FastQCFastQC Report
Thu 26 May 2016
SRR936760_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936760_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718775
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189090.695497052900663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136980.5038298498404613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88500.32551424814484464No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT43580.1602927789169755No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33450.12303335141745823No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT31330.11523572197037268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA211300.030.1661661
GGTATCA142000.030.05931
ATCAACG250450.025.320113
TCAACGC251700.025.1943664
CAACGCA252850.025.0600555
TATCAAC253650.025.0189572
AACGCAG259250.024.4928076
GTACATG160550.024.2769641
TACATGG156400.024.163132
GAGTACT129050.023.42038212-13
ACATGGG158800.022.780593
ACGCAGA285700.022.1209777
CGCAGAG286300.021.9086448
GTACTTT139650.021.55681214-15
AGTACTT134650.021.52872812-13
CATGGGA93750.020.8401764
GCAGAGT295450.020.7790379
CTATCCG1150.003707848520.6682174
AGAGTAC228200.020.28029310-11
GTGTAGC18800.018.962441
Produced by FastQC (version 0.11.5)