FastQCFastQC Report
Thu 26 May 2016
SRR936759_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936759_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1636159
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108650.6640552660224343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83770.5119918051974166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50560.3090164219981065No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22450.13721160351775102No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21670.1324443406783815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA149650.032.6942251
GGTATCA98000.030.730791
ATCAACG182950.026.6134993
TCAACGC184600.026.3772344
TATCAAC184900.026.3585172
CAACGCA185600.026.158335
AACGCAG189900.025.5925946
GTACATG129700.025.197591
TACATGG130400.024.7344762
GAGTACT95650.023.6172412-13
ACATGGG133550.023.0484453
CGCAGAG211450.022.9155128
ACGCAGA211400.022.8759887
GCAGAGT215900.021.9398029
AGTACTT98000.021.71778912-13
GTACTTT104900.021.53567914-15
TACCTGG12200.021.025412
GTATAGG5000.020.9008141
CATGGGA79300.020.4875494
CATGGGG46900.019.7514444
Produced by FastQC (version 0.11.5)