##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936759_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1636159 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.27373806579923 31.0 31.0 34.0 28.0 34.0 2 31.32116132967517 31.0 31.0 34.0 28.0 34.0 3 31.304728330192848 31.0 31.0 34.0 28.0 34.0 4 34.96408539756833 37.0 35.0 37.0 32.0 37.0 5 34.98391904454274 37.0 35.0 37.0 32.0 37.0 6 34.995343362106006 37.0 35.0 37.0 32.0 37.0 7 34.908703860688355 37.0 35.0 37.0 32.0 37.0 8 34.86200302048884 37.0 35.0 37.0 32.0 37.0 9 36.31478236528357 39.0 37.0 39.0 32.0 39.0 10-11 36.178326189569596 39.0 35.0 39.0 31.5 39.0 12-13 36.05800628178557 39.0 35.0 39.0 30.5 39.0 14-15 37.06686941794777 39.0 36.0 41.0 31.0 41.0 16-17 36.96924748756081 39.0 36.0 41.0 31.0 41.0 18-19 36.98135450161017 39.0 36.0 41.0 31.0 41.0 20-21 36.87302150952322 39.0 36.0 41.0 30.5 41.0 22-23 36.95469969605644 39.0 36.0 41.0 31.0 41.0 24-25 36.98131691357625 39.0 36.0 41.0 31.0 41.0 26-27 36.87054375522183 39.0 36.0 41.0 30.0 41.0 28-29 36.70892896106063 39.0 36.0 41.0 30.0 41.0 30-31 36.431312299110296 39.0 35.5 41.0 30.0 41.0 32-33 36.17171436272392 39.0 35.0 40.0 30.0 41.0 34-35 35.95133877575468 38.0 35.0 40.0 29.0 41.0 36-37 35.70325866862572 38.0 35.0 40.0 27.5 41.0 38-39 35.4688905540354 38.0 35.0 40.0 27.0 41.0 40-41 35.21114757184357 38.0 34.5 40.0 26.0 41.0 42-43 34.90161072365217 38.0 34.0 40.0 25.0 41.0 44-45 34.523721716532435 38.0 33.5 40.0 24.0 41.0 46-47 34.158427756715575 38.0 33.0 40.0 22.5 41.0 48-49 33.886948334483385 38.0 33.0 40.0 20.0 41.0 50-51 32.88938300006295 37.0 32.0 39.5 17.0 40.5 52-53 32.96681587791896 37.5 32.5 39.5 18.0 40.5 54-55 33.37386617070835 38.0 33.0 40.0 17.5 41.0 56-57 33.26906156431007 38.0 33.0 40.0 14.5 41.0 58-59 33.03065655599487 37.0 32.5 40.0 13.5 41.0 60-61 32.7373296238324 37.0 32.0 40.0 11.0 41.0 62-63 32.36560474868274 36.0 31.5 39.5 8.0 41.0 64-65 32.00327504845189 36.0 31.0 39.0 7.5 41.0 66-67 31.554910311283926 35.0 31.0 39.0 6.5 40.5 68-69 31.16378634350329 35.0 30.5 38.5 2.0 40.0 70-71 30.776972775873247 35.0 30.0 38.0 2.0 40.0 72-73 30.388007828090057 35.0 30.0 37.0 2.0 39.5 74-75 29.961738131807486 34.5 29.0 37.0 2.0 39.0 76-77 29.505312136534407 34.0 29.0 36.0 2.0 39.0 78-79 29.024961204870678 34.0 28.5 36.0 2.0 38.0 80-81 28.566308653376595 34.0 27.5 35.0 2.0 37.0 82-83 28.161536562155632 34.0 27.0 35.0 2.0 37.0 84-85 27.777650888452772 34.0 26.0 35.0 2.0 36.0 86-87 27.4009601145121 33.5 26.0 35.0 2.0 36.0 88-89 26.978697058170997 33.0 25.0 35.0 2.0 35.5 90-91 26.55622253093984 33.0 25.0 35.0 2.0 35.0 92-93 26.10528408302616 33.0 24.0 35.0 2.0 35.0 94-95 25.596411473457042 32.5 21.5 35.0 2.0 35.0 96-97 24.9887058653835 32.0 18.0 35.0 2.0 35.0 98-99 24.211934475805833 32.0 2.0 34.5 2.0 35.0 100-101 21.35415934514922 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5249067346711449 1101 2 0.4434433125158854 1101 3 0.5339394200600225 1101 4 0.5484795952968398 1101 5 0.47165841807170494 1101 6 0.6410478364289673 1101 7 0.47116632539602676 1101 8 0.649096611606268 1101 9 0.5764052515114599 1101 10-11 0.8336069115905573 1101 12-13 0.9204898030409012 1101 14-15 1.0345717162459422 1101 16-17 1.0276448935473042 1101 18-19 0.9269027680960917 1101 20-21 0.9420289393590551 1101 22-23 0.9727127603347157 1101 24-25 0.7640816174287366 1101 26-27 0.8605521270288463 1101 28-29 0.9080308327686808 1101 30-31 0.7976414183657212 1101 32-33 0.6040710942066738 1101 34-35 0.6921161477654465 1101 36-37 0.735252297514279 1101 38-39 0.5706620955794293 1101 40-41 0.4192938266380466 1101 42-43 0.5685107247718335 1101 44-45 0.48027532526164407 1101 46-47 0.7365155615169812 1101 48-49 0.9126038796071967 1101 50-51 0.7289320726799602 1101 52-53 0.7929633482445766 1101 54-55 0.6879122237101001 1101 56-57 0.7323643476847295 1101 58-59 0.8099064291704607 1101 60-61 0.7743598602340214 1101 62-63 0.8140201547431971 1101 64-65 0.6943820348574725 1101 66-67 0.8072264195556649 1101 68-69 0.6266361052205234 1101 70-71 0.5696118484206885 1101 72-73 0.5675873735053152 1101 74-75 0.5370779165769335 1101 76-77 0.43979914707161427 1101 78-79 0.411298003735439 1101 80-81 0.5051737651279211 1101 82-83 0.4644717215567802 1101 84-85 0.40816805506136333 1101 86-87 0.4114953616449064 1101 88-89 0.4351400271796031 1101 90-91 0.4161336818639292 1101 92-93 0.45689714877494225 1101 94-95 0.4625035751733755 1101 96-97 0.48664583641038917 1101 98-99 0.7557612035868111 1101 100-101 0.7673895292630597 1102 1 0.2335527309197296 1102 2 0.34173797615132173 1102 3 0.3480185222995864 1102 4 0.2133726691481712 1102 5 0.26438668752882677 1102 6 0.3869822779890839 1102 7 0.39846191882405435 1102 8 0.4296956560159586 1102 9 0.5836103379606712 1102 10-11 0.49789427662282293 1102 12-13 0.6001359444016217 1102 14-15 0.5348237814066792 1102 16-17 0.45192537918926234 1102 18-19 0.4024325617681299 1102 20-21 0.36541779152202736 1102 22-23 0.2632476866768698 1102 24-25 0.26833125330475127 1102 26-27 0.019065103816267026 1102 28-29 0.053281097463973026 1102 30-31 0.2975419896775904 1102 32-33 0.1534215830196075 1102 34-35 0.13244227386251595 1102 36-37 0.015004316991550581 1102 38-39 0.04735063655748917 1102 40-41 0.02327097416334567 1102 42-43 0.014811171394747191 1102 44-45 0.11993769768389484 1102 46-47 0.2805425846220544 1102 48-49 0.40954147601271274 1102 50-51 0.39048434261003706 1102 52-53 0.5592215511113423 1102 54-55 0.4155765094627739 1102 56-57 0.41732320570594084 1102 58-59 0.32368885770272726 1102 60-61 0.26695860367038193 1102 62-63 0.44180751708821475 1102 64-65 0.34291452116587706 1102 66-67 0.29979184094519695 1102 68-69 0.29214724929537894 1102 70-71 0.21467185497760966 1102 72-73 0.11879476025066182 1102 74-75 0.29269295019457786 1102 76-77 0.31301789556003357 1102 78-79 0.2493802661949296 1102 80-81 0.4495151978684433 1102 82-83 0.5626261101240928 1102 84-85 0.1575782010871727 1102 86-87 0.19313921345854013 1102 88-89 0.20998134436786842 1102 90-91 0.3340276210135684 1102 92-93 0.20655022327121486 1102 94-95 0.09961036158443193 1102 96-97 0.14051038766453416 1102 98-99 0.18067050849966293 1102 100-101 0.18960189819186368 1103 1 0.30161502896203984 1103 2 0.49061681624190356 1103 3 0.4059984434078423 1103 4 0.5317720774432004 1103 5 0.4597838691552738 1103 6 0.475794814941068 1103 7 0.5253559984277132 1103 8 0.5362647508567591 1103 9 0.3041037679869234 1103 10-11 0.4169320393938847 1103 12-13 0.2325152278314917 1103 14-15 0.67316541192843 1103 16-17 0.7315237269856354 1103 18-19 0.7937720142990585 1103 20-21 0.7522570883855977 1103 22-23 0.4178338227832086 1103 24-25 0.6031524690921586 1103 26-27 0.584785968752108 1103 28-29 0.5709254261184995 1103 30-31 0.442544019606963 1103 32-33 0.5537140213045149 1103 34-35 0.7598246520473211 1103 36-37 0.785996000872899 1103 38-39 0.639860653523705 1103 40-41 0.4817972014931229 1103 42-43 0.5736705032048732 1103 44-45 0.8668846061572353 1103 46-47 1.1439264785776047 1103 48-49 1.0432516223805877 1103 50-51 1.0952239319118817 1103 52-53 1.0933838595990935 1103 54-55 0.9588461189016613 1103 56-57 0.9789264070786459 1103 58-59 0.6851837849075295 1103 60-61 0.9169583839444684 1103 62-63 0.9690182048269307 1103 64-65 1.0631020251167236 1103 66-67 1.1384543727082708 1103 68-69 0.9827358138800939 1103 70-71 0.8107760807801228 1103 72-73 0.6823873262132274 1103 74-75 0.7586254618882222 1103 76-77 0.7495083183836719 1103 78-79 0.7660331239332159 1103 80-81 0.8844863005308099 1103 82-83 0.6854665847185366 1103 84-85 0.8471533221645977 1103 86-87 0.8207413175538569 1103 88-89 0.7332029927110852 1103 90-91 0.8199758109960271 1103 92-93 0.685077762195661 1103 94-95 1.0144931073875831 1103 96-97 0.9644811999506615 1103 98-99 0.8693597961178341 1103 100-101 0.9607377560744972 1104 1 0.21076644282856094 1104 2 0.395020511123338 1104 3 0.2274200503045627 1104 4 0.3371688432572242 1104 5 0.4376372758857414 1104 6 0.42827469695191667 1104 7 0.4086540241992367 1104 8 0.5032134121311174 1104 9 0.5160166924180984 1104 10-11 0.715947154757437 1104 12-13 0.6969254717498288 1104 14-15 0.5040954350696936 1104 16-17 0.42810731272976454 1104 18-19 0.2899018263270534 1104 20-21 0.26923283764799777 1104 22-23 0.3781607355312744 1104 24-25 0.4009973767167949 1104 26-27 0.22210043766448706 1104 28-29 0.21698874944856783 1104 30-31 0.44467378770848853 1104 32-33 0.43539708385779363 1104 34-35 0.5131948436798623 1104 36-37 0.32120988616225077 1104 38-39 0.3739551127323253 1104 40-41 0.3562804831463069 1104 42-43 0.4416496727417396 1104 44-45 0.2969160668254105 1104 46-47 0.19367353533792198 1104 48-49 0.2541189637844141 1104 50-51 0.30433338269127574 1104 52-53 0.22891564605522774 1104 54-55 0.18217060704184007 1104 56-57 0.1619327211755177 1104 58-59 0.15338917574992905 1104 60-61 0.051863219915723846 1104 62-63 0.23327933170053683 1104 64-65 0.1891192389673968 1104 66-67 0.2788167394396943 1104 68-69 0.1375431663720228 1104 70-71 0.10430704328222618 1104 72-73 0.1780632314145869 1104 74-75 0.1060069452850172 1104 76-77 0.17328591468745813 1104 78-79 0.2266938112759398 1104 80-81 0.17406787430963888 1104 82-83 0.046042147892453755 1104 84-85 0.1550808514805233 1104 86-87 0.24395835223779727 1104 88-89 0.2830131105737834 1104 90-91 0.21802198170626141 1104 92-93 0.05693577188743504 1104 94-95 0.11799334798482164 1104 96-97 0.25810018177509164 1104 98-99 0.4002264586580786 1104 100-101 -0.03765792954777325 1105 1 0.32969048303287707 1105 2 0.28728133702232483 1105 3 0.40752738735703176 1105 4 0.3987209150663418 1105 5 0.11598116245998114 1105 6 0.3525542126797774 1105 7 0.346105475367942 1105 8 0.5546084799621767 1105 9 0.5207519198423967 1105 10-11 0.36492439271143695 1105 12-13 0.3364881595700595 1105 14-15 0.4448844957920173 1105 16-17 0.3722006363337158 1105 18-19 0.35214044726561866 1105 20-21 0.3407006404553954 1105 22-23 -0.044233712444004425 1105 24-25 0.18073476186693682 1105 26-27 -0.02847822517320253 1105 28-29 0.08253987185832301 1105 30-31 0.12394059246961575 1105 32-33 -0.23994167909836506 1105 34-35 -0.020129270279447553 1105 36-37 0.08100007674829612 1105 38-39 0.10698852217453947 1105 40-41 -0.028988330261412898 1105 42-43 -0.21439509810777935 1105 44-45 -0.08554672221177384 1105 46-47 0.19982403475337662 1105 48-49 0.31789055085061335 1105 50-51 0.23097756977787753 1105 52-53 0.31241767431750844 1105 54-55 0.20605957439349254 1105 56-57 0.027294552182432597 1105 58-59 0.21886438544165543 1105 60-61 0.0842908968688576 1105 62-63 0.2990026798633352 1105 64-65 0.08347011657334136 1105 66-67 0.27056245733469453 1105 68-69 0.33478480812675926 1105 70-71 0.2364922703142156 1105 72-73 0.11042735353402477 1105 74-75 0.2553058805453965 1105 76-77 0.05916869917379941 1105 78-79 0.24412529367281977 1105 80-81 0.24512570920830257 1105 82-83 0.11340242533676204 1105 84-85 -0.019719460801251643 1105 86-87 -0.22143074457740042 1105 88-89 -0.04061045650010442 1105 90-91 -0.0739732477580759 1105 92-93 -0.24499284269564825 1105 94-95 -0.03479717999107734 1105 96-97 -0.13773266116601235 1105 98-99 0.2762229356802557 1105 100-101 0.49715403445351214 1106 1 0.20420215022747001 1106 2 0.26875234375465595 1106 3 0.34973442538288424 1106 4 0.42014093909960337 1106 5 0.16069535264455226 1106 6 0.43511199558599145 1106 7 0.5100306906868823 1106 8 0.4397666821935431 1106 9 0.4867244877313439 1106 10-11 0.4554281108284002 1106 12-13 0.6728715303610997 1106 14-15 0.4451270909823464 1106 16-17 0.4029159527633226 1106 18-19 0.22310997328741422 1106 20-21 0.17157659763797284 1106 22-23 0.27935684720927867 1106 24-25 0.3148364808989683 1106 26-27 0.23546974130089637 1106 28-29 0.24328683878995605 1106 30-31 0.6117259874849736 1106 32-33 0.3713573490125057 1106 34-35 0.4909320648237028 1106 36-37 0.3427156646833893 1106 38-39 0.3930786418883514 1106 40-41 0.455818674580442 1106 42-43 0.3984418326734058 1106 44-45 0.1858387351492894 1106 46-47 0.3895246748024448 1106 48-49 0.41227704898275164 1106 50-51 0.07931535278465418 1106 52-53 0.09192064328041027 1106 54-55 0.44269924134674454 1106 56-57 0.41165332155861734 1106 58-59 0.5247838337826991 1106 60-61 0.45918342594256956 1106 62-63 0.6958720706351684 1106 64-65 0.7449109308887252 1106 66-67 0.6512439612740373 1106 68-69 0.5506140814473248 1106 70-71 0.3333763676338535 1106 72-73 0.1677772336190948 1106 74-75 0.5995478271392614 1106 76-77 0.5288625264460265 1106 78-79 0.2994476813122837 1106 80-81 0.5829596232617291 1106 82-83 0.452872285712548 1106 84-85 0.47364436662180864 1106 86-87 0.6605096147148792 1106 88-89 0.5720737524384596 1106 90-91 0.5348074411619841 1106 92-93 0.7568222218371865 1106 94-95 0.7596863562171983 1106 96-97 0.45955178106616756 1106 98-99 0.5567867872371401 1106 100-101 0.23929294471089335 1107 1 0.16302381636620922 1107 2 0.3332171472438503 1107 3 0.3131527144109292 1107 4 0.4364672956698641 1107 5 0.3202285024216067 1107 6 0.3401959360357836 1107 7 0.4773194994391048 1107 8 0.5681005434542357 1107 9 0.538037808556858 1107 10-11 0.48194427758846814 1107 12-13 0.35147158883920326 1107 14-15 0.2676387256891033 1107 16-17 0.19564822049558472 1107 18-19 0.3020968400791588 1107 20-21 0.5008436720269955 1107 22-23 0.4843159125341927 1107 24-25 0.4582552572445735 1107 26-27 0.39878934852294634 1107 28-29 0.533189618936639 1107 30-31 0.4185289406293933 1107 32-33 0.08143020298780357 1107 34-35 0.3020885912241269 1107 36-37 0.37791704866526743 1107 38-39 0.3544331410477284 1107 40-41 0.47344495074329984 1107 42-43 0.5990010295740618 1107 44-45 0.4697341080690549 1107 46-47 0.47153169217531854 1107 48-49 0.4405183199069569 1107 50-51 0.26683575920749547 1107 52-53 0.3615455305882236 1107 54-55 0.5364755869523634 1107 56-57 0.3541827433563398 1107 58-59 0.4601996374911934 1107 60-61 0.5603505514999441 1107 62-63 0.5612737421343965 1107 64-65 0.493159633405714 1107 66-67 0.5693807025815083 1107 68-69 0.6206081118072078 1107 70-71 0.49511341107821494 1107 72-73 0.4116047475685569 1107 74-75 0.6074033862143295 1107 76-77 0.5114537157445334 1107 78-79 0.3230935476410757 1107 80-81 0.3620671011496981 1107 82-83 0.44492169971318063 1107 84-85 0.28881882630846434 1107 86-87 0.44309044851522117 1107 88-89 0.261364949046726 1107 90-91 0.3334094489103343 1107 92-93 0.28054788641650674 1107 94-95 0.3668773359691535 1107 96-97 0.21627919998870837 1107 98-99 0.45506472880776627 1107 100-101 0.21844306288539528 1108 1 0.21096711820056058 1108 2 0.16575659223465422 1108 3 0.2752449023786063 1108 4 0.41438879051742816 1108 5 0.12151554223004979 1108 6 0.19708865421254984 1108 7 0.19359349781427682 1108 8 0.16743350621132436 1108 9 0.2341553097327278 1108 10-11 0.3132554761175683 1108 12-13 0.10603715945536152 1108 14-15 0.2801446050237786 1108 16-17 0.37260871136658125 1108 18-19 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-1.2357405480467705 2316 72-73 -0.9849855779937293 2316 74-75 -1.0960567829000425 2316 76-77 -1.1424917157709906 2316 78-79 -1.9619643072765918 2316 80-81 -1.9390086922158147 2316 82-83 -1.8623324168867335 2316 84-85 -1.5550310932423912 2316 86-87 -1.7216214750752066 2316 88-89 -2.0335136925608843 2316 90-91 -2.3472476665598627 2316 92-93 -2.9368094447258706 2316 94-95 -2.8548747437268496 2316 96-97 -3.048568802476119 2316 98-99 -4.157691595864446 2316 100-101 -5.043206658110115 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4139.0 3 1769.0 4 1855.0 5 2100.0 6 2374.0 7 2971.0 8 3641.0 9 4526.0 10 5606.0 11 7277.0 12 9654.0 13 11355.0 14 12676.0 15 15621.0 16 19426.0 17 25159.0 18 19149.0 19 13381.0 20 13136.0 21 13684.0 22 15225.0 23 16582.0 24 18830.0 25 21471.0 26 24804.0 27 28766.0 28 33828.0 29 40369.0 30 48363.0 31 59187.0 32 71712.0 33 91150.0 34 117798.0 35 157488.0 36 210337.0 37 262887.0 38 199117.0 39 28740.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.236727154029445 18.912621169313677 14.653341760731179 21.197309915925704 2 15.521066063542033 20.76959518315326 40.468664517016364 23.24067423628834 3 19.33087433430976 26.713128462934073 29.7640559057544 24.191941297001765 4 12.43129638697396 17.103600555314088 37.76331418098813 32.70178887672382 5 13.119738330327394 38.46256839788463 33.18815149940391 15.229541772384067 6 29.876741906467668 36.625515409860526 18.243322984879683 15.254419698792123 7 27.81469906721196 34.42526357481344 20.385339684352008 17.374697673622595 8 27.168868676037157 33.07134608177646 19.91920293598532 19.840582306201064 9 27.850158385440643 16.558967012662887 20.581302997676033 35.00957160422043 10-11 24.879455234265897 27.10346489007589 26.570444856960474 21.446635018697737 12-13 26.74067953546623 24.961491115368307 27.307239303486693 20.99059004567877 14-15 23.647367486321677 25.50329108150994 26.987663144136008 23.861678288032373 16-17 21.439899294659106 29.095602211457983 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21.421828611069092 42-43 21.62846723381341 26.408813236724633 30.123871963825827 21.838847565636126 44-45 21.679372470019175 26.522175207456133 29.94635508569312 21.85209723683157 46-47 22.131663549573997 26.774389722150914 29.27396188590218 21.819984842372904 48-49 22.14738827521589 27.02636875428218 28.729614379200815 22.096628591301116 50-51 22.158255880511227 27.303773874941285 28.529979918752087 22.007990325795404 52-53 22.373911924691114 27.38039537592251 28.335630988176984 21.910061711209387 54-55 21.982076528650722 27.345243196030484 28.44388402186463 22.228796253454167 56-57 22.19190038083282 27.63730242502067 27.952340584701535 22.218456609444974 58-59 22.421589897445788 27.113100577209355 28.14564122436718 22.319668300977682 60-61 22.08581420074329 27.267512558030372 28.665620075001318 21.981053166225017 62-63 22.222459908082804 27.304034204761003 28.581823204706915 21.89168268244928 64-65 21.974712463832603 27.364311899578396 28.483560717835005 22.177414918753996 66-67 22.010661673572823 27.518622473384784 28.134323271650164 22.33639258139223 68-69 22.124758510563165 27.433566901784157 28.2965543787282 22.145120208924478 70-71 22.112502155670608 27.396066099893467 28.175470975806345 22.31596076862958 72-73 22.100667891684914 27.498847724852588 28.087027643600383 22.31345673986212 74-75 21.971174039761337 28.017545758185484 27.867846166168075 22.143434035885104 76-77 22.058000439568637 27.6891864587183 27.939722240308324 22.313090861404746 78-79 22.06192262485002 27.757549220218664 27.910129757669083 22.270398397262234 80-81 22.046099682312672 28.095524575578356 27.663387843099713 22.19498789900926 82-83 22.127990563381186 28.016062798276682 27.62311494641912 22.232831691923014 84-85 21.987680954322578 27.959777264416285 27.638658035836 22.413883745425135 86-87 22.105184973260855 27.87879843795467 27.496020305611186 22.519996283173292 88-89 22.160545601709774 27.748732708470374 27.360353398884442 22.730368290935413 90-91 22.005729257428317 27.70105138294002 27.65002613935486 22.643193220276803 92-93 22.168188932091066 27.7393441420292 27.713606240676885 22.37886068520285 94-95 21.873619859535513 27.54858934935766 27.8049879288808 22.77280286222603 96-97 21.933141405215803 27.426566710812782 27.76794771909866 22.872344164872754 98-99 21.753168231737018 27.72148117528105 27.461137203260616 23.06421338972132 100-101 21.918382987286275 27.566544982141828 27.599389711893004 22.915682318678897 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 819.0 1 608.5 2 711.5 3 941.5 4 749.0 5 712.5 6 1054.5 7 1873.5 8 3013.5 9 3593.0 10 3145.5 11 2570.5 12 2506.0 13 2708.0 14 2968.0 15 3174.5 16 3272.0 17 3406.5 18 3645.0 19 3881.0 20 4177.5 21 4717.5 22 5458.5 23 6329.0 24 7379.5 25 8594.0 26 10640.0 27 13150.5 28 15140.5 29 17729.0 30 21052.5 31 24873.0 32 29626.5 33 33043.0 34 35556.5 35 37773.5 36 41167.0 37 44887.5 38 45714.0 39 46073.5 40 46811.0 41 48508.5 42 49795.5 43 50982.5 44 53131.0 45 55594.5 46 56451.0 47 57325.0 48 63223.5 49 62669.5 50 55631.0 51 51780.0 52 49911.5 53 49657.5 54 48293.0 55 45140.5 56 41441.5 57 37930.5 58 36023.5 59 33132.5 60 28483.0 61 24411.0 62 21139.0 63 16503.0 64 11856.0 65 9453.0 66 7368.0 67 5745.5 68 4300.5 69 3053.5 70 2397.5 71 1999.5 72 1722.5 73 1619.5 74 1312.5 75 1027.0 76 807.5 77 516.5 78 338.0 79 213.5 80 136.5 81 94.5 82 65.5 83 42.5 84 23.5 85 17.5 86 11.5 87 10.5 88 9.0 89 4.5 90 3.5 91 3.5 92 3.5 93 1.5 94 2.0 95 1.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013751719728950548 2 0.014301788518108571 3 0.01607423239428442 4 0.020046951427092355 5 0.03110944596460368 6 0.04950619102422197 7 0.005867400417685568 8 0.027870151984006445 9 0.03618230257572766 10-11 0.040766209152044514 12-13 0.030406580289568434 14-15 0.027533998835076543 16-17 0.030314902158042098 18-19 0.0253642830556199 20-21 0.05549582895060933 22-23 0.01323221031696797 24-25 0.0026281064370883267 26-27 0.0012834938413687179 28-29 1.2223750870178266E-4 30-31 0.002964259586018229 32-33 0.002169715779456642 34-35 0.0 36-37 1.83356263052674E-4 38-39 0.0 40-41 0.00161964699029862 42-43 0.004553347199141404 44-45 0.0018335626305267397 46-47 0.0011612563326669353 48-49 0.0011001375783160439 50-51 0.0033920908664744685 52-53 0.004308872181737838 54-55 0.005622925400282002 56-57 4.889500348071306E-4 58-59 0.005959078549211905 60-61 0.003330972112123577 62-63 7.639844293861417E-4 64-65 0.0010390188239651525 66-67 0.018305066928091952 68-69 0.044952843825080566 70-71 0.05726827282678518 72-73 0.0161964699029862 74-75 0.033615314892990224 76-77 0.02838966139598902 78-79 0.058490647913803 80-81 0.08406884660965101 82-83 0.050575769225362575 84-85 0.01943576388358344 86-87 0.021697157794566418 88-89 0.023652957933794943 90-91 0.04241641551951858 92-93 0.02683113316004129 94-95 0.014026754123529559 96-97 0.018549541945495518 98-99 0.05027017545360811 100-101 0.0729452333177888 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1636159.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.14033117521561 #Duplication Level Percentage of deduplicated Percentage of total 1 77.73900883241257 42.8655469225224 2 12.821003483433246 14.139087561502043 3 4.086352173648497 6.759684364606209 4 1.7815729839030399 3.9294609738092277 5 0.932587589185833 2.5711594258801376 6 0.5561665955971301 1.8400326161890777 7 0.3699726981907243 1.4280291972817243 8 0.25248606239930227 1.1137732078258946 9 0.18129077976040237 0.8996790271501496 >10 1.1154741524225178 11.569616096508144 >50 0.10779230573200918 4.135997530348172 >100 0.05184256865512173 5.295014309972351 >500 0.0032260866281993124 1.1955265807540292 >1k 8.899549319170518E-4 0.7669404227382055 >5k 2.2248873297926294E-4 0.8239870989876281 >10k+ 1.1124436648963147E-4 0.6664646639247063 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10865 0.6640552660224343 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8377 0.5119918051974166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5056 0.3090164219981065 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2245 0.13721160351775102 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2167 0.1324443406783815 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.7503439457901097E-4 14-15 0.0 0.0 0.0 0.0 3.055937717544566E-4 16-17 0.0 6.111875435089133E-5 0.0 0.0 3.055937717544566E-4 18-19 0.0 6.111875435089133E-5 0.0 3.0559377175445665E-5 3.055937717544566E-4 20-21 0.0 6.111875435089133E-5 0.0 9.1678131526337E-5 3.055937717544566E-4 22-23 0.0 6.111875435089133E-5 0.0 1.2223750870178266E-4 3.055937717544566E-4 24-25 0.0 6.111875435089133E-5 0.0 3.055937717544566E-4 3.055937717544566E-4 26-27 0.0 6.111875435089133E-5 0.0 4.278312804562393E-4 3.055937717544566E-4 28-29 0.0 6.111875435089133E-5 0.0 9.473406924388156E-4 3.055937717544566E-4 30-31 0.0 6.111875435089133E-5 0.0 0.0018641220077021854 3.055937717544566E-4 32-33 0.0 6.111875435089133E-5 0.0 0.0033004127349481314 3.055937717544566E-4 34-35 0.0 6.111875435089133E-5 0.0 0.004950619102422197 3.055937717544566E-4 36-37 0.0 6.111875435089133E-5 0.0 0.0080982349514931 3.055937717544566E-4 38-39 0.0 6.111875435089133E-5 0.0 0.012559904019108167 3.055937717544566E-4 40-41 0.0 6.111875435089133E-5 0.0 0.01720492934977591 3.055937717544566E-4 42-43 0.0 6.111875435089133E-5 0.0 0.021330445268461073 3.055937717544566E-4 44-45 0.0 6.111875435089133E-5 0.0 0.024875333020812768 3.055937717544566E-4 46-47 0.0 6.111875435089133E-5 0.0 0.03022322402651576 3.055937717544566E-4 48-49 0.0 6.111875435089133E-5 0.0 0.0352044025061134 3.055937717544566E-4 50-51 0.0 6.111875435089133E-5 0.0 0.04003278409983382 3.055937717544566E-4 52-53 0.0 6.111875435089133E-5 0.0 0.05143143178627505 3.055937717544566E-4 54-55 0.0 6.111875435089133E-5 0.0 0.0697976174687179 3.055937717544566E-4 56-57 0.0 6.111875435089133E-5 0.0 0.09977636647783009 3.055937717544566E-4 58-59 0.0 6.111875435089133E-5 0.0 0.13782279106125994 3.055937717544566E-4 60-61 0.0 1.2223750870178266E-4 0.0 0.18347850056137577 3.055937717544566E-4 62-63 0.0 1.2223750870178266E-4 0.0 0.21312109642155805 3.055937717544566E-4 64-65 0.0 1.2223750870178266E-4 0.0 0.24789766764721521 3.055937717544566E-4 66-67 0.0 1.2223750870178266E-4 0.0 0.3109416627601596 3.055937717544566E-4 68-69 0.0 1.2223750870178266E-4 0.0 0.4006945535244435 3.055937717544566E-4 70-71 0.0 1.2223750870178266E-4 0.0 0.48864444103537613 3.055937717544566E-4 72-73 0.0 1.2223750870178266E-4 0.0 0.5858538198304688 3.361531489299023E-4 74-75 0.0 1.2223750870178266E-4 0.0 0.6804045328112978 3.66712526105348E-4 76-77 0.0 1.2223750870178266E-4 0.0 0.7672848421210897 3.66712526105348E-4 78-79 0.0 1.2223750870178266E-4 0.0 0.8508341793187582 3.66712526105348E-4 80-81 0.0 1.2223750870178266E-4 0.0 0.9300440849575133 3.66712526105348E-4 82-83 0.0 1.2223750870178266E-4 0.0 1.0435416117871186 3.66712526105348E-4 84-85 0.0 1.2223750870178266E-4 0.0 1.2215499838340895 3.66712526105348E-4 86-87 0.0 1.2223750870178266E-4 0.0 1.4479949687041418 3.972719032807936E-4 88-89 0.0 1.2223750870178266E-4 0.0 1.7163979784360812 4.278312804562393E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 14965 0.0 32.694225 1 GGTATCA 9800 0.0 30.73079 1 ATCAACG 18295 0.0 26.613499 3 TCAACGC 18460 0.0 26.377234 4 TATCAAC 18490 0.0 26.358517 2 CAACGCA 18560 0.0 26.15833 5 AACGCAG 18990 0.0 25.592594 6 GTACATG 12970 0.0 25.19759 1 TACATGG 13040 0.0 24.734476 2 GAGTACT 9565 0.0 23.61724 12-13 ACATGGG 13355 0.0 23.048445 3 CGCAGAG 21145 0.0 22.915512 8 ACGCAGA 21140 0.0 22.875988 7 GCAGAGT 21590 0.0 21.939802 9 AGTACTT 9800 0.0 21.717789 12-13 GTACTTT 10490 0.0 21.535679 14-15 TACCTGG 1220 0.0 21.02541 2 GTATAGG 500 0.0 20.900814 1 CATGGGA 7930 0.0 20.487549 4 CATGGGG 4690 0.0 19.751444 4 >>END_MODULE