##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936761_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2748349 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.690426143113555 31.0 31.0 34.0 30.0 34.0 2 31.8259882569499 31.0 31.0 34.0 30.0 34.0 3 31.915322617324072 31.0 31.0 34.0 30.0 34.0 4 35.68080436654879 37.0 35.0 37.0 33.0 37.0 5 35.567756132863764 37.0 35.0 37.0 33.0 37.0 6 35.61931763396861 37.0 35.0 37.0 33.0 37.0 7 35.55205070389532 37.0 35.0 37.0 33.0 37.0 8 35.512295199772666 37.0 35.0 37.0 33.0 37.0 9 37.08416216426662 39.0 37.0 39.0 33.0 39.0 10-11 36.96603197046663 39.0 37.0 39.0 33.0 39.0 12-13 36.846730164182205 39.0 37.0 39.0 33.0 39.0 14-15 37.84072255743357 39.5 37.0 41.0 33.0 41.0 16-17 37.72400903233178 39.0 37.0 41.0 32.5 41.0 18-19 37.958651539524276 39.5 37.0 41.0 33.0 41.0 20-21 38.00677261148421 40.0 37.0 41.0 33.0 41.0 22-23 37.904012918301135 40.0 37.0 41.0 33.0 41.0 24-25 37.7682574156339 39.5 37.0 41.0 32.0 41.0 26-27 37.519598311568146 39.0 36.5 41.0 32.0 41.0 28-29 37.40682842681188 39.0 36.0 41.0 32.0 41.0 30-31 37.34379531129416 39.0 36.0 41.0 32.0 41.0 32-33 37.10847003055289 39.0 36.0 40.5 31.0 41.0 34-35 36.90486088193312 39.0 35.0 40.0 31.0 41.0 36-37 36.689234154759816 39.0 35.0 40.0 30.5 41.0 38-39 36.48846925918069 39.0 35.0 40.0 30.0 41.0 40-41 36.25022604479999 38.0 35.0 40.0 30.0 41.0 42-43 35.913690910433864 38.0 35.0 40.0 29.5 41.0 44-45 35.55713102666364 38.0 35.0 40.0 28.5 41.0 46-47 35.10369898437207 38.0 34.0 40.0 26.5 41.0 48-49 34.63622305609658 38.0 34.0 40.0 24.5 41.0 50-51 34.28364247044317 38.0 33.0 40.0 24.0 41.0 52-53 33.98936943597775 38.0 33.0 40.0 23.0 41.0 54-55 33.66844039821726 38.0 33.0 40.0 22.0 41.0 56-57 33.32491597682827 37.0 32.5 40.0 20.5 41.0 58-59 32.915255486111846 37.0 31.5 39.0 19.5 40.0 60-61 32.52499500609275 36.0 31.0 39.0 18.0 40.0 62-63 32.099337638706004 36.0 31.0 39.0 16.0 40.0 64-65 31.666427189559986 35.0 30.5 38.5 13.0 40.0 66-67 31.189260716160867 35.0 30.0 38.0 9.5 40.0 68-69 30.80705707317375 34.5 29.0 38.0 8.0 40.0 70-71 30.260534597316422 34.0 29.0 37.0 7.0 39.0 72-73 29.682089137878776 34.0 28.0 36.5 6.5 39.0 74-75 29.078273174185668 33.5 27.5 36.0 2.0 39.0 76-77 27.872739051699767 31.5 26.0 34.5 2.0 36.5 78-79 28.608938311691855 33.0 27.0 35.0 2.0 37.0 80-81 28.353816418511624 33.0 27.0 35.0 2.0 37.0 82-83 27.917980394775192 33.0 26.0 35.0 2.0 36.5 84-85 27.4254494243635 32.5 26.0 35.0 2.0 36.0 86-87 26.937155907055473 32.0 25.5 35.0 2.0 35.5 88-89 26.378257637585328 32.0 25.0 34.0 2.0 35.0 90-91 25.707210765444998 31.5 23.0 34.0 2.0 35.0 92-93 24.996882310070518 31.0 19.5 34.0 2.0 35.0 94-95 24.133705362746873 31.0 14.5 34.0 2.0 35.0 96-97 23.13006153876382 30.0 2.0 34.0 2.0 35.0 98-99 21.900764786422684 30.0 2.0 34.0 2.0 35.0 100-101 18.22924090062798 24.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2633495663933445 1101 2 0.25857188153882404 1101 3 0.2543646375880577 1101 4 0.2820809148705621 1101 5 0.24716080448840927 1101 6 0.25328264701367686 1101 7 0.2431034588366714 1101 8 0.36730931813838197 1101 9 0.37550606095590666 1101 10-11 0.35554229105518687 1101 12-13 0.3749061062231718 1101 14-15 0.5139478773551218 1101 16-17 0.505290139907693 1101 18-19 0.45449231302215765 1101 20-21 0.4303012153361294 1101 22-23 0.5328477897482742 1101 24-25 0.5055312838945625 1101 26-27 0.41846953441676504 1101 28-29 0.5707989941023683 1101 30-31 0.6175951424435837 1101 32-33 0.7118984097461762 1101 34-35 0.7088645563771578 1101 36-37 0.640295046295293 1101 38-39 0.7396615996595344 1101 40-41 0.8527512755262663 1101 42-43 0.7578194655683461 1101 44-45 0.872474469001439 1101 46-47 0.7767505593574242 1101 48-49 0.7188994628750507 1101 50-51 0.705209147198893 1101 52-53 0.7104714535902943 1101 54-55 0.7784472810555272 1101 56-57 0.7972342861516921 1101 58-59 0.7649171754184323 1101 60-61 0.9440400584789543 1101 62-63 0.8368528973289884 1101 64-65 0.987715743381191 1101 66-67 0.6489465944500061 1101 68-69 0.7882200362834162 1101 70-71 0.6731476056863315 1101 72-73 0.8594451357370012 1101 74-75 0.7773477254014409 1101 76-77 0.5951852897216341 1101 78-79 0.7422484481703329 1101 80-81 1.0420661214182907 1101 82-83 1.0090227172170252 1101 84-85 0.8710301545562729 1101 86-87 0.5552361433924311 1101 88-89 0.47506429647586756 1101 90-91 0.714725319699042 1101 92-93 0.8152000682902987 1101 94-95 0.8413413683677291 1101 96-97 0.8000879297033165 1101 98-99 0.6502681901121363 1101 100-101 0.812133504007992 1102 1 0.29705959021965 1102 2 0.2377247054590903 1102 3 0.30931362916495786 1102 4 0.2132707198975794 1102 5 0.2568364647454615 1102 6 0.13823851801605969 1102 7 0.05231685525937024 1102 8 0.17920988527151138 1102 9 0.14979882245796716 1102 10-11 0.24001618279652348 1102 12-13 0.2864429866554019 1102 14-15 0.42323719914444524 1102 16-17 0.4704395576733944 1102 18-19 0.3540514425231507 1102 20-21 0.3548045543743541 1102 22-23 0.2664797908221388 1102 24-25 0.37436161377140564 1102 26-27 0.2770797226781099 1102 28-29 0.3942293105559429 1102 30-31 0.3372996627295066 1102 32-33 0.4509777412673728 1102 34-35 0.5238247899420472 1102 36-37 0.467214154319052 1102 38-39 0.5037354779775995 1102 40-41 0.5551477485622698 1102 42-43 0.6416272275737782 1102 44-45 0.5618612772835903 1102 46-47 0.6644657511423873 1102 48-49 0.8203336552976168 1102 50-51 0.8464076773509106 1102 52-53 0.7543521207267716 1102 54-55 0.8678722617595866 1102 56-57 0.8047865530062879 1102 58-59 0.9406051179668395 1102 60-61 0.9183386926613082 1102 62-63 0.9969421620694163 1102 64-65 0.865643274506386 1102 66-67 0.7457002606804188 1102 68-69 0.6984175261320686 1102 70-71 0.6683072586948242 1102 72-73 0.7971330615063259 1102 74-75 0.7093915859336732 1102 76-77 0.5887815513411496 1102 78-79 0.6396439144611499 1102 80-81 0.9484392379658253 1102 82-83 0.865327878464047 1102 84-85 0.9271834650700512 1102 86-87 0.818746663879697 1102 88-89 0.8034612225472095 1102 90-91 1.0479374241320762 1102 92-93 1.0403322035968507 1102 94-95 1.0218100921504067 1102 96-97 1.1420834664662927 1102 98-99 1.3684546225427567 1102 100-101 1.4269590854033432 1103 1 0.22861371384538387 1103 2 0.3306366528467386 1103 3 0.20465690481514187 1103 4 0.20723142670994577 1103 5 0.17024198505635013 1103 6 0.22952044580694064 1103 7 0.1427983047561625 1103 8 0.16356651329821403 1103 9 0.24102926720503604 1103 10-11 0.24737119990218304 1103 12-13 0.2773782342722342 1103 14-15 0.39399192395927685 1103 16-17 0.2956288824151514 1103 18-19 0.2557121858718716 1103 20-21 0.24425883410996363 1103 22-23 0.2954203196028189 1103 24-25 0.18599968797341404 1103 26-27 0.26932031456703953 1103 28-29 0.273058984718098 1103 30-31 0.3041744758958771 1103 32-33 0.2866334857786157 1103 34-35 0.48582905805245247 1103 36-37 0.28773056246334505 1103 38-39 0.18541125105362966 1103 40-41 0.38911244115868016 1103 42-43 0.5086967628977845 1103 44-45 0.2726909846606205 1103 46-47 0.33946616632822213 1103 48-49 0.448172280866423 1103 50-51 0.5695688192780395 1103 52-53 0.4495044108918549 1103 54-55 0.41452212723937265 1103 56-57 0.37731038467334344 1103 58-59 0.5340227617041791 1103 60-61 0.36965400139349214 1103 62-63 0.49713107162533987 1103 64-65 0.5270272549760513 1103 66-67 0.3680953847379449 1103 68-69 0.2718144936147162 1103 70-71 0.2630825601197415 1103 72-73 0.317994489081304 1103 74-75 0.5174723478827197 1103 76-77 0.3231095315450858 1103 78-79 0.48283154524468586 1103 80-81 0.5539720044320973 1103 82-83 0.46847291118069023 1103 84-85 0.4580172412682728 1103 86-87 0.30580366059142605 1103 88-89 0.350088252805957 1103 90-91 0.3615966229894845 1103 92-93 0.3894283440293087 1103 94-95 0.4170487409696193 1103 96-97 0.412718202574176 1103 98-99 0.32273352778074127 1103 100-101 0.5807314639199674 1104 1 0.21463939082906336 1104 2 0.2403931076719097 1104 3 0.22319775187596136 1104 4 0.14269118553671944 1104 5 0.21442454864334337 1104 6 0.028304488492054247 1104 7 0.11417157229632124 1104 8 0.23539498718554341 1104 9 0.1870277666345217 1104 10-11 0.34617909733074015 1104 12-13 0.2474608696835361 1104 14-15 0.26983698318728244 1104 16-17 0.3149363478991134 1104 18-19 0.4030212085202507 1104 20-21 0.3347271943767183 1104 22-23 0.31887713721150845 1104 24-25 0.3769098343435431 1104 26-27 0.22187715190037238 1104 28-29 0.2371837080946264 1104 30-31 0.23250460016109287 1104 32-33 0.34849904115200303 1104 34-35 0.35889675101059026 1104 36-37 0.3743791988417371 1104 38-39 0.5255689690878498 1104 40-41 0.4086466983596253 1104 42-43 0.3086948694990497 1104 44-45 0.3961821955452862 1104 46-47 0.30275821039155915 1104 48-49 0.2836793642583544 1104 50-51 0.5756635865897337 1104 52-53 0.597745828385861 1104 54-55 0.37078102725377704 1104 56-57 0.2631674659430132 1104 58-59 0.47645178838070734 1104 60-61 0.5601585755119416 1104 62-63 0.6174362750179725 1104 64-65 0.5569251133964279 1104 66-67 0.5135644295411694 1104 68-69 0.5744507184522725 1104 70-71 0.45448119296101197 1104 72-73 0.7717115012897757 1104 74-75 0.7489550122567792 1104 76-77 0.46551475549462396 1104 78-79 0.38394619532379437 1104 80-81 0.9601830181036881 1104 82-83 0.9048931537354719 1104 84-85 0.9426092408887641 1104 86-87 0.818661242467396 1104 88-89 0.7052676450540893 1104 90-91 0.7794268629392853 1104 92-93 0.6051825866989411 1104 94-95 0.6375076633718955 1104 96-97 0.8714140732171742 1104 98-99 0.6887653198181454 1104 100-101 0.8548593457352673 1105 1 0.2762359230687679 1105 2 0.2776006892415843 1105 3 0.28046088758266663 1105 4 0.2216786725459272 1105 5 0.18499269916213024 1105 6 0.2503626209581924 1105 7 0.30291362910322306 1105 8 0.2004276074491642 1105 9 0.37285564877021926 1105 10-11 0.28844375689187274 1105 12-13 0.3419009468921601 1105 14-15 0.3589441674682732 1105 16-17 0.31044833854193854 1105 18-19 0.2639320877543767 1105 20-21 0.3514510214298312 1105 22-23 0.4216884177544742 1105 24-25 0.4114523819347937 1105 26-27 0.43805336923189486 1105 28-29 0.4414939615977218 1105 30-31 0.43887398573537695 1105 32-33 0.28286665785996234 1105 34-35 0.4233226001451911 1105 36-37 0.4232144144929393 1105 38-39 0.6313060501433299 1105 40-41 0.6235566768626839 1105 42-43 0.7036545783532091 1105 44-45 0.6678615374574761 1105 46-47 0.6600861574472674 1105 48-49 0.6439547337183527 1105 50-51 0.7759945971089479 1105 52-53 0.7996492067792857 1105 54-55 0.8350692133889268 1105 56-57 0.7933811804156079 1105 58-59 0.7465116071371085 1105 60-61 0.7844995560049739 1105 62-63 0.7808756117582902 1105 64-65 0.49959379810632853 1105 66-67 0.6619397182178695 1105 68-69 0.6191306193827266 1105 70-71 0.26523368368886224 1105 72-73 0.38693803949994887 1105 74-75 0.7485122524124357 1105 76-77 0.48487487788105454 1105 78-79 0.6755598349346634 1105 80-81 0.6139786651496628 1105 82-83 0.688945704711255 1105 84-85 0.8479223046945279 1105 86-87 0.8007255572734486 1105 88-89 0.7273747255717318 1105 90-91 0.7730124951378663 1105 92-93 0.6746868097586365 1105 94-95 0.6936099533910038 1105 96-97 0.811856952579511 1105 98-99 0.6754546550644918 1105 100-101 0.8579482339843132 1106 1 0.1605023974124542 1106 2 0.23868624392062188 1106 3 0.1777139788153086 1106 4 0.2090187218955819 1106 5 0.166189611598611 1106 6 0.21624176476930757 1106 7 0.1937691529616643 1106 8 0.25536498017660136 1106 9 0.2228445267536756 1106 10-11 0.16196922974956607 1106 12-13 0.2529577219201329 1106 14-15 0.34010782851507315 1106 16-17 0.33439123100006185 1106 18-19 0.07764971924642339 1106 20-21 0.28191806586286106 1106 22-23 0.26885241819476136 1106 24-25 0.293982617767405 1106 26-27 0.3523544479528411 1106 28-29 0.3681553844820158 1106 30-31 0.2904777346575713 1106 32-33 0.42195676224639556 1106 34-35 0.34327096876322116 1106 36-37 0.4339848835897868 1106 38-39 0.4703438695859887 1106 40-41 0.6072612600507838 1106 42-43 0.4081357190822672 1106 44-45 0.21479272084003753 1106 46-47 0.37749647042311096 1106 48-49 0.5611209929599497 1106 50-51 0.378200884144114 1106 52-53 0.42467242542208083 1106 54-55 0.17265497904229932 1106 56-57 0.4241234660932065 1106 58-59 0.49615581726754243 1106 60-61 0.3508592970569069 1106 62-63 0.5674029512802079 1106 64-65 0.3529091336472483 1106 66-67 0.4131172187223733 1106 68-69 0.20638455909910292 1106 70-71 0.11744874221130885 1106 72-73 0.30644250206576373 1106 74-75 0.3458551223972073 1106 76-77 0.31142141148100677 1106 78-79 0.35024893444116856 1106 80-81 0.41706124096283403 1106 82-83 0.5351486826598482 1106 84-85 0.6300274960390766 1106 86-87 0.4348867737698079 1106 88-89 0.42881399477498405 1106 90-91 0.4843416449112965 1106 92-93 0.6046678679325161 1106 94-95 0.45173017207048716 1106 96-97 0.49462155337938185 1106 98-99 0.44505302414115633 1106 100-101 0.44807859089784685 1107 1 0.14343816933934406 1107 2 0.13334955438342888 1107 3 0.16156108560023696 1107 4 0.17032348101125194 1107 5 0.1272992506904842 1107 6 0.07745006788751141 1107 7 0.10708809924381057 1107 8 0.17384862216913177 1107 9 0.22969092696936144 1107 10-11 0.2598532408315535 1107 12-13 0.14638953334053184 1107 14-15 0.2582936190358893 1107 16-17 0.1664912640042857 1107 18-19 0.15605827663658545 1107 20-21 0.16127224066437407 1107 22-23 0.1929303918285754 1107 24-25 0.18885691119817238 1107 26-27 0.16769188124712286 1107 28-29 0.19605952349022004 1107 30-31 0.13027024967217216 1107 32-33 0.298856364336352 1107 34-35 0.3031101196715369 1107 36-37 0.2930431476607396 1107 38-39 0.38436345828831975 1107 40-41 0.33879696736377696 1107 42-43 0.322911150926231 1107 44-45 0.21755490683509038 1107 46-47 0.07235025242790272 1107 48-49 0.19583138092224317 1107 50-51 0.11087111154784424 1107 52-53 0.16663676924239468 1107 54-55 0.3957586308896097 1107 56-57 0.3334821383008588 1107 58-59 0.303046610484472 1107 60-61 0.43533470426391574 1107 62-63 0.3316195193990623 1107 64-65 0.37616473274358597 1107 66-67 0.39858031210107825 1107 68-69 0.4972210069152432 1107 70-71 0.4500603406382595 1107 72-73 0.4324699327568986 1107 74-75 0.4251905289534257 1107 76-77 0.4484210024146087 1107 78-79 0.7452815086774223 1107 80-81 0.9509328103832608 1107 82-83 0.891144236387003 1107 84-85 0.8796333628879651 1107 86-87 0.7891690714213944 1107 88-89 0.9641763323105472 1107 90-91 0.9575521537341167 1107 92-93 0.8153456630646261 1107 94-95 0.8529838676135242 1107 96-97 0.7300756984306069 1107 98-99 0.6716620961182471 1107 100-101 0.698638442425338 1108 1 0.08946718262724218 1108 2 0.06299943073381087 1108 3 -0.006795261943931763 1108 4 0.06152246814932738 1108 5 0.0565367644457595 1108 6 0.07515160492914674 1108 7 0.09120296914548476 1108 8 -0.019266638205010622 1108 9 0.024673947333091917 1108 10-11 0.09960659238693381 1108 12-13 0.04841851113092588 1108 14-15 0.043629307036553655 1108 16-17 0.12228770952154377 1108 18-19 0.11816233163403922 1108 20-21 0.1194399190097144 1108 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2316 50-51 -1.6615965308397094 2316 52-53 -1.826272564558117 2316 54-55 -1.6883380703029012 2316 56-57 -1.6196682635874282 2316 58-59 -1.7147635759723698 2316 60-61 -1.7350448060300323 2316 62-63 -1.8472617539549105 2316 64-65 -1.5704414758519043 2316 66-67 -2.022882663638555 2316 68-69 -2.1995890476094893 2316 70-71 -1.8893735191979282 2316 72-73 -1.6014482996123185 2316 74-75 -1.6890822187022536 2316 76-77 -1.5205718509021153 2316 78-79 -1.9585917442133685 2316 80-81 -2.0722881066963303 2316 82-83 -1.8115460252206823 2316 84-85 -1.771020998766076 2316 86-87 -1.9029900516842453 2316 88-89 -2.1118968056623295 2316 90-91 -2.4894936843844597 2316 92-93 -2.756638300334668 2316 94-95 -2.5306385989114055 2316 96-97 -1.984415061635346 2316 98-99 -2.393933325044383 2316 100-101 -1.997279304029977 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 12.0 4 32.0 5 93.0 6 186.0 7 537.0 8 1268.0 9 2387.0 10 4002.0 11 6560.0 12 9617.0 13 13557.0 14 17576.0 15 23747.0 16 30462.0 17 39510.0 18 35493.0 19 24293.0 20 22669.0 21 23419.0 22 25294.0 23 28845.0 24 32765.0 25 39401.0 26 47174.0 27 57480.0 28 70031.0 29 84884.0 30 101468.0 31 122725.0 32 150001.0 33 185512.0 34 236919.0 35 309649.0 36 403783.0 37 424099.0 38 167867.0 39 5026.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.71148023059454 21.229437197926977 15.791249344901882 21.267833226576602 2 15.755667959992884 22.672422168457953 39.30167661798894 22.270233253560225 3 19.668091160316482 26.704653163995058 30.889899193878495 22.73735648180996 4 13.057208267960634 18.71410565780703 38.008742719252034 30.219943354980305 5 12.70551884058393 38.96550256171978 34.2626427720788 14.066335825617488 6 29.323204585734924 38.50238816103777 17.75505949208052 14.419347761146783 7 26.42142609981483 34.63297419650852 21.44403785690973 17.501561846766915 8 25.961149766641718 34.9936270830233 20.18983033086409 18.85539281947089 9 26.43283399075881 16.887459989586468 21.082054049215763 35.59765197043896 10-11 24.211879417300672 27.94131159203405 27.528167470108595 20.31864152055668 12-13 25.704413813529502 25.58357763151623 28.126176842897316 20.585831712056947 14-15 22.732866168015782 26.393718556122238 27.534785429361406 23.338629846500574 16-17 20.591598810776944 29.601699056415328 28.16245316733792 21.644248965469814 18-19 20.497052947252868 27.808996633966913 31.43762468894849 20.25632572983173 20-21 21.964971097908347 26.535745973782994 31.47234293692116 20.0269399913875 22-23 21.34575653731873 26.72363040689183 31.342507253580216 20.58810580220922 24-25 21.165695741770488 26.899653465508667 31.634709051853537 20.299941740867308 26-27 20.923093437352744 26.967202228363607 31.698417694458747 20.411286639824905 28-29 20.639421760570233 27.289357130706964 31.672271560040276 20.398949548682523 30-31 21.019932652410354 26.889441194027025 31.56371104176267 20.526915111799944 32-33 20.88023928128286 26.93952107356371 31.489540540638885 20.690699104514543 34-35 21.28776221651617 26.964406631035576 31.428322967716255 20.319508184731998 36-37 21.28131926873143 26.79418011202897 31.428825107705116 20.495675511534486 38-39 20.90582805922527 27.178207841584616 31.469194502506536 20.44676959668357 40-41 21.12628549524189 27.053749606348777 31.313224115851625 20.50674078255771 42-43 21.001281132781898 27.167328457921464 30.91401419543151 20.917376213865126 44-45 21.25035794216819 27.236097016790804 30.63035298646569 20.88319205457531 46-47 21.56546832534033 27.616465281503512 30.028630004178886 20.789436388977276 48-49 21.465356780487575 27.77195696434806 29.758890971606057 21.00379528355831 50-51 21.418824778573008 27.93256815286532 29.629007673030717 21.01959939553096 52-53 21.613139379314635 28.144769823628657 29.236898225079855 21.00519257197685 54-55 21.530289446068892 28.011624708363474 29.193853328113107 21.26423251745453 56-57 21.578976190333243 28.311909891097127 28.972033179363997 21.137080739205636 58-59 21.7662447764018 27.957188780884497 29.210724999954518 21.065841442759186 60-61 21.499907488585258 28.112117649049353 29.476520020761853 20.91145484160354 62-63 21.336070602327744 27.962261638184177 29.624271988088367 21.07739577139971 64-65 21.39424821852105 28.091688517667972 29.33223352133108 21.181829742479895 66-67 21.42914580982637 28.096196956735785 28.990604734055502 21.484052499382344 68-69 21.43622020427611 28.06749822347243 29.138617907034032 21.357663665217427 70-71 21.632289054658575 27.968131128620367 29.084665948289583 21.314913868431475 72-73 21.54800936853362 27.867930892328445 29.04094421778311 21.54311552135482 74-75 21.326880488409675 28.493875503779815 28.64287407510365 21.53636993270686 76-77 21.43037510883807 28.236370271752243 28.770782022224978 21.56247259718471 78-79 21.466494658273405 28.262439301455743 28.680659116466067 21.59040692380478 80-81 21.497511109995276 28.301847660392454 28.511030491972623 21.689610737639647 82-83 21.646286824767444 28.257637687427255 28.67969300238219 21.416382485423107 84-85 21.435060111349593 28.328520774480236 28.480184150194777 21.7562349639754 86-87 21.32055774865797 28.43115815334995 28.51100858978926 21.737275508202817 88-89 21.499057978871654 28.327310316823283 28.361785597592153 21.81184610671291 90-91 21.376177416347154 28.24567579491238 28.541672920447365 21.836473868293105 92-93 21.492544970684186 28.34544849884258 28.441979284995412 21.720027245477823 94-95 21.340430200094676 28.270281539935432 28.338176847263576 22.05111141270632 96-97 21.273362804826466 28.298036735726797 28.378449921233283 22.050150538213455 98-99 21.18160828449884 28.349313133308847 28.084077744405793 22.38500083778652 100-101 21.198929246120574 28.430192872507952 28.050254989913924 22.320622891457546 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1081.0 1 798.0 2 596.5 3 723.5 4 670.0 5 634.5 6 1097.5 7 2484.0 8 4197.0 9 5046.5 10 4586.0 11 3872.0 12 3920.0 13 4203.0 14 4498.5 15 4801.5 16 5219.5 17 5694.5 18 6125.0 19 6701.5 20 7484.0 21 8551.5 22 9961.0 23 12104.5 24 14533.0 25 17570.0 26 22561.0 27 27973.5 28 31671.0 29 35965.5 30 41606.5 31 46800.5 32 53341.0 33 61055.0 34 68509.5 35 75090.0 36 83242.0 37 89629.5 38 90537.0 39 90462.0 40 90515.5 41 91278.0 42 90962.0 43 91318.5 44 93804.0 45 96228.5 46 97264.5 47 99208.5 48 106927.0 49 105712.0 50 94449.5 51 86559.5 52 82127.5 53 79178.5 54 75041.0 55 67984.0 56 59946.5 57 53182.5 58 48218.5 59 43088.0 60 35719.5 61 28554.5 62 21927.5 63 16040.0 64 11273.0 65 7682.0 66 5410.5 67 4062.5 68 3015.5 69 2216.5 70 1552.0 71 963.5 72 623.5 73 453.5 74 317.0 75 198.0 76 126.0 77 67.5 78 36.0 79 24.0 80 13.0 81 7.5 82 5.5 83 4.0 84 2.0 85 1.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02434188671089443 2 0.005021196361888538 3 1.4554192353300109E-4 4 1.8192740441625135E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 7.277096176650054E-5 10-11 0.0100605854642187 12-13 0.0 14-15 0.0 16-17 0.0 18-19 3.638548088325027E-4 20-21 6.7313139634013E-4 22-23 0.0018556595250457638 24-25 0.010260705609076578 26-27 0.005239509247188039 28-29 2.3650562574112675E-4 30-31 0.002528790921385894 32-33 0.004329872225106782 34-35 0.0 36-37 0.008022998534756685 38-39 0.01211636513412234 40-41 0.001473611975771636 42-43 0.0 44-45 0.0 46-47 3.0927658750762733E-4 48-49 0.002528790921385894 50-51 0.0055305930942540415 52-53 0.0 54-55 0.0023650562574112676 56-57 0.0027107183258021452 58-59 1.4554192353300109E-4 60-61 0.0014372264948883857 62-63 0.00403878837804078 64-65 1.8192740441625136E-5 66-67 0.0018192740441625136 68-69 3.638548088325027E-4 70-71 8.914442816396317E-4 72-73 0.0 74-75 1.8192740441625136E-5 76-77 0.0 78-79 0.001218913609588884 80-81 0.0012916845713553846 82-83 0.009078177480370942 84-85 0.005730713239111918 86-87 0.0031291513559595237 88-89 1.0915644264975082E-4 90-91 4.184330301573781E-4 92-93 1.0915644264975082E-4 94-95 0.0 96-97 0.0015827684184213868 98-99 0.0012916845713553846 100-101 1.8192740441625135E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2748349.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.765827849890606 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34048331604959 36.000840392844076 2 15.124177652742954 15.053344428751366 3 5.425078643642188 8.099505895548454 4 2.5169379582429126 5.010300045550877 5 1.2668587021148148 3.1523136039790853 6 0.7737505514038898 2.3103802043954347 7 0.5140418120303732 1.7907201427604564 8 0.34274647550764137 1.364564967701602 9 0.24541969500649952 1.09921628633995 >10 1.2602449664665067 11.62943435338007 >50 0.11581849839885858 3.993006980906201 >100 0.06887324290390305 6.383105399761499 >500 0.0037367468420234605 1.2052544835540715 >1k 0.001538660464342017 1.1641604615483652 >5k 1.4653909184209684E-4 0.5653983185632521 >10k+ 1.4653909184209684E-4 1.1784540344154355 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17684 0.6434408439393978 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14635 0.5325015127263677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9818 0.3572326513117512 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5688 0.20696061526392753 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3818 0.13891976601224953 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.277096176650054E-5 0.0 14-15 0.0 0.0 0.0 7.277096176650054E-5 0.0 16-17 0.0 0.0 0.0 1.4554192353300109E-4 0.0 18-19 0.0 0.0 0.0 2.3650562574112675E-4 0.0 20-21 0.0 0.0 0.0 2.72891106624377E-4 0.0 22-23 0.0 0.0 0.0 4.912039919238786E-4 0.0 24-25 0.0 0.0 0.0 7.277096176650055E-4 0.0 26-27 0.0 0.0 0.0 9.642152434061321E-4 0.0 28-29 0.0 0.0 0.0 0.001964815967695515 0.0 30-31 0.0 0.0 0.0 0.0049302326596804115 0.0 32-33 0.0 0.0 0.0 0.009987814502452199 0.0 34-35 0.0 0.0 0.0 0.017992620296767257 0.0 36-37 0.0 0.0 0.0 0.028562602493351463 0.0 38-39 0.0 0.0 0.0 0.045008839852580584 0.0 40-41 0.0 0.0 0.0 0.0636927842861296 0.0 42-43 0.0 0.0 0.0 0.084396122908699 0.0 44-45 0.0 0.0 0.0 0.11083017477038032 0.0 46-47 0.0 0.0 0.0 0.14310409631382331 0.0 48-49 0.0 0.0 0.0 0.17757933945070295 0.0 50-51 0.0 0.0 0.0 0.21614794918694824 0.0 52-53 0.0 0.0 0.0 0.2582641433093104 0.0 54-55 0.0 0.0 0.0 0.31038634467456644 0.0 56-57 0.0 0.0 0.0 0.3702040752466299 0.0 58-59 0.0 0.0 0.0 0.4390090195968561 0.0 60-61 3.638548088325027E-5 0.0 0.0 0.51618262455023 0.0 62-63 3.638548088325027E-5 0.0 0.0 0.5945205648918678 0.0 64-65 3.638548088325027E-5 0.0 0.0 0.679244157128516 0.0 66-67 3.638548088325027E-5 0.0 0.0 0.7850349427965662 0.0 68-69 3.638548088325027E-5 0.0 0.0 0.9036698032164037 0.0 70-71 3.638548088325027E-5 0.0 0.0 1.0271439325937135 0.0 72-73 3.638548088325027E-5 0.0 0.0 1.1646810503323994 0.0 74-75 3.638548088325027E-5 0.0 0.0 1.314953086380223 0.0 76-77 3.638548088325027E-5 0.0 0.0 1.4754858280371232 0.0 78-79 3.638548088325027E-5 0.0 0.0 1.6617067191975983 0.0 80-81 3.638548088325027E-5 0.0 0.0 1.8682852869122517 0.0 82-83 3.638548088325027E-5 0.0 0.0 2.077974813242423 0.0 84-85 3.638548088325027E-5 0.0 0.0 2.3002719086986403 0.0 86-87 3.638548088325027E-5 0.0 0.0 2.548038840773133 0.0 88-89 3.638548088325027E-5 0.0 0.0 2.8219487408622412 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15960 0.0 30.153477 1 GTATCAA 22755 0.0 27.663036 1 ATCAACG 27740 0.0 22.548704 3 TATCAAC 28025 0.0 22.440067 2 GAGTACT 15860 0.0 22.294811 12-13 TCAACGC 28060 0.0 22.291557 4 CAACGCA 28340 0.0 22.025448 5 AACGCAG 28940 0.0 21.601633 6 TACATGG 15525 0.0 21.569464 2 GTACATG 15835 0.0 21.451063 1 GTACTTT 16875 0.0 20.84143 14-15 AGTACTT 16500 0.0 20.696041 12-13 ACATGGG 15710 0.0 20.406563 3 AGAGTAC 25160 0.0 20.10797 10-11 ACGCAGA 32355 0.0 19.233534 7 CGCAGAG 32460 0.0 19.068878 8 CATGGGG 6285 0.0 18.362986 4 GCAGAGT 33585 0.0 18.118948 9 ACTTTTT 19720 0.0 18.063938 16-17 TACTTTT 19760 0.0 17.377913 14-15 >>END_MODULE