##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936760_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2718775 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.59654108927734 31.0 31.0 34.0 30.0 34.0 2 31.73702421127162 31.0 31.0 34.0 30.0 34.0 3 31.847733997848295 31.0 31.0 34.0 30.0 34.0 4 35.63366001232173 37.0 35.0 37.0 33.0 37.0 5 35.42804755818337 37.0 35.0 37.0 33.0 37.0 6 35.537221358884054 37.0 35.0 37.0 33.0 37.0 7 35.48282369817289 37.0 35.0 37.0 33.0 37.0 8 35.431151897453816 37.0 35.0 37.0 33.0 37.0 9 37.010668775459536 39.0 37.0 39.0 33.0 39.0 10-11 36.88868111557595 39.0 37.0 39.0 33.0 39.0 12-13 36.75998179327087 39.0 37.0 39.0 32.0 39.0 14-15 37.73690779854898 39.0 37.0 41.0 32.5 41.0 16-17 37.729285064045385 39.0 36.5 41.0 32.5 41.0 18-19 37.92197460253239 39.5 37.0 41.0 33.0 41.0 20-21 37.93638403325026 40.0 37.0 41.0 33.0 41.0 22-23 37.819932874180466 40.0 37.0 41.0 32.0 41.0 24-25 37.703254774668736 39.0 37.0 41.0 32.0 41.0 26-27 37.440619948322315 39.0 36.0 41.0 32.0 41.0 28-29 37.25190407444529 39.0 36.0 40.5 31.5 41.0 30-31 37.17582459011871 39.0 36.0 40.0 31.0 41.0 32-33 36.99025498616105 39.0 35.5 40.0 31.0 41.0 34-35 36.81237432299473 39.0 35.0 40.0 31.0 41.0 36-37 36.60392676848949 39.0 35.0 40.0 30.0 41.0 38-39 36.388911366332266 38.0 35.0 40.0 30.0 41.0 40-41 36.02734540372043 38.0 35.0 40.0 29.5 41.0 42-43 35.74681115575949 38.0 35.0 40.0 29.0 41.0 44-45 35.425342479609384 38.0 34.5 40.0 27.5 41.0 46-47 34.94833187740802 38.0 34.0 40.0 25.5 41.0 48-49 34.48066518928562 38.0 33.5 40.0 24.0 41.0 50-51 34.13964487682872 38.0 33.0 40.0 23.5 41.0 52-53 33.83493098914033 38.0 33.0 40.0 23.0 41.0 54-55 33.40736600123218 37.0 32.5 40.0 21.0 41.0 56-57 33.161789224926665 37.0 32.0 39.5 20.0 40.5 58-59 32.799139502165495 36.5 31.5 39.0 19.0 40.0 60-61 32.359754668922584 36.0 31.0 39.0 18.0 40.0 62-63 31.943949756783844 36.0 31.0 39.0 15.0 40.0 64-65 31.56863863320797 35.0 30.0 38.0 13.0 40.0 66-67 31.158950446432677 35.0 29.5 38.0 9.0 40.0 68-69 30.63740452961352 34.5 29.0 38.0 8.0 40.0 70-71 30.142972662320346 34.0 29.0 37.0 7.0 39.0 72-73 29.666947798181166 34.0 28.0 36.5 6.0 39.0 74-75 29.11749078169396 33.5 27.5 36.0 2.0 39.0 76-77 27.877257956248677 31.5 26.0 34.5 2.0 36.5 78-79 28.549896184862668 33.0 27.0 35.0 2.0 37.0 80-81 28.371753639046997 33.0 27.0 35.0 2.0 37.0 82-83 27.941407251427574 33.0 26.0 35.0 2.0 36.5 84-85 27.37835587718734 32.5 26.0 35.0 2.0 36.0 86-87 26.89222186462653 32.0 25.5 34.5 2.0 35.5 88-89 26.36029112375978 32.0 25.0 34.0 2.0 35.0 90-91 25.717627240209286 31.5 23.5 34.0 2.0 35.0 92-93 25.025569603957663 31.0 20.0 34.0 2.0 35.0 94-95 24.214715818705116 31.0 15.5 34.0 2.0 35.0 96-97 23.12930437421265 30.0 2.0 34.0 2.0 35.0 98-99 21.895705050988035 29.5 2.0 34.0 2.0 35.0 100-101 18.55768700977462 24.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1991081709863849 1101 2 0.29232858765695013 1101 3 0.26584769019416044 1101 4 0.1452489852075871 1101 5 -0.02342546826470482 1101 6 0.07548264427402529 1101 7 5.770793817561071E-5 1101 8 0.13778328939436335 1101 9 0.17621202340477993 1101 10-11 0.22912803517354519 1101 12-13 0.22898339727542094 1101 14-15 0.4190548952417572 1101 16-17 0.45134479021860585 1101 18-19 0.3067637173955404 1101 20-21 0.1589629166142572 1101 22-23 0.28342895887712416 1101 24-25 0.23680061286155762 1101 26-27 0.2488454455203737 1101 28-29 0.29830390903854465 1101 30-31 0.3991242715844052 1101 32-33 0.3695516597162296 1101 34-35 0.07167449435685569 1101 36-37 0.18620283491989653 1101 38-39 0.1856391319091344 1101 40-41 0.23789175367524962 1101 42-43 0.2971710532284959 1101 44-45 0.17769344888154848 1101 46-47 0.019139516173666493 1101 48-49 0.17999647613546443 1101 50-51 0.1372544141228289 1101 52-53 0.25950852582381856 1101 54-55 0.13177563003267068 1101 56-57 0.27759468665288267 1101 58-59 0.32406581727149586 1101 60-61 0.3388087589748565 1101 62-63 0.31602122082464135 1101 64-65 0.29340317727207577 1101 66-67 0.3093450060986598 1101 68-69 0.16802416568939904 1101 70-71 0.05037374856085819 1101 72-73 0.16723549758459555 1101 74-75 0.2771938596647274 1101 76-77 0.24625630074827498 1101 78-79 0.38269088870389467 1101 80-81 0.35011297346258274 1101 82-83 0.5480391826538735 1101 84-85 0.32043130913091744 1101 86-87 -0.017962102152093706 1101 88-89 0.28095468182396743 1101 90-91 0.5167606754201088 1101 92-93 0.39856557488579014 1101 94-95 0.37758177981733 1101 96-97 0.3627003497734229 1101 98-99 0.6639961025199277 1101 100-101 0.7568468806668953 1102 1 0.003963099049254737 1102 2 0.1141009909708437 1102 3 0.19756548084587067 1102 4 0.22220544142026455 1102 5 0.004515741397391082 1102 6 0.15343073421418296 1102 7 0.2400206927702584 1102 8 0.33077763737334465 1102 9 0.2783910693541145 1102 10-11 0.3826878327987231 1102 12-13 0.3430427540531511 1102 14-15 0.40119420303481945 1102 16-17 0.33966149560655623 1102 18-19 0.33898671703659744 1102 20-21 0.3300099056371195 1102 22-23 0.13096818556298473 1102 24-25 0.16930726925755835 1102 26-27 0.38792462405157835 1102 28-29 0.2081882839634588 1102 30-31 0.21540701388122585 1102 32-33 -0.01076280903529181 1102 34-35 0.31658072235543244 1102 36-37 0.17866947649235243 1102 38-39 0.4012160814486663 1102 40-41 0.4817593873182844 1102 42-43 0.5022062518573875 1102 44-45 0.5088674427409998 1102 46-47 0.37554010416441486 1102 48-49 0.52630271330775 1102 50-51 0.5090577240826306 1102 52-53 0.487967612923768 1102 54-55 0.8232759747746528 1102 56-57 0.6426211047364419 1102 58-59 0.36165179344462217 1102 60-61 0.5526590863523211 1102 62-63 0.6771116830118622 1102 64-65 0.7780168369174589 1102 66-67 0.7933157783862157 1102 68-69 0.8842465508379043 1102 70-71 0.5936703555280687 1102 72-73 0.5966715113626258 1102 74-75 0.6389730777302276 1102 76-77 0.777436337603266 1102 78-79 0.6041193509217031 1102 80-81 0.755418869169219 1102 82-83 0.9813874899962585 1102 84-85 0.7414362520100504 1102 86-87 0.5271994606102446 1102 88-89 0.5967991249255249 1102 90-91 0.6991677680038997 1102 92-93 0.5858940684834941 1102 94-95 0.6538609836854299 1102 96-97 1.0353410878634435 1102 98-99 1.0470146297618221 1102 100-101 0.9032220662341821 1103 1 0.21133016860803622 1103 2 0.30494247802387164 1103 3 0.2650412432110265 1103 4 0.11255872460344563 1103 5 0.07559790197866079 1103 6 0.1339132382156336 1103 7 0.2139109905863137 1103 8 0.24926403462912816 1103 9 0.2671349675818675 1103 10-11 0.22416929772285243 1103 12-13 0.29419194293435424 1103 14-15 0.3376780796407388 1103 16-17 0.3701942873902979 1103 18-19 0.3328301244320073 1103 20-21 0.29758528117079663 1103 22-23 0.35723185042314753 1103 24-25 0.37238976181373573 1103 26-27 0.41960815179409394 1103 28-29 0.3526709448458121 1103 30-31 0.5293035683066378 1103 32-33 0.51546926232772 1103 34-35 0.4690022712936397 1103 36-37 0.48323291034640903 1103 38-39 0.3352600252815705 1103 40-41 0.6382374874780581 1103 42-43 0.4371745098614923 1103 44-45 0.5000265411973217 1103 46-47 0.44962659625242907 1103 48-49 0.4708901718794394 1103 50-51 0.2406191723997395 1103 52-53 0.18976184001675023 1103 54-55 0.4557826512681018 1103 56-57 0.2530621605118455 1103 58-59 0.2194983380442821 1103 60-61 0.037000281219825126 1103 62-63 0.5103668863408082 1103 64-65 0.5171895964309243 1103 66-67 0.6581011469854658 1103 68-69 0.5854679452960134 1103 70-71 0.5820025448518678 1103 72-73 0.7223451756836354 1103 74-75 0.3984765061886506 1103 76-77 0.5885735058469699 1103 78-79 0.5916268288944799 1103 80-81 0.6750150229377603 1103 82-83 0.941270164600521 1103 84-85 0.5971702337292868 1103 86-87 0.25022709452015235 1103 88-89 0.6580454940793459 1103 90-91 0.4224054215746307 1103 92-93 0.39744780935327384 1103 94-95 0.6273737221358751 1103 96-97 0.9049917308423332 1103 98-99 0.7333351836135265 1103 100-101 1.1870229947083892 1104 1 0.0025684075629719416 1104 2 0.05438042561481993 1104 3 -0.012485133682712757 1104 4 -0.04136418575489387 1104 5 -0.1681794341125169 1104 6 0.01959554870887814 1104 7 0.08724399659092796 1104 8 3.5716752127257223E-4 1104 9 -0.09993787673064958 1104 10-11 0.06749568671533268 1104 12-13 0.07807207060083954 1104 14-15 0.05182719485878806 1104 16-17 -0.04937436587163546 1104 18-19 0.127006501160011 1104 20-21 0.022325966389956875 1104 22-23 0.12043055221259635 1104 24-25 0.16343320910718262 1104 26-27 0.09666066857901257 1104 28-29 0.13481700720566892 1104 30-31 0.1882774404313281 1104 32-33 -0.014231727033681807 1104 34-35 0.16354734860122022 1104 36-37 0.12427206921933731 1104 38-39 0.10615903152816486 1104 40-41 0.2458122572292396 1104 42-43 0.1504909567621482 1104 44-45 0.18989749910202391 1104 46-47 0.06546118503641196 1104 48-49 0.054583618592808136 1104 50-51 0.25181293401161753 1104 52-53 0.22826243003711966 1104 54-55 0.40984905797775895 1104 56-57 0.22960196244068953 1104 58-59 -0.07409366369926573 1104 60-61 0.13056942174577557 1104 62-63 0.386260048319631 1104 64-65 0.22919766073319892 1104 66-67 -0.02683170505035548 1104 68-69 0.09166155360204087 1104 70-71 0.3444952110312727 1104 72-73 0.2351253421323598 1104 74-75 -0.027718067529921342 1104 76-77 0.29929613163649904 1104 78-79 0.4711952397674253 1104 80-81 0.4262990630525145 1104 82-83 0.5213324896453919 1104 84-85 0.27909891253114694 1104 86-87 0.2525049812693929 1104 88-89 0.2773245442957091 1104 90-91 0.43527011341329214 1104 92-93 0.45454559072752687 1104 94-95 0.5744778677747782 1104 96-97 0.7369831718179221 1104 98-99 0.39078014059673194 1104 100-101 1.0737420878338249 1105 1 0.05258021092337373 1105 2 0.2217711633555588 1105 3 0.14331187396797063 1105 4 0.12000102425716364 1105 5 0.07173099637226699 1105 6 0.14258875804696203 1105 7 0.207357339565867 1105 8 0.2411434161237409 1105 9 0.3001624737721116 1105 10-11 0.21275073583309734 1105 12-13 0.25699615042139357 1105 14-15 0.3550902974664041 1105 16-17 0.08327384967088847 1105 18-19 0.13464243067684123 1105 20-21 0.28112835531990044 1105 22-23 0.24791660757342981 1105 24-25 0.1909537421276326 1105 26-27 0.20060746657744488 1105 28-29 0.21534894385189318 1105 30-31 0.23641425366442803 1105 32-33 0.26427607590899527 1105 34-35 0.29776082575711627 1105 36-37 0.21983338043664702 1105 38-39 0.2704450247568104 1105 40-41 0.29792469674249844 1105 42-43 0.48365808137580046 1105 44-45 0.33122935694217404 1105 46-47 0.22175873853765893 1105 48-49 0.243244264083053 1105 50-51 0.3047727802081326 1105 52-53 0.4889077228218852 1105 54-55 0.6621538396112001 1105 56-57 0.4975016597656676 1105 58-59 0.5464213884373592 1105 60-61 0.27096759648114954 1105 62-63 0.470101149200179 1105 64-65 0.6677199787376722 1105 66-67 0.5925476575857331 1105 68-69 0.5518495100498377 1105 70-71 0.37214589913646634 1105 72-73 0.36622486025687806 1105 74-75 0.4286122962417487 1105 76-77 0.5562460731023613 1105 78-79 0.7396180256062017 1105 80-81 0.7382605621582705 1105 82-83 0.7026933161789977 1105 84-85 0.3091397811180592 1105 86-87 0.36132159785587703 1105 88-89 0.7406265694715373 1105 90-91 0.7784711913365285 1105 92-93 0.8111737589864063 1105 94-95 1.0946694573758897 1105 96-97 1.2336407694962155 1105 98-99 1.2084407650252125 1105 100-101 1.4379519954604625 1106 1 0.07611739783970961 1106 2 0.1663549955475041 1106 3 0.23948195681840545 1106 4 0.29275137146275654 1106 5 0.1414297655883061 1106 6 0.2915158927627317 1106 7 0.1995009150651228 1106 8 0.306120069540718 1106 9 0.33888224294025804 1106 10-11 0.35776588118054065 1106 12-13 0.3987742022388332 1106 14-15 0.5520412935872798 1106 16-17 0.42937095948363435 1106 18-19 0.3377142423716819 1106 20-21 0.3267874473174075 1106 22-23 0.32792307278103294 1106 24-25 0.40932606625004553 1106 26-27 0.6102358368662237 1106 28-29 0.5590026840942244 1106 30-31 0.5596134538943076 1106 32-33 0.5052297538937651 1106 34-35 0.5769345961704104 1106 36-37 0.519292637656136 1106 38-39 0.518021409987405 1106 40-41 0.5100811427939291 1106 42-43 0.7105320936357487 1106 44-45 0.3069047915478009 1106 46-47 0.44093238922488354 1106 48-49 0.4256419581590052 1106 50-51 0.5281910192771377 1106 52-53 0.5272901039339075 1106 54-55 0.8523761709171751 1106 56-57 0.726096913824378 1106 58-59 0.7824527087764253 1106 60-61 0.5344260036455566 1106 62-63 0.5160721276016069 1106 64-65 0.5324128813764304 1106 66-67 0.5663498581508435 1106 68-69 0.4567232589124366 1106 70-71 0.5477627876954436 1106 72-73 0.7633097101203923 1106 74-75 0.6800792398747149 1106 76-77 0.8257237681361218 1106 78-79 0.7526948657958492 1106 80-81 0.9065327952888644 1106 82-83 1.007652609316299 1106 84-85 0.870322734520677 1106 86-87 0.9731781301100781 1106 88-89 1.3428219264946648 1106 90-91 1.1091023871604833 1106 92-93 1.356626742559989 1106 94-95 1.481235534192134 1106 96-97 1.5313548995666153 1106 98-99 1.4119880687941908 1106 100-101 1.5874219681629178 1107 1 0.2942696218249701 1107 2 0.37737331975285926 1107 3 0.3077847924635968 1107 4 0.23658102801186942 1107 5 0.16542219021849291 1107 6 0.21922703060374715 1107 7 0.2277985042548707 1107 8 0.24466654545820887 1107 9 0.3442654901122921 1107 10-11 0.27754162720091813 1107 12-13 0.32971306183856797 1107 14-15 0.5120416134930963 1107 16-17 0.47545716770393653 1107 18-19 0.47048168363696874 1107 20-21 0.4332455780437243 1107 22-23 0.2224639439485614 1107 24-25 0.3386728185661596 1107 26-27 0.4281121740725524 1107 28-29 0.43986598193933446 1107 30-31 0.44168938673967517 1107 32-33 0.4062834596827898 1107 34-35 0.41008873959058434 1107 36-37 0.41264633832665965 1107 38-39 0.3085868755143508 1107 40-41 0.4246607225656476 1107 42-43 0.3491380080545383 1107 44-45 0.28482162883090467 1107 46-47 0.35432722978382714 1107 48-49 0.29231103478284837 1107 50-51 0.13326159050097175 1107 52-53 0.3371707150819816 1107 54-55 0.47418338287398143 1107 56-57 0.4536826485840635 1107 58-59 0.3348973010884535 1107 60-61 0.29136540068706296 1107 62-63 0.5325618650229025 1107 64-65 0.4674595876261094 1107 66-67 0.4098988293337271 1107 68-69 0.21639113269039356 1107 70-71 0.1481386770336215 1107 72-73 0.48593402552193865 1107 74-75 0.4255631294176361 1107 76-77 0.3367754265278933 1107 78-79 0.7221283765702751 1107 80-81 0.7349060102143916 1107 82-83 0.9193158636542229 1107 84-85 0.8317886069678266 1107 86-87 1.1242404093750658 1107 88-89 1.0190208695258391 1107 90-91 1.0215981644036667 1107 92-93 1.1274020023235138 1107 94-95 1.4234290004356716 1107 96-97 1.3253780991366604 1107 98-99 1.49705684854543 1107 100-101 1.80758870307616 1108 1 0.042448104940447706 1108 2 0.09547867623725992 1108 3 0.14900688125228 1108 4 0.12872580285862512 1108 5 0.072137857357788 1108 6 0.09804195688244732 1108 7 0.09990476026605677 1108 8 0.0585343453446967 1108 9 0.07252985703271975 1108 10-11 0.11260031703605478 1108 12-13 0.040109245100936164 1108 14-15 0.12131889380530936 1108 16-17 0.2528569735562769 1108 18-19 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2316 92-93 -2.589183343276922 2316 94-95 -2.9467986472916188 2316 96-97 -2.488253469532097 2316 98-99 -2.8832349538912787 2316 100-101 -3.0446181912484853 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 12.0 4 34.0 5 108.0 6 215.0 7 541.0 8 1362.0 9 2577.0 10 4381.0 11 6809.0 12 9980.0 13 13932.0 14 17818.0 15 24278.0 16 31209.0 17 39583.0 18 34453.0 19 24129.0 20 22495.0 21 23489.0 22 25341.0 23 28552.0 24 33036.0 25 39023.0 26 47236.0 27 57858.0 28 69964.0 29 84586.0 30 101383.0 31 123086.0 32 149776.0 33 185834.0 34 235623.0 35 306337.0 36 399651.0 37 414980.0 38 155258.0 39 3844.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.57957916198073 21.293791614656968 15.880956926956124 21.24567229640617 2 15.806465020526181 22.759324874214844 39.24415985004725 22.19005025521173 3 19.67327076816128 26.783668319990877 30.885530746497032 22.65753016535081 4 13.16736397826227 18.800783441071804 37.99417016855017 30.03768241211575 5 12.703183253344946 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20.272977687965493 26-27 20.93903527662703 26.98162293302594 31.741663230675353 20.337678559671673 28-29 20.63913793417358 27.290781884391535 31.718792844488767 20.35128733694612 30-31 20.974574946437276 26.897095199124603 31.588472749675866 20.539857104762255 32-33 20.889259399845812 26.96969730407302 31.509768395096604 20.631274900984565 34-35 21.245469007181544 26.99037250232182 31.468492243749484 20.295666246747157 36-37 21.2655537176829 26.809590384897614 31.450739924589595 20.47411597282989 38-39 20.872531806629816 27.171213781530284 31.531849667670514 20.424404744169383 40-41 21.144712300087576 27.03465522612757 31.295763167893515 20.52486930589134 42-43 21.002712618734538 27.19991540307675 30.95726935844268 20.840102619746027 44-45 21.26401955312143 27.243648040136133 30.673977631243798 20.818354775498644 46-47 21.582790440375934 27.578723156959327 30.070439993408783 20.76804640925596 48-49 21.509591566917155 27.826789960233832 29.764192868940476 20.899425603908533 50-51 21.42070669908121 27.94544234394003 29.6287781998894 21.005072757089362 52-53 21.644102508270297 28.16476298438336 29.22322112311247 20.967913384233878 54-55 21.494606946143023 28.046307620159816 29.247491977085264 21.211593456611894 56-57 21.551961155913904 28.310706344218968 28.986233278952056 21.151099220915075 58-59 21.769707709843896 28.003057263741592 29.218073166147494 21.00916186026701 60-61 21.469734406508937 28.155080189316855 29.500068506440062 20.875116897734138 62-63 21.316464700633446 27.94371654282408 29.677806982117676 21.062011774424796 64-65 21.391495866516205 28.083211832400806 29.398198892735394 21.12709340834759 66-67 21.393039400656434 28.144022242374277 29.03591305568005 21.42702530128924 68-69 21.40692562671677 28.12227358528041 29.15579165888129 21.315009129121528 70-71 21.58826187409759 27.97853583861346 29.086263264067192 21.34693902322176 72-73 21.50855259649142 27.89161623016333 29.096060159668085 21.503771013677166 74-75 21.327972210339542 28.506618398659068 28.656505716500003 21.50890367450139 76-77 21.413696026223587 28.25058279945299 28.800334268313588 21.53538690600984 78-79 21.439354942765405 28.328011986318057 28.667301881202377 21.56533118971416 80-81 21.470408220314795 28.334666064424248 28.577058830453065 21.617866884807896 82-83 21.58168805959307 28.249494946708488 28.70609806447579 21.462718929222653 84-85 21.413052034083712 28.31991034009412 28.537825548659328 21.729212077162842 86-87 21.271966767276037 28.412743134201325 28.53664125562847 21.778648842894167 88-89 21.468469376420245 28.32016292949408 28.373092210237015 21.838275483848662 90-91 21.349189928799802 28.209181266706945 28.62089734500417 21.820731459489085 92-93 21.475388732057638 28.358562220117516 28.454028008937847 21.712021038887 94-95 21.31787293909941 28.270746935660362 28.37629079272834 22.035089332511884 96-97 21.316497273121 28.222530981409776 28.41532054506316 22.045651200406066 98-99 21.192185612934157 28.32302438487918 28.099613260410234 22.385176741776426 100-101 21.105479581295686 28.468207746989805 28.122254567911632 22.304058103802873 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1093.0 1 800.5 2 588.5 3 703.5 4 656.5 5 675.0 6 1154.0 7 2406.0 8 4097.0 9 4946.0 10 4508.5 11 3913.5 12 3871.5 13 4127.5 14 4496.0 15 4842.5 16 5257.5 17 5726.5 18 6190.0 19 6711.5 20 7411.5 21 8546.5 22 10108.5 23 12225.5 24 14618.5 25 17725.5 26 22628.0 27 27949.0 28 31355.0 29 35450.0 30 41300.0 31 46599.5 32 52970.5 33 60452.0 34 67948.5 35 74524.0 36 82309.5 37 88613.0 38 89551.5 39 89190.0 40 89407.0 41 90612.5 42 90499.5 43 90676.5 44 93212.5 45 95289.5 46 95938.5 47 98384.0 48 105950.5 49 104406.0 50 93307.5 51 85229.5 52 80853.5 53 78114.0 54 73727.5 55 66645.5 56 58954.0 57 52624.5 58 47660.0 59 42297.5 60 34962.0 61 27999.0 62 21482.0 63 15582.5 64 10858.0 65 7612.0 66 5463.0 67 3911.5 68 2926.0 69 2168.5 70 1512.0 71 945.5 72 607.5 73 462.5 74 302.0 75 181.0 76 132.0 77 76.5 78 36.5 79 29.0 80 22.5 81 8.0 82 3.5 83 2.5 84 2.0 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.019567636159667497 2 0.0019126260907945673 3 3.678127097681861E-4 4 0.0 5 0.0017287197359104746 6 0.0 7 2.5746889683773023E-4 8 0.0 9 3.6781270976818606E-5 10-11 1.8390635488409303E-5 12-13 0.0052413311141966505 14-15 0.002556298332888893 16-17 0.0 18-19 3.678127097681861E-4 20-21 0.0011770006712581954 22-23 0.0011770006712581954 24-25 6.620628775827348E-4 26-27 2.2068762586091164E-4 28-29 1.2873444841886511E-4 30-31 0.0 32-33 2.5746889683773023E-4 34-35 0.0 36-37 0.0019126260907945673 38-39 0.008753942492482828 40-41 8.091879614900092E-4 42-43 0.0 44-45 0.0010114849518625118 46-47 2.5746889683773023E-4 48-49 2.3907826134932092E-4 50-51 0.001342516390653879 52-53 3.310314387913674E-4 54-55 0.0 56-57 3.1264080330295816E-4 58-59 1.4712508390727442E-4 60-61 0.0019310167262829767 62-63 3.6781270976818606E-5 64-65 1.1034381293045582E-4 66-67 1.8390635488409303E-5 68-69 1.1034381293045582E-4 70-71 3.862033452565954E-4 72-73 3.678127097681861E-4 74-75 0.002427563884470028 76-77 3.6781270976818606E-5 78-79 3.1264080330295816E-4 80-81 0.0017287197359104746 82-83 9.195317744204652E-5 84-85 0.00209653244567866 86-87 3.310314387913674E-4 88-89 0.001949407361771386 90-91 0.0011953913067466048 92-93 0.0 94-95 0.0 96-97 2.7585953232613954E-4 98-99 7.540160550247813E-4 100-101 0.0020045792682366137 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2718775.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.40964619682305 #Duplication Level Percentage of deduplicated Percentage of total 1 72.81375889931306 36.70515824375146 2 14.865643758221012 14.98743684679864 3 5.404270760490404 8.172821309644714 4 2.4318377380905356 4.903523199409053 5 1.253573591345952 3.159610061071514 6 0.7621335321722158 2.305132902692187 7 0.49790419434296046 1.756942199372 8 0.3231761517219055 1.3032956374041649 9 0.24217965729146831 1.0987371756117696 >10 1.2206559428540367 11.370153810972985 >50 0.11271183177383474 3.944346929769329 >100 0.06667102589952699 6.211233808260824 >500 0.0037283832083579624 1.2301281273795177 >1k 0.001462111062091978 1.1300005623021483 >5k 1.4621110620919778E-4 0.565976865793836 >10k+ 1.4621110620919778E-4 1.1555023197659675 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17179 0.6318654541107668 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14176 0.5214112973673806 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9541 0.3509301063898263 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5817 0.21395665327215382 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3839 0.14120329928000663 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.6781270976818606E-5 0.0 3 0.0 0.0 0.0 3.6781270976818606E-5 0.0 4 0.0 0.0 0.0 7.356254195363721E-5 0.0 5 0.0 0.0 0.0 7.356254195363721E-5 0.0 6 0.0 0.0 0.0 7.356254195363721E-5 0.0 7 0.0 0.0 0.0 7.356254195363721E-5 0.0 8 0.0 0.0 0.0 7.356254195363721E-5 0.0 9 0.0 0.0 0.0 1.1034381293045582E-4 0.0 10-11 0.0 0.0 0.0 1.1034381293045582E-4 0.0 12-13 0.0 0.0 0.0 1.1034381293045582E-4 0.0 14-15 0.0 0.0 0.0 1.1034381293045582E-4 0.0 16-17 0.0 0.0 0.0 2.0229699037250233E-4 0.0 18-19 0.0 0.0 0.0 2.5746889683773023E-4 0.0 20-21 0.0 0.0 0.0 3.494220742797767E-4 0.0 22-23 0.0 0.0 0.0 5.517190646522791E-4 0.0 24-25 0.0 0.0 0.0 6.252816066059163E-4 0.0 26-27 0.0 0.0 0.0 9.563130453972837E-4 0.0 28-29 0.0 0.0 0.0 0.002078141810190251 0.0 30-31 0.0 0.0 0.0 0.004689612049544372 0.0 32-33 0.0 0.0 0.0 0.011015990657557172 0.0 34-35 0.0 0.0 0.0 0.019604417430644318 0.0 36-37 0.0 0.0 0.0 0.03271694053388015 0.0 38-39 0.0 0.0 0.0 0.051034013480335816 0.0 40-41 0.0 0.0 0.0 0.07266140081470515 0.0 42-43 0.0 0.0 0.0 0.09813243096615204 0.0 44-45 0.0 0.0 0.0 0.12980110527719285 0.0 46-47 3.6781270976818606E-5 0.0 0.0 0.16801684582210738 0.0 48-49 3.6781270976818606E-5 0.0 0.0 0.20902796296126014 0.0 50-51 3.6781270976818606E-5 0.0 0.0 0.25125286204264785 0.0 52-53 3.6781270976818606E-5 0.0 0.0 0.2984616233413946 0.0 54-55 3.6781270976818606E-5 0.0 0.0 0.3541852488712748 0.0 56-57 3.6781270976818606E-5 0.0 0.0 0.4196375205745234 0.0 58-59 3.6781270976818606E-5 0.0 0.0 0.4920230618569025 0.0 60-61 3.6781270976818606E-5 0.0 0.0 0.5752774687129314 0.0 62-63 3.6781270976818606E-5 0.0 0.0 0.6593410635304502 0.0 64-65 3.6781270976818606E-5 0.0 0.0 0.7495103493301211 0.0 66-67 3.6781270976818606E-5 0.0 0.0 0.8604242719607176 0.0 68-69 3.6781270976818606E-5 0.0 0.0 0.9880368916147897 0.0 70-71 3.6781270976818606E-5 0.0 0.0 1.1181138564243087 0.0 72-73 3.6781270976818606E-5 0.0 0.0 1.2629952828019972 0.0 74-75 3.6781270976818606E-5 0.0 0.0 1.4196834971632444 0.0 76-77 3.6781270976818606E-5 0.0 0.0 1.5897600941600536 0.0 78-79 3.6781270976818606E-5 0.0 0.0 1.7838732517402138 0.0 80-81 3.6781270976818606E-5 0.0 0.0 1.9972230140412501 0.0 82-83 3.6781270976818606E-5 0.0 0.0 2.2142325128044797 0.0 84-85 3.6781270976818606E-5 0.0 0.0 2.4437476436998278 0.0 86-87 7.356254195363721E-5 0.0 0.0 2.6921499572417726 0.0 88-89 7.356254195363721E-5 0.0 0.0 2.9635405651442284 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16190 0.0 28.611366 1 GTATCAA 23160 0.0 27.344654 1 ATCAACG 28135 0.0 22.43732 3 GTACATG 15250 0.0 22.33727 1 TCAACGC 28280 0.0 22.322279 4 TATCAAC 28270 0.0 22.29739 2 TACATGG 15155 0.0 22.066103 2 CAACGCA 28620 0.0 22.040497 5 GAGTACT 15735 0.0 21.8716 12-13 AACGCAG 29240 0.0 21.590591 6 AGTACTT 16295 0.0 21.35316 12-13 ACATGGG 15380 0.0 21.032152 3 GTACTTT 16425 0.0 20.778126 14-15 AGAGTAC 25055 0.0 19.89816 10-11 ACGCAGA 32530 0.0 19.33396 7 CGCAGAG 32635 0.0 19.184772 8 CATGGGG 6235 0.0 18.74095 4 GCAGAGT 33530 0.0 18.219326 9 CATGGGA 8885 0.0 18.176674 4 ACTTTTT 19095 0.0 18.034466 16-17 >>END_MODULE