FastQCFastQC Report
Thu 26 May 2016
SRR936761_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936761_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2748349
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176840.6434408439393978No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146350.5325015127263677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98180.3572326513117512No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT56880.20696061526392753No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38180.13891976601224953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA159600.030.1534771
GTATCAA227550.027.6630361
ATCAACG277400.022.5487043
TATCAAC280250.022.4400672
GAGTACT158600.022.29481112-13
TCAACGC280600.022.2915574
CAACGCA283400.022.0254485
AACGCAG289400.021.6016336
TACATGG155250.021.5694642
GTACATG158350.021.4510631
GTACTTT168750.020.8414314-15
AGTACTT165000.020.69604112-13
ACATGGG157100.020.4065633
AGAGTAC251600.020.1079710-11
ACGCAGA323550.019.2335347
CGCAGAG324600.019.0688788
CATGGGG62850.018.3629864
GCAGAGT335850.018.1189489
ACTTTTT197200.018.06393816-17
TACTTTT197600.017.37791314-15
Produced by FastQC (version 0.11.5)