##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936761_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2748349 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.099362562760405 33.0 31.0 34.0 31.0 34.0 2 32.23226780878265 33.0 31.0 34.0 31.0 34.0 3 32.241252475577156 34.0 31.0 34.0 30.0 34.0 4 35.82432034650621 37.0 35.0 37.0 35.0 37.0 5 35.82857344536666 37.0 35.0 37.0 35.0 37.0 6 35.94003490823036 37.0 35.0 37.0 35.0 37.0 7 35.90560660236382 37.0 35.0 37.0 35.0 37.0 8 35.87672890160602 37.0 35.0 37.0 35.0 37.0 9 37.536102947624194 39.0 37.0 39.0 35.0 39.0 10-11 37.432829491451045 39.0 37.0 39.0 35.0 39.0 12-13 37.34681785319113 39.0 37.0 39.0 35.0 39.0 14-15 38.56485802931141 40.0 38.0 41.0 34.5 41.0 16-17 38.49770698699474 40.0 38.0 41.0 34.0 41.0 18-19 38.48915785440641 40.0 38.0 41.0 34.0 41.0 20-21 38.39874521030626 40.0 38.0 41.0 34.0 41.0 22-23 38.27905517094081 40.0 38.0 41.0 34.0 41.0 24-25 38.402176179226146 40.0 38.0 41.0 34.0 41.0 26-27 38.35684514594034 40.0 38.0 41.0 34.0 41.0 28-29 38.275514317868655 40.0 38.0 41.0 34.0 41.0 30-31 38.0862581498929 40.0 38.0 41.0 34.0 41.0 32-33 37.863973789354986 40.0 37.0 41.0 33.5 41.0 34-35 37.67849716320599 40.0 37.0 41.0 33.0 41.0 36-37 37.49770753277696 40.0 37.0 41.0 33.0 41.0 38-39 37.3428409201306 40.0 37.0 41.0 33.0 41.0 40-41 37.16974936589203 40.0 36.0 41.0 32.0 41.0 42-43 36.93116758461171 39.0 36.0 41.0 31.0 41.0 44-45 36.628691625408564 39.0 36.0 41.0 31.0 41.0 46-47 36.22347434769019 39.0 36.0 41.0 30.0 41.0 48-49 35.908580205789 39.0 35.5 40.5 29.0 41.0 50-51 35.19228507733188 38.5 35.0 40.0 28.0 40.5 52-53 35.38845921678797 38.5 35.0 40.0 28.0 40.5 54-55 35.80964790134004 39.0 36.0 40.5 28.5 41.0 56-57 35.723127594057374 39.0 35.0 41.0 28.0 41.0 58-59 35.496886312473414 39.0 35.0 41.0 28.0 41.0 60-61 35.23563692238504 39.0 35.0 40.5 27.5 41.0 62-63 34.925890052537 38.0 35.0 40.0 26.5 41.0 64-65 34.599005985047754 38.0 34.0 40.0 26.0 41.0 66-67 34.23450442429255 37.0 34.0 40.0 26.0 41.0 68-69 33.83549851201576 37.0 34.0 39.0 25.0 41.0 70-71 33.44248219567457 36.0 33.5 39.0 24.5 41.0 72-73 33.03771864490281 36.0 33.0 39.0 23.5 40.0 74-75 32.59615554647536 35.0 33.0 38.0 22.0 40.0 76-77 32.16519736030614 35.0 32.5 37.0 20.5 39.0 78-79 31.741641436367797 35.0 32.0 37.0 20.0 39.0 80-81 31.305744830805693 35.0 31.5 36.0 18.0 38.5 82-83 30.88943307418381 35.0 31.0 36.0 16.0 37.0 84-85 30.49532592112574 34.5 31.0 35.0 11.0 37.0 86-87 30.163175964915663 34.0 31.0 35.0 7.0 36.5 88-89 29.810256994290025 34.0 30.5 35.0 4.0 36.0 90-91 29.437541229298027 34.0 30.0 35.0 2.0 36.0 92-93 29.094773080129197 34.0 30.0 35.0 2.0 35.5 94-95 28.704937036744607 34.0 29.0 35.0 2.0 35.0 96-97 28.226431395721576 34.0 29.0 35.0 2.0 35.0 98-99 27.625280304648356 34.0 29.0 35.0 2.0 35.0 100-101 25.06106211401827 31.5 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3410401725316774 1101 2 0.4086443116126972 1101 3 0.23607779970883058 1101 4 0.34577163748582507 1101 5 0.34230018035236043 1101 6 0.177579695443427 1101 7 0.21992630186989715 1101 8 0.1627940278886797 1101 9 0.3115049506904839 1101 10-11 0.36632208941556144 1101 12-13 0.4142291453384175 1101 14-15 0.49616386104207066 1101 16-17 0.43433857677192833 1101 18-19 0.5419957047041208 1101 20-21 0.5457734832838597 1101 22-23 0.5187547039258291 1101 24-25 0.523874964956498 1101 26-27 0.5424239948562004 1101 28-29 0.4614692803613778 1101 30-31 0.49892236981993676 1101 32-33 0.4985007174756575 1101 34-35 0.5446784258823882 1101 36-37 0.45922745192930137 1101 38-39 0.4503614035308985 1101 40-41 0.4258236901383441 1101 42-43 0.3755610953854003 1101 44-45 0.37418583384249615 1101 46-47 0.2958264348716213 1101 48-49 0.3404657191986118 1101 50-51 0.4802499237764053 1101 52-53 0.5420453567618395 1101 54-55 0.5576001260722094 1101 56-57 0.5392018747054337 1101 58-59 0.4349134264666148 1101 60-61 0.6108351485889969 1101 62-63 0.6091190768915595 1101 64-65 0.614560950683881 1101 66-67 0.564698276165025 1101 68-69 0.653450749867666 1101 70-71 0.5169045883410206 1101 72-73 0.5232789157798905 1101 74-75 0.5226549609224449 1101 76-77 0.5926359078509122 1101 78-79 0.5518394331843233 1101 80-81 0.4844647138849538 1101 82-83 0.4328917034248825 1101 84-85 0.40853443424581926 1101 86-87 0.6335679688245257 1101 88-89 0.6956186052286029 1101 90-91 0.5338705076203958 1101 92-93 0.4537644698205092 1101 94-95 0.6880299143908779 1101 96-97 0.6686905756181645 1101 98-99 0.4889949966347231 1101 100-101 0.9041700456841149 1102 1 0.13216403574856628 1102 2 0.1335788731751606 1102 3 0.07384836247938864 1102 4 0.06697117438303479 1102 5 0.10860106293231553 1102 6 0.05052257943282257 1102 7 0.07019896202627507 1102 8 0.13241884751349886 1102 9 0.1417089843828876 1102 10-11 0.19632896884105122 1102 12-13 0.059900979963742884 1102 14-15 0.04238940552342996 1102 16-17 0.2407897160719017 1102 18-19 0.23352226599811843 1102 20-21 0.264436861249564 1102 22-23 0.2597342669984144 1102 24-25 -0.012113658822819673 1102 26-27 0.1570960476096488 1102 28-29 0.06783904109160943 1102 30-31 0.003051703019032459 1102 32-33 0.16250118729008278 1102 34-35 0.08626203607065008 1102 36-37 0.008522730294345138 1102 38-39 -0.05583930790237446 1102 40-41 0.05177254748254967 1102 42-43 0.07819876779051782 1102 44-45 -0.19664582158217314 1102 46-47 -0.15129637126280926 1102 48-49 -0.18940357171718603 1102 50-51 -0.0563700131342344 1102 52-53 -0.10155426067499462 1102 54-55 0.050878004524498976 1102 56-57 0.03113113407655277 1102 58-59 -0.1013331757683602 1102 60-61 -0.08297798272413814 1102 62-63 0.09970475090746334 1102 64-65 0.07832903014963932 1102 66-67 0.0018484322105294382 1102 68-69 0.10294444093111821 1102 70-71 -0.038050695393330614 1102 72-73 -0.10305166578324787 1102 74-75 0.03596832714278975 1102 76-77 0.049716262896389196 1102 78-79 -0.05570863889863631 1102 80-81 -0.10847464649626559 1102 82-83 0.015967058302560844 1102 84-85 0.029567019231890868 1102 86-87 0.07362258475123085 1102 88-89 0.1024829295347871 1102 90-91 -0.004071621310103524 1102 92-93 0.048671597576468884 1102 94-95 -0.011048494280554877 1102 96-97 -0.09020317920582244 1102 98-99 -0.14550867798523015 1102 100-101 0.10397876409748363 1103 1 -1.7561948111222137E-4 1103 2 0.10346627477611747 1103 3 0.12269331548035467 1103 4 0.08663188451689052 1103 5 0.1452370612051439 1103 6 0.0633443484566385 1103 7 0.035565820232008605 1103 8 0.041346173384560814 1103 9 0.028878081270654832 1103 10-11 0.0858417897797068 1103 12-13 0.17467080093560838 1103 14-15 0.1385074179457959 1103 16-17 0.2136739290456262 1103 18-19 0.17121449704073655 1103 20-21 0.226410820793852 1103 22-23 0.2056363827854497 1103 24-25 0.07828350794902406 1103 26-27 0.21349821321897622 1103 28-29 0.20675773109724815 1103 30-31 0.1947546292488198 1103 32-33 0.28406884597411164 1103 34-35 0.24843320888759735 1103 36-37 0.4069068270160372 1103 38-39 0.44291139791354794 1103 40-41 0.3525036897418232 1103 42-43 0.1889408709558964 1103 44-45 0.33480699669928526 1103 46-47 0.30886239580133434 1103 48-49 0.35756764760057536 1103 50-51 0.22260248495590673 1103 52-53 0.21150624020520326 1103 54-55 0.2281270662045145 1103 56-57 0.302218422746968 1103 58-59 0.16422259199328693 1103 60-61 0.2548213662644656 1103 62-63 0.24834294136151414 1103 64-65 0.20253685557230483 1103 66-67 0.056595667072848244 1103 68-69 0.1824209746276111 1103 70-71 0.15006461001736682 1103 72-73 -0.041076460681445326 1103 74-75 -0.03649156590534375 1103 76-77 -0.06692770694451156 1103 78-79 -0.0977781742226469 1103 80-81 0.050323556579431994 1103 82-83 0.006023357502083826 1103 84-85 -0.09644022160910737 1103 86-87 -0.09652679911298279 1103 88-89 0.10409551132639905 1103 90-91 -9.045837440879723E-4 1103 92-93 0.060660918996738644 1103 94-95 0.07783905678730108 1103 96-97 0.03259259918458923 1103 98-99 -0.11377270296280884 1103 100-101 0.08421425750433187 1104 1 0.28851036595594337 1104 2 0.23099552258497624 1104 3 0.25740603487533065 1104 4 0.1847883799565011 1104 5 0.3225058938644807 1104 6 0.27988757662242136 1104 7 0.23477976846357507 1104 8 0.27038885017598346 1104 9 0.36380835426130176 1104 10-11 0.338940201571873 1104 12-13 0.311812792524762 1104 14-15 0.44280837721233723 1104 16-17 0.39176004414929366 1104 18-19 0.34946685671492617 1104 20-21 0.4650118841765192 1104 22-23 0.4309128606143702 1104 24-25 0.4091167053460367 1104 26-27 0.44957637479954116 1104 28-29 0.3937775672579207 1104 30-31 0.35721479505228615 1104 32-33 0.5191196826898974 1104 34-35 0.5231762892434446 1104 36-37 0.42223342165309674 1104 38-39 0.4357531543051252 1104 40-41 0.4939415117756596 1104 42-43 0.43850554529547026 1104 44-45 0.44449142954938026 1104 46-47 0.5233183079076795 1104 48-49 0.5171062055215145 1104 50-51 0.34730458675334575 1104 52-53 0.41032153437278396 1104 54-55 0.3859092887300477 1104 56-57 0.5518985215865442 1104 58-59 0.6717908812679951 1104 60-61 0.5745672055727553 1104 62-63 0.7068368841500785 1104 64-65 0.6667764312678841 1104 66-67 0.4113102403340676 1104 68-69 0.5367820441024449 1104 70-71 0.43944501504657296 1104 72-73 0.45034004501165015 1104 74-75 0.431683574310199 1104 76-77 0.47540666019840216 1104 78-79 0.5485505984471608 1104 80-81 0.6352574187050486 1104 82-83 0.4905828468420417 1104 84-85 0.5540955374107526 1104 86-87 0.670196853307278 1104 88-89 0.7223894467078225 1104 90-91 0.5986418952416805 1104 92-93 0.5757080292541339 1104 94-95 0.6766970460561019 1104 96-97 0.6724525022425709 1104 98-99 0.6281253189972844 1104 100-101 0.677100602395857 1105 1 0.1847966702259427 1105 2 0.26168361484418057 1105 3 0.2762419622018726 1105 4 0.2003574458744808 1105 5 0.29820332210886846 1105 6 0.16940090478026804 1105 7 0.2331529112490074 1105 8 0.20680256062843227 1105 9 0.33295467525295663 1105 10-11 0.2948392023471058 1105 12-13 0.31511800834437764 1105 14-15 0.31949112267106017 1105 16-17 0.3524379176199375 1105 18-19 0.32609592772871565 1105 20-21 0.29032280752365125 1105 22-23 0.3455060077785035 1105 24-25 0.2510381309566796 1105 26-27 0.2295417363115959 1105 28-29 0.12330160857420225 1105 30-31 0.04133791597135428 1105 32-33 0.25587271559482616 1105 34-35 0.05099790537664006 1105 36-37 0.34557501440422556 1105 38-39 0.36910269500290127 1105 40-41 0.32927048777264645 1105 42-43 0.419645551390893 1105 44-45 0.38948609741702 1105 46-47 0.4265346458002668 1105 48-49 0.4339394458662227 1105 50-51 0.3901992147997717 1105 52-53 0.4834692091773647 1105 54-55 0.5176248011335716 1105 56-57 0.6376441536112765 1105 58-59 0.6135425369996668 1105 60-61 0.5004034105069977 1105 62-63 0.5633321710666053 1105 64-65 0.6601989757299407 1105 66-67 0.4921642724637607 1105 68-69 0.6493814138079941 1105 70-71 0.6622720828301141 1105 72-73 0.48901519890464584 1105 74-75 0.3514847180974101 1105 76-77 0.4107923529165518 1105 78-79 0.45173396023845314 1105 80-81 0.25529993451537436 1105 82-83 0.3896859729438482 1105 84-85 0.5096992200861337 1105 86-87 0.5028227668729492 1105 88-89 0.4470658924816924 1105 90-91 0.5832541163912595 1105 92-93 0.6720701731244638 1105 94-95 0.8032884417383492 1105 96-97 0.672495474394335 1105 98-99 0.5451218100576121 1105 100-101 0.7276987627966136 1106 1 0.1238723274568585 1106 2 0.22652967237596044 1106 3 0.2736235872546189 1106 4 0.27447616938803066 1106 5 0.3400258881071423 1106 6 0.265195406605649 1106 7 0.2409282327555502 1106 8 0.2675711951658428 1106 9 0.3042339594078669 1106 10-11 0.33519010770218927 1106 12-13 0.3607251557879181 1106 14-15 0.4420116582706868 1106 16-17 0.31766596482314924 1106 18-19 0.2687713918722494 1106 20-21 0.4450031699408754 1106 22-23 0.4490418344309859 1106 24-25 0.45134163588247134 1106 26-27 0.3042488947624875 1106 28-29 0.41901286726542963 1106 30-31 0.3968485311908623 1106 32-33 0.38874681666071353 1106 34-35 0.49673593716955367 1106 36-37 0.3545017512733679 1106 38-39 0.32566044234737745 1106 40-41 0.41109447555447787 1106 42-43 0.3801717947635481 1106 44-45 0.59993884375249 1106 46-47 0.5111973849809459 1106 48-49 0.4450432314796089 1106 50-51 0.3264627790735588 1106 52-53 0.3693810540103257 1106 54-55 0.34577061191711067 1106 56-57 0.4755460941165168 1106 58-59 0.29923188417169655 1106 60-61 0.29606484944368816 1106 62-63 0.3465141914669019 1106 64-65 0.32426746671307427 1106 66-67 0.32126027037237037 1106 68-69 0.26207593443761823 1106 70-71 0.29050824566560607 1106 72-73 0.21725927327768346 1106 74-75 0.3525174030668623 1106 76-77 0.40669366049878164 1106 78-79 0.429808968493969 1106 80-81 0.3673932356216163 1106 82-83 0.45427874661424994 1106 84-85 0.5367879233277968 1106 86-87 0.6505612710649196 1106 88-89 0.5248199674968284 1106 90-91 0.5202509311374506 1106 92-93 0.4817041899840646 1106 94-95 0.5265919902349303 1106 96-97 0.6039526649500218 1106 98-99 0.6477792840527314 1106 100-101 0.7208681247368425 1107 1 0.16056790768035967 1107 2 0.2107928182825063 1107 3 0.2234297455633154 1107 4 0.16716619932424948 1107 5 0.2839065104458882 1107 6 0.17272850379610816 1107 7 0.07583600687652137 1107 8 0.18205516720058057 1107 9 0.2556040994201396 1107 10-11 0.24718900136352318 1107 12-13 0.2972529258182277 1107 14-15 0.263778697280749 1107 16-17 0.33692108047738145 1107 18-19 0.33713434765589056 1107 20-21 0.45546324180639886 1107 22-23 0.42270150280829455 1107 24-25 0.30212920491242556 1107 26-27 0.25583937281236047 1107 28-29 0.2824455851397758 1107 30-31 0.23744366469525602 1107 32-33 0.17333413606719006 1107 34-35 0.14827255093648972 1107 36-37 0.3152966881469794 1107 38-39 0.2112360870917982 1107 40-41 0.24397237388288318 1107 42-43 0.37787683929600036 1107 44-45 0.3436174895567703 1107 46-47 0.35111424688385284 1107 48-49 0.2991663186293536 1107 50-51 0.27689107856594575 1107 52-53 0.391232288647096 1107 54-55 0.44889067290519336 1107 56-57 0.41388543561258473 1107 58-59 0.3562824108446918 1107 60-61 0.4396493053853803 1107 62-63 0.43234341089384287 1107 64-65 0.5804493083486051 1107 66-67 0.49957707835287835 1107 68-69 0.551644915583374 1107 70-71 0.45175059891303704 1107 72-73 0.5529353252291216 1107 74-75 0.5383429618143722 1107 76-77 0.5529226377398189 1107 78-79 0.506006591725221 1107 80-81 0.4524912488604933 1107 82-83 0.4271485798267207 1107 84-85 0.5107136010764464 1107 86-87 0.5300446736606297 1107 88-89 0.5356997972694231 1107 90-91 0.5408077379093577 1107 92-93 0.46657680996137785 1107 94-95 0.30834989497823884 1107 96-97 0.2808441321968189 1107 98-99 0.13313087197411377 1107 100-101 0.115843696964955 1108 1 -0.060618182033650214 1108 2 0.028409043005332535 1108 3 -0.08903875040772391 1108 4 0.06704609945796136 1108 5 0.13969496902622325 1108 6 0.1445535484637972 1108 7 -0.0754803586530457 1108 8 0.029771494866146497 1108 9 0.11296273063663875 1108 10-11 0.039123674135751685 1108 12-13 0.09958629464905755 1108 14-15 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-1.6191284720637924 2316 98-99 -1.6809617847421308 2316 100-101 -1.9093757393028241 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4105.0 3 1257.0 4 1211.0 5 1300.0 6 1407.0 7 1745.0 8 2023.0 9 2633.0 10 3388.0 11 4555.0 12 6975.0 13 9616.0 14 11269.0 15 15099.0 16 21067.0 17 30769.0 18 26968.0 19 13655.0 20 12234.0 21 12323.0 22 12950.0 23 14121.0 24 15788.0 25 17777.0 26 21642.0 27 25779.0 28 31457.0 29 39315.0 30 49316.0 31 64038.0 32 85773.0 33 117955.0 34 169613.0 35 253526.0 36 394946.0 37 597321.0 38 551175.0 39 102224.0 40 34.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.68618556595976 19.609653555520538 14.780037070829449 21.92412380769025 2 14.914825708651632 21.03278990972213 41.128267792207694 22.924116589418542 3 19.02286346223757 26.425059015454462 30.211109206702012 24.340968315605956 4 11.992124666098944 17.404633491276723 39.13833511656513 31.464906726059205 5 12.809769782072205 38.400622661243375 34.393369444093544 14.39623811259087 6 29.311518397025143 37.58384620200547 18.521948158404715 14.58268724256468 7 26.659686938446658 34.23818704652691 21.6500033843312 17.45212263069523 8 25.71042406672777 34.30485715804165 20.66649145554576 19.318227319684826 9 26.574510272058138 16.919161406410137 21.304803235348924 35.2015250861828 10-11 23.956683148949395 27.516295769814313 27.9123764147232 20.61464466651309 12-13 25.501409668733306 25.333181192478566 28.253266478710838 20.912142660077293 14-15 22.74802179208433 25.98359061363157 27.653042155975964 23.615345438308136 16-17 20.664161992540564 29.290076271985193 28.1284378162609 21.91732391921334 18-19 20.482679640211927 27.743253063828284 31.23032397593698 20.54374332002281 20-21 22.06393229443855 26.502078863063595 31.089387563720962 20.344601278776892 22-23 21.37923054208541 26.664793895396382 31.01156393806682 20.94441162445138 24-25 21.098135232104013 26.784986889189316 31.37530793985892 20.74156993884775 26-27 20.870406354943704 26.88354479062608 31.374332621936414 20.871716232493807 28-29 20.63319690920761 27.169365555042912 31.39095117986007 20.806486355889405 30-31 20.952852057726293 26.805784127125047 31.269918776691025 20.971445038457635 32-33 20.767295755384023 26.836158037914153 31.206340699050937 21.190205507650887 34-35 21.22056209392659 26.967777194194987 31.033000363672947 20.778660348205477 36-37 21.16843603195955 26.700011534197436 31.143624772545266 20.987927661297746 38-39 20.793415385107988 27.130517773235248 31.172457907410493 20.90360893424627 40-41 21.067354304241785 26.98936851860409 31.10959060118682 20.8336865759673 42-43 20.88720585733791 27.067083038080153 30.72962935000032 21.316081754581617 44-45 21.0949306810729 27.17856821235306 30.528435723648485 21.198065382925556 46-47 21.39108268110831 27.453141404738158 29.980295439242877 21.17548047491066 48-49 21.251166841347963 27.576970595143212 29.83961036822793 21.332252195280894 50-51 21.225672623223293 27.799740498278236 29.638938939230314 21.335647939268156 52-53 21.364007662821308 28.04405212610544 29.244669954718038 21.34727025635521 54-55 21.22786590280191 27.89794073074131 29.308264548512632 21.56592881794415 56-57 21.328538445067004 28.193983030141613 29.013185578876875 21.46429294591451 58-59 21.506165683610313 27.901608129922444 29.264151345014298 21.328074841452942 60-61 21.31275908461998 27.89103641125331 29.615056085537837 21.18114841858887 62-63 21.07950182212939 27.787223026053788 29.748493202423106 21.384781949393723 64-65 21.152842390682423 27.911683228047472 29.46298117953514 21.472493201734956 66-67 21.145402101951355 28.013833731401235 29.06686540040621 21.7738987662412 68-69 21.16511885180279 27.95692077031689 29.31423674289291 21.563723634987408 70-71 21.364606529236823 27.812210150722986 29.159976874838613 21.663206445201574 72-73 21.202693103262554 27.78619225971175 29.226815453792486 21.78429918323321 74-75 21.10594184723709 28.298869013263385 28.83839677771769 21.756792361781834 76-77 21.19533419993161 28.0926521310365 28.943982119176738 21.768031549855152 78-79 21.25427113952492 28.082617276420468 28.882747416754402 21.780364167300203 80-81 21.17402250788584 28.154877900734594 28.728813883230035 21.94228570814953 82-83 21.344063850239625 28.105457866245132 28.894814024311454 21.655664259203785 84-85 21.291914566241477 28.13482534350206 28.678707776099486 21.89455231415697 86-87 21.114848605984363 28.23406369445758 28.79884249458284 21.852245204975222 88-89 21.328776554825083 28.10737578029646 28.578919750188682 21.98492791468977 90-91 21.232660968283554 28.051996214341358 28.788711152826224 21.926631664548864 92-93 21.4074538058828 28.07371768741156 28.741679817844062 21.777148688861583 94-95 21.259831344912154 28.00698098950359 28.69434499457703 22.038842671007224 96-97 21.20645840528324 28.04379479165795 28.74435788418701 22.00538891887181 98-99 21.137911789053074 28.12771882089634 28.434237585525995 22.300131804524593 100-101 21.274430367582987 27.931607710557792 28.520750206202607 22.27321171565662 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 753.0 1 568.5 2 570.0 3 782.0 4 666.5 5 655.0 6 1255.5 7 2740.0 8 4765.5 9 5721.0 10 4953.5 11 4086.5 12 4130.0 13 4375.0 14 4511.0 15 4699.5 16 4886.0 17 5237.0 18 5608.5 19 6030.0 20 6795.0 21 7826.0 22 9317.0 23 11566.5 24 13812.5 25 16744.0 26 21561.5 27 26994.0 28 30511.5 29 34185.0 30 40111.0 31 45930.0 32 52719.5 33 60145.5 34 67481.0 35 74134.5 36 83022.0 37 89998.5 38 90006.5 39 89720.5 40 90023.5 41 90838.0 42 91051.0 43 91301.5 44 93633.5 45 96432.0 46 97337.0 47 99601.5 48 108106.5 49 106765.5 50 95269.0 51 87762.0 52 83405.5 53 80304.5 54 75878.5 55 68630.5 56 60788.0 57 54313.0 58 49959.5 59 44633.0 60 36728.5 61 29305.5 62 22340.5 63 16343.5 64 11259.5 65 7670.5 66 5525.5 67 4078.5 68 3010.0 69 2232.5 70 1566.0 71 942.5 72 636.0 73 500.0 74 323.5 75 199.5 76 139.5 77 85.5 78 55.5 79 33.0 80 24.0 81 20.5 82 16.5 83 10.5 84 9.5 85 9.0 86 10.0 87 9.0 88 7.5 89 6.0 90 3.5 91 4.5 92 3.0 93 2.0 94 2.5 95 0.5 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02314116584174717 2 0.010115163685543577 3 0.012371063500305092 4 0.019247919387239392 5 0.05203123766304789 6 0.03845945329359554 7 0.014226723025350857 8 0.043189565808418075 9 0.025506222099158444 10-11 0.020012014485787648 12-13 0.032565005390508994 14-15 0.017119368755569252 16-17 0.015281901970965114 18-19 0.014481421391533607 20-21 0.012098172393680716 22-23 0.007040590550908927 24-25 0.001619153899304637 26-27 1.8192740441625136E-5 28-29 0.0020739724103452655 30-31 0.0 32-33 3.0927658750762733E-4 34-35 1.8192740441625136E-5 36-37 0.0 38-39 1.0915644264975082E-4 40-41 7.277096176650054E-5 42-43 5.457822132487541E-5 44-45 5.457822132487541E-5 46-47 1.8192740441625136E-5 48-49 3.820475492741279E-4 50-51 1.8192740441625135E-4 52-53 5.093967323655038E-4 54-55 0.001600961158863012 56-57 2.001201448578765E-4 58-59 0.0011825281287056338 60-61 0.0033292715008173997 62-63 0.0019466232272538895 64-65 0.001364455533121885 66-67 0.010315283830401452 68-69 0.014153952063584356 70-71 0.02519694551165081 72-73 0.01604599706951337 74-75 0.01757418726660988 76-77 0.029999828988239847 78-79 0.01986647256225465 80-81 0.042880289220910445 82-83 0.03347464241259025 84-85 0.018647558952665764 86-87 0.013480820667244224 88-89 0.012916845713553848 90-91 0.02221333607922429 92-93 0.02958139595808247 94-95 0.011443233737782211 96-97 0.017756114671026134 98-99 0.02617935349549857 100-101 0.038241140408296036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2748349.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.465350048573285 #Duplication Level Percentage of deduplicated Percentage of total 1 71.88907048108659 35.56018036013505 2 15.33801613313168 15.17400674152046 3 5.478702465302434 8.130178057744992 4 2.546776467794185 5.039087578996336 5 1.371502655945073 3.3920929484435502 6 0.7961191700116603 2.3628188055003845 7 0.4997579814566699 1.7304492444625803 8 0.34526994886175305 1.3663119105359691 9 0.2570775920675916 1.144478977314097 >10 1.2857257704107894 11.68833915424183 >50 0.11646232342191203 3.996184703986417 >100 0.07006758468671302 6.4465250904375395 >500 0.003904399575914031 1.2898699642515128 >1k 0.0013260224974665396 1.179784443943591 >5k 7.366791652591886E-5 0.3238431045628457 >10k+ 1.4733583305183773E-4 1.175848913923023 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18615 0.6773157266417038 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13653 0.496770970499016 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8887 0.32335776860944515 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4486 0.16322526724226072 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3320 0.12079979653239091 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3075 0.11188535371599458 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.277096176650054E-5 0.0 10-11 0.0 0.0 0.0 7.277096176650054E-5 0.0 12-13 0.0 0.0 0.0 7.277096176650054E-5 0.0 14-15 0.0 0.0 0.0 9.096370220812568E-5 0.0 16-17 0.0 0.0 0.0 1.4554192353300109E-4 0.0 18-19 0.0 0.0 0.0 1.6373466397462622E-4 0.0 20-21 0.0 0.0 0.0 2.0012014485787648E-4 0.0 22-23 0.0 0.0 0.0 4.548185110406284E-4 0.0 24-25 0.0 0.0 0.0 6.913241367817551E-4 0.0 26-27 0.0 0.0 0.0 9.278297625228819E-4 0.0 28-29 0.0 0.0 0.0 0.0019284304868122642 0.0 30-31 0.0 0.0 0.0 0.004893847178797162 0.0 32-33 0.0 0.0 0.0 0.009933236281127324 0.0 34-35 0.0 0.0 0.0 0.017919849335000758 0.0 36-37 0.0 0.0 0.0 0.02843525331026009 0.0 38-39 0.0 0.0 0.0 0.04509980355478871 0.0 40-41 0.0 0.0 0.0 0.06376555524789611 0.0 42-43 0.0 0.0 0.0 0.08457805031311524 0.0 44-45 0.0 0.0 0.0 0.11128499328142095 0.0 46-47 0.0 0.0 0.0 0.14392276963369643 0.0 48-49 0.0 0.0 0.0 0.17887102402205834 0.0 50-51 0.0 0.0 0.0 0.21840384900170975 0.0 52-53 0.0 0.0 0.0 0.26170257125277757 0.0 54-55 0.0 0.0 0.0 0.3149527225254144 0.0 56-57 0.0 0.0 0.0 0.37589840300485855 0.0 58-59 0.0 0.0 0.0 0.44632250125438944 0.0 60-61 0.0 0.0 0.0 0.5249151399622101 0.0 62-63 0.0 0.0 0.0 0.6052542817524267 0.0 64-65 0.0 0.0 0.0 0.6921428101016283 0.0 66-67 0.0 0.0 0.0 0.8008444342403385 0.0 68-69 0.0 0.0 0.0 0.922535675054369 0.0 70-71 0.0 0.0 0.0 1.0496665452604454 0.0 72-73 0.0 0.0 0.0 1.191297029598497 0.0 74-75 0.0 1.8192740441625136E-5 0.0 1.3457715886883361 0.0 76-77 0.0 3.638548088325027E-5 0.0 1.5108889009365258 0.0 78-79 0.0 3.638548088325027E-5 0.0 1.702240144901539 0.0 80-81 0.0 3.638548088325027E-5 0.0 1.915786532205335 0.0 82-83 0.0 3.638548088325027E-5 0.0 2.1318253249496335 0.0 84-85 0.0 3.638548088325027E-5 0.0 2.362891321298714 0.0 86-87 0.0 3.638548088325027E-5 0.0 2.619772816334461 0.0 88-89 0.0 5.457822132487541E-5 0.0 2.904525589726778 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 21420 0.0 32.58502 1 GGTATCA 13670 0.0 30.377958 1 ATCAACG 25630 0.0 27.123823 3 TATCAAC 25795 0.0 26.950323 2 TCAACGC 25750 0.0 26.94206 4 CAACGCA 26045 0.0 26.599928 5 AACGCAG 26435 0.0 26.314386 6 GAGTACT 14100 0.0 23.853424 12-13 GTACATG 15830 0.0 23.681639 1 ACGCAGA 29260 0.0 23.578054 7 TACATGG 15610 0.0 23.31745 2 CGCAGAG 29530 0.0 23.201153 8 AGTACTT 14790 0.0 22.050018 12-13 GTACTTT 15300 0.0 21.887432 14-15 ACATGGG 15640 0.0 21.844763 3 GCAGAGT 30945 0.0 21.678452 9 AGAGTAC 23615 0.0 21.224268 10-11 ACTTTTT 17720 0.0 18.992111 16-17 CATGGGG 5960 0.0 18.735996 4 GTGTAGC 1885 0.0 18.652456 1 >>END_MODULE