82-83 0.0 0.0 0.0 2.189912323721491 0.0 84-85 0.0 0.0 0.0 2.4154529257877178 0.0 86-87 0.0 1.829944032991695E-5 0.0 2.6715901920855654 0.0 88-89 0.0 3.65988806598339E-5 0.0 2.9502906683102004 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 17155 0.0 29.140133 1 GTATCAA 24245 0.0 27.71365 1 TATCAAC 29285 0.0 22.741873 2 ATCAACG 29435 0.0 22.60777 3 TCAACGC 29540 0.0 22.525349 4 GAGTACT 16050 0.0 22.430454 12-13 CAACGCA 29825 0.0 22.310102 5 AACGCAG 30345 0.0 21.926188 6 GTACATG 15565 0.0 21.492653 1 TACATGG 15330 0.0 21.412102 2 AGTACTT 16540 0.0 21.349585 12-13 GTACTTT 16850 0.0 21.184038 14-15 CGATTCG 160 4.9997332E-5 20.777351 9 ACATGGG 15580 0.0 20.304413 3 AGAGTAC 25960 0.0 19.986137 10-11 ACGCAGA 33705 0.0 19.627678 7 CGCAGAG 33855 0.0 19.428492 8 GCAGAGT 34765 0.0 18.619402 9 GTATAGG 860 0.0 18.234003 1 ACTTTTT 19715 0.0 18.129814 16-17 >>END_MODULE