FastQCFastQC Report
Thu 26 May 2016
SRR936761_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936761_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748349
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186150.6773157266417038No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136530.496770970499016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88870.32335776860944515No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44860.16322526724226072No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33200.12079979653239091No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30750.11188535371599458No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA214200.032.585021
GGTATCA136700.030.3779581
ATCAACG256300.027.1238233
TATCAAC257950.026.9503232
TCAACGC257500.026.942064
CAACGCA260450.026.5999285
AACGCAG264350.026.3143866
GAGTACT141000.023.85342412-13
GTACATG158300.023.6816391
ACGCAGA292600.023.5780547
TACATGG156100.023.317452
CGCAGAG295300.023.2011538
AGTACTT147900.022.05001812-13
GTACTTT153000.021.88743214-15
ACATGGG156400.021.8447633
GCAGAGT309450.021.6784529
AGAGTAC236150.021.22426810-11
ACTTTTT177200.018.99211116-17
CATGGGG59600.018.7359964
GTGTAGC18850.018.6524561
Produced by FastQC (version 0.11.5)