##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936760_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718775 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08076505043632 33.0 31.0 34.0 31.0 34.0 2 32.178837895743484 33.0 31.0 34.0 31.0 34.0 3 32.207227151934234 34.0 31.0 34.0 30.0 34.0 4 35.81107484069112 37.0 35.0 37.0 35.0 37.0 5 35.826111024266446 37.0 35.0 37.0 35.0 37.0 6 35.90662559424741 37.0 35.0 37.0 35.0 37.0 7 35.87083079695819 37.0 35.0 37.0 35.0 37.0 8 35.850190618936836 37.0 35.0 37.0 35.0 37.0 9 37.526195069470624 39.0 37.0 39.0 35.0 39.0 10-11 37.4170271537733 39.0 37.0 39.0 35.0 39.0 12-13 37.34518983733483 39.0 37.0 39.0 35.0 39.0 14-15 38.53889214811818 40.0 38.0 41.0 34.0 41.0 16-17 38.478770218204886 40.0 38.0 41.0 34.0 41.0 18-19 38.486657593953154 40.0 38.0 41.0 34.0 41.0 20-21 38.43820948772885 40.0 38.0 41.0 34.0 41.0 22-23 38.33461246333367 40.0 38.0 41.0 34.0 41.0 24-25 38.3047501172403 40.0 38.0 41.0 34.0 41.0 26-27 38.32673685759211 40.0 38.0 41.0 34.0 41.0 28-29 38.26797804158123 40.0 38.0 41.0 34.0 41.0 30-31 38.09804305247768 40.0 38.0 41.0 34.0 41.0 32-33 37.88802512160808 40.0 37.0 41.0 33.5 41.0 34-35 37.72455536041048 40.0 37.0 41.0 33.0 41.0 36-37 37.552166876626416 40.0 37.0 41.0 33.0 41.0 38-39 37.395104596739344 40.0 37.0 41.0 33.0 41.0 40-41 37.22541806512124 40.0 36.5 41.0 32.0 41.0 42-43 36.99772029682485 39.5 36.0 41.0 31.5 41.0 44-45 36.70770199814255 39.0 36.0 41.0 31.0 41.0 46-47 36.316951384355086 39.0 36.0 41.0 30.0 41.0 48-49 36.011043208798085 39.0 35.5 41.0 29.0 41.0 50-51 35.30701639525154 38.5 35.0 40.0 28.0 40.5 52-53 35.49384024054952 38.5 35.5 40.0 28.5 40.5 54-55 35.89355426616767 39.0 36.0 41.0 29.0 41.0 56-57 35.789299776553776 39.0 35.0 41.0 28.5 41.0 58-59 35.600728453071696 39.0 35.0 41.0 28.0 41.0 60-61 35.336355711671615 39.0 35.0 40.5 28.0 41.0 62-63 35.05329109617383 38.0 35.0 40.0 27.0 41.0 64-65 34.74204430304089 38.0 34.5 40.0 26.0 41.0 66-67 34.38190398249212 37.0 34.0 40.0 26.0 41.0 68-69 33.98077994685106 37.0 34.0 39.5 26.0 41.0 70-71 33.57286903108937 36.0 34.0 39.0 25.0 41.0 72-73 33.188081764765386 36.0 33.5 39.0 24.5 40.0 74-75 32.79807633952791 35.0 33.0 38.0 23.5 40.0 76-77 32.353675460455534 35.0 33.0 37.0 22.0 39.0 78-79 31.90343095695672 35.0 32.0 37.0 20.5 39.0 80-81 31.478657851422057 35.0 32.0 36.0 19.5 38.5 82-83 31.08185561512078 35.0 31.5 36.0 18.0 37.0 84-85 30.716539985839212 35.0 31.0 35.0 16.0 37.0 86-87 30.383328153304337 34.0 31.0 35.0 10.0 36.5 88-89 30.034499360925416 34.0 31.0 35.0 7.0 36.0 90-91 29.691745363261028 34.0 30.5 35.0 2.0 36.0 92-93 29.342805307537404 34.0 30.0 35.0 2.0 35.5 94-95 28.999020146941177 34.0 30.0 35.0 2.0 35.0 96-97 28.55245248319556 34.0 29.0 35.0 2.0 35.0 98-99 28.045683441991336 34.0 29.0 35.0 2.0 35.0 100-101 25.851633916009966 32.0 24.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18026100945732537 1101 2 0.14491937473638217 1101 3 0.10539180345445232 1101 4 0.15159120698561424 1101 5 0.13178635819689077 1101 6 0.10842385030759516 1101 7 0.1300872431501361 1101 8 0.14163756445546483 1101 9 0.07435585940640266 1101 10-11 0.12777857646878488 1101 12-13 0.2367565952449624 1101 14-15 0.24900262974557563 1101 16-17 0.30784760467147265 1101 18-19 0.25015788261074334 1101 20-21 0.32508648548283503 1101 22-23 0.3533070928498816 1101 24-25 0.33867685983283735 1101 26-27 0.271378846373068 1101 28-29 0.2209730594708219 1101 30-31 0.17998483654583453 1101 32-33 0.08488722066180543 1101 34-35 0.2053886198976329 1101 36-37 0.23061462518406017 1101 38-39 0.1455362557481621 1101 40-41 0.16799241227809603 1101 42-43 0.10186144108688922 1101 44-45 0.2727146076336666 1101 46-47 0.2657290741734286 1101 48-49 0.22631973724622867 1101 50-51 0.07891062478480393 1101 52-53 0.0763224522255328 1101 54-55 0.208298831786486 1101 56-57 0.07717154606546472 1101 58-59 0.17321977541890732 1101 60-61 0.19348634176396473 1101 62-63 0.20859874659517885 1101 64-65 0.04973502309232458 1101 66-67 0.031773876471959284 1101 68-69 0.12121224021792187 1101 70-71 2.5836153416491925E-4 1101 72-73 0.04086646321222531 1101 74-75 0.060434156418367024 1101 76-77 0.10966967600668198 1101 78-79 0.060349354794510646 1101 80-81 0.1046173562695536 1101 82-83 0.09799160367027682 1101 84-85 0.04033978341521305 1101 86-87 0.15345088824128794 1101 88-89 0.030443446074610137 1101 90-91 0.1327585429629039 1101 92-93 0.1900493401420782 1101 94-95 0.2685638711521392 1101 96-97 0.38925648938371893 1101 98-99 0.31943062947241785 1101 100-101 0.4380922885981917 1102 1 0.21685389575945635 1102 2 0.19054253499007245 1102 3 0.24936944873934408 1102 4 0.1958150133301757 1102 5 0.2404889270524535 1102 6 0.12986847571831817 1102 7 0.06068598751758714 1102 8 0.0640462296742399 1102 9 0.07916841369325311 1102 10-11 0.16671840206647204 1102 12-13 0.1154587490996235 1102 14-15 0.05552302194326586 1102 16-17 0.1403849128882726 1102 18-19 -0.0031568460624029626 1102 20-21 0.07182126568889657 1102 22-23 0.06813813287853776 1102 24-25 -0.008279909450692458 1102 26-27 0.05107707519291438 1102 28-29 -0.04746626776175589 1102 30-31 -0.06722937890953773 1102 32-33 2.7836628998301194E-4 1102 34-35 0.030080954976639873 1102 36-37 0.07704427051846352 1102 38-39 0.12215386736702527 1102 40-41 0.05835281507651757 1102 42-43 0.11678290762725396 1102 44-45 0.19763220811302773 1102 46-47 0.2018939270920086 1102 48-49 0.33702850781698857 1102 50-51 0.22883042768122408 1102 52-53 0.3705515370903143 1102 54-55 0.2713887974287772 1102 56-57 0.5058288871037391 1102 58-59 0.26424039368287566 1102 60-61 0.3201449802260967 1102 62-63 0.19480215091308395 1102 64-65 0.44224760599239943 1102 66-67 0.1455343597951071 1102 68-69 0.46117842856571656 1102 70-71 0.3474499339580106 1102 72-73 0.2679545912014163 1102 74-75 0.49495538735471456 1102 76-77 0.4160222476653246 1102 78-79 0.35317653749902433 1102 80-81 0.4529949575789125 1102 82-83 0.4569571884465873 1102 84-85 0.5021010909155947 1102 86-87 0.3309875448338673 1102 88-89 0.4878719985733184 1102 90-91 0.1613914207902809 1102 92-93 0.4523994392690476 1102 94-95 0.4218266155021233 1102 96-97 0.23949868245232864 1102 98-99 0.344037998577857 1102 100-101 0.26751090146132483 1103 1 0.19488663606018974 1103 2 0.2890541221871388 1103 3 0.30968022402596773 1103 4 0.24405382332845704 1103 5 0.14857761774984368 1103 6 0.17017641762809177 1103 7 0.1932204493172378 1103 8 0.18166541492627886 1103 9 0.1144697228313305 1103 10-11 0.21251805541761826 1103 12-13 0.16589632369178986 1103 14-15 0.3651999869723852 1103 16-17 0.3925726306239241 1103 18-19 0.4341832526049316 1103 20-21 0.31889032981872845 1103 22-23 0.37042865624712107 1103 24-25 0.33562229206656724 1103 26-27 0.3488161377425527 1103 28-29 0.37079629479269016 1103 30-31 0.34384641330024834 1103 32-33 0.37944658908902085 1103 34-35 0.3421944895666513 1103 36-37 0.35529372585920527 1103 38-39 0.4025812353865561 1103 40-41 0.4052065715412567 1103 42-43 0.45134546911582873 1103 44-45 0.36622232165610313 1103 46-47 0.4449914516540687 1103 48-49 0.5046457217093092 1103 50-51 0.5024485329799759 1103 52-53 0.5440083220395948 1103 54-55 0.6584266778559353 1103 56-57 0.6959169111607437 1103 58-59 0.742104921636404 1103 60-61 0.6758602831826934 1103 62-63 0.7125001491602774 1103 64-65 0.7250348623367415 1103 66-67 0.6654286907751299 1103 68-69 0.6755268020801211 1103 70-71 0.6108760060543048 1103 72-73 0.4512336629879883 1103 74-75 0.4286669940894612 1103 76-77 0.5606482848669998 1103 78-79 0.6837151967943527 1103 80-81 0.5790781059097121 1103 82-83 0.581036224552065 1103 84-85 0.559301308934522 1103 86-87 0.5749776141481178 1103 88-89 0.4816547129255717 1103 90-91 0.5858502101104968 1103 92-93 0.5013199982328338 1103 94-95 0.6214358930417312 1103 96-97 0.6581245236563547 1103 98-99 0.5235474270066867 1103 100-101 0.7955326156005071 1104 1 0.046965753482531625 1104 2 0.027261794440768483 1104 3 -0.010674940061015548 1104 4 0.1270743091339952 1104 5 0.09949757717765806 1104 6 -0.05336123097500689 1104 7 -0.01238020716328947 1104 8 -0.007065278894586413 1104 9 0.06534462276817976 1104 10-11 0.07629777603634835 1104 12-13 -2.824056595684965E-4 1104 14-15 -0.12243610304881258 1104 16-17 0.03410879050287008 1104 18-19 0.09626503837781542 1104 20-21 0.06082986018903114 1104 22-23 -0.16612334385563798 1104 24-25 -0.21205334257823694 1104 26-27 -0.19826740483255634 1104 28-29 -0.14071029362997933 1104 30-31 0.013098614275115494 1104 32-33 -0.21992574773968698 1104 34-35 -0.18828913653943147 1104 36-37 -0.20557506877559462 1104 38-39 -0.15093877061308802 1104 40-41 -0.20629748940652348 1104 42-43 -0.09223917809011084 1104 44-45 -0.1329935396161801 1104 46-47 -0.18490527199265472 1104 48-49 -0.07316621841356863 1104 50-51 -0.17807755263263658 1104 52-53 -0.11505333840368337 1104 54-55 0.015624864206237987 1104 56-57 0.07087972413257404 1104 58-59 -0.0644563410403336 1104 60-61 -0.130308599375077 1104 62-63 -0.28257096403910253 1104 64-65 -0.11698987708474817 1104 66-67 -0.2559644575304745 1104 68-69 -0.2468431013289063 1104 70-71 -0.15345317103301426 1104 72-73 -0.1016901593330175 1104 74-75 -0.14513553181094352 1104 76-77 -0.06784594917552056 1104 78-79 0.06120822966478556 1104 80-81 -0.045281842148607865 1104 82-83 -0.12201825993420812 1104 84-85 -0.08129088050510802 1104 86-87 0.09182100724587272 1104 88-89 0.031553023495057175 1104 90-91 0.04636002044215104 1104 92-93 -0.04209539102316384 1104 94-95 0.05129913800459818 1104 96-97 0.06289424168959812 1104 98-99 0.033517020655018825 1104 100-101 0.17366580399765752 1105 1 0.11924071298252414 1105 2 0.168648281216619 1105 3 0.21351409448553227 1105 4 0.19319561520872952 1105 5 0.13063453477155917 1105 6 0.0937528474063285 1105 7 0.07900511682798594 1105 8 0.07711198739448832 1105 9 0.15876854945220487 1105 10-11 0.19468432906897704 1105 12-13 0.17405997384523886 1105 14-15 0.23989283598265843 1105 16-17 0.26779947532593695 1105 18-19 0.2061211953440747 1105 20-21 0.25074406423885875 1105 22-23 0.2723918438660533 1105 24-25 0.19687122648940658 1105 26-27 0.26928564008340317 1105 28-29 0.2781584420326695 1105 30-31 0.30875211456383767 1105 32-33 0.1413302482796439 1105 34-35 0.01390440171311269 1105 36-37 0.117268064564648 1105 38-39 0.3327468133153886 1105 40-41 0.3986007625552901 1105 42-43 0.524858274665732 1105 44-45 0.5022121986598762 1105 46-47 0.35962833511428727 1105 48-49 0.42299494703883056 1105 50-51 0.3083566456076525 1105 52-53 0.5142384336449055 1105 54-55 0.42082358628405103 1105 56-57 0.5207967276411054 1105 58-59 0.4675269262702315 1105 60-61 0.5767606262367266 1105 62-63 0.4932969339798632 1105 64-65 0.4237806410169611 1105 66-67 0.47305594692549846 1105 68-69 0.3836001016906536 1105 70-71 0.2587526993178173 1105 72-73 0.427054917480973 1105 74-75 0.5879315275112518 1105 76-77 0.5744989573015005 1105 78-79 0.520078838946354 1105 80-81 0.3149039532750244 1105 82-83 0.465614944717629 1105 84-85 0.5118467927497932 1105 86-87 0.5164984003442861 1105 88-89 0.458782728735752 1105 90-91 0.5254284171860029 1105 92-93 0.5215813250274799 1105 94-95 0.6608779269710432 1105 96-97 0.5818340278776475 1105 98-99 0.7954448319170879 1105 100-101 0.7324134652649086 1106 1 0.2718367332880618 1106 2 0.2957378602597629 1106 3 0.3388725543294129 1106 4 0.29539330689016197 1106 5 0.2445106661828902 1106 6 0.2899820749337465 1106 7 0.3253067786257944 1106 8 0.2557077203574707 1106 9 0.27755105507932853 1106 10-11 0.34406516571473134 1106 12-13 0.372353159037516 1106 14-15 0.3910754900701079 1106 16-17 0.5261020590144625 1106 18-19 0.5595597279814086 1106 20-21 0.3819115729788578 1106 22-23 0.3758886232348644 1106 24-25 0.32166206505078065 1106 26-27 0.2429040611360378 1106 28-29 0.1597337976190829 1106 30-31 0.3299048969976255 1106 32-33 0.26695252451163043 1106 34-35 0.4163546555323734 1106 36-37 0.31640885044654965 1106 38-39 0.10746156596133005 1106 40-41 0.3345701246548103 1106 42-43 0.2859713679083953 1106 44-45 0.3733938949387934 1106 46-47 0.4793804422930563 1106 48-49 0.4711634375325815 1106 50-51 0.4918906597832162 1106 52-53 0.48272994506677236 1106 54-55 0.6632725829796158 1106 56-57 0.6258881384930746 1106 58-59 0.7373022603059596 1106 60-61 0.6037985434820641 1106 62-63 0.6769176298277557 1106 64-65 0.5138898910528766 1106 66-67 0.47631770402524864 1106 68-69 0.6095864050286153 1106 70-71 0.5885438371943579 1106 72-73 0.5588118975106653 1106 74-75 0.7844764726767224 1106 76-77 0.6860087628663649 1106 78-79 0.5307856417814634 1106 80-81 0.6639973276344868 1106 82-83 0.5994710818758122 1106 84-85 0.6326274067050655 1106 86-87 0.7705981201852907 1106 88-89 0.8089679449610259 1106 90-91 0.7537945754159736 1106 92-93 0.8136927540778096 1106 94-95 1.0685551215498492 1106 96-97 1.0753175775160742 1106 98-99 1.0088479855016885 1106 100-101 1.1812882796895074 1107 1 0.2012385094051723 1107 2 0.32726381101986135 1107 3 0.21014398801933254 1107 4 0.20283917882546376 1107 5 0.2088343852799852 1107 6 0.15583340830120562 1107 7 0.2237341216160189 1107 8 0.2452364351038483 1107 9 0.29046718856962883 1107 10-11 0.26653733390840983 1107 12-13 0.29664450366565376 1107 14-15 0.2885999308747387 1107 16-17 0.4243936979559564 1107 18-19 0.3723738754963719 1107 20-21 0.46199813949367297 1107 22-23 0.44255309321490444 1107 24-25 0.5044906723088047 1107 26-27 0.507410062569214 1107 28-29 0.473063633315455 1107 30-31 0.5110303896525892 1107 32-33 0.4597396079942442 1107 34-35 0.36707463557024766 1107 36-37 0.3524533429479533 1107 38-39 0.39504826043847885 1107 40-41 0.43782055972672396 1107 42-43 0.5468843791178912 1107 44-45 0.49643402392482017 1107 46-47 0.5687112632993561 1107 48-49 0.5935360510351657 1107 50-51 0.49308563598221866 1107 52-53 0.5649528426457238 1107 54-55 0.6063693992769004 1107 56-57 0.6163813692859321 1107 58-59 0.5503677898140751 1107 60-61 0.6083827298975848 1107 62-63 0.6128840653091601 1107 64-65 0.6901906920970475 1107 66-67 0.7101177372954552 1107 68-69 0.6655499613576339 1107 70-71 0.6272002967326742 1107 72-73 0.661719329562672 1107 74-75 0.6505646643131016 1107 76-77 0.6723313175062842 1107 78-79 0.6797763389202274 1107 80-81 0.734114605108001 1107 82-83 0.788555895306736 1107 84-85 0.789788690423137 1107 86-87 0.7925535764989498 1107 88-89 0.7779244121770788 1107 90-91 0.787016986738081 1107 92-93 0.9191112548711757 1107 94-95 0.9998788918318802 1107 96-97 0.9853544306665079 1107 98-99 1.160810351781059 1107 100-101 1.1604542212363143 1108 1 0.01123976088828016 1108 2 0.11301611703594006 1108 3 0.09485045433436312 1108 4 0.02023399862991937 1108 5 -0.04197649996348218 1108 6 -0.016529619234034953 1108 7 0.08009965311169509 1108 8 -0.02217779076685389 1108 9 -0.10018282827630287 1108 10-11 -0.19341296966012322 1108 12-13 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11 4584.0 12 6814.0 13 9282.0 14 10862.0 15 14778.0 16 20623.0 17 29738.0 18 26193.0 19 13296.0 20 11693.0 21 11516.0 22 12276.0 23 13201.0 24 14868.0 25 17208.0 26 20182.0 27 24071.0 28 29760.0 29 37009.0 30 47222.0 31 61359.0 32 81692.0 33 113235.0 34 162835.0 35 246087.0 36 384092.0 37 594105.0 38 570380.0 39 111700.0 40 40.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.672056956785696 19.625181669334214 14.751513144581194 21.951248229298894 2 14.891869847211273 21.028731079844608 41.14412926539339 22.935269807550736 3 18.961069444270713 26.45146170775263 30.27689706348722 24.310571784489436 4 12.059684070963518 17.44237093603002 39.047635168825565 31.450309824180895 5 12.78188900747458 38.39389486991919 34.36646463739101 14.45775148521522 6 29.217041034052983 37.6567381527036 18.521419116577654 14.604801696665765 7 26.571607944040792 34.252787950870825 21.715215381716696 17.460388723371683 8 25.62279833179347 34.355704920325834 20.72003062550383 19.301466122376862 9 26.566738238651965 16.89811628521522 21.28569735576233 35.24944812037049 10-11 23.92243310770139 27.50790337483629 27.928637664101185 20.641025853361135 12-13 25.4851398713998 25.366631298114207 28.24184434671781 20.90638448376819 14-15 22.761950115815452 26.009475006753142 27.605185234799546 23.62338964263186 16-17 20.644192446716243 29.308596310016327 28.124801063301703 21.922410179965727 18-19 20.510862517048007 27.722676631364806 31.245919701942277 20.52054114964491 20-21 22.008336035098786 26.54189233001602 31.10406096507675 20.345710669808444 22-23 21.333363993414213 26.64835033434818 31.05825108766637 20.960034584571236 24-25 21.04636662926928 26.83224513067628 31.37137444593625 20.75001379411819 26-27 20.846638595574895 26.891143444872856 31.414862107428476 20.84735585212377 28-29 20.637540135237682 27.166622342724487 31.417913714342678 20.77792380769515 30-31 20.953546095638572 26.81540336902259 31.276731998063745 20.95431853727509 32-33 20.749656489132256 26.87364924501176 31.196948008703686 21.1797462571523 34-35 21.18734155610845 26.98019000760678 31.05750400569699 20.77496443058778 36-37 21.156252959876262 26.690042217605964 31.18482409943897 20.968880723078804 38-39 20.8103568007839 27.141617587125687 31.156895387381393 20.891130224709016 40-41 21.03655043278495 27.026993723087156 31.111171190755876 20.825284653372023 42-43 20.863357116951693 27.095411814124365 30.693686416706946 21.347544652216996 44-45 21.05532150924894 27.20283633839718 30.56999506936453 21.171847082989345 46-47 21.337384229459236 27.460036634617506 30.04279555970781 21.159783576215453 48-49 21.24817863375325 27.571425234835097 29.857165162401593 21.323230969010062 50-51 21.140733779435823 27.81479972744652 29.723244195594823 21.321222297522834 52-53 21.32028344282728 28.080566650243032 29.282967774185913 21.316182132743773 54-55 21.169916332074667 27.937454133460367 29.374504549408954 21.518124985056016 56-57 21.295597579127396 28.171749019212598 29.052788625349052 21.479864776310954 58-59 21.446478198970112 27.898259592234293 29.30261753605519 21.352644672740404 60-61 21.29778681468194 27.904459750210435 29.621714709651958 21.17603872545567 62-63 21.054636297208127 27.835586618266518 29.730137334236005 21.37963975028935 64-65 21.080635322085005 27.993624194955302 29.485082201502877 21.440658281456813 66-67 21.106774564874858 28.062872276593577 29.031531788388154 21.79882137014341 68-69 21.14229373146477 28.027966294878055 29.244504905655795 21.585235068001385 70-71 21.34414094672544 27.807695336737225 29.168301265750113 21.679862450787223 72-73 21.182957087423173 27.77399019413292 29.260760328961293 21.78229238948261 74-75 21.050951998989113 28.35540167483841 28.8403172220247 21.753329104147774 76-77 21.158772973454205 28.152915519787392 28.908347515272716 21.779963991485683 78-79 21.17087834546656 28.13611507212978 28.881786034355645 21.811220548048016 80-81 21.166510016212264 28.15758636955164 28.734116481314853 21.941787132921238 82-83 21.286185262214495 28.113773016867537 28.945432056160627 21.65460966475734 84-85 21.31115128389947 28.122210698448008 28.67905483634657 21.88758318130596 86-87 21.031072342092845 28.224775278987007 28.85060019112668 21.89355218779346 88-89 21.273522819724707 28.122310383286596 28.60659317853407 21.99757361845463 90-91 21.187690163368018 28.052760227381025 28.83584051242784 21.923709096823117 92-93 21.29982442910021 28.14580166982773 28.785900459045322 21.76847344202674 94-95 21.168808333649068 28.04889129052157 28.693837568295404 22.088462807533947 96-97 21.151522263492126 28.05194857746359 28.784525122874733 22.012004036169554 98-99 21.072344136187155 28.113868266689725 28.513739121796327 22.300048475326793 100-101 21.118369631969866 28.003022580835722 28.623531336547924 22.255076450646484 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 738.0 1 583.5 2 589.0 3 769.5 4 662.5 5 666.5 6 1230.0 7 2703.0 8 4836.0 9 5762.5 10 4875.0 11 4064.5 12 4054.5 13 4206.0 14 4558.5 15 4776.5 16 4877.0 17 5246.0 18 5662.5 19 6079.0 20 6711.0 21 7795.0 22 9415.0 23 11627.5 24 13668.0 25 16466.5 26 21517.5 27 26932.5 28 30121.5 29 34017.0 30 39861.5 31 45505.5 32 52028.5 33 59290.0 34 66915.0 35 73650.0 36 82085.0 37 88928.5 38 89119.0 39 88790.0 40 89353.5 41 90279.5 42 90554.5 43 91059.5 44 93025.5 45 95258.0 46 96258.5 47 98478.0 48 107385.0 49 105851.5 50 93887.5 51 86714.0 52 82352.0 53 79210.0 54 74887.0 55 67650.5 56 60031.0 57 53796.0 58 48751.5 59 43202.5 60 35776.5 61 28618.5 62 21779.0 63 15797.0 64 10958.0 65 7510.5 66 5413.0 67 4050.5 68 3094.5 69 2254.5 70 1503.5 71 943.5 72 637.5 73 494.0 74 336.5 75 201.5 76 135.5 77 73.0 78 45.0 79 33.0 80 22.0 81 15.0 82 13.0 83 13.5 84 14.5 85 11.0 86 7.0 87 4.0 88 2.5 89 2.5 90 1.5 91 0.5 92 2.0 93 4.0 94 2.5 95 1.5 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03402267565355721 2 0.026041139851587573 3 0.024018169947862548 4 0.04656508905665235 5 0.09202673998400016 6 0.10519443499370121 7 0.05027999742531103 8 0.0766889499866668 9 0.06241781684766118 10-11 0.06398102086417595 12-13 0.061498285073240705 14-15 0.055944313155741096 16-17 0.04421108771413596 18-19 0.05329606164541016 20-21 0.052082279703175145 22-23 0.029425016781454885 24-25 0.008275785969784186 26-27 0.0030344548555875346 28-29 0.005664315730430065 30-31 0.004524096330148689 32-33 0.005756268907872112 34-35 0.005406846833592335 36-37 0.0045057056946602786 38-39 0.0021700949876322976 40-41 0.005940175262756205 42-43 0.003567783284751405 44-45 0.0021517043521438886 46-47 0.0012873444841886511 48-49 2.022969903725023E-4 50-51 0.0015632040165147905 52-53 0.004836737133451646 54-55 0.010666568583277395 56-57 0.006197644159593935 58-59 0.0047999558624748275 60-61 0.0017103291004220652 62-63 0.006933269579130307 64-65 0.002188485623120707 66-67 0.019641198701621135 68-69 0.04216972717492253 70-71 0.05491443756839017 72-73 0.003549392649262995 74-75 0.013369992000073562 76-77 0.019457292346737043 78-79 0.022767606734650715 80-81 0.07372805767303289 82-83 0.026261827477448485 84-85 0.008845895669924874 86-87 0.025103217441678698 88-89 0.042224899081387754 90-91 0.03847320944175226 92-93 0.0393375693097075 94-95 0.009342442828111926 96-97 0.013094132467747422 98-99 0.0330111907016947 100-101 0.06957177405265239 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718775.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.88691788551403 #Duplication Level Percentage of deduplicated Percentage of total 1 72.36191149543441 36.09912736811571 2 15.068541388729322 15.03446173828019 3 5.475812163362495 8.195141752504913 4 2.4966234628801853 4.981953987350061 5 1.2967737541507205 3.234602289470338 6 0.7672818535767486 2.2966396094656956 7 0.4943947473006342 1.7264681113131342 8 0.3426738450124492 1.3675953574119533 9 0.24215241426717798 1.0872213845693115 >10 1.2646049085172206 11.600366032065981 >50 0.11437636862291958 3.958502359476088 >100 0.06940202447391143 6.418568733184432 >500 0.003904505467574918 1.2840291387618799 >1k 0.001326058460674567 1.191708994596481 >5k 7.36699144819204E-5 0.32525210023372775 >10k+ 1.473398289638408E-4 1.1983610432001313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18909 0.695497052900663 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13698 0.5038298498404613 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8850 0.32551424814484464 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 4358 0.1602927789169755 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3345 0.12303335141745823 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3133 0.11523572197037268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.6781270976818606E-5 0.0 16-17 0.0 0.0 0.0 1.6551571939568373E-4 0.0 18-19 0.0 0.0 0.0 2.2068762586091164E-4 0.0 20-21 0.0 0.0 0.0 2.9425016781454885E-4 0.0 22-23 0.0 0.0 0.0 4.413752517218232E-4 0.0 24-25 0.0 0.0 0.0 5.517190646522791E-4 0.0 26-27 0.0 0.0 0.0 8.827505034436464E-4 0.0 28-29 0.0 0.0 0.0 0.001967797997259795 0.0 30-31 0.0 0.0 0.0 0.004579268236613917 0.0 32-33 0.0 0.0 0.0 0.011015990657557172 0.0 34-35 0.0 0.0 0.0 0.01954924552417909 0.0 36-37 0.0 0.0 0.0 0.03279050307583378 0.0 38-39 0.0 0.0 0.0 0.05158573254498809 0.0 40-41 0.0 0.0 0.0 0.07350737004717198 0.0 42-43 0.0 0.0 0.0 0.09916230655350296 0.0 44-45 0.0 0.0 0.0 0.13151143437761492 0.0 46-47 0.0 0.0 0.0 0.17070187860341512 0.0 48-49 0.0 0.0 0.0 0.2123566679846622 0.0 50-51 0.0 0.0 0.0 0.2559976459986575 0.0 52-53 0.0 0.0 0.0 0.3040339858943826 0.0 54-55 0.0 0.0 0.0 0.36111851845040505 0.0 56-57 0.0 0.0 0.0 0.4280604316282149 0.0 58-59 0.0 0.0 0.0 0.5026160678982262 0.0 60-61 0.0 0.0 0.0 0.5878198821160265 0.0 62-63 0.0 0.0 0.0 0.6745133378083881 0.0 64-65 0.0 0.0 0.0 0.7667055935117838 0.0 66-67 0.0 0.0 0.0 0.8799919081203851 0.0 68-69 0.0 0.0 0.0 1.0103447324622303 0.0 70-71 0.0 0.0 0.0 1.142886042427196 0.0 72-73 0.0 0.0 0.0 1.2899743450634937 0.0 74-75 0.0 0.0 0.0 1.4497889674577706 0.0 76-77 0.0 0.0 0.0 1.623506910281285 0.0 78-79 0.0 0.0 0.0 1.8224935862658733 0.0 80-81 0.0 0.0 0.0 2.041378929848921 0.0 82-83 0.0 0.0 0.0 2.2641998694264878 0.0 84-85 0.0 0.0 0.0 2.500942520068781 0.0 86-87 0.0 0.0 0.0 2.759128651690559 0.0 88-89 0.0 0.0 0.0 3.0421605318571783 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 21130 0.0 30.166166 1 GGTATCA 14200 0.0 30.0593 1 ATCAACG 25045 0.0 25.32011 3 TCAACGC 25170 0.0 25.194366 4 CAACGCA 25285 0.0 25.060055 5 TATCAAC 25365 0.0 25.018957 2 AACGCAG 25925 0.0 24.492807 6 GTACATG 16055 0.0 24.276964 1 TACATGG 15640 0.0 24.16313 2 GAGTACT 12905 0.0 23.420382 12-13 ACATGGG 15880 0.0 22.78059 3 ACGCAGA 28570 0.0 22.120977 7 CGCAGAG 28630 0.0 21.908644 8 GTACTTT 13965 0.0 21.556812 14-15 AGTACTT 13465 0.0 21.528728 12-13 CATGGGA 9375 0.0 20.840176 4 GCAGAGT 29545 0.0 20.779037 9 CTATCCG 115 0.0037078485 20.668217 4 AGAGTAC 22820 0.0 20.280293 10-11 GTGTAGC 1880 0.0 18.96244 1 >>END_MODULE