FastQCFastQC Report
Thu 26 May 2016
SRR936760_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936760_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2718775
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171790.6318654541107668No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141760.5214112973673806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95410.3509301063898263No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58170.21395665327215382No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38390.14120329928000663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA161900.028.6113661
GTATCAA231600.027.3446541
ATCAACG281350.022.437323
GTACATG152500.022.337271
TCAACGC282800.022.3222794
TATCAAC282700.022.297392
TACATGG151550.022.0661032
CAACGCA286200.022.0404975
GAGTACT157350.021.871612-13
AACGCAG292400.021.5905916
AGTACTT162950.021.3531612-13
ACATGGG153800.021.0321523
GTACTTT164250.020.77812614-15
AGAGTAC250550.019.8981610-11
ACGCAGA325300.019.333967
CGCAGAG326350.019.1847728
CATGGGG62350.018.740954
GCAGAGT335300.018.2193269
CATGGGA88850.018.1766744
ACTTTTT190950.018.03446616-17
Produced by FastQC (version 0.11.5)